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The map label for this gene is alsT

Identifier: 15926936

GI number: 15926936

Start: 1358977

End: 1360437

Strand: Direct

Name: alsT

Synonym: SA1190

Alternate gene names: 15926936

Gene position: 1358977-1360437 (Clockwise)

Preceding gene: 15926935

Following gene: 15926937

Centisome position: 48.28

GC content: 35.52

Gene sequence:

>1461_bases
TTGAAAGATTTCGATAGTTTAATTCCTGGATGGTTTAAAGAATTTGTCCATGTTGGTACCGATTTAATATGGTCTCAATA
TTTAATTGGTCTATTATTGACAGCTGGATTCTTCTTTACAATTAGTTCTAAATTCGTTCAATTACGAATGTTACCTGAAA
TGTTTAGAGCTTTAGTAGAACGTCCAGAAACTTTAGAAGATGGTAAGAAGGGTATTTCGCCATTCCAAGCATTTGCGATT
AGTGCTGGTTCGAGAGTTGGTACTGGTAATATTGCTGGTGTTGCGACTGCGATTGTTTTAGGCGGTCCAGGTGCAGTGTT
TTGGATGTGGGTTATTGCATTTATAGGTGCAGCGAGTGCATTTATAGAAGCGACTTTGGCTCAGGTTTATAAAGTACATG
ATAAAGATGGTGGATTCCGTGGTGGTCCAGCTTACTATATTACTAAAGGTTTAAATCAAAAATGGCTAGGTATCGTATTT
GCGATTTTAATTACAATTACATTTGCATTTGTATTTAACACAGTGCAATCTAATACAATTGCGGAGTCGTTAAATACGCA
ATATAATATTAGTCCAGTAATCACAGGTATTATTTTAGCAATCGTAACAGCTATTATTATATTTGGTGGTGTACGTAGTA
TTGCTACGTTATCTTCGTTAATTGTACCGATTATGGCTATCATTTACATTGGTATGGTTTTAGTAATATTGCTATTTAAT
TTAGATCAAATTGTTCCTATGATAGGTACGATTATTAAAAGTGCATTTGGTATCGAACAAGTAACTGGTGGCGCTGTAGG
TGCTGCGGTTCTTCAAGGTATCAAACGTGGTTTATTCTCTAACGAAGCTGGTATGGGTTCTGCGCCGAATGCAGCGGCAA
CTGCTGCCGTACCACACCCTGTTAAGCAAGGTTTAATCCAATCATTAGGTGTGTTCTTTGATACAATGTTGGTTTGTACA
GCAACTGCAATCATGATTTTACTATATTCAGGATTGAAATTTGGTGATAACGCACCTCAAGGTGTTGCAGTTACTCAATC
AGCACTTAATGAGCATTTAGGTTCTGCTGGAGGTATTTTCTTAACAATAGCAGTTACACTGTTTGCATTTTCATCTGTTG
TAGGTAATTACTATTACGGTCAATCTAATATTGAATTTTTATCAACAAATCGTGTGATATTATTTATCTTTAGATGTCTT
GTTGTAGTACTTGTCTTTGTCGGTGCAGTTGTAAAAACAGAAACAGTATGGAATACGGCAGACTTATTTATGGGCTTAAT
GGCAATTGTAAACATTATTTCCATTATAGGACTGTCCAATGTAGCTTTTGCATTGATGAAAGATTATCAAAAGCAGAAAA
AAGAAGGCAAGAACCCTGTCTTTAAACCTGAAAACTTAGAAATTAACTTATTTGGAATTAGTGCTTGGGGCGCTAACAAA
TATAAGAACTCTGATAAATAA

Upstream 100 bases:

>100_bases
TATAAGAAATGATGATGTATCTCTATATTTACTGAAGTTTCAATTCGGTCTAATTTTATAGAAGGTGCATGATTCATGTA
GAGGATGCGAGGGGTTTATT

Downstream 100 bases:

>100_bases
ATATTTTTAATGTAACGATTCATTATATAAAAATCAAATAAACCCTGAGGTTTTCAAATTAAAGCGAAAATCTCAGGGTT
TTAAATTTGTACTCAATAAT

Product: amino acid carrier protein (sodium/alanine symporter)

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MKDFDSLIPGWFKEFVHVGTDLIWSQYLIGLLLTAGFFFTISSKFVQLRMLPEMFRALVERPETLEDGKKGISPFQAFAI
SAGSRVGTGNIAGVATAIVLGGPGAVFWMWVIAFIGAASAFIEATLAQVYKVHDKDGGFRGGPAYYITKGLNQKWLGIVF
AILITITFAFVFNTVQSNTIAESLNTQYNISPVITGIILAIVTAIIIFGGVRSIATLSSLIVPIMAIIYIGMVLVILLFN
LDQIVPMIGTIIKSAFGIEQVTGGAVGAAVLQGIKRGLFSNEAGMGSAPNAAATAAVPHPVKQGLIQSLGVFFDTMLVCT
ATAIMILLYSGLKFGDNAPQGVAVTQSALNEHLGSAGGIFLTIAVTLFAFSSVVGNYYYGQSNIEFLSTNRVILFIFRCL
VVVLVFVGAVVKTETVWNTADLFMGLMAIVNIISIIGLSNVAFALMKDYQKQKKEGKNPVFKPENLEINLFGISAWGANK
YKNSDK

Sequences:

>Translated_486_residues
MKDFDSLIPGWFKEFVHVGTDLIWSQYLIGLLLTAGFFFTISSKFVQLRMLPEMFRALVERPETLEDGKKGISPFQAFAI
SAGSRVGTGNIAGVATAIVLGGPGAVFWMWVIAFIGAASAFIEATLAQVYKVHDKDGGFRGGPAYYITKGLNQKWLGIVF
AILITITFAFVFNTVQSNTIAESLNTQYNISPVITGIILAIVTAIIIFGGVRSIATLSSLIVPIMAIIYIGMVLVILLFN
LDQIVPMIGTIIKSAFGIEQVTGGAVGAAVLQGIKRGLFSNEAGMGSAPNAAATAAVPHPVKQGLIQSLGVFFDTMLVCT
ATAIMILLYSGLKFGDNAPQGVAVTQSALNEHLGSAGGIFLTIAVTLFAFSSVVGNYYYGQSNIEFLSTNRVILFIFRCL
VVVLVFVGAVVKTETVWNTADLFMGLMAIVNIISIIGLSNVAFALMKDYQKQKKEGKNPVFKPENLEINLFGISAWGANK
YKNSDK
>Mature_486_residues
MKDFDSLIPGWFKEFVHVGTDLIWSQYLIGLLLTAGFFFTISSKFVQLRMLPEMFRALVERPETLEDGKKGISPFQAFAI
SAGSRVGTGNIAGVATAIVLGGPGAVFWMWVIAFIGAASAFIEATLAQVYKVHDKDGGFRGGPAYYITKGLNQKWLGIVF
AILITITFAFVFNTVQSNTIAESLNTQYNISPVITGIILAIVTAIIIFGGVRSIATLSSLIVPIMAIIYIGMVLVILLFN
LDQIVPMIGTIIKSAFGIEQVTGGAVGAAVLQGIKRGLFSNEAGMGSAPNAAATAAVPHPVKQGLIQSLGVFFDTMLVCT
ATAIMILLYSGLKFGDNAPQGVAVTQSALNEHLGSAGGIFLTIAVTLFAFSSVVGNYYYGQSNIEFLSTNRVILFIFRCL
VVVLVFVGAVVKTETVWNTADLFMGLMAIVNIISIIGLSNVAFALMKDYQKQKKEGKNPVFKPENLEINLFGISAWGANK
YKNSDK

Specific function: Unknown

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=457, Percent_Identity=40.9190371991247, Blast_Score=335, Evalue=5e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 52142; Mature: 52142

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: PS00873 NA_ALANINE_SYMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDFDSLIPGWFKEFVHVGTDLIWSQYLIGLLLTAGFFFTISSKFVQLRMLPEMFRALVE
CCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RPETLEDGKKGISPFQAFAISAGSRVGTGNIAGVATAIVLGGPGAVFWMWVIAFIGAASA
CCCHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
FIEATLAQVYKVHDKDGGFRGGPAYYITKGLNQKWLGIVFAILITITFAFVFNTVQSNTI
HHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AESLNTQYNISPVITGIILAIVTAIIIFGGVRSIATLSSLIVPIMAIIYIGMVLVILLFN
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDQIVPMIGTIIKSAFGIEQVTGGAVGAAVLQGIKRGLFSNEAGMGSAPNAAATAAVPHP
HHHHHHHHHHHHHHHHCHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCCCHH
VKQGLIQSLGVFFDTMLVCTATAIMILLYSGLKFGDNAPQGVAVTQSALNEHLGSAGGIF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCHH
LTIAVTLFAFSSVVGNYYYGQSNIEFLSTNRVILFIFRCLVVVLVFVGAVVKTETVWNTA
HHHHHHHHHHHHHHCCHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLFMGLMAIVNIISIIGLSNVAFALMKDYQKQKKEGKNPVFKPENLEINLFGISAWGANK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCC
YKNSDK
CCCCCC
>Mature Secondary Structure
MKDFDSLIPGWFKEFVHVGTDLIWSQYLIGLLLTAGFFFTISSKFVQLRMLPEMFRALVE
CCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RPETLEDGKKGISPFQAFAISAGSRVGTGNIAGVATAIVLGGPGAVFWMWVIAFIGAASA
CCCHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
FIEATLAQVYKVHDKDGGFRGGPAYYITKGLNQKWLGIVFAILITITFAFVFNTVQSNTI
HHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AESLNTQYNISPVITGIILAIVTAIIIFGGVRSIATLSSLIVPIMAIIYIGMVLVILLFN
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDQIVPMIGTIIKSAFGIEQVTGGAVGAAVLQGIKRGLFSNEAGMGSAPNAAATAAVPHP
HHHHHHHHHHHHHHHHCHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCCCHH
VKQGLIQSLGVFFDTMLVCTATAIMILLYSGLKFGDNAPQGVAVTQSALNEHLGSAGGIF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCHH
LTIAVTLFAFSSVVGNYYYGQSNIEFLSTNRVILFIFRCLVVVLVFVGAVVKTETVWNTA
HHHHHHHHHHHHHHCCHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLFMGLMAIVNIISIIGLSNVAFALMKDYQKQKKEGKNPVFKPENLEINLFGISAWGANK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCC
YKNSDK
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969507; 9384377; 7710280 [H]