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The map label for this gene is tuf

Identifier: 15926226

GI number: 15926226

Start: 590790

End: 591974

Strand: Direct

Name: tuf

Synonym: SA0506

Alternate gene names: 15926226

Gene position: 590790-591974 (Clockwise)

Preceding gene: 15926225

Following gene: 161376699

Centisome position: 20.99

GC content: 40.68

Gene sequence:

>1185_bases
ATGGCAAAAGAAAAATTCGATCGTTCTAAAGAACATGCCAATATCGGTACTATCGGTCACGTTGACCATGGTAAAACAAC
ATTAACAGCAGCAATCGCTACTGTATTAGCAAAAAATGGTGACTCAGTTGCACAATCATATGACATGATTGACAACGCTC
CAGAAGAAAAAGAACGTGGTATCACAATCAATACTTCTCACATTGAGTACCAAACTGACAAACGTCACTACGCTCACGTT
GACTGCCCAGGACACGCTGACTACGTTAAAAACATGATCACTGGTGCTGCTCAAATGGACGGCGGTATCTTAGTAGTATC
TGCTGCTGACGGTCCAATGCCACAAACTCGTGAACACATTCTTTTATCACGTAACGTTGGTGTACCAGCATTAGTAGTAT
TCTTAAACAAAGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAA
TATGACTTCCCAGGTGACGATGTACCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGA
AAAAATCTTAGAATTAATGGAAGCTGTAGATACTTACATTCCAACTCCAGAACGTGATTCTGACAAACCATTCATGATGC
CAGTTGAGGACGTATTCTCAATCACTGGTCGTGGTACTGTTGCTACAGGCCGTGTTGAACGTGGTCAAATCAAAGTTGGT
GAAGAAGTTGAAATCATCGGTTTACATGACACATCTAAAACAACTGTTACAGGTGTTGAAATGTTCCGTAAATTATTAGA
CTACGCTGAAGCTGGTGACAACATTGGTGCATTATTACGTGGTGTTGCTCGTGAAGACGTACAACGTGGTCAAGTATTAG
CTGCTCCTGGTTCAATTACACCACATACTGAATTTAAAGCAGAAGTATACGTATTATCAAAAGACGAAGGTGGACGTCAC
ACTCCATTCTTCTCAAACTATCGTCCACAATTCTATTTCCGTACTACTGACGTAACTGGTGTTGTTCACTTACCAGAAGG
TACTGAAATGGTAATGCCTGGTGATAACGTTGAAATGACAGTAGAATTAATCGCTCCAATCGCGATTGAAGACGGTACTC
GTTTCTCAATCCGCGAAGGTGGACGTACTGTAGGATCAGGCGTTGTTACTGAAATCATTAAATAA

Upstream 100 bases:

>100_bases
TCTTTTTGCATCGCTATAAATAATTTCTCATGATGGTGAGAAACTATCATGAGAGATAAATTTAAATATTATTTTTAATT
AGAATAGGAGAGATTTTATA

Downstream 100 bases:

>100_bases
TTTCTAATTTCTTAGATTTTATATAAAAAGAAGATCCCTCAATCGAGGGGTCTTTTTTTAATGTGTAAATTTTGTAATGG
CTATTCGATTTAGAAGAACA

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MAKEKFDRSKEHANIGTIGHVDHGKTTLTAAIATVLAKNGDSVAQSYDMIDNAPEEKERGITINTSHIEYQTDKRHYAHV
DCPGHADYVKNMITGAAQMDGGILVVSAADGPMPQTREHILLSRNVGVPALVVFLNKVDMVDDEELLELVEMEVRDLLSE
YDFPGDDVPVIAGSALKALEGDAQYEEKILELMEAVDTYIPTPERDSDKPFMMPVEDVFSITGRGTVATGRVERGQIKVG
EEVEIIGLHDTSKTTVTGVEMFRKLLDYAEAGDNIGALLRGVAREDVQRGQVLAAPGSITPHTEFKAEVYVLSKDEGGRH
TPFFSNYRPQFYFRTTDVTGVVHLPEGTEMVMPGDNVEMTVELIAPIAIEDGTRFSIREGGRTVGSGVVTEIIK

Sequences:

>Translated_394_residues
MAKEKFDRSKEHANIGTIGHVDHGKTTLTAAIATVLAKNGDSVAQSYDMIDNAPEEKERGITINTSHIEYQTDKRHYAHV
DCPGHADYVKNMITGAAQMDGGILVVSAADGPMPQTREHILLSRNVGVPALVVFLNKVDMVDDEELLELVEMEVRDLLSE
YDFPGDDVPVIAGSALKALEGDAQYEEKILELMEAVDTYIPTPERDSDKPFMMPVEDVFSITGRGTVATGRVERGQIKVG
EEVEIIGLHDTSKTTVTGVEMFRKLLDYAEAGDNIGALLRGVAREDVQRGQVLAAPGSITPHTEFKAEVYVLSKDEGGRH
TPFFSNYRPQFYFRTTDVTGVVHLPEGTEMVMPGDNVEMTVELIAPIAIEDGTRFSIREGGRTVGSGVVTEIIK
>Mature_393_residues
AKEKFDRSKEHANIGTIGHVDHGKTTLTAAIATVLAKNGDSVAQSYDMIDNAPEEKERGITINTSHIEYQTDKRHYAHVD
CPGHADYVKNMITGAAQMDGGILVVSAADGPMPQTREHILLSRNVGVPALVVFLNKVDMVDDEELLELVEMEVRDLLSEY
DFPGDDVPVIAGSALKALEGDAQYEEKILELMEAVDTYIPTPERDSDKPFMMPVEDVFSITGRGTVATGRVERGQIKVGE
EVEIIGLHDTSKTTVTGVEMFRKLLDYAEAGDNIGALLRGVAREDVQRGQVLAAPGSITPHTEFKAEVYVLSKDEGGRHT
PFFSNYRPQFYFRTTDVTGVVHLPEGTEMVMPGDNVEMTVELIAPIAIEDGTRFSIREGGRTVGSGVVTEIIK

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=394, Percent_Identity=56.0913705583756, Blast_Score=470, Evalue=1e-132,
Organism=Homo sapiens, GI223555963, Length=439, Percent_Identity=30.0683371298405, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI5729864, Length=439, Percent_Identity=30.0683371298405, Blast_Score=173, Evalue=3e-43,
Organism=Homo sapiens, GI4503475, Length=450, Percent_Identity=28.8888888888889, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI4503471, Length=448, Percent_Identity=29.0178571428571, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI54607086, Length=328, Percent_Identity=28.6585365853659, Blast_Score=125, Evalue=6e-29,
Organism=Homo sapiens, GI194018522, Length=337, Percent_Identity=27.5964391691395, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI194018520, Length=336, Percent_Identity=27.6785714285714, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI194097354, Length=336, Percent_Identity=27.6785714285714, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI46094014, Length=322, Percent_Identity=27.639751552795, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI4503507, Length=362, Percent_Identity=28.1767955801105, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI157426893, Length=260, Percent_Identity=26.5384615384615, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI94966754, Length=131, Percent_Identity=34.3511450381679, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1790412, Length=393, Percent_Identity=75.0636132315522, Blast_Score=602, Evalue=1e-173,
Organism=Escherichia coli, GI1789737, Length=393, Percent_Identity=75.0636132315522, Blast_Score=602, Evalue=1e-173,
Organism=Escherichia coli, GI2367247, Length=370, Percent_Identity=31.3513513513514, Blast_Score=144, Evalue=8e-36,
Organism=Escherichia coli, GI48994988, Length=272, Percent_Identity=33.4558823529412, Blast_Score=114, Evalue=9e-27,
Organism=Escherichia coli, GI1789108, Length=274, Percent_Identity=31.3868613138686, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI1788922, Length=332, Percent_Identity=24.3975903614458, Blast_Score=62, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=399, Percent_Identity=52.3809523809524, Blast_Score=397, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI25141371, Length=385, Percent_Identity=43.8961038961039, Blast_Score=326, Evalue=1e-89,
Organism=Caenorhabditis elegans, GI17552884, Length=451, Percent_Identity=30.3769401330377, Blast_Score=161, Evalue=7e-40,
Organism=Caenorhabditis elegans, GI17569207, Length=451, Percent_Identity=30.3769401330377, Blast_Score=161, Evalue=7e-40,
Organism=Caenorhabditis elegans, GI32566303, Length=432, Percent_Identity=28.2407407407407, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI115532067, Length=431, Percent_Identity=27.3781902552204, Blast_Score=134, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI115532065, Length=431, Percent_Identity=27.3781902552204, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI32566629, Length=455, Percent_Identity=26.3736263736264, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17506081, Length=370, Percent_Identity=26.7567567567568, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI32566301, Length=154, Percent_Identity=35.0649350649351, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17509919, Length=357, Percent_Identity=26.3305322128852, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17557151, Length=253, Percent_Identity=26.4822134387352, Blast_Score=78, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6324761, Length=396, Percent_Identity=64.6464646464647, Blast_Score=524, Evalue=1e-149,
Organism=Saccharomyces cerevisiae, GI6325337, Length=445, Percent_Identity=28.7640449438202, Blast_Score=152, Evalue=6e-38,
Organism=Saccharomyces cerevisiae, GI6319594, Length=445, Percent_Identity=28.7640449438202, Blast_Score=152, Evalue=6e-38,
Organism=Saccharomyces cerevisiae, GI6320377, Length=339, Percent_Identity=28.3185840707965, Blast_Score=97, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6322937, Length=351, Percent_Identity=24.5014245014245, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320863, Length=362, Percent_Identity=26.2430939226519, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6323320, Length=240, Percent_Identity=27.9166666666667, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323098, Length=179, Percent_Identity=29.608938547486, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=56.4885496183206, Blast_Score=473, Evalue=1e-133,
Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=56.4885496183206, Blast_Score=473, Evalue=1e-133,
Organism=Drosophila melanogaster, GI19921738, Length=386, Percent_Identity=48.7046632124352, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI45553807, Length=453, Percent_Identity=28.0353200883002, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI45553816, Length=453, Percent_Identity=28.0353200883002, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24651721, Length=453, Percent_Identity=28.0353200883002, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI17864154, Length=453, Percent_Identity=28.0353200883002, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24652838, Length=453, Percent_Identity=28.2560706401766, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI17137572, Length=453, Percent_Identity=28.2560706401766, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI19922690, Length=301, Percent_Identity=31.5614617940199, Blast_Score=137, Evalue=9e-33,
Organism=Drosophila melanogaster, GI17137380, Length=341, Percent_Identity=30.2052785923754, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI45550900, Length=442, Percent_Identity=26.9230769230769, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24647046, Length=370, Percent_Identity=27.5675675675676, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24647048, Length=370, Percent_Identity=27.5675675675676, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI78706572, Length=238, Percent_Identity=26.4705882352941, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28572034, Length=231, Percent_Identity=32.4675324675325, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43104; Mature: 42973

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKFDRSKEHANIGTIGHVDHGKTTLTAAIATVLAKNGDSVAQSYDMIDNAPEEKERG
CCCHHHHCCHHCCCCCCEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHCC
ITINTSHIEYQTDKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSAADGPMPQTREHI
EEEECCEEEEECCCCEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHE
LLSRNVGVPALVVFLNKVDMVDDEELLELVEMEVRDLLSEYDFPGDDVPVIAGSALKALE
EEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCHHHHHC
GDAQYEEKILELMEAVDTYIPTPERDSDKPFMMPVEDVFSITGRGTVATGRVERGQIKVG
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCHHHHHHHCCCCEEEECCEECCEEEEC
EEVEIIGLHDTSKTTVTGVEMFRKLLDYAEAGDNIGALLRGVAREDVQRGQVLAAPGSIT
CEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEECCCCCC
PHTEFKAEVYVLSKDEGGRHTPFFSNYRPQFYFRTTDVTGVVHLPEGTEMVMPGDNVEMT
CCCCCEEEEEEEECCCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCCEEEECCCCCEEE
VELIAPIAIEDGTRFSIREGGRTVGSGVVTEIIK
EEEEEEEEECCCCEEEECCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
AKEKFDRSKEHANIGTIGHVDHGKTTLTAAIATVLAKNGDSVAQSYDMIDNAPEEKERG
CCHHHHCCHHCCCCCCEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHCC
ITINTSHIEYQTDKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSAADGPMPQTREHI
EEEECCEEEEECCCCEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHE
LLSRNVGVPALVVFLNKVDMVDDEELLELVEMEVRDLLSEYDFPGDDVPVIAGSALKALE
EEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCHHHHHC
GDAQYEEKILELMEAVDTYIPTPERDSDKPFMMPVEDVFSITGRGTVATGRVERGQIKVG
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCHHHHHHHCCCCEEEECCEECCEEEEC
EEVEIIGLHDTSKTTVTGVEMFRKLLDYAEAGDNIGALLRGVAREDVQRGQVLAAPGSIT
CEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEECCCCCC
PHTEFKAEVYVLSKDEGGRHTPFFSNYRPQFYFRTTDVTGVVHLPEGTEMVMPGDNVEMT
CCCCCEEEEEEEECCCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCCEEEECCCCCEEE
VELIAPIAIEDGTRFSIREGGRTVGSGVVTEIIK
EEEEEEEEECCCCEEEECCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA