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The map label for this gene is gatB [H]

Identifier: 15675614

GI number: 15675614

Start: 1464152

End: 1465591

Strand: Reverse

Name: gatB [H]

Synonym: SPy_1770

Alternate gene names: 15675614

Gene position: 1465591-1464152 (Counterclockwise)

Preceding gene: 15675615

Following gene: 15675613

Centisome position: 79.12

GC content: 43.33

Gene sequence:

>1440_bases
ATGAATTTTGAAACCATTATTGGGCTTGAAGTCCATGTGGAATTAAATACCAATTCCAAAATTTTCTCACCTTCGTCAGC
TCACTTTGGTGAGGACCCAAATGCCAACACGAATGTGATTGATTGGTCTTTCCCAGGGGTCTTACCAGTCATGAACAAAG
GGGTGATTGATGCGGGCATAAAAGCAGCGCTTGCCCTTAATATGGACATCCATAAAGAGATGCACTTTGACCGCAAAAAT
TATTTCTACCCAGATAATCCTAAAGCTTATCAAATTTCACAGTTTGATGAGCCGATTGGCTACAACGGCTGGATTGATAT
CAAACTAGAAGACGGTTCAACCAAGAAAATTCGTATTGAACGCGCACACTTGGAAGAAGATGCTGGTAAAAACACTCATG
GCACAGATGGTTATTCTTATGTGGACCTTAACCGCCAAGGAGTTCCTTTGATTGAGATTGTGTCAGAAGCCGACATGCGT
TCTCCTGAAGAAGCCTATGCTTATTTGACAGCTCTGAAAGAAATCATCCAGTATACGGGTATTTCTGATGTCAAGATGGA
AGAAGGCTCCATGCGTGTGGATGCCAATATTTCTCTTCGTCCTTACGGGCAAGAACAATTTGGAACAAAGACCGAATTGA
AAAACTTGAACTCCTTCTCAAATGTCCGTAAAGGGCTTGAATTTGAGGTGGAACGTCAAGCCAAACTCTTGCGTTCAGGT
GGTGTGATACGCCAAGAAACGCGCCGCTATGACGAAGCGAATAAGGGGACTATTTTGATGCGGGTCAAAGAAGGGGCAGC
GGACTATCGTTATTTCCCAGAACCAGACCTGCCATTATATGAGATTGATGATGCTTGGATTGACGAGATGCGTGCTCAAT
TACCTCAATTCCCAGCTCAGCGTCGTGCTAAATACGAAGAGGAACTTGGCCTCTCAGCTTATGATGCTAGCCAGCTAACA
GCTACCAAAGTCTTGTCAGATTTCTTTGAAACTGCAGTAAGTCTTGGTGGCGATGCCAAGCAAGTCTCTAACTGGTTGCA
AGGAGAAGTTGCCCAATTCTTAAATGCAGAAGGCAAAACCATTGAAGAAATCGCTCTGACACCAGAAAATTTGGTGGAGA
TGATTGCTATCATTGCTGATGGTACAATCTCTTCTAAAATGGCTAAGAAGGTCTTTGTGCATTTGGCTAAAAACGGTGGC
TCAGCACGTGCCTACGTCGAAAAGGCAGGACTTGTTCAGATTTCTGATCCCGCTGTTTTGGTACCAATTATTCACCAAGT
CTTTGCTGATAATGAAGCTGCAGTAGCTGATTTCAAATCAGGCAAACGTAATGCTGATAAGGCTTTCACAGGCTTCTTGA
TGAAGGCAACCAAAGGTCAAGCTAATCCACAAGTCGCTCAACAATTATTGGCACAAGAATTGCAAAAATTGCGAGATTGA

Upstream 100 bases:

>100_bases
CGAAATACGCTGAAGAAACCATCTATCAAGCAGCAGCAGCCTTTGAAGCGGTGACAGATTATCACAAGCAACAACCGATT
ATTTTTGGAGGTGACAAGTA

Downstream 100 bases:

>100_bases
TAGTTGCTGATAGCAATAAGTTAAAGTATTTTGTCTCTGAAAAAAAGGCGGTCGGTCTTTCACAGGCTGCCTTTTTGTAT
GTCATGTTAGTGACCGCCTA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MNFETIIGLEVHVELNTNSKIFSPSSAHFGEDPNANTNVIDWSFPGVLPVMNKGVIDAGIKAALALNMDIHKEMHFDRKN
YFYPDNPKAYQISQFDEPIGYNGWIDIKLEDGSTKKIRIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
SPEEAYAYLTALKEIIQYTGISDVKMEEGSMRVDANISLRPYGQEQFGTKTELKNLNSFSNVRKGLEFEVERQAKLLRSG
GVIRQETRRYDEANKGTILMRVKEGAADYRYFPEPDLPLYEIDDAWIDEMRAQLPQFPAQRRAKYEEELGLSAYDASQLT
ATKVLSDFFETAVSLGGDAKQVSNWLQGEVAQFLNAEGKTIEEIALTPENLVEMIAIIADGTISSKMAKKVFVHLAKNGG
SARAYVEKAGLVQISDPAVLVPIIHQVFADNEAAVADFKSGKRNADKAFTGFLMKATKGQANPQVAQQLLAQELQKLRD

Sequences:

>Translated_479_residues
MNFETIIGLEVHVELNTNSKIFSPSSAHFGEDPNANTNVIDWSFPGVLPVMNKGVIDAGIKAALALNMDIHKEMHFDRKN
YFYPDNPKAYQISQFDEPIGYNGWIDIKLEDGSTKKIRIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
SPEEAYAYLTALKEIIQYTGISDVKMEEGSMRVDANISLRPYGQEQFGTKTELKNLNSFSNVRKGLEFEVERQAKLLRSG
GVIRQETRRYDEANKGTILMRVKEGAADYRYFPEPDLPLYEIDDAWIDEMRAQLPQFPAQRRAKYEEELGLSAYDASQLT
ATKVLSDFFETAVSLGGDAKQVSNWLQGEVAQFLNAEGKTIEEIALTPENLVEMIAIIADGTISSKMAKKVFVHLAKNGG
SARAYVEKAGLVQISDPAVLVPIIHQVFADNEAAVADFKSGKRNADKAFTGFLMKATKGQANPQVAQQLLAQELQKLRD
>Mature_479_residues
MNFETIIGLEVHVELNTNSKIFSPSSAHFGEDPNANTNVIDWSFPGVLPVMNKGVIDAGIKAALALNMDIHKEMHFDRKN
YFYPDNPKAYQISQFDEPIGYNGWIDIKLEDGSTKKIRIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
SPEEAYAYLTALKEIIQYTGISDVKMEEGSMRVDANISLRPYGQEQFGTKTELKNLNSFSNVRKGLEFEVERQAKLLRSG
GVIRQETRRYDEANKGTILMRVKEGAADYRYFPEPDLPLYEIDDAWIDEMRAQLPQFPAQRRAKYEEELGLSAYDASQLT
ATKVLSDFFETAVSLGGDAKQVSNWLQGEVAQFLNAEGKTIEEIALTPENLVEMIAIIADGTISSKMAKKVFVHLAKNGG
SARAYVEKAGLVQISDPAVLVPIIHQVFADNEAAVADFKSGKRNADKAFTGFLMKATKGQANPQVAQQLLAQELQKLRD

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=494, Percent_Identity=35.0202429149798, Blast_Score=285, Evalue=9e-77,
Organism=Caenorhabditis elegans, GI115534094, Length=346, Percent_Identity=34.6820809248555, Blast_Score=206, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6319391, Length=506, Percent_Identity=29.8418972332016, Blast_Score=197, Evalue=2e-51,
Organism=Drosophila melanogaster, GI45550808, Length=496, Percent_Identity=31.6532258064516, Blast_Score=250, Evalue=1e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 53430; Mature: 53430

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFETIIGLEVHVELNTNSKIFSPSSAHFGEDPNANTNVIDWSFPGVLPVMNKGVIDAGI
CCCCEEEEEEEEEEECCCCEEECCCCCCCCCCCCCCCCEEEECCCCHHHHHCCCCHHHCC
KAALALNMDIHKEMHFDRKNYFYPDNPKAYQISQFDEPIGYNGWIDIKLEDGSTKKIRIE
CEEEEECCHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCEEEEEECCCCCCEEEEE
RAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMRSPEEAYAYLTALKEIIQYTG
HHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
ISDVKMEEGSMRVDANISLRPYGQEQFGTKTELKNLNSFSNVRKGLEFEVERQAKLLRSG
CCCEEECCCCEEEECCEEECCCCHHHCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHC
GVIRQETRRYDEANKGTILMRVKEGAADYRYFPEPDLPLYEIDDAWIDEMRAQLPQFPAQ
CCHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCEEECCHHHHHHHHHHCCCCCHH
RRAKYEEELGLSAYDASQLTATKVLSDFFETAVSLGGDAKQVSNWLQGEVAQFLNAEGKT
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCC
IEEIALTPENLVEMIAIIADGTISSKMAKKVFVHLAKNGGSARAYVEKAGLVQISDPAVL
HHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEHHCCEEEECCCEEH
VPIIHQVFADNEAAVADFKSGKRNADKAFTGFLMKATKGQANPQVAQQLLAQELQKLRD
HHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNFETIIGLEVHVELNTNSKIFSPSSAHFGEDPNANTNVIDWSFPGVLPVMNKGVIDAGI
CCCCEEEEEEEEEEECCCCEEECCCCCCCCCCCCCCCCEEEECCCCHHHHHCCCCHHHCC
KAALALNMDIHKEMHFDRKNYFYPDNPKAYQISQFDEPIGYNGWIDIKLEDGSTKKIRIE
CEEEEECCHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCEEEEEECCCCCCEEEEE
RAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMRSPEEAYAYLTALKEIIQYTG
HHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
ISDVKMEEGSMRVDANISLRPYGQEQFGTKTELKNLNSFSNVRKGLEFEVERQAKLLRSG
CCCEEECCCCEEEECCEEECCCCHHHCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHC
GVIRQETRRYDEANKGTILMRVKEGAADYRYFPEPDLPLYEIDDAWIDEMRAQLPQFPAQ
CCHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCEEECCHHHHHHHHHHCCCCCHH
RRAKYEEELGLSAYDASQLTATKVLSDFFETAVSLGGDAKQVSNWLQGEVAQFLNAEGKT
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCC
IEEIALTPENLVEMIAIIADGTISSKMAKKVFVHLAKNGGSARAYVEKAGLVQISDPAVL
HHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEHHCCEEEECCCEEH
VPIIHQVFADNEAAVADFKSGKRNADKAFTGFLMKATKGQANPQVAQQLLAQELQKLRD
HHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA