Click here to switch to the map view.

The map label for this gene is cca

Identifier: 15927038

GI number: 15927038

Start: 1496703

End: 1497905

Strand: Reverse

Name: cca

Synonym: SA1290

Alternate gene names: 15927038

Gene position: 1497905-1496703 (Counterclockwise)

Preceding gene: 15927039

Following gene: 15927037

Centisome position: 53.22

GC content: 31.84

Gene sequence:

>1203_bases
ATGGATAAATCATTATTTGAACAGGCAAGGCCTATATTAGAACAAATTCAAGACAATGGTTTTGAAGCATATTATGTAGG
TGGCTCTGTAAGAGATTATGTCATGGGAAGAAATATTCATGATATAGATATCACAACAAGTGCAACGCCGGATGAAATAG
AATCTATCTTTAGTCATACGATACCTGTAGGTAAAGAACATGGCACCATAAATGTAGTTTTTAATGATGAAAATTATGAA
GTGACAACATTCCGGGCTGAAGAAGATTATGTCGATCACCGTAGACCAAGTGGTGTTACATTTGTTCGGGATTTATACGA
AGATTTGCAACGAAGAGATTTCACGATGAATGCGATAGCAATGGATACAGCATACAAATTGTATGATTATTTTGATGGTC
AACAAGATATTAATAATCGAATAATAAGAACTGTAGGTATAGCTGAGGAACGATTCCAAGAAGATGCTTTACGTATGATT
CGATGTTTAAGATTCCAGTCACAATTATCATTTGATATTGCAATGGAAACATTTGAAGCGATGCGTACACAAATGGCAGA
TATTAAATTTTTATCAATTGAGCGTATAGTGATTGAACTAACTAAATTAATGCGAGGTATTAATGTTGAAGAGAGTTTTA
ATCATTTAAAATCGCTGAAAGCATTTAATTATATGCCATATTTCGAACAACTTGATATGAATCAAATTAATGTAACTGAA
CCAATTGATTTAGAATTGTTGATTGCTATAGTGTCAGTTAAATTTGATATTAATTACTCATTGAAGCCTTTAAAGCTAAG
TAACCGACAAGTAAAAGATATCAATCAATATATTCAAATTATGAATGCATTACCAAGTATTATTACAAAAGAACAATTAA
AAATGTTTGTTTATGATTATGATACGAATCTCATTAAAAATGTAATGGTTGCAGCAGACGTGTTAAAGGCAAATGATATT
CAAGGACATGAACCATTAATCGTTAACCTTCAAACGATTGATGAAACATTACATCGTTTACCTATGCATAATAGAAAAGA
CATGATGGTTAATGGCGGTGTACTTATGGCACATTTAAATGCCAAAAGTGGTCCGTGGTTAAAAGATGTGCTAAGACAAA
TTGAGATAGCGATTGTAACAGGTAAAGTAAGCAACGAAGAAACTGAAATTTTGAAATGGGTGGATAATCATGTCAAAATA
TAG

Upstream 100 bases:

>100_bases
ATTGCTGAACGTTTTGGATCAGAACTTATTACAGATCAATATGAGTATTATTATCAAAAAATGCTAAATGAGCATAACAA
AAGTAAAGGCGAGTAATAAT

Downstream 100 bases:

>100_bases
TCAAGATGTACTTCAATTACTCTATAAAAATAAACCGAATTATATATCTGGACAAAGCATTGCGGAATCACTTAATATTT
CACGCACTGCAGTAAAAAAG

Product: tRNA CCA-pyrophosphorylase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MDKSLFEQARPILEQIQDNGFEAYYVGGSVRDYVMGRNIHDIDITTSATPDEIESIFSHTIPVGKEHGTINVVFNDENYE
VTTFRAEEDYVDHRRPSGVTFVRDLYEDLQRRDFTMNAIAMDTAYKLYDYFDGQQDINNRIIRTVGIAEERFQEDALRMI
RCLRFQSQLSFDIAMETFEAMRTQMADIKFLSIERIVIELTKLMRGINVEESFNHLKSLKAFNYMPYFEQLDMNQINVTE
PIDLELLIAIVSVKFDINYSLKPLKLSNRQVKDINQYIQIMNALPSIITKEQLKMFVYDYDTNLIKNVMVAADVLKANDI
QGHEPLIVNLQTIDETLHRLPMHNRKDMMVNGGVLMAHLNAKSGPWLKDVLRQIEIAIVTGKVSNEETEILKWVDNHVKI

Sequences:

>Translated_400_residues
MDKSLFEQARPILEQIQDNGFEAYYVGGSVRDYVMGRNIHDIDITTSATPDEIESIFSHTIPVGKEHGTINVVFNDENYE
VTTFRAEEDYVDHRRPSGVTFVRDLYEDLQRRDFTMNAIAMDTAYKLYDYFDGQQDINNRIIRTVGIAEERFQEDALRMI
RCLRFQSQLSFDIAMETFEAMRTQMADIKFLSIERIVIELTKLMRGINVEESFNHLKSLKAFNYMPYFEQLDMNQINVTE
PIDLELLIAIVSVKFDINYSLKPLKLSNRQVKDINQYIQIMNALPSIITKEQLKMFVYDYDTNLIKNVMVAADVLKANDI
QGHEPLIVNLQTIDETLHRLPMHNRKDMMVNGGVLMAHLNAKSGPWLKDVLRQIEIAIVTGKVSNEETEILKWVDNHVKI
>Mature_400_residues
MDKSLFEQARPILEQIQDNGFEAYYVGGSVRDYVMGRNIHDIDITTSATPDEIESIFSHTIPVGKEHGTINVVFNDENYE
VTTFRAEEDYVDHRRPSGVTFVRDLYEDLQRRDFTMNAIAMDTAYKLYDYFDGQQDINNRIIRTVGIAEERFQEDALRMI
RCLRFQSQLSFDIAMETFEAMRTQMADIKFLSIERIVIELTKLMRGINVEESFNHLKSLKAFNYMPYFEQLDMNQINVTE
PIDLELLIAIVSVKFDINYSLKPLKLSNRQVKDINQYIQIMNALPSIITKEQLKMFVYDYDTNLIKNVMVAADVLKANDI
QGHEPLIVNLQTIDETLHRLPMHNRKDMMVNGGVLMAHLNAKSGPWLKDVLRQIEIAIVTGKVSNEETEILKWVDNHVKI

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily

Homologues:

Organism=Homo sapiens, GI155030240, Length=276, Percent_Identity=33.695652173913, Blast_Score=125, Evalue=6e-29,
Organism=Escherichia coli, GI87081691, Length=237, Percent_Identity=31.6455696202532, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1789436, Length=225, Percent_Identity=29.7777777777778, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71995920, Length=199, Percent_Identity=35.678391959799, Blast_Score=105, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6321016, Length=209, Percent_Identity=29.6650717703349, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI281360112, Length=228, Percent_Identity=33.7719298245614, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI21357337, Length=228, Percent_Identity=33.7719298245614, Blast_Score=106, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCA_STAA1 (A7X2F4)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001442035.1
- ProteinModelPortal:   A7X2F4
- SMR:   A7X2F4
- STRING:   A7X2F4
- EnsemblBacteria:   EBSTAT00000003293
- GeneID:   5559836
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_1445
- eggNOG:   COG0617
- GeneTree:   EBGT00050000024930
- HOGENOM:   HBG635063
- OMA:   GKASERF
- ProtClustDB:   PRK13299
- BioCyc:   SAUR418127:SAHV_1445-MONOMER
- HAMAP:   MF_01263
- InterPro:   IPR023068
- InterPro:   IPR002646

Pfam domain/function: PF01743 PolyA_pol

EC number: =2.7.7.21; =2.7.7.25

Molecular weight: Translated: 46450; Mature: 46450

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: BINDING 28-28 BINDING 31-31 BINDING 112-112 BINDING 155-155 BINDING 158-158 BINDING 161-161 BINDING 164-164

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKSLFEQARPILEQIQDNGFEAYYVGGSVRDYVMGRNIHDIDITTSATPDEIESIFSHT
CCHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHCCCCEEEEEECCCCHHHHHHHHHHC
IPVGKEHGTINVVFNDENYEVTTFRAEEDYVDHRRPSGVTFVRDLYEDLQRRDFTMNAIA
CCCCCCCCEEEEEECCCCEEEEEEECCHHHHHCCCCCCHHHHHHHHHHHHHCCCEEHHHH
MDTAYKLYDYFDGQQDINNRIIRTVGIAEERFQEDALRMIRCLRFQSQLSFDIAMETFEA
HHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MRTQMADIKFLSIERIVIELTKLMRGINVEESFNHLKSLKAFNYMPYFEQLDMNQINVTE
HHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCC
PIDLELLIAIVSVKFDINYSLKPLKLSNRQVKDINQYIQIMNALPSIITKEQLKMFVYDY
CCCHHHHEEEHHEEEECCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEC
DTNLIKNVMVAADVLKANDIQGHEPLIVNLQTIDETLHRLPMHNRKDMMVNGGVLMAHLN
CHHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHCCCCCCCCEEEECCEEEEEEC
AKSGPWLKDVLRQIEIAIVTGKVSNEETEILKWVDNHVKI
CCCCCHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MDKSLFEQARPILEQIQDNGFEAYYVGGSVRDYVMGRNIHDIDITTSATPDEIESIFSHT
CCHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHCCCCEEEEEECCCCHHHHHHHHHHC
IPVGKEHGTINVVFNDENYEVTTFRAEEDYVDHRRPSGVTFVRDLYEDLQRRDFTMNAIA
CCCCCCCCEEEEEECCCCEEEEEEECCHHHHHCCCCCCHHHHHHHHHHHHHCCCEEHHHH
MDTAYKLYDYFDGQQDINNRIIRTVGIAEERFQEDALRMIRCLRFQSQLSFDIAMETFEA
HHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MRTQMADIKFLSIERIVIELTKLMRGINVEESFNHLKSLKAFNYMPYFEQLDMNQINVTE
HHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCC
PIDLELLIAIVSVKFDINYSLKPLKLSNRQVKDINQYIQIMNALPSIITKEQLKMFVYDY
CCCHHHHEEEHHEEEECCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEC
DTNLIKNVMVAADVLKANDIQGHEPLIVNLQTIDETLHRLPMHNRKDMMVNGGVLMAHLN
CHHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHCCCCCCCCEEEECCEEEEEEC
AKSGPWLKDVLRQIEIAIVTGKVSNEETEILKWVDNHVKI
CCCCCHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA