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The map label for this gene is glpF

Identifier: 15926882

GI number: 15926882

Start: 1296691

End: 1297509

Strand: Direct

Name: glpF

Synonym: SA1140

Alternate gene names: 15926882

Gene position: 1296691-1297509 (Clockwise)

Preceding gene: 15926880

Following gene: 15926883

Centisome position: 46.07

GC content: 37.73

Gene sequence:

>819_bases
ATGAATGTATATTTAGCAGAATTCCTAGGAACTGCAATCTTAATCCTTTTTGGTGGTGGCGTTTGTGCCAATGTCAATTT
AAAGAGAAGTGCTGCGAATGGTGCTGATTGGATTGTCATCACAGCTGGATGGGGATTAGCGGTTACAATGGGTGTGTTTG
CTGTCGGTCAATTCTCAGGTGCACATTTAAACCCAGCGGTGTCTTTAGCTCTTGCATTAGACGGAAGTTTTGATTGGTCA
TTAGTTCCTGGTTATATTGTTGCTCAAATGTTAGGTGCAATTGTCGGAGCAACAATTGTATGGTTAATGTACTTGCCACA
TTGGAAAGCGACAGAAGAAGCTGGCGCGAAATTAGGTGTTTTCTCTACAGCACCGGCTATTAAGAATTACTTTGCCAACT
TTTTAAGTGAGATTATCGGAACAATGGCATTAACTTTAGGTATTTTATTTATCGGTGTAAACAAAATTGCCGATGGTTTA
AATCCTTTAATTGTCGGAGCATTAATTGTTGCAATCGGATTAAGTTTAGGCGGTGCTACTGGTTATGCAATCAACCCAGC
ACGTGATTTAGGTCCGAGAATTGCACATGCGATTTTACCAATAGCTGGTAAAGGTGGTTCAAATTGGTCATATGCAATCG
TTCCTATCTTAGGACCAATTGCCGGTGGTTTATTAGGTGCAGTGGTATACGCTGTATTTTATAAACATACATTTAATATT
GGTTGTGCAATTGCAATTGTTGTAGTTATTATTACTTTGATTTTAGGTTACATTTTAAATAAATCATCAAAAAAAGGTGA
TATCGAATCAATTTACTAA

Upstream 100 bases:

>100_bases
AAATATAATAAAAGTTAATACATAGAATAGAGACGGGAGATTTCTACGAGCCAAACTGCTAGTGTAGGAATCTCTTTGTC
TTTTTGGGAGGACATTTAAT

Downstream 100 bases:

>100_bases
AATAAAAAGAAACGTAAATAGCATAATTTAACATGTTTGATTCATGGATTATGCTATTTTTTCGCCAAAATTTAACAGAT
TTTGTACAATGGGTTAGCGA

Product: glycerol uptake facilitator

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MNVYLAEFLGTAILILFGGGVCANVNLKRSAANGADWIVITAGWGLAVTMGVFAVGQFSGAHLNPAVSLALALDGSFDWS
LVPGYIVAQMLGAIVGATIVWLMYLPHWKATEEAGAKLGVFSTAPAIKNYFANFLSEIIGTMALTLGILFIGVNKIADGL
NPLIVGALIVAIGLSLGGATGYAINPARDLGPRIAHAILPIAGKGGSNWSYAIVPILGPIAGGLLGAVVYAVFYKHTFNI
GCAIAIVVVIITLILGYILNKSSKKGDIESIY

Sequences:

>Translated_272_residues
MNVYLAEFLGTAILILFGGGVCANVNLKRSAANGADWIVITAGWGLAVTMGVFAVGQFSGAHLNPAVSLALALDGSFDWS
LVPGYIVAQMLGAIVGATIVWLMYLPHWKATEEAGAKLGVFSTAPAIKNYFANFLSEIIGTMALTLGILFIGVNKIADGL
NPLIVGALIVAIGLSLGGATGYAINPARDLGPRIAHAILPIAGKGGSNWSYAIVPILGPIAGGLLGAVVYAVFYKHTFNI
GCAIAIVVVIITLILGYILNKSSKKGDIESIY
>Mature_272_residues
MNVYLAEFLGTAILILFGGGVCANVNLKRSAANGADWIVITAGWGLAVTMGVFAVGQFSGAHLNPAVSLALALDGSFDWS
LVPGYIVAQMLGAIVGATIVWLMYLPHWKATEEAGAKLGVFSTAPAIKNYFANFLSEIIGTMALTLGILFIGVNKIADGL
NPLIVGALIVAIGLSLGGATGYAINPARDLGPRIAHAILPIAGKGGSNWSYAIVPILGPIAGGLLGAVVYAVFYKHTFNI
GCAIAIVVVIITLILGYILNKSSKKGDIESIY

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI4826645, Length=257, Percent_Identity=38.1322957198444, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI157266307, Length=245, Percent_Identity=37.9591836734694, Blast_Score=124, Evalue=9e-29,
Organism=Homo sapiens, GI22538420, Length=245, Percent_Identity=35.9183673469388, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI4502187, Length=247, Percent_Identity=35.2226720647773, Blast_Score=114, Evalue=7e-26,
Organism=Homo sapiens, GI310133356, Length=166, Percent_Identity=33.1325301204819, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI310114181, Length=166, Percent_Identity=33.1325301204819, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI4755125, Length=245, Percent_Identity=29.3877551020408, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI4502181, Length=245, Percent_Identity=29.3877551020408, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI37694062, Length=233, Percent_Identity=31.3304721030043, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1790362, Length=261, Percent_Identity=37.1647509578544, Blast_Score=122, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17544068, Length=248, Percent_Identity=35.4838709677419, Blast_Score=111, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI71994009, Length=239, Percent_Identity=31.3807531380753, Blast_Score=107, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17533613, Length=237, Percent_Identity=30.8016877637131, Blast_Score=101, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI32564052, Length=237, Percent_Identity=30.8016877637131, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI71992966, Length=244, Percent_Identity=31.1475409836066, Blast_Score=100, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI17531431, Length=236, Percent_Identity=34.3220338983051, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17531429, Length=236, Percent_Identity=34.3220338983051, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71992961, Length=234, Percent_Identity=27.7777777777778, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71993722, Length=237, Percent_Identity=30.379746835443, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17558372, Length=239, Percent_Identity=28.4518828451883, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6321054, Length=206, Percent_Identity=35.9223300970874, Blast_Score=109, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6322985, Length=287, Percent_Identity=29.9651567944251, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24652747, Length=241, Percent_Identity=29.4605809128631, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI45551084, Length=241, Percent_Identity=29.4605809128631, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 28116; Mature: 28116

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVYLAEFLGTAILILFGGGVCANVNLKRSAANGADWIVITAGWGLAVTMGVFAVGQFSG
CCCHHHHHHHHHHHHHCCCCEEECCEEECCCCCCCCEEEEECCCHHHHHHHHHHHHCCCC
AHLNPAVSLALALDGSFDWSLVPGYIVAQMLGAIVGATIVWLMYLPHWKATEEAGAKLGV
CCCCHHHEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCEECC
FSTAPAIKNYFANFLSEIIGTMALTLGILFIGVNKIADGLNPLIVGALIVAIGLSLGGAT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCC
GYAINPARDLGPRIAHAILPIAGKGGSNWSYAIVPILGPIAGGLLGAVVYAVFYKHTFNI
CCCCCCHHHHHHHHHHHHHHEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GCAIAIVVVIITLILGYILNKSSKKGDIESIY
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MNVYLAEFLGTAILILFGGGVCANVNLKRSAANGADWIVITAGWGLAVTMGVFAVGQFSG
CCCHHHHHHHHHHHHHCCCCEEECCEEECCCCCCCCEEEEECCCHHHHHHHHHHHHCCCC
AHLNPAVSLALALDGSFDWSLVPGYIVAQMLGAIVGATIVWLMYLPHWKATEEAGAKLGV
CCCCHHHEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCEECC
FSTAPAIKNYFANFLSEIIGTMALTLGILFIGVNKIADGLNPLIVGALIVAIGLSLGGAT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCC
GYAINPARDLGPRIAHAILPIAGKGGSNWSYAIVPILGPIAGGLLGAVVYAVFYKHTFNI
CCCCCCHHHHHHHHHHHHHHEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GCAIAIVVVIITLILGYILNKSSKKGDIESIY
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]