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The map label for this gene is gatA

Identifier: 15675615

GI number: 15675615

Start: 1465591

End: 1467057

Strand: Reverse

Name: gatA

Synonym: SPy_1771

Alternate gene names: 15675615

Gene position: 1467057-1465591 (Counterclockwise)

Preceding gene: 15675616

Following gene: 15675614

Centisome position: 79.2

GC content: 43.01

Gene sequence:

>1467_bases
ATGTCTTTTAATCATAAAACCATTGAAGAGTTGCATGACCTCCTTGTAGCTAAGGAGATTTCAGCAACAGAATTGACCCA
AGCAACACTTGAAGACATCAAGTCTCGTGAGGAGGCTGTCGGATCGTTTATCACTGTTTCAGAAGAAGTTGCTCTTAAAC
AAGCTGCGGCAATTGATGCCAAAGGTATTGATGCTGATAACCTCATGTCAGGTATTCCGTTAGCTGTTAAGGATAATATT
TCAACCAAGGAAATCCTAACCACAGCCGCTTCAAAAATGTTGTATAACTACGAGCCGATTTTTAATGCCACATCAGTAGC
AAATGCCTACGCTAAAGACATGATTGTTATTGGAAAAACCAACATGGACGAATTTGCCATGGGTGGTTCAACAGAAACCT
CTTATTTCAAGAAAACGAAAAATGCTTGGGACCACACAAAAGTACCTGGAGGCTCATCAGGTGGTTCGGCAACAGCAGTC
GCTTCAGGACAAGTCCGTTTATCTCTTGGTTCAGACACTGGTGGTTCTATTCGGCAGCCAGCTGCATTTAATAGTGTTGT
CGGTTTGAAACCAACCTATGGCACTGTTTCTCGCTATGGTCTCATTGCTTTTGGAAGTTCACTTGATCAGATTGGACCTT
TTGCTCCGACAGTTAAAGAAAACGCCCAATTATTAAATGTGATTGCAAGTAGCGATGTCAAGGATGCCACTTCAGCGCCT
GTCCGCATTGCAGACTACACATCTAAGATTGGTCGTGACATTAAAGGCATGAAGATTGCCCTTCCTAAAGAGTATCTTGG
CGAAGGGATTGATCCTGAGATTAAGGAAACGGTTCTTGCGTCAGTTAAACAATTTGAAGCCTTAGGAGCTACTGTTGAGG
AAGTCAGCCTTCCACATAGCAAGTACGGTGTGGCTGTTTATTATATTATCGCTTCGTCAGAAGCGTCTTCAAACTTACAA
CGTTTTGATGGCATTCGCTATGGTTTCCGTGCTGACGATGCTAAAAACTTGGATGAAATCTATGTTAACACTCGTAGCCA
AGGCTTTGGTGATGAGGTGAAACGTCGTATCATGCTTGGAACCTTTAGTTTGTCATCGGGTTATTATGACGCTTATTTCA
AAAAGGCCGGTCAAGTGCGCACTTTGATTATTCAAGATTTTGACAAGGTTTTTGCGGATTATGATCTTATTCTAGGCCCA
ACAACACCGACGGTTGCTTTTGGCTTAGACACACTCAACCACGATCCTGTTGCCATGTATTTGGCCGACTTATTGACCAT
TCCGGTTAACTTGGCTGGTCTGCCAGGTATTTCTATTCCAGCAGGATTCGTCGATGGCTTGCCAGTAGGGCTTCAATTAA
TTGGTCCGAAATACGCTGAAGAAACCATCTATCAAGCAGCAGCAGCCTTTGAAGCGGTGACAGATTATCACAAGCAACAA
CCGATTATTTTTGGAGGTGACAAGTAA

Upstream 100 bases:

>100_bases
AAGAAGGAACAGACCGTGCCTTGCTCTTTAAAAATGTTCCTGAAAAAGAAAATCACTTTATCAAGGTACCTGCTATTTTG
GATGACGGAGGAGATGCCTA

Downstream 100 bases:

>100_bases
TGAATTTTGAAACCATTATTGGGCTTGAAGTCCATGTGGAATTAAATACCAATTCCAAAATTTTCTCACCTTCGTCAGCT
CACTTTGGTGAGGACCCAAA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MSFNHKTIEELHDLLVAKEISATELTQATLEDIKSREEAVGSFITVSEEVALKQAAAIDAKGIDADNLMSGIPLAVKDNI
STKEILTTAASKMLYNYEPIFNATSVANAYAKDMIVIGKTNMDEFAMGGSTETSYFKKTKNAWDHTKVPGGSSGGSATAV
ASGQVRLSLGSDTGGSIRQPAAFNSVVGLKPTYGTVSRYGLIAFGSSLDQIGPFAPTVKENAQLLNVIASSDVKDATSAP
VRIADYTSKIGRDIKGMKIALPKEYLGEGIDPEIKETVLASVKQFEALGATVEEVSLPHSKYGVAVYYIIASSEASSNLQ
RFDGIRYGFRADDAKNLDEIYVNTRSQGFGDEVKRRIMLGTFSLSSGYYDAYFKKAGQVRTLIIQDFDKVFADYDLILGP
TTPTVAFGLDTLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFVDGLPVGLQLIGPKYAEETIYQAAAAFEAVTDYHKQQ
PIIFGGDK

Sequences:

>Translated_488_residues
MSFNHKTIEELHDLLVAKEISATELTQATLEDIKSREEAVGSFITVSEEVALKQAAAIDAKGIDADNLMSGIPLAVKDNI
STKEILTTAASKMLYNYEPIFNATSVANAYAKDMIVIGKTNMDEFAMGGSTETSYFKKTKNAWDHTKVPGGSSGGSATAV
ASGQVRLSLGSDTGGSIRQPAAFNSVVGLKPTYGTVSRYGLIAFGSSLDQIGPFAPTVKENAQLLNVIASSDVKDATSAP
VRIADYTSKIGRDIKGMKIALPKEYLGEGIDPEIKETVLASVKQFEALGATVEEVSLPHSKYGVAVYYIIASSEASSNLQ
RFDGIRYGFRADDAKNLDEIYVNTRSQGFGDEVKRRIMLGTFSLSSGYYDAYFKKAGQVRTLIIQDFDKVFADYDLILGP
TTPTVAFGLDTLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFVDGLPVGLQLIGPKYAEETIYQAAAAFEAVTDYHKQQ
PIIFGGDK
>Mature_487_residues
SFNHKTIEELHDLLVAKEISATELTQATLEDIKSREEAVGSFITVSEEVALKQAAAIDAKGIDADNLMSGIPLAVKDNIS
TKEILTTAASKMLYNYEPIFNATSVANAYAKDMIVIGKTNMDEFAMGGSTETSYFKKTKNAWDHTKVPGGSSGGSATAVA
SGQVRLSLGSDTGGSIRQPAAFNSVVGLKPTYGTVSRYGLIAFGSSLDQIGPFAPTVKENAQLLNVIASSDVKDATSAPV
RIADYTSKIGRDIKGMKIALPKEYLGEGIDPEIKETVLASVKQFEALGATVEEVSLPHSKYGVAVYYIIASSEASSNLQR
FDGIRYGFRADDAKNLDEIYVNTRSQGFGDEVKRRIMLGTFSLSSGYYDAYFKKAGQVRTLIIQDFDKVFADYDLILGPT
TPTVAFGLDTLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFVDGLPVGLQLIGPKYAEETIYQAAAAFEAVTDYHKQQP
IIFGGDK

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=506, Percent_Identity=37.1541501976285, Blast_Score=298, Evalue=8e-81,
Organism=Homo sapiens, GI195972892, Length=487, Percent_Identity=23.4086242299795, Blast_Score=108, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17543272, Length=444, Percent_Identity=36.4864864864865, Blast_Score=256, Evalue=2e-68,
Organism=Caenorhabditis elegans, GI17537465, Length=478, Percent_Identity=22.1757322175732, Blast_Score=95, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17538252, Length=306, Percent_Identity=26.1437908496732, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI71990152, Length=188, Percent_Identity=29.2553191489362, Blast_Score=72, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17556264, Length=164, Percent_Identity=30.4878048780488, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=415, Percent_Identity=34.2168674698795, Blast_Score=222, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6319685, Length=430, Percent_Identity=29.5348837209302, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6320448, Length=259, Percent_Identity=27.4131274131274, Blast_Score=67, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24648113, Length=490, Percent_Identity=38.5714285714286, Blast_Score=292, Evalue=4e-79,
Organism=Drosophila melanogaster, GI24652985, Length=466, Percent_Identity=24.4635193133047, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI19922090, Length=466, Percent_Identity=24.4635193133047, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24652981, Length=466, Percent_Identity=24.4635193133047, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24652983, Length=466, Percent_Identity=24.4635193133047, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24644968, Length=510, Percent_Identity=23.5294117647059, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI45550774, Length=208, Percent_Identity=30.7692307692308, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24648435, Length=208, Percent_Identity=30.7692307692308, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24648437, Length=208, Percent_Identity=30.7692307692308, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI161078093, Length=500, Percent_Identity=22, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24648441, Length=138, Percent_Identity=34.7826086956522, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24648439, Length=138, Percent_Identity=34.7826086956522, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI21356731, Length=331, Percent_Identity=24.773413897281, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_STRP1 (Q99YC0)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_269789.1
- RefSeq:   YP_282870.1
- ProteinModelPortal:   Q99YC0
- SMR:   Q99YC0
- EnsemblBacteria:   EBSTRT00000000466
- EnsemblBacteria:   EBSTRT00000027591
- GeneID:   3571391
- GeneID:   902006
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_1771
- KEGG:   spz:M5005_Spy_1507
- GeneTree:   EBGT00050000026783
- HOGENOM:   HBG481888
- OMA:   RWGLIAF
- ProtClustDB:   PRK00012
- BioCyc:   SPYO160490:SPY1771-MONOMER
- BioCyc:   SPYO293653:M5005_SPY1507-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 52321; Mature: 52190

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 77-77 ACT_SITE 152-152 ACT_SITE 176-176

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFNHKTIEELHDLLVAKEISATELTQATLEDIKSREEAVGSFITVSEEVALKQAAAIDA
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHCCC
KGIDADNLMSGIPLAVKDNISTKEILTTAASKMLYNYEPIFNATSVANAYAKDMIVIGKT
CCCCHHHHHCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCEEEEECC
NMDEFAMGGSTETSYFKKTKNAWDHTKVPGGSSGGSATAVASGQVRLSLGSDTGGSIRQP
CHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCEEEEEECCCCCCCCCCC
AAFNSVVGLKPTYGTVSRYGLIAFGSSLDQIGPFAPTVKENAQLLNVIASSDVKDATSAP
CHHCCCCCCCCCCCCHHHCCCEEECCCHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
VRIADYTSKIGRDIKGMKIALPKEYLGEGIDPEIKETVLASVKQFEALGATVEEVSLPHS
EEEHHHHHHHCCCCCCCEEECCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCC
KYGVAVYYIIASSEASSNLQRFDGIRYGFRADDAKNLDEIYVNTRSQGFGDEVKRRIMLG
CCCEEEEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCHHEEEECCCCCCCHHHHHHHEEE
TFSLSSGYYDAYFKKAGQVRTLIIQDFDKVFADYDLILGPTTPTVAFGLDTLNHDPVAMY
EEECCCCCHHHHHHCCCCEEEHHHHHHHHHHCCCCEEECCCCCCEEEECCCCCCCHHHHH
LADLLTIPVNLAGLPGISIPAGFVDGLPVGLQLIGPKYAEETIYQAAAAFEAVTDYHKQQ
HHHHHHHCCCCCCCCCCCCCCHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCC
PIIFGGDK
CEEECCCC
>Mature Secondary Structure 
SFNHKTIEELHDLLVAKEISATELTQATLEDIKSREEAVGSFITVSEEVALKQAAAIDA
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHCCC
KGIDADNLMSGIPLAVKDNISTKEILTTAASKMLYNYEPIFNATSVANAYAKDMIVIGKT
CCCCHHHHHCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCEEEEECC
NMDEFAMGGSTETSYFKKTKNAWDHTKVPGGSSGGSATAVASGQVRLSLGSDTGGSIRQP
CHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCEEEEEECCCCCCCCCCC
AAFNSVVGLKPTYGTVSRYGLIAFGSSLDQIGPFAPTVKENAQLLNVIASSDVKDATSAP
CHHCCCCCCCCCCCCHHHCCCEEECCCHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
VRIADYTSKIGRDIKGMKIALPKEYLGEGIDPEIKETVLASVKQFEALGATVEEVSLPHS
EEEHHHHHHHCCCCCCCEEECCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCC
KYGVAVYYIIASSEASSNLQRFDGIRYGFRADDAKNLDEIYVNTRSQGFGDEVKRRIMLG
CCCEEEEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCHHEEEECCCCCCCHHHHHHHEEE
TFSLSSGYYDAYFKKAGQVRTLIIQDFDKVFADYDLILGPTTPTVAFGLDTLNHDPVAMY
EEECCCCCHHHHHHCCCCEEEHHHHHHHHHHCCCCEEECCCCCCEEEECCCCCCCHHHHH
LADLLTIPVNLAGLPGISIPAGFVDGLPVGLQLIGPKYAEETIYQAAAAFEAVTDYHKQQ
HHHHHHHCCCCCCCCCCCCCCHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCC
PIIFGGDK
CEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296