Click here to switch to the map view.

The map label for this gene is pnpA

Identifier: 15926857

GI number: 15926857

Start: 1266443

End: 1268539

Strand: Direct

Name: pnpA

Synonym: SA1117

Alternate gene names: 15926857

Gene position: 1266443-1268539 (Clockwise)

Preceding gene: 15926856

Following gene: 15926858

Centisome position: 44.99

GC content: 37.29

Gene sequence:

>2097_bases
ATGTCTCAAGAAAAGAAAGTTTTTAAAACTGAATGGGCAGGAAGATCTTTAACGATTGAAACAGGGCAATTAGCTAAACA
AGCAAATGGCGCTGTATTGGTTCGTTATGGAGATACAGTCGTGTTATCGACGGCAACTGCATCAAAAGAACCTCGTGATG
GAGATTTCTTCCCATTAACAGTGAACTACGAAGAAAAAATGTACGCTGCGGGTAAAATTCCTGGTGGATTTAAAAAGAGA
GAAGGACGTCCTGGTGACGATGCAACATTAACTGCGCGATTAATTGATAGACCAATTAGACCTTTATTCCCTAAAGGGTA
TAAGCATGATGTTCAAATTATGAACATGGTATTAAGTGCAGATCCTGATTGTTCACCACAAATGGCTGCAATGATTGGTT
CATCTATGGCGCTTAGTGTGTCGGATATTCCATTCCAAGGGCCAATCGCCGGTGTAAATGTGGGTTATATTGATGGTAAA
TATATCATTAACCCAACAGTAGAAGAAAAAGAAGTTTCTCGTTTAGACCTTGAAGTAGCTGGTCATAAAGATGCAGTAAA
CATGGTAGAGGCAGGCGCTAGTGAGATTACTGAACAAGAAATGTTAGAGGCGATTTTCTTTGGTCATGAAGAGATTCAAC
GTTTAGTTGATTTCCAACAACAAATCGTCGACCACATTCAACCTGTTAAACAAGAATTTATTCCAGCAGAGCGTGATGAA
GCGCTAGTTGAACGTGTGAAATCTTTAACCGAAGAAAAGGGACTTAAAGAAACAGTTTTAACATTTGATAAACAACAACG
AGATGAAAATCTTGATAACTTAAAAGAAGAAATCGTCAATGAATTTATCGATGAAGAAGATCCAGAGAATGAATTACTTA
TTAAAGAAGTTTATGCAATTTTAAATGAATTAGTGAAAGAAGAAGTTCGACGTTTAATTGCAGATGAAAAAATTAGACCA
GACGGCCGTAAACCTGATGAAATCCGTCCATTAGATTCTGAAGTTGGTATTTTACCTAGAACGCATGGTTCAGGTCTATT
TACACGTGGTCAGACTCAAGCACTTTCAGTTTTAACATTAGGTGCTTTAGGCGATTATCAATTAATTGATGGTTTAGGAC
CTGAAGAAGAAAAAAGATTCATGCATCATTATAACTTCCCGAACTTTTCAGTAGGTGAAACTGGTCCAGTACGTGCGCCA
GGTCGTCGTGAAATTGGACATGGTGCGTTAGGTGAAAGAGCATTAAAATATATTATTCCTGATACTGCTGATTTCCCATA
TACAATTCGTATTGTAAGTGAGGTACTTGAATCAAACGGTTCATCATCTCAAGCGTCAATTTGTGGATCAACCTTAGCAT
TAATGGATGCGGGCGTACCGATTAAAGCACCAGTTGCTGGTATTGCTATGGGCCTTGTTACACGTGAAGATAGCTATACG
ATTTTAACTGATATCCAAGGTATGGAAGATGCATTAGGTGATATGGACTTTAAAGTCGCTGGTACTAAAGAAGGTATTAC
AGCAATCCAAATGGATATTAAAATTGACGGTTTAACGCGTGAAATTATCGAAGAGGCTCTAGAACAAGCGAGACGTGGTC
GTTTAGAAATAATGAATCATATGTTACAAACAATTGATCAACCACGTACTGAATTAAGTGCTTACGCGCCAAAAGTTGTA
ACTATGACAATTAAACCAGATAAGATTAGAGATGTTATCGGACCTGGTGGTAAAAAAATTAACGAAATTATTGATGAAAC
AGGCGTTAAATTAGATATTGAACAAGATGGTACTATCTTTATTGGGGCCGTTGATCAAGCTATGATAAATCGTGCTCGTG
AAATCATTGAGGAAATTACACGTGAAGCGGAAGTAGGTCAAACTTATCAAGCCACTGTTAAACGTATTGAAAAATACGGT
GCGTTTGTAGGCCTATTCCCGGGTAAAGATGCGTTGCTTCACATTTCACAAATTTCAAAAAATAGAATTGAAAAAGTGGA
AGATGTTTTAAAAATCGGTGACACAATTGAAGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCATCACACA
GAGCATTAGAAGAATAA

Upstream 100 bases:

>100_bases
CCCGCTCTCTTTTTTTCCAATTCAATATTTTATAACTAATATTAAAATACGATAATAAATGATATGATATAACTATTAGA
TTCAAGAGAGGAGATTTATA

Downstream 100 bases:

>100_bases
TATTTAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCATTTTTTACGAAGTGATCCATTTTGAATTT
GTTGTATTGAACATTTTAAA

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 698; Mature: 697

Protein sequence:

>698_residues
MSQEKKVFKTEWAGRSLTIETGQLAKQANGAVLVRYGDTVVLSTATASKEPRDGDFFPLTVNYEEKMYAAGKIPGGFKKR
EGRPGDDATLTARLIDRPIRPLFPKGYKHDVQIMNMVLSADPDCSPQMAAMIGSSMALSVSDIPFQGPIAGVNVGYIDGK
YIINPTVEEKEVSRLDLEVAGHKDAVNMVEAGASEITEQEMLEAIFFGHEEIQRLVDFQQQIVDHIQPVKQEFIPAERDE
ALVERVKSLTEEKGLKETVLTFDKQQRDENLDNLKEEIVNEFIDEEDPENELLIKEVYAILNELVKEEVRRLIADEKIRP
DGRKPDEIRPLDSEVGILPRTHGSGLFTRGQTQALSVLTLGALGDYQLIDGLGPEEEKRFMHHYNFPNFSVGETGPVRAP
GRREIGHGALGERALKYIIPDTADFPYTIRIVSEVLESNGSSSQASICGSTLALMDAGVPIKAPVAGIAMGLVTREDSYT
ILTDIQGMEDALGDMDFKVAGTKEGITAIQMDIKIDGLTREIIEEALEQARRGRLEIMNHMLQTIDQPRTELSAYAPKVV
TMTIKPDKIRDVIGPGGKKINEIIDETGVKLDIEQDGTIFIGAVDQAMINRAREIIEEITREAEVGQTYQATVKRIEKYG
AFVGLFPGKDALLHISQISKNRIEKVEDVLKIGDTIEVKITEIDKQGRVNASHRALEE

Sequences:

>Translated_698_residues
MSQEKKVFKTEWAGRSLTIETGQLAKQANGAVLVRYGDTVVLSTATASKEPRDGDFFPLTVNYEEKMYAAGKIPGGFKKR
EGRPGDDATLTARLIDRPIRPLFPKGYKHDVQIMNMVLSADPDCSPQMAAMIGSSMALSVSDIPFQGPIAGVNVGYIDGK
YIINPTVEEKEVSRLDLEVAGHKDAVNMVEAGASEITEQEMLEAIFFGHEEIQRLVDFQQQIVDHIQPVKQEFIPAERDE
ALVERVKSLTEEKGLKETVLTFDKQQRDENLDNLKEEIVNEFIDEEDPENELLIKEVYAILNELVKEEVRRLIADEKIRP
DGRKPDEIRPLDSEVGILPRTHGSGLFTRGQTQALSVLTLGALGDYQLIDGLGPEEEKRFMHHYNFPNFSVGETGPVRAP
GRREIGHGALGERALKYIIPDTADFPYTIRIVSEVLESNGSSSQASICGSTLALMDAGVPIKAPVAGIAMGLVTREDSYT
ILTDIQGMEDALGDMDFKVAGTKEGITAIQMDIKIDGLTREIIEEALEQARRGRLEIMNHMLQTIDQPRTELSAYAPKVV
TMTIKPDKIRDVIGPGGKKINEIIDETGVKLDIEQDGTIFIGAVDQAMINRAREIIEEITREAEVGQTYQATVKRIEKYG
AFVGLFPGKDALLHISQISKNRIEKVEDVLKIGDTIEVKITEIDKQGRVNASHRALEE
>Mature_697_residues
SQEKKVFKTEWAGRSLTIETGQLAKQANGAVLVRYGDTVVLSTATASKEPRDGDFFPLTVNYEEKMYAAGKIPGGFKKRE
GRPGDDATLTARLIDRPIRPLFPKGYKHDVQIMNMVLSADPDCSPQMAAMIGSSMALSVSDIPFQGPIAGVNVGYIDGKY
IINPTVEEKEVSRLDLEVAGHKDAVNMVEAGASEITEQEMLEAIFFGHEEIQRLVDFQQQIVDHIQPVKQEFIPAERDEA
LVERVKSLTEEKGLKETVLTFDKQQRDENLDNLKEEIVNEFIDEEDPENELLIKEVYAILNELVKEEVRRLIADEKIRPD
GRKPDEIRPLDSEVGILPRTHGSGLFTRGQTQALSVLTLGALGDYQLIDGLGPEEEKRFMHHYNFPNFSVGETGPVRAPG
RREIGHGALGERALKYIIPDTADFPYTIRIVSEVLESNGSSSQASICGSTLALMDAGVPIKAPVAGIAMGLVTREDSYTI
LTDIQGMEDALGDMDFKVAGTKEGITAIQMDIKIDGLTREIIEEALEQARRGRLEIMNHMLQTIDQPRTELSAYAPKVVT
MTIKPDKIRDVIGPGGKKINEIIDETGVKLDIEQDGTIFIGAVDQAMINRAREIIEEITREAEVGQTYQATVKRIEKYGA
FVGLFPGKDALLHISQISKNRIEKVEDVLKIGDTIEVKITEIDKQGRVNASHRALEE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=725, Percent_Identity=39.0344827586207, Blast_Score=457, Evalue=1e-128,
Organism=Escherichia coli, GI145693187, Length=687, Percent_Identity=50.0727802037846, Blast_Score=666, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=711, Percent_Identity=34.4585091420534, Blast_Score=367, Evalue=1e-101,
Organism=Drosophila melanogaster, GI281362905, Length=691, Percent_Identity=38.9290882778582, Blast_Score=448, Evalue=1e-126,
Organism=Drosophila melanogaster, GI24651641, Length=691, Percent_Identity=38.9290882778582, Blast_Score=448, Evalue=1e-126,
Organism=Drosophila melanogaster, GI24651643, Length=691, Percent_Identity=38.9290882778582, Blast_Score=448, Evalue=1e-126,
Organism=Drosophila melanogaster, GI161079377, Length=642, Percent_Identity=38.1619937694704, Blast_Score=404, Evalue=1e-112,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 77363; Mature: 77232

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQEKKVFKTEWAGRSLTIETGQLAKQANGAVLVRYGDTVVLSTATASKEPRDGDFFPLT
CCCCCCHHHHHCCCCEEEEEHHHHHHHCCCEEEEEECCEEEEEECCCCCCCCCCCEEEEE
VNYEEKMYAAGKIPGGFKKREGRPGDDATLTARLIDRPIRPLFPKGYKHDVQIMNMVLSA
ECCHHHHEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHC
DPDCSPQMAAMIGSSMALSVSDIPFQGPIAGVNVGYIDGKYIINPTVEEKEVSRLDLEVA
CCCCCHHHHHHHCCCCEEEECCCCCCCCCCCCEEEEECCEEEECCCCCHHHHHHHEEEEC
GHKDAVNMVEAGASEITEQEMLEAIFFGHEEIQRLVDFQQQIVDHIQPVKQEFIPAERDE
CCCHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
ALVERVKSLTEEKGLKETVLTFDKQQRDENLDNLKEEIVNEFIDEEDPENELLIKEVYAI
HHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
LNELVKEEVRRLIADEKIRPDGRKPDEIRPLDSEVGILPRTHGSGLFTRGQTQALSVLTL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCHHHHHHEEE
GALGDYQLIDGLGPEEEKRFMHHYNFPNFSVGETGPVRAPGRREIGHGALGERALKYIIP
CCCCCHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCC
DTADFPYTIRIVSEVLESNGSSSQASICGSTLALMDAGVPIKAPVAGIAMGLVTREDSYT
CCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHEECCCCEE
ILTDIQGMEDALGDMDFKVAGTKEGITAIQMDIKIDGLTREIIEEALEQARRGRLEIMNH
EEECCCCHHHHHCCCCEEEECCCCCCEEEEEEEEECHHHHHHHHHHHHHHHCCHHHHHHH
MLQTIDQPRTELSAYAPKVVTMTIKPDKIRDVIGPGGKKINEIIDETGVKLDIEQDGTIF
HHHHHCCCHHHHHHCCCEEEEEEECCHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCEEE
IGAVDQAMINRAREIIEEITREAEVGQTYQATVKRIEKYGAFVGLFPGKDALLHISQISK
EECHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEECCCCHHEEEHHHHHH
NRIEKVEDVLKIGDTIEVKITEIDKQGRVNASHRALEE
HHHHHHHHHHHCCCEEEEEEEEECCCCCCCHHHHHCCC
>Mature Secondary Structure 
SQEKKVFKTEWAGRSLTIETGQLAKQANGAVLVRYGDTVVLSTATASKEPRDGDFFPLT
CCCCCHHHHHCCCCEEEEEHHHHHHHCCCEEEEEECCEEEEEECCCCCCCCCCCEEEEE
VNYEEKMYAAGKIPGGFKKREGRPGDDATLTARLIDRPIRPLFPKGYKHDVQIMNMVLSA
ECCHHHHEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHC
DPDCSPQMAAMIGSSMALSVSDIPFQGPIAGVNVGYIDGKYIINPTVEEKEVSRLDLEVA
CCCCCHHHHHHHCCCCEEEECCCCCCCCCCCCEEEEECCEEEECCCCCHHHHHHHEEEEC
GHKDAVNMVEAGASEITEQEMLEAIFFGHEEIQRLVDFQQQIVDHIQPVKQEFIPAERDE
CCCHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
ALVERVKSLTEEKGLKETVLTFDKQQRDENLDNLKEEIVNEFIDEEDPENELLIKEVYAI
HHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
LNELVKEEVRRLIADEKIRPDGRKPDEIRPLDSEVGILPRTHGSGLFTRGQTQALSVLTL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCHHHHHHEEE
GALGDYQLIDGLGPEEEKRFMHHYNFPNFSVGETGPVRAPGRREIGHGALGERALKYIIP
CCCCCHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCC
DTADFPYTIRIVSEVLESNGSSSQASICGSTLALMDAGVPIKAPVAGIAMGLVTREDSYT
CCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHEECCCCEE
ILTDIQGMEDALGDMDFKVAGTKEGITAIQMDIKIDGLTREIIEEALEQARRGRLEIMNH
EEECCCCHHHHHCCCCEEEECCCCCCEEEEEEEEECHHHHHHHHHHHHHHHCCHHHHHHH
MLQTIDQPRTELSAYAPKVVTMTIKPDKIRDVIGPGGKKINEIIDETGVKLDIEQDGTIF
HHHHHCCCHHHHHHCCCEEEEEEECCHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCEEE
IGAVDQAMINRAREIIEEITREAEVGQTYQATVKRIEKYGAFVGLFPGKDALLHISQISK
EECHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEECCCCHHEEEHHHHHH
NRIEKVEDVLKIGDTIEVKITEIDKQGRVNASHRALEE
HHHHHHHHHHHCCCEEEEEEEEECCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA