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The map label for this gene is pyrF

Identifier: 15926787

GI number: 15926787

Start: 1186414

End: 1187106

Strand: Direct

Name: pyrF

Synonym: SA1047

Alternate gene names: 15926787

Gene position: 1186414-1187106 (Clockwise)

Preceding gene: 15926786

Following gene: 15926788

Centisome position: 42.15

GC content: 33.91

Gene sequence:

>693_bases
ATGAAAGATTTACCAATTATTGCATTAGATTTTGAATCAAAAGAAAAAGTAAATCAATTTTTAGATTTATTTGATGAATC
ATTATTCGTAAAAGTAGGTATGGAACTTTTTTATCAAGAAGGTCCTCAATTAATTAATGAGATAAAAGAAAGAGGCCATG
ATGTATTTTTAGATTTAAAACTGCATGATATTCCTAATACAGTTGGTAAGGCGATGGAAGGACTAGCTAAATTGAATGTT
GATCTGGTAAATGTTCATGCTGCTGGTGGCGTAAAAATGATGTCTGAGGCCATTAAAGGATTAAGAAAACATAATCAACA
TACAAAAATTATTGCAGTAACACAGCTTACGTCAACAACAGAAGACATGTTACGACACGAACAAAATATACAAACATCGA
TTGAAGAGGCCGTTTTAAATTATGCCAAGTTAGCAAATGCAGCTGGTTTAGATGGCGTTGTTTGTTCACCTCTTGAAAGT
CGTATGTTGACTGAAAAGTTAGGTACATCATTTTTAAAAGTAACACCAGGTATTAGACCTAAAGGTGCATCTCAAGATGA
CCAACACCGTATTACGACACCGGAAGAAGCAAGACAGCTTGGTTCGACGCATATTGTAGTCGGTAGACCGATTACACAAA
GTGACAATCCAGTCGAAAGTTATCATAAAATTAAAGAAAGTTGGTTAGTATAA

Upstream 100 bases:

>100_bases
ACTGTATCGGTGGGGCTGTAATTAACCATTTACTTAAAGAAGTTTATATTACAGCCTCATTATTTTAATGAATTTCTTAA
TATAAAGGGAGACATATATG

Downstream 100 bases:

>100_bases
TGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCAAATGATTTATATACATGGAGTTCA
GGTATTAAATCACCGATTTA

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MKDLPIIALDFESKEKVNQFLDLFDESLFVKVGMELFYQEGPQLINEIKERGHDVFLDLKLHDIPNTVGKAMEGLAKLNV
DLVNVHAAGGVKMMSEAIKGLRKHNQHTKIIAVTQLTSTTEDMLRHEQNIQTSIEEAVLNYAKLANAAGLDGVVCSPLES
RMLTEKLGTSFLKVTPGIRPKGASQDDQHRITTPEEARQLGSTHIVVGRPITQSDNPVESYHKIKESWLV

Sequences:

>Translated_230_residues
MKDLPIIALDFESKEKVNQFLDLFDESLFVKVGMELFYQEGPQLINEIKERGHDVFLDLKLHDIPNTVGKAMEGLAKLNV
DLVNVHAAGGVKMMSEAIKGLRKHNQHTKIIAVTQLTSTTEDMLRHEQNIQTSIEEAVLNYAKLANAAGLDGVVCSPLES
RMLTEKLGTSFLKVTPGIRPKGASQDDQHRITTPEEARQLGSTHIVVGRPITQSDNPVESYHKIKESWLV
>Mature_230_residues
MKDLPIIALDFESKEKVNQFLDLFDESLFVKVGMELFYQEGPQLINEIKERGHDVFLDLKLHDIPNTVGKAMEGLAKLNV
DLVNVHAAGGVKMMSEAIKGLRKHNQHTKIIAVTQLTSTTEDMLRHEQNIQTSIEEAVLNYAKLANAAGLDGVVCSPLES
RMLTEKLGTSFLKVTPGIRPKGASQDDQHRITTPEEARQLGSTHIVVGRPITQSDNPVESYHKIKESWLV

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP)

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787537, Length=225, Percent_Identity=41.3333333333333, Blast_Score=166, Evalue=9e-43,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PYRF_STAA1 (A7X1F5)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001441784.1
- ProteinModelPortal:   A7X1F5
- SMR:   A7X1F5
- STRING:   A7X1F5
- EnsemblBacteria:   EBSTAT00000004737
- GeneID:   5559590
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_1194
- eggNOG:   COG0284
- GeneTree:   EBGT00050000025116
- HOGENOM:   HBG625253
- OMA:   TVHAYPQ
- ProtClustDB:   PRK00230
- BioCyc:   SAUR418127:SAHV_1194-MONOMER
- HAMAP:   MF_01200_B
- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00934
- TIGRFAMs:   TIGR01740

Pfam domain/function: PF00215 OMPdecase; SSF51366 RibP_bind_barrel

EC number: =4.1.1.23

Molecular weight: Translated: 25620; Mature: 25620

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00156 OMPDECASE

Important sites: ACT_SITE 60-60 BINDING 10-10 BINDING 31-31 BINDING 117-117 BINDING 179-179 BINDING 188-188 BINDING 208-208 BINDING 209-209

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDLPIIALDFESKEKVNQFLDLFDESLFVKVGMELFYQEGPQLINEIKERGHDVFLDLK
CCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEEEE
LHDIPNTVGKAMEGLAKLNVDLVNVHAAGGVKMMSEAIKGLRKHNQHTKIIAVTQLTSTT
ECCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEEHHHHH
EDMLRHEQNIQTSIEEAVLNYAKLANAAGLDGVVCSPLESRMLTEKLGTSFLKVTPGIRP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCHHEEECCCCCC
KGASQDDQHRITTPEEARQLGSTHIVVGRPITQSDNPVESYHKIKESWLV
CCCCCCCCCCCCCHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKDLPIIALDFESKEKVNQFLDLFDESLFVKVGMELFYQEGPQLINEIKERGHDVFLDLK
CCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEEEE
LHDIPNTVGKAMEGLAKLNVDLVNVHAAGGVKMMSEAIKGLRKHNQHTKIIAVTQLTSTT
ECCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEEHHHHH
EDMLRHEQNIQTSIEEAVLNYAKLANAAGLDGVVCSPLESRMLTEKLGTSFLKVTPGIRP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCHHEEECCCCCC
KGASQDDQHRITTPEEARQLGSTHIVVGRPITQSDNPVESYHKIKESWLV
CCCCCCCCCCCCCHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA