Click here to switch to the map view.

The map label for this gene is glpK

Identifier: 15926883

GI number: 15926883

Start: 1297638

End: 1299134

Strand: Direct

Name: glpK

Synonym: SA1141

Alternate gene names: 15926883

Gene position: 1297638-1299134 (Clockwise)

Preceding gene: 15926882

Following gene: 15926884

Centisome position: 46.1

GC content: 36.34

Gene sequence:

>1497_bases
ATGGAAAAATATATTTTATCTATAGACCAAGGAACAACAAGCTCAAGAGCGATTTTATTCAATCAAAAAGGGGAAATTGC
AGGGGTAGCACAACGTGAGTTTAAGCAATATTTTCCACAATCAGGTTGGGTTGAACATGATGCAAATGAAATTTGGACAT
CTGTGTTAGCTGTAATGACGGAAGTAATTAATGAAAATGATGTTAGAGCTGATCAAATTGCAGGAATCGGTATTACAAAC
CAACGTGAAACAACGGTTGTTTGGGACAAACATACTGGCCGCCCAATTTATCACGCAATTGTTTGGCAATCACGTCAAAC
ACAATCAATTTGTTCAGAATTAAAACAACAAGGATATGAACAAACATTTAGAGATAAGACAGGATTACTTTTAGATCCGT
ATTTTGCAGGTACAAAAGTTAAATGGATTCTAGACAATGTTGAAGGTGCACGAGAAAAAGCAGAAAATGGCGATCTATTA
TTTGGAACGATTGATACTTGGTTAGTATGGAAATTATCAGGAAAAGCTGCGCATATTACTGATTATTCAAATGCGAGTCG
TACATTAATGTTTAATATCCATGATTTAGAATGGGACGATGAGTTATTAGAACTACTTACAGTACCTAAAAATATGTTGC
CAGAAGTTAAACCTTCAAGTGAAATATATGGTAAGACAATTGATTACCACTTCTATGGTCAAGAAGTACCAATCGCTGGT
GTAGCTGGTGATCAACAAGCAGCATTATTTGGACAAGCTTGCTTCGAATGTGGTGACGTGAAAAACACATATGGAACTGG
TGGCTTCATGTTAATGAATACAGGTGACAAAGCGGTTAAATCTGAAAGTGGTTTATTAACAACAATTGCTTATGGTATTG
ATGGAAAAGTAAATTATGCGCTTGAAGGTTCCATCTTTGTTTCGGGTTCAGCAATCCAATGGTTACGTGATGGATTAAGA
ATGATTAATTCAGCACCACAATCAGAAAGTTATGCGACACGAGTTGACTCTACTGAGGGTGTTTATGTTGTTCCAGCTTT
TGTAGGTTTAGGAACACCATATTGGGATTCTGAAGCACGTGGTGCGATTTTCGGTTTAACACGTGGAACTGAAAAAGAGC
ACTTTATCCGTGCAACTTTAGAATCATTATGTTACCAAACTCGTGACGTTATGGAAGCAATGTCAAAAGACTCTGGTATT
GATGTCCAAAGTTTACGTGTCGATGGTGGTGCAGTTAAAAATAACTTTATTATGCAGTTCCAAGCAGACATTGTTAATAC
TTCTGTTGAAAGACCTGAAATTCAAGAAACTACAGCTTTAGGTGCTGCATTTTTAGCAGGTTTAGCAGTTGGATTCTGGG
AGAGTAAAGATGATATCGCTAAAAACTGGAAATTAGAAGAAAAATTCGATCCGAAAATGGATGAAGGCGAAAGAGAAAAA
TTATATAGAGGTTGGAAAAAAGCTGTTGAAGCAACACAAGTTTTTAAAACAGAATAA

Upstream 100 bases:

>100_bases
TAACATGTTTGATTCATGGATTATGCTATTTTTTCGCCAAAATTTAACAGATTTTGTACAATGGGTTAGCGATTATTTTT
TAATAAAGGAGATACTACTA

Downstream 100 bases:

>100_bases
ACTTGTAGATTAGACTTTTGTATAAACATTGTGATACAATCAATTTAAGTTAATATTTGAATCGAGAAACGAGAGATTTG
TTCGAACATGTACAATTGAA

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MEKYILSIDQGTTSSRAILFNQKGEIAGVAQREFKQYFPQSGWVEHDANEIWTSVLAVMTEVINENDVRADQIAGIGITN
QRETTVVWDKHTGRPIYHAIVWQSRQTQSICSELKQQGYEQTFRDKTGLLLDPYFAGTKVKWILDNVEGAREKAENGDLL
FGTIDTWLVWKLSGKAAHITDYSNASRTLMFNIHDLEWDDELLELLTVPKNMLPEVKPSSEIYGKTIDYHFYGQEVPIAG
VAGDQQAALFGQACFECGDVKNTYGTGGFMLMNTGDKAVKSESGLLTTIAYGIDGKVNYALEGSIFVSGSAIQWLRDGLR
MINSAPQSESYATRVDSTEGVYVVPAFVGLGTPYWDSEARGAIFGLTRGTEKEHFIRATLESLCYQTRDVMEAMSKDSGI
DVQSLRVDGGAVKNNFIMQFQADIVNTSVERPEIQETTALGAAFLAGLAVGFWESKDDIAKNWKLEEKFDPKMDEGEREK
LYRGWKKAVEATQVFKTE

Sequences:

>Translated_498_residues
MEKYILSIDQGTTSSRAILFNQKGEIAGVAQREFKQYFPQSGWVEHDANEIWTSVLAVMTEVINENDVRADQIAGIGITN
QRETTVVWDKHTGRPIYHAIVWQSRQTQSICSELKQQGYEQTFRDKTGLLLDPYFAGTKVKWILDNVEGAREKAENGDLL
FGTIDTWLVWKLSGKAAHITDYSNASRTLMFNIHDLEWDDELLELLTVPKNMLPEVKPSSEIYGKTIDYHFYGQEVPIAG
VAGDQQAALFGQACFECGDVKNTYGTGGFMLMNTGDKAVKSESGLLTTIAYGIDGKVNYALEGSIFVSGSAIQWLRDGLR
MINSAPQSESYATRVDSTEGVYVVPAFVGLGTPYWDSEARGAIFGLTRGTEKEHFIRATLESLCYQTRDVMEAMSKDSGI
DVQSLRVDGGAVKNNFIMQFQADIVNTSVERPEIQETTALGAAFLAGLAVGFWESKDDIAKNWKLEEKFDPKMDEGEREK
LYRGWKKAVEATQVFKTE
>Mature_498_residues
MEKYILSIDQGTTSSRAILFNQKGEIAGVAQREFKQYFPQSGWVEHDANEIWTSVLAVMTEVINENDVRADQIAGIGITN
QRETTVVWDKHTGRPIYHAIVWQSRQTQSICSELKQQGYEQTFRDKTGLLLDPYFAGTKVKWILDNVEGAREKAENGDLL
FGTIDTWLVWKLSGKAAHITDYSNASRTLMFNIHDLEWDDELLELLTVPKNMLPEVKPSSEIYGKTIDYHFYGQEVPIAG
VAGDQQAALFGQACFECGDVKNTYGTGGFMLMNTGDKAVKSESGLLTTIAYGIDGKVNYALEGSIFVSGSAIQWLRDGLR
MINSAPQSESYATRVDSTEGVYVVPAFVGLGTPYWDSEARGAIFGLTRGTEKEHFIRATLESLCYQTRDVMEAMSKDSGI
DVQSLRVDGGAVKNNFIMQFQADIVNTSVERPEIQETTALGAAFLAGLAVGFWESKDDIAKNWKLEEKFDPKMDEGEREK
LYRGWKKAVEATQVFKTE

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family

Homologues:

Organism=Homo sapiens, GI189409120, Length=503, Percent_Identity=46.3220675944334, Blast_Score=432, Evalue=1e-121,
Organism=Homo sapiens, GI4504007, Length=503, Percent_Identity=46.3220675944334, Blast_Score=432, Evalue=1e-121,
Organism=Homo sapiens, GI42794763, Length=507, Percent_Identity=45.956607495069, Blast_Score=432, Evalue=1e-121,
Organism=Homo sapiens, GI41393575, Length=496, Percent_Identity=45.1612903225806, Blast_Score=426, Evalue=1e-119,
Organism=Homo sapiens, GI88196792, Length=505, Percent_Identity=32.8712871287129, Blast_Score=321, Evalue=9e-88,
Organism=Escherichia coli, GI1790361, Length=489, Percent_Identity=61.9631901840491, Blast_Score=660, Evalue=0.0,
Organism=Escherichia coli, GI1789987, Length=477, Percent_Identity=24.3186582809224, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1790005, Length=517, Percent_Identity=23.0174081237911, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1787789, Length=508, Percent_Identity=23.6220472440945, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1789168, Length=470, Percent_Identity=21.2765957446809, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17535599, Length=497, Percent_Identity=40.6438631790744, Blast_Score=389, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI71986140, Length=515, Percent_Identity=26.6019417475728, Blast_Score=198, Evalue=6e-51,
Organism=Caenorhabditis elegans, GI71986147, Length=294, Percent_Identity=31.6326530612245, Blast_Score=143, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6321755, Length=545, Percent_Identity=34.3119266055046, Blast_Score=305, Evalue=1e-83,
Organism=Drosophila melanogaster, GI17864214, Length=502, Percent_Identity=42.2310756972112, Blast_Score=390, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24654573, Length=433, Percent_Identity=44.1108545034642, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24655555, Length=493, Percent_Identity=39.1480730223124, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24655560, Length=493, Percent_Identity=39.1480730223124, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24655570, Length=493, Percent_Identity=39.1480730223124, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24655574, Length=493, Percent_Identity=39.1480730223124, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24655565, Length=493, Percent_Identity=39.1480730223124, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI19922058, Length=523, Percent_Identity=30.7839388145315, Blast_Score=266, Evalue=2e-71,
Organism=Drosophila melanogaster, GI24652871, Length=490, Percent_Identity=31.2244897959184, Blast_Score=251, Evalue=8e-67,
Organism=Drosophila melanogaster, GI24656367, Length=495, Percent_Identity=27.8787878787879, Blast_Score=224, Evalue=9e-59,
Organism=Drosophila melanogaster, GI21357867, Length=510, Percent_Identity=27.0588235294118, Blast_Score=218, Evalue=1e-56,
Organism=Drosophila melanogaster, GI24656372, Length=330, Percent_Identity=30.6060606060606, Blast_Score=179, Evalue=3e-45,
Organism=Drosophila melanogaster, GI45552909, Length=290, Percent_Identity=26.8965517241379, Blast_Score=127, Evalue=1e-29,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GLPK_STAA1 (A7X1U3)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001441880.1
- ProteinModelPortal:   A7X1U3
- SMR:   A7X1U3
- STRING:   A7X1U3
- EnsemblBacteria:   EBSTAT00000003069
- GeneID:   5559208
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_1290
- eggNOG:   COG0554
- GeneTree:   EBGT00050000024725
- HOGENOM:   HBG511469
- OMA:   KPSSEVY
- ProtClustDB:   PRK00047
- BioCyc:   SAUR418127:SAHV_1290-MONOMER
- HAMAP:   MF_00186
- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999
- PANTHER:   PTHR10196
- PANTHER:   PTHR10196:SF9
- TIGRFAMs:   TIGR01311

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N

EC number: =2.7.1.30

Molecular weight: Translated: 55613; Mature: 55613

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00933 FGGY_KINASES_1; PS00445 FGGY_KINASES_2

Important sites: BINDING 12-12 BINDING 16-16 BINDING 82-82 BINDING 134-134 BINDING 244-244 BINDING 266-266 BINDING 309-309

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKYILSIDQGTTSSRAILFNQKGEIAGVAQREFKQYFPQSGWVEHDANEIWTSVLAVMT
CCCEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
EVINENDVRADQIAGIGITNQRETTVVWDKHTGRPIYHAIVWQSRQTQSICSELKQQGYE
HHHCCCCCCHHHEECCCCCCCCCEEEEEECCCCCEEEEEEEECCCCHHHHHHHHHHCCHH
QTFRDKTGLLLDPYFAGTKVKWILDNVEGAREKAENGDLLFGTIDTWLVWKLSGKAAHIT
HHHHCCCCCEECCCCCCCEEEEEEECCHHHHHHCCCCCEEEEECCEEEEEEECCCEEEEE
DYSNASRTLMFNIHDLEWDDELLELLTVPKNMLPEVKPSSEIYGKTIDYHFYGQEVPIAG
CCCCCCEEEEEEEECCCCHHHHHHHHHCCHHHCCCCCCCHHHCCCEEEEEEECCCCCEEE
VAGDQQAALFGQACFECGDVKNTYGTGGFMLMNTGDKAVKSESGLLTTIAYGIDGKVNYA
CCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHCCCCEEEEEEECCCCEEEEE
LEGSIFVSGSAIQWLRDGLRMINSAPQSESYATRVDSTEGVYVVPAFVGLGTPYWDSEAR
EECEEEECCCHHHHHHHHHHHHHCCCCCCCHHEECCCCCCEEEEEEHHHCCCCCCCCCCC
GAIFGLTRGTEKEHFIRATLESLCYQTRDVMEAMSKDSGIDVQSLRVDGGAVKNNFIMQF
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEE
QADIVNTSVERPEIQETTALGAAFLAGLAVGFWESKDDIAKNWKLEEKFDPKMDEGEREK
EHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCHHHH
LYRGWKKAVEATQVFKTE
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEKYILSIDQGTTSSRAILFNQKGEIAGVAQREFKQYFPQSGWVEHDANEIWTSVLAVMT
CCCEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
EVINENDVRADQIAGIGITNQRETTVVWDKHTGRPIYHAIVWQSRQTQSICSELKQQGYE
HHHCCCCCCHHHEECCCCCCCCCEEEEEECCCCCEEEEEEEECCCCHHHHHHHHHHCCHH
QTFRDKTGLLLDPYFAGTKVKWILDNVEGAREKAENGDLLFGTIDTWLVWKLSGKAAHIT
HHHHCCCCCEECCCCCCCEEEEEEECCHHHHHHCCCCCEEEEECCEEEEEEECCCEEEEE
DYSNASRTLMFNIHDLEWDDELLELLTVPKNMLPEVKPSSEIYGKTIDYHFYGQEVPIAG
CCCCCCEEEEEEEECCCCHHHHHHHHHCCHHHCCCCCCCHHHCCCEEEEEEECCCCCEEE
VAGDQQAALFGQACFECGDVKNTYGTGGFMLMNTGDKAVKSESGLLTTIAYGIDGKVNYA
CCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHCCCCEEEEEEECCCCEEEEE
LEGSIFVSGSAIQWLRDGLRMINSAPQSESYATRVDSTEGVYVVPAFVGLGTPYWDSEAR
EECEEEECCCHHHHHHHHHHHHHCCCCCCCHHEECCCCCCEEEEEEHHHCCCCCCCCCCC
GAIFGLTRGTEKEHFIRATLESLCYQTRDVMEAMSKDSGIDVQSLRVDGGAVKNNFIMQF
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEE
QADIVNTSVERPEIQETTALGAAFLAGLAVGFWESKDDIAKNWKLEEKFDPKMDEGEREK
EHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCHHHH
LYRGWKKAVEATQVFKTE
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA