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The map label for this gene is hisS

Identifier: 15675899

GI number: 15675899

Start: 1797587

End: 1798867

Strand: Reverse

Name: hisS

Synonym: SPy_2157

Alternate gene names: 15675899

Gene position: 1798867-1797587 (Counterclockwise)

Preceding gene: 15675908

Following gene: 15675898

Centisome position: 97.11

GC content: 41.76

Gene sequence:

>1281_bases
ATGAAACTTCAAAAACCAAAAGGAACCCAGGATATTCTGCCTGGGGATGCTGCGAAATGGCAGTATGTGGAATCTGTTGC
GCGTGATACGTTTAGCCAATATAATTATGGAGAAATTCGTACTCCTATGTTTGAACACTACGAGGTAATCTCGCGGTCTG
TAGGTGATACGACTGATATTGTGACGAAGGAAATGTATGATTTCTATGATAAAGGAGATCGTCACATTACCTTGCGACCA
GAGGGTACAGCTCCAGTGGTGCGTTCTTATGTTGAAAATAAATTGTTTGCGCCAGAGGTCCAAAAACCCGTCAAACTTTA
TTATATCGGTTCCATGTTCCGCTACGAACGTCCTCAAGCTGGTCGTTTACGTGAATTTCACCAGATTGGGGTAGAATGTT
TCGGCGCTGCTAATCCAGCAACAGATGTTGAAACCATTGCGATGGCCTATCACCTCTTTGAAAAATTGGGCATTAAGGAT
GTCACCCTTCATTTAAATAGTTTGGGGAGTCCTGAGAGTCGTGCAGCTTATCGTCAAGCCTTGATTGACTACTTAACACC
AATGCGTGATCAATTATCTAAAGATAGTCAACGTCGCTTGGATGAAAACCCACTGCGCGTGCTTGATTCTAAAGAAAAAG
AAGATAAACTAGCCGTTGAAAAAGCGCCTTCTATTTTGGATTACTTAGATGAGGAAAGTCAGGCGCACTTTGAGGCGGTC
AAAGATATGCTTGAAGCACTTGACATTCCTTATGTGATTGACACCAATATGGTGCGTGGCCTTGATTACTATAGCCACAC
TATTTTTGAATTCATCACGTCTGTGGAAGGTTCTGACTTAACTATCTGTGCGGGTGGACGTTATGATAGTTTGGTAGGTT
ATTTTGGTGGGCCTGAGACTCCAGGATTTGGCTTTGGTCTTGGTTTAGAACGTCTCTTGATGATTATTGAAAAGCAGGGC
ATTACCTTGCCAATTGAGACAGAGATGGATATTTACCTTGCTGTGTTAGGTGATGGTGCTAATAGCAAAGCTTTAGAATT
GGTTCAAGCCATTCGCCGTCAAGGGTTTACAGCTGAACGAGATTACCTTGGCCGTAAGATTAAGGCTCAGTTTAAATCAG
CCGATACCTTTAAAGCAAAATTGGTAATGACTTTAGGAGAAAGTGAAGTTGAAGCAGGCAAAGCTGTGATTAAGAATAAT
CGTAGTCGTCAAGAAGTGGAAGTCAGTTTTGAAGATATGATGACAAACTTTGCGAACATCTCAGAGCAATTATTATCTTA
A

Upstream 100 bases:

>100_bases
GAAAATACTATCAAACTAAAGCAAATGATATATGCTTGTTTAAAATATAAGAAAAAATGGTAGAATGTTTAAAGAGAAAA
CTAATCAAAGAGGTAAACTT

Downstream 100 bases:

>100_bases
TGATTTTATTGTCGTCATAAGCTTGTGTTGATATGATTGTTTAGAAGATAATCACTAAAACAGGTGTAACTTCTCTTTTT
AGAAGGGAGTTGCACCTGTT

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MKLQKPKGTQDILPGDAAKWQYVESVARDTFSQYNYGEIRTPMFEHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRP
EGTAPVVRSYVENKLFAPEVQKPVKLYYIGSMFRYERPQAGRLREFHQIGVECFGAANPATDVETIAMAYHLFEKLGIKD
VTLHLNSLGSPESRAAYRQALIDYLTPMRDQLSKDSQRRLDENPLRVLDSKEKEDKLAVEKAPSILDYLDEESQAHFEAV
KDMLEALDIPYVIDTNMVRGLDYYSHTIFEFITSVEGSDLTICAGGRYDSLVGYFGGPETPGFGFGLGLERLLMIIEKQG
ITLPIETEMDIYLAVLGDGANSKALELVQAIRRQGFTAERDYLGRKIKAQFKSADTFKAKLVMTLGESEVEAGKAVIKNN
RSRQEVEVSFEDMMTNFANISEQLLS

Sequences:

>Translated_426_residues
MKLQKPKGTQDILPGDAAKWQYVESVARDTFSQYNYGEIRTPMFEHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRP
EGTAPVVRSYVENKLFAPEVQKPVKLYYIGSMFRYERPQAGRLREFHQIGVECFGAANPATDVETIAMAYHLFEKLGIKD
VTLHLNSLGSPESRAAYRQALIDYLTPMRDQLSKDSQRRLDENPLRVLDSKEKEDKLAVEKAPSILDYLDEESQAHFEAV
KDMLEALDIPYVIDTNMVRGLDYYSHTIFEFITSVEGSDLTICAGGRYDSLVGYFGGPETPGFGFGLGLERLLMIIEKQG
ITLPIETEMDIYLAVLGDGANSKALELVQAIRRQGFTAERDYLGRKIKAQFKSADTFKAKLVMTLGESEVEAGKAVIKNN
RSRQEVEVSFEDMMTNFANISEQLLS
>Mature_426_residues
MKLQKPKGTQDILPGDAAKWQYVESVARDTFSQYNYGEIRTPMFEHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRP
EGTAPVVRSYVENKLFAPEVQKPVKLYYIGSMFRYERPQAGRLREFHQIGVECFGAANPATDVETIAMAYHLFEKLGIKD
VTLHLNSLGSPESRAAYRQALIDYLTPMRDQLSKDSQRRLDENPLRVLDSKEKEDKLAVEKAPSILDYLDEESQAHFEAV
KDMLEALDIPYVIDTNMVRGLDYYSHTIFEFITSVEGSDLTICAGGRYDSLVGYFGGPETPGFGFGLGLERLLMIIEKQG
ITLPIETEMDIYLAVLGDGANSKALELVQAIRRQGFTAERDYLGRKIKAQFKSADTFKAKLVMTLGESEVEAGKAVIKNN
RSRQEVEVSFEDMMTNFANISEQLLS

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI15029520, Length=473, Percent_Identity=24.9471458773784, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI6996014, Length=469, Percent_Identity=26.226012793177, Blast_Score=117, Evalue=1e-26,
Organism=Escherichia coli, GI1788861, Length=422, Percent_Identity=42.1800947867299, Blast_Score=355, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI71993693, Length=461, Percent_Identity=26.4642082429501, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71993686, Length=461, Percent_Identity=26.4642082429501, Blast_Score=112, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6325290, Length=469, Percent_Identity=24.0938166311301, Blast_Score=105, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24643061, Length=468, Percent_Identity=25.2136752136752, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24643059, Length=487, Percent_Identity=24.435318275154, Blast_Score=118, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_STRP1 (Q99XK9)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_270073.1
- RefSeq:   YP_283177.1
- ProteinModelPortal:   Q99XK9
- SMR:   Q99XK9
- EnsemblBacteria:   EBSTRT00000001039
- EnsemblBacteria:   EBSTRT00000028584
- GeneID:   3571062
- GeneID:   901792
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_2157
- KEGG:   spz:M5005_Spy_1814
- GeneTree:   EBGT00050000027423
- HOGENOM:   HBG616575
- OMA:   VFEWVTT
- ProtClustDB:   PRK00037
- BioCyc:   SPYO160490:SPY2157-MONOMER
- BioCyc:   SPYO293653:M5005_SPY1814-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 48201; Mature: 48201

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLQKPKGTQDILPGDAAKWQYVESVARDTFSQYNYGEIRTPMFEHYEVISRSVGDTTDI
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHH
VTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQKPVKLYYIGSMFRYERPQA
HHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHCCCCCC
GRLREFHQIGVECFGAANPATDVETIAMAYHLFEKLGIKDVTLHLNSLGSPESRAAYRQA
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHH
LIDYLTPMRDQLSKDSQRRLDENPLRVLDSKEKEDKLAVEKAPSILDYLDEESQAHFEAV
HHHHHHHHHHHHCCHHHHHCCCCCHHHHCCCCHHHHHHHHHCHHHHHHHCCHHHHHHHHH
KDMLEALDIPYVIDTNMVRGLDYYSHTIFEFITSVEGSDLTICAGGRYDSLVGYFGGPET
HHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHEECCCCCC
PGFGFGLGLERLLMIIEKQGITLPIETEMDIYLAVLGDGANSKALELVQAIRRQGFTAER
CCCCHHHHHHHHHHHHHHCCCEEEECCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCCHH
DYLGRKIKAQFKSADTFKAKLVMTLGESEVEAGKAVIKNNRSRQEVEVSFEDMMTNFANI
HHHHHHHHHHHCCCCHHHEEEEEEECCHHHHHHHHHHHCCCCHHHHHEEHHHHHHHHHHH
SEQLLS
HHHHCC
>Mature Secondary Structure
MKLQKPKGTQDILPGDAAKWQYVESVARDTFSQYNYGEIRTPMFEHYEVISRSVGDTTDI
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHH
VTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQKPVKLYYIGSMFRYERPQA
HHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHCCCCCC
GRLREFHQIGVECFGAANPATDVETIAMAYHLFEKLGIKDVTLHLNSLGSPESRAAYRQA
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHH
LIDYLTPMRDQLSKDSQRRLDENPLRVLDSKEKEDKLAVEKAPSILDYLDEESQAHFEAV
HHHHHHHHHHHHCCHHHHHCCCCCHHHHCCCCHHHHHHHHHCHHHHHHHCCHHHHHHHHH
KDMLEALDIPYVIDTNMVRGLDYYSHTIFEFITSVEGSDLTICAGGRYDSLVGYFGGPET
HHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHEECCCCCC
PGFGFGLGLERLLMIIEKQGITLPIETEMDIYLAVLGDGANSKALELVQAIRRQGFTAER
CCCCHHHHHHHHHHHHHHCCCEEEECCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCCHH
DYLGRKIKAQFKSADTFKAKLVMTLGESEVEAGKAVIKNNRSRQEVEVSFEDMMTNFANI
HHHHHHHHHHHCCCCHHHEEEEEEECCHHHHHHHHHHHCCCCHHHHHEEHHHHHHHHHHH
SEQLLS
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296