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The map label for this gene is trmB

Identifier: 15675576

GI number: 15675576

Start: 1430395

End: 1431030

Strand: Reverse

Name: trmB

Synonym: SPy_1726

Alternate gene names: 15675576

Gene position: 1431030-1430395 (Counterclockwise)

Preceding gene: 15675577

Following gene: 15675575

Centisome position: 77.25

GC content: 40.09

Gene sequence:

>636_bases
ATGCGAGTTAGAAAACGAAAAGGTGCTGAGGAGCACTTAGCTAATAACCCACACTATGTGATATTAAATCCAGAAGATGC
CAAGGGCCGTTGGCATGACGTTTTTGGAAATGACCGTCCTATTCATATCGAAGTTGGTTCAGGAAAGGGCGGATTTATCA
CAGGGATGGCCTTGAAAAATCCTGATATTAACTACATTGGCATTGATATTCAATTATCTGTCCTAAGCTACGCCTTGGAT
AAGGTATTAGCTTCAGAAGTACCCAATGTCAAACTTCTGCGAGTGGATGGATCAAGTTTAACCAACTATTTTGAAGATGG
TGAGGTGGATATGATGTACCTCAACTTTTCAGATCCTTGGCCAAAAACCAAGCATGAAAAACGTCGTTTGACCTACAAGG
ATTTCTTAGACACTTACAAGCGTATCTTGCCAGAACACGGTGAAATTCATTTTAAAACCGATAATCGGGGCTTGTTTGAG
TACAGTTTAGCTAGTTTTTCTCAATACGGCATGACCTTGAGACAGATCTGGCTTGACTTACACGCTAGTAACTATGAAGG
AAATGTCATGACAGAATATGAAGAGAAGTTCTCTAATAAAGGTCAAGTCATTTACCGAGTTGAGGCAAATTTCTAG

Upstream 100 bases:

>100_bases
TGACACAAATTCTCAAGTGTTTTGACAAGCGTGACATGGAGCATGTGAATCAGGAGATTTATGCCCTCAGAAAATTTAGA
GAAATATTTAGAAAGAAATA

Downstream 100 bases:

>100_bases
TAGTATTTTGTTTCAAAATATGATAACATAAAGGAGTTGATACAGTATTAACTTCTTTAGTAATGGAGAGGTCGCATAGT
GGCTTAGTGCGCACGCTTGG

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MRVRKRKGAEEHLANNPHYVILNPEDAKGRWHDVFGNDRPIHIEVGSGKGGFITGMALKNPDINYIGIDIQLSVLSYALD
KVLASEVPNVKLLRVDGSSLTNYFEDGEVDMMYLNFSDPWPKTKHEKRRLTYKDFLDTYKRILPEHGEIHFKTDNRGLFE
YSLASFSQYGMTLRQIWLDLHASNYEGNVMTEYEEKFSNKGQVIYRVEANF

Sequences:

>Translated_211_residues
MRVRKRKGAEEHLANNPHYVILNPEDAKGRWHDVFGNDRPIHIEVGSGKGGFITGMALKNPDINYIGIDIQLSVLSYALD
KVLASEVPNVKLLRVDGSSLTNYFEDGEVDMMYLNFSDPWPKTKHEKRRLTYKDFLDTYKRILPEHGEIHFKTDNRGLFE
YSLASFSQYGMTLRQIWLDLHASNYEGNVMTEYEEKFSNKGQVIYRVEANF
>Mature_211_residues
MRVRKRKGAEEHLANNPHYVILNPEDAKGRWHDVFGNDRPIHIEVGSGKGGFITGMALKNPDINYIGIDIQLSVLSYALD
KVLASEVPNVKLLRVDGSSLTNYFEDGEVDMMYLNFSDPWPKTKHEKRRLTYKDFLDTYKRILPEHGEIHFKTDNRGLFE
YSLASFSQYGMTLRQIWLDLHASNYEGNVMTEYEEKFSNKGQVIYRVEANF

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=135, Percent_Identity=28.1481481481481, Blast_Score=67, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17536689, Length=140, Percent_Identity=30, Blast_Score=63, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_STRP1 (P67504)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_269750.1
- RefSeq:   YP_282831.1
- ProteinModelPortal:   P67504
- SMR:   P67504
- EnsemblBacteria:   EBSTRT00000000700
- EnsemblBacteria:   EBSTRT00000028393
- GeneID:   3571429
- GeneID:   901962
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_1726
- KEGG:   spz:M5005_Spy_1468
- GeneTree:   EBGT00050000028276
- HOGENOM:   HBG304014
- OMA:   LRNKPWA
- ProtClustDB:   PRK00121
- BioCyc:   SPYO160490:SPY1726-MONOMER
- BioCyc:   SPYO293653:M5005_SPY1468-MONOMER
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 24455; Mature: 24455

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: BINDING 44-44 BINDING 69-69 BINDING 96-96 BINDING 118-118 BINDING 122-122 BINDING 154-154

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVRKRKGAEEHLANNPHYVILNPEDAKGRWHDVFGNDRPIHIEVGSGKGGFITGMALKN
CCCCCCCCCHHHHCCCCCEEEECCCCCCCCEEECCCCCCEEEEEECCCCCCEEEEEEECC
PDINYIGIDIQLSVLSYALDKVLASEVPNVKLLRVDGSSLTNYFEDGEVDMMYLNFSDPW
CCCCEEEEEEEHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHCCCCCEEEEEEECCCCC
PKTKHEKRRLTYKDFLDTYKRILPEHGEIHFKTDNRGLFEYSLASFSQYGMTLRQIWLDL
CCCCHHHHCCCHHHHHHHHHHHCCCCCEEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHH
HASNYEGNVMTEYEEKFSNKGQVIYRVEANF
CCCCCCCCEEEHHHHHHCCCCCEEEEEECCC
>Mature Secondary Structure
MRVRKRKGAEEHLANNPHYVILNPEDAKGRWHDVFGNDRPIHIEVGSGKGGFITGMALKN
CCCCCCCCCHHHHCCCCCEEEECCCCCCCCEEECCCCCCEEEEEECCCCCCEEEEEEECC
PDINYIGIDIQLSVLSYALDKVLASEVPNVKLLRVDGSSLTNYFEDGEVDMMYLNFSDPW
CCCCEEEEEEEHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHCCCCCEEEEEEECCCCC
PKTKHEKRRLTYKDFLDTYKRILPEHGEIHFKTDNRGLFEYSLASFSQYGMTLRQIWLDL
CCCCHHHHCCCHHHHHHHHHHHCCCCCEEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHH
HASNYEGNVMTEYEEKFSNKGQVIYRVEANF
CCCCCCCCEEEHHHHHHCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11296296