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The map label for this gene is vanSB [H]

Identifier: 15675803

GI number: 15675803

Start: 1689748

End: 1691136

Strand: Reverse

Name: vanSB [H]

Synonym: SPy_2026

Alternate gene names: 15675803

Gene position: 1691136-1689748 (Counterclockwise)

Preceding gene: 15675804

Following gene: 15675802

Centisome position: 91.29

GC content: 38.23

Gene sequence:

>1389_bases
GTGAGACTAATCAAAAAAACCTTTTTGGTCATCAATGGCTTAATTATTGTAGTGGTTACCTCTATTTTGTTAGTGCTTTA
TTTTGCAATGCCAATTTATTACACCAAAGTCAAAGACAAAGAAGTTAAGTGTGAATTTGACCAAACAAGCAAGCAAATCA
AAGGCAAAACGGTAACCGAGATTAGAGATATCCTAACAAAAAAAATCAACAAAGATAATATTTGGTACAGCTTAGTAGAC
AGTGATAACCAACTGCTCTATCCTTCCTTGCAGCTCCTAGACGGCGTCAGTGAAAGCAAAGACAGTCAAAATGTTAATAT
CGTCACCACCTTTGACAACTCTTATTCTAATGTTAAGGTGATGAGTCAAAAAGTAACCCTACGCGATGGAAAAAAAATGA
CATTGCTTGGGCAATCTTCACTTCAACCCGTAACAGATGCTAGCAAAGTTCTCCTTGACCTCTACCCGTCACTATTGATT
TTTTCAGTGACCGTAGGGAGTATTGTCGCCTACTTATATAGTAGGACTTCTAGCCGGCGTATCCTTAGCATGTCACAAAC
CGCCAAAAAAATGGTCAACCTAGAACCAAACTTAACGTGTACCATTCACGGCAAAGATGAAATAGCAATGTTGGCTAGTG
ATATTAACCGTTTGTATGCGAGTCTTTCAACGAGCATCAAATCCTTGCAAAAAGAGTATGAAAAAGCTTCTGATTCAGAA
AGAGAGAAATCTGAGTTTTTACGCATGACATCGCACGAGCTAAAGACACCAATCACTAGTGTTATCGGAATGATTGATGG
AATGCTCTATAATGTTGGTGACTTTGCAGATCGTGACAAATATTTGCGAAAGTGCCGAGACGTCCTTGAAGGGCAAGCCC
AACTGGTTCAATCCATTTTATCTTTATCTAAGATTGAAACCCTAGCTTCTCAAAATCAAGAACTGTTTTCACTAAAAAGC
AGCCTAGAAGAAGAAATGGAAGTCTTTCTCGTCTTATCAGAACTAAAACACCTCAAAGTGACTATCAATCTCGAAGAACA
ATTCGTCAAAGCCAATAAAGTATATCTACTAAAGGCGATTAAAAATATTATTGACAATGCCTTTCACTATACCAAATCAG
GCGGCCAAGTGATGATTCAACTAAAAGACAACCAACTAGTGATTAAAAATGAAGCAGAGACATTATTGACACAACAGCAG
ATGAAACAGTTATTCCAACCGTTTTATCGACCAGATTATAGTCGTAACAGGAAAGACGGTGGTACAGGATTGGGGCTGTT
TATCACCCACCAGATTCTTGATCAGCATCATCTGGCTTATCGCTTTGTTGTTCTTGATCAAAGATGGATGGTATTTACGA
TTGATTTTCCATCCCATCATGACGATTGA

Upstream 100 bases:

>100_bases
CGCGTGTCCTTGACAACCACATCAAGAACCTTCGTAAAAAAATGACGGGTATTCCCCTCAAGACAATTACAGGCATGGGC
TATTTATTGGGAGAGCGTGA

Downstream 100 bases:

>100_bases
TCCATACTAGTAGGACGACAAAAGTCATCAATCATTAACCAAGCCTTGACCTTTACCCATCATTGTTTAGGCAAGTATAA
AAAAGAGGAGAGAAATCATA

Product: putative histidine kinase

Products: NA

Alternate protein names: Vancomycin B-type resistance protein vanSB; Vancomycin histidine protein kinase [H]

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MRLIKKTFLVINGLIIVVVTSILLVLYFAMPIYYTKVKDKEVKCEFDQTSKQIKGKTVTEIRDILTKKINKDNIWYSLVD
SDNQLLYPSLQLLDGVSESKDSQNVNIVTTFDNSYSNVKVMSQKVTLRDGKKMTLLGQSSLQPVTDASKVLLDLYPSLLI
FSVTVGSIVAYLYSRTSSRRILSMSQTAKKMVNLEPNLTCTIHGKDEIAMLASDINRLYASLSTSIKSLQKEYEKASDSE
REKSEFLRMTSHELKTPITSVIGMIDGMLYNVGDFADRDKYLRKCRDVLEGQAQLVQSILSLSKIETLASQNQELFSLKS
SLEEEMEVFLVLSELKHLKVTINLEEQFVKANKVYLLKAIKNIIDNAFHYTKSGGQVMIQLKDNQLVIKNEAETLLTQQQ
MKQLFQPFYRPDYSRNRKDGGTGLGLFITHQILDQHHLAYRFVVLDQRWMVFTIDFPSHHDD

Sequences:

>Translated_462_residues
MRLIKKTFLVINGLIIVVVTSILLVLYFAMPIYYTKVKDKEVKCEFDQTSKQIKGKTVTEIRDILTKKINKDNIWYSLVD
SDNQLLYPSLQLLDGVSESKDSQNVNIVTTFDNSYSNVKVMSQKVTLRDGKKMTLLGQSSLQPVTDASKVLLDLYPSLLI
FSVTVGSIVAYLYSRTSSRRILSMSQTAKKMVNLEPNLTCTIHGKDEIAMLASDINRLYASLSTSIKSLQKEYEKASDSE
REKSEFLRMTSHELKTPITSVIGMIDGMLYNVGDFADRDKYLRKCRDVLEGQAQLVQSILSLSKIETLASQNQELFSLKS
SLEEEMEVFLVLSELKHLKVTINLEEQFVKANKVYLLKAIKNIIDNAFHYTKSGGQVMIQLKDNQLVIKNEAETLLTQQQ
MKQLFQPFYRPDYSRNRKDGGTGLGLFITHQILDQHHLAYRFVVLDQRWMVFTIDFPSHHDD
>Mature_462_residues
MRLIKKTFLVINGLIIVVVTSILLVLYFAMPIYYTKVKDKEVKCEFDQTSKQIKGKTVTEIRDILTKKINKDNIWYSLVD
SDNQLLYPSLQLLDGVSESKDSQNVNIVTTFDNSYSNVKVMSQKVTLRDGKKMTLLGQSSLQPVTDASKVLLDLYPSLLI
FSVTVGSIVAYLYSRTSSRRILSMSQTAKKMVNLEPNLTCTIHGKDEIAMLASDINRLYASLSTSIKSLQKEYEKASDSE
REKSEFLRMTSHELKTPITSVIGMIDGMLYNVGDFADRDKYLRKCRDVLEGQAQLVQSILSLSKIETLASQNQELFSLKS
SLEEEMEVFLVLSELKHLKVTINLEEQFVKANKVYLLKAIKNIIDNAFHYTKSGGQVMIQLKDNQLVIKNEAETLLTQQQ
MKQLFQPFYRPDYSRNRKDGGTGLGLFITHQILDQHHLAYRFVVLDQRWMVFTIDFPSHHDD

Specific function: Member of the two-component regulatory system vanS/vanR. Activates the transcription of vanH, vanA and vanX in response to vancomycin which results in vancomycin resistance. VanS activates vanR by phosphorylation [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=324, Percent_Identity=24.3827160493827, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786783, Length=240, Percent_Identity=25.8333333333333, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1788393, Length=239, Percent_Identity=26.7782426778243, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1786600, Length=211, Percent_Identity=26.0663507109005, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1788279, Length=310, Percent_Identity=25.8064516129032, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788713, Length=221, Percent_Identity=25.3393665158371, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 52837; Mature: 52837

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLIKKTFLVINGLIIVVVTSILLVLYFAMPIYYTKVKDKEVKCEFDQTSKQIKGKTVTE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEECCCHHHHHHCCCHHHH
IRDILTKKINKDNIWYSLVDSDNQLLYPSLQLLDGVSESKDSQNVNIVTTFDNSYSNVKV
HHHHHHHHCCCCCCEEEEECCCCCEECCHHHHHCCCCCCCCCCCCEEEEEECCCCCCEEE
MSQKVTLRDGKKMTLLGQSSLQPVTDASKVLLDLYPSLLIFSVTVGSIVAYLYSRTSSRR
EEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
ILSMSQTAKKMVNLEPNLTCTIHGKDEIAMLASDINRLYASLSTSIKSLQKEYEKASDSE
HHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
REKSEFLRMTSHELKTPITSVIGMIDGMLYNVGDFADRDKYLRKCRDVLEGQAQLVQSIL
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SLSKIETLASQNQELFSLKSSLEEEMEVFLVLSELKHLKVTINLEEQFVKANKVYLLKAI
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEHHHHHHHHHHHHHHHH
KNIIDNAFHYTKSGGQVMIQLKDNQLVIKNEAETLLTQQQMKQLFQPFYRPDYSRNRKDG
HHHHHHHHHEECCCCEEEEEEECCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
GTGLGLFITHQILDQHHLAYRFVVLDQRWMVFTIDFPSHHDD
CCCHHHHHHHHHHHHHHHEEEEEEEECCEEEEEEECCCCCCC
>Mature Secondary Structure
MRLIKKTFLVINGLIIVVVTSILLVLYFAMPIYYTKVKDKEVKCEFDQTSKQIKGKTVTE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEECCCHHHHHHCCCHHHH
IRDILTKKINKDNIWYSLVDSDNQLLYPSLQLLDGVSESKDSQNVNIVTTFDNSYSNVKV
HHHHHHHHCCCCCCEEEEECCCCCEECCHHHHHCCCCCCCCCCCCEEEEEECCCCCCEEE
MSQKVTLRDGKKMTLLGQSSLQPVTDASKVLLDLYPSLLIFSVTVGSIVAYLYSRTSSRR
EEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
ILSMSQTAKKMVNLEPNLTCTIHGKDEIAMLASDINRLYASLSTSIKSLQKEYEKASDSE
HHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
REKSEFLRMTSHELKTPITSVIGMIDGMLYNVGDFADRDKYLRKCRDVLEGQAQLVQSIL
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SLSKIETLASQNQELFSLKSSLEEEMEVFLVLSELKHLKVTINLEEQFVKANKVYLLKAI
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEHHHHHHHHHHHHHHHH
KNIIDNAFHYTKSGGQVMIQLKDNQLVIKNEAETLLTQQQMKQLFQPFYRPDYSRNRKDG
HHHHHHHHHEECCCCEEEEEEECCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
GTGLGLFITHQILDQHHLAYRFVVLDQRWMVFTIDFPSHHDD
CCCHHHHHHHHHHHHHHHEEEEEEEECCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8631706; 12663927 [H]