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The map label for this gene is prfA [H]

Identifier: 15675678

GI number: 15675678

Start: 1541166

End: 1541885

Strand: Direct

Name: prfA [H]

Synonym: SPy_1857

Alternate gene names: 15675678

Gene position: 1541166-1541885 (Clockwise)

Preceding gene: 15675675

Following gene: 15675679

Centisome position: 83.2

GC content: 32.08

Gene sequence:

>720_bases
ATGTTAATTTCTCTTGAAGATACAAATTATACATTGGTAAAAGAATATTTGGAGAAAAGCGTGAATCATCACATTTTGCA
ACGGTATATTGATAATCATAACTTTCCTATCATTGAAAAAAGTTATCACAAGTACTTGACTTTTGAAAGTCTCGAAGAGG
ACTTCACTTATATTTTAAAAGACGGCATTGTGAAACAGAGTGTTCTTTCAAAATATGGAATGGAATTTAATTTGAGATAT
GTTACAGGACTTGAAATTACTTCTGTTCTCAACACTGGTTATTCAAAAGATATGGGAGAGCCTTATAATGTTCGCATCGA
GTCAGAGAAAGCTAGTTTTTACAAGGTTCGCCGCTCAGCATTTTTAAAAGATATAAACGAAGATATTGAACTACAAGGAT
ATGTGAAGGACTTTTACCATAATCGTCTTCAAAAATCCATGAAAAAAATGCAATGTATGTTAACTAACGGCCGTATCGGT
GCCATTTCTACTCAAATATATGACTTAATGACCCTATTTGGCGAAGAACTACCAAATGGCCAAATTTTAATTAATTTTGT
CATTACTAATGAGGAACTCGGTAAATTCTGTGGCATTTCTACCGCTAGCAGCGTCAGCCGTATTCTAAAACAGTTGAAAG
AAAAAAACATCATTCGCATTGATAAACAACATATTATCATTACCAATCTTGATAAATTAAAAGATAATATCGTTTTCTAA

Upstream 100 bases:

>100_bases
ATGAACTCCTCTCTATTTTCATAAACATTTTACAAAAAAAGCAGAATATAATATATTTCAATGGTATAATATAAGTGTTA
CTATCAAAGGGCATTAGCAA

Downstream 100 bases:

>100_bases
GCCACTAGAAAGGAAACTCCTATGCGCTATAATCAATTTTCCTATATTCCAACTAGTCTAGAAAGAGCGGCTGAAGAATT
AAAAGAATTGGGCTTTGATC

Product: putative transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MLISLEDTNYTLVKEYLEKSVNHHILQRYIDNHNFPIIEKSYHKYLTFESLEEDFTYILKDGIVKQSVLSKYGMEFNLRY
VTGLEITSVLNTGYSKDMGEPYNVRIESEKASFYKVRRSAFLKDINEDIELQGYVKDFYHNRLQKSMKKMQCMLTNGRIG
AISTQIYDLMTLFGEELPNGQILINFVITNEELGKFCGISTASSVSRILKQLKEKNIIRIDKQHIIITNLDKLKDNIVF

Sequences:

>Translated_239_residues
MLISLEDTNYTLVKEYLEKSVNHHILQRYIDNHNFPIIEKSYHKYLTFESLEEDFTYILKDGIVKQSVLSKYGMEFNLRY
VTGLEITSVLNTGYSKDMGEPYNVRIESEKASFYKVRRSAFLKDINEDIELQGYVKDFYHNRLQKSMKKMQCMLTNGRIG
AISTQIYDLMTLFGEELPNGQILINFVITNEELGKFCGISTASSVSRILKQLKEKNIIRIDKQHIIITNLDKLKDNIVF
>Mature_239_residues
MLISLEDTNYTLVKEYLEKSVNHHILQRYIDNHNFPIIEKSYHKYLTFESLEEDFTYILKDGIVKQSVLSKYGMEFNLRY
VTGLEITSVLNTGYSKDMGEPYNVRIESEKASFYKVRRSAFLKDINEDIELQGYVKDFYHNRLQKSMKKMQCMLTNGRIG
AISTQIYDLMTLFGEELPNGQILINFVITNEELGKFCGISTASSVSRILKQLKEKNIIRIDKQHIIITNLDKLKDNIVF

Specific function: Positively regulates expression of listeriolysin, of 1- phosphadidylinositol phosphodiesterase (PI-PLC) and other virulence factors [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding [H]

EC number: NA

Molecular weight: Translated: 27924; Mature: 27924

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLISLEDTNYTLVKEYLEKSVNHHILQRYIDNHNFPIIEKSYHKYLTFESLEEDFTYILK
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHH
DGIVKQSVLSKYGMEFNLRYVTGLEITSVLNTGYSKDMGEPYNVRIESEKASFYKVRRSA
HHHHHHHHHHHCCCEEEEEEEECHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHHH
FLKDINEDIELQGYVKDFYHNRLQKSMKKMQCMLTNGRIGAISTQIYDLMTLFGEELPNG
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCC
QILINFVITNEELGKFCGISTASSVSRILKQLKEKNIIRIDKQHIIITNLDKLKDNIVF
EEEEEEEEECHHHHHHHCCCCHHHHHHHHHHHHHCCCEEECCCEEEEECHHHHHHCCCC
>Mature Secondary Structure
MLISLEDTNYTLVKEYLEKSVNHHILQRYIDNHNFPIIEKSYHKYLTFESLEEDFTYILK
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHH
DGIVKQSVLSKYGMEFNLRYVTGLEITSVLNTGYSKDMGEPYNVRIESEKASFYKVRRSA
HHHHHHHHHHHCCCEEEEEEEECHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHHH
FLKDINEDIELQGYVKDFYHNRLQKSMKKMQCMLTNGRIGAISTQIYDLMTLFGEELPNG
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCC
QILINFVITNEELGKFCGISTASSVSRILKQLKEKNIIRIDKQHIIITNLDKLKDNIVF
EEEEEEEEECHHHHHHHCCCCHHHHHHHHHHHHHCCCEEECCCEEEEECHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1662763; 2122460; 9045810; 11679669 [H]