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The map label for this gene is glyS

Identifier: 15675548

GI number: 15675548

Start: 1401260

End: 1403299

Strand: Reverse

Name: glyS

Synonym: SPy_1688

Alternate gene names: 15675548

Gene position: 1403299-1401260 (Counterclockwise)

Preceding gene: 15675549

Following gene: 15675547

Centisome position: 75.75

GC content: 43.68

Gene sequence:

>2040_bases
ATGAGTAAAAATCTACTAATCGAACTTGGTCTGGAAGAACTTCCAGCCTACGTGGTCACACCAAGTGAAAAACAACTGGG
AGAGCGCTTGGCGACTTTCTTAACAGAAAATCGTCTCTCTTTTGAAGATATACAAACCTTTTCAACTCCACGCCGTTTGG
CAGTGCGTGTGTCTGGATTAGCTGATCAGCAAACGGATTTGACTGAGGACTTTAAAGGGCCTGCTAAGAAAATTGCCCTT
GATGCTGATGGGAATTTCTCAAAAGCTGCTCAAGGATTTGTTCGTGGCAAAGGCTTGACCACAGATGCTATCGAATTCCG
CGAAGTCAAAGGAGAAGAATACGTTTATGTGACCAAACACGAAGCTGGAAAACCTGCTAAGGAAGTGCTTCTAGGCGTTA
CTGAAGTGTTATCAGCAATGACCTTCCCAGTCAGCATGCACTGGGCTAACAATAGTTTTGAATACATTCGTCCTGTTCAC
ACCTTGACAGTTCTGTTGAATGATGAGGCCTTGGAACTTGACTTCTTGGATATTCATTCTGGACGCGTCAGCCGTGGTCA
CCGTTTTTTAGGTACTGAAACCACTATCACAAGTGCTGATTCTTATGAGGCTGATTTACGCAGCCAGTTTGTTATCGCTG
ATGCTAAAGAGCGTCAAGAGATGATTGTGGAGCAAATCAAGACGCTTGAAGTGGAGCAGGGTGTGCAAGTTGACATTGAC
GAAGACTTGCTTAATGAAGTTTTGAACTTGGTCGAGTTTCCAACAGCTTTCATGGGAAGTTTTGAGGCTAAATACCTTGA
TGTCCCAGAAGAAGTATTGGTAACATCAATGAAAAACCACCAACGTTATTTTGTAGTACGTGATCAGGCTGGTCACTTGA
TGCCAAACTTTGTATCAGTTCGTAATGGGAATGATCAAGCCATTGAAAATGTTATTAAAGGGAACGAAAAAGTTTTGGTA
GCTCGCTTAGAAGATGGTGAATTCTTCTGGCGTGAGGATCAGAAATTGCAAATTGCTGATTTGGTTGCCAAATTGACCAA
TGTGACCTTCCATGAAAAAATCGGCTCTCTTGCTGAACACATGGATCGTACGCGTGTCATTGCCGCATCACTTGCTAAGG
AAGCCAACCTATCTGCTGAGGAAGTCACAGCTGTTGACCGTGCTGCCCAAATTTACAAATTTGACCTGTTAACAGGTATG
GTGGGTGAATTTGATGAGCTGCAAGGGATTATGGGTGAAAAATATGCCCTTCTAGCTGGTGAAGATGCTGCTGTGGCAAC
AGCAATCCGTGAACACTACTTACCTGATGCTGCAGGAGGTGCGCTACCTGAAACCAAAGTTGGCGCTGTCCTGGCCTTGG
CAGCTAAATTGGATACTCTCTTGTCATTCTTTTCTGTTGGCTTGATTCCATCTGGCTCTAATGATCCTTATGCTCTACGT
CGTGCAACCCAAGGGATTGTCCGTATTCTTGATCATTTTGGATGGCGCATCCCAATGGATAAGCTGGTAGATAGCTTATA
TGACTTGTCCTTTGATAGTTTGACCTATGCCAATAAGGCGGATGTGATGAACTTTATCCGTGCTCGTGTGGATAAGATGA
TGGGCAAAGCCGCACCAAAAGACATTCGGGAAGCTATTCTAGCAAGTTCAACCTTTGTGGTTCCAGAAATGTTGGCCGCA
GCTGAAGCTCTTGTCAAAGCCAGTCATACGGAAAATTACAAACCAGCAGTTGAATCTTTGTCACGTGCTTTTAATTTGGC
AGAAAAAGCGGACGCTTCTGTTCAGGTGGACCCTAGCCTCTTTGAAAATGAGCAAGAAAACACTTTGTTTGCAGCTATTC
AAGGCTTGACTCTAGCAGGCAGTGCTGCCCAGCAGTTAGAACAGGTCTTTGCCCTTAGCCCAGTGATTAATGATTTCTTT
GATAACACAATGGTTATGGCAGGAGATCAAGCCCTTAAAAACAACCGTTTAGCTATTTTATCAGATTTAGTAAGCAAAGC
TAAGACCATTGTTGCTTTTAACCAACTAAACACCAAGTAA

Upstream 100 bases:

>100_bases
CTACGGACTATGTCAAAAAGACCAAGACTTCCTAGGTGCAAGCACCTGCGTCGCTTTGCTATTTTGATGGTGTCCGCTTA
ACGCCCTTGGTATCTTAATT

Downstream 100 bases:

>100_bases
ATAGAAAGGAAAGAGCCGTTGGGGACTTAGCTAGAAAAGTGAGTTCAGTACCCGAGTGGAAAAAACAAGGCCTTTAAACA
TTGCTTTTCACAGTGAGAGT

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS

Number of amino acids: Translated: 679; Mature: 678

Protein sequence:

>679_residues
MSKNLLIELGLEELPAYVVTPSEKQLGERLATFLTENRLSFEDIQTFSTPRRLAVRVSGLADQQTDLTEDFKGPAKKIAL
DADGNFSKAAQGFVRGKGLTTDAIEFREVKGEEYVYVTKHEAGKPAKEVLLGVTEVLSAMTFPVSMHWANNSFEYIRPVH
TLTVLLNDEALELDFLDIHSGRVSRGHRFLGTETTITSADSYEADLRSQFVIADAKERQEMIVEQIKTLEVEQGVQVDID
EDLLNEVLNLVEFPTAFMGSFEAKYLDVPEEVLVTSMKNHQRYFVVRDQAGHLMPNFVSVRNGNDQAIENVIKGNEKVLV
ARLEDGEFFWREDQKLQIADLVAKLTNVTFHEKIGSLAEHMDRTRVIAASLAKEANLSAEEVTAVDRAAQIYKFDLLTGM
VGEFDELQGIMGEKYALLAGEDAAVATAIREHYLPDAAGGALPETKVGAVLALAAKLDTLLSFFSVGLIPSGSNDPYALR
RATQGIVRILDHFGWRIPMDKLVDSLYDLSFDSLTYANKADVMNFIRARVDKMMGKAAPKDIREAILASSTFVVPEMLAA
AEALVKASHTENYKPAVESLSRAFNLAEKADASVQVDPSLFENEQENTLFAAIQGLTLAGSAAQQLEQVFALSPVINDFF
DNTMVMAGDQALKNNRLAILSDLVSKAKTIVAFNQLNTK

Sequences:

>Translated_679_residues
MSKNLLIELGLEELPAYVVTPSEKQLGERLATFLTENRLSFEDIQTFSTPRRLAVRVSGLADQQTDLTEDFKGPAKKIAL
DADGNFSKAAQGFVRGKGLTTDAIEFREVKGEEYVYVTKHEAGKPAKEVLLGVTEVLSAMTFPVSMHWANNSFEYIRPVH
TLTVLLNDEALELDFLDIHSGRVSRGHRFLGTETTITSADSYEADLRSQFVIADAKERQEMIVEQIKTLEVEQGVQVDID
EDLLNEVLNLVEFPTAFMGSFEAKYLDVPEEVLVTSMKNHQRYFVVRDQAGHLMPNFVSVRNGNDQAIENVIKGNEKVLV
ARLEDGEFFWREDQKLQIADLVAKLTNVTFHEKIGSLAEHMDRTRVIAASLAKEANLSAEEVTAVDRAAQIYKFDLLTGM
VGEFDELQGIMGEKYALLAGEDAAVATAIREHYLPDAAGGALPETKVGAVLALAAKLDTLLSFFSVGLIPSGSNDPYALR
RATQGIVRILDHFGWRIPMDKLVDSLYDLSFDSLTYANKADVMNFIRARVDKMMGKAAPKDIREAILASSTFVVPEMLAA
AEALVKASHTENYKPAVESLSRAFNLAEKADASVQVDPSLFENEQENTLFAAIQGLTLAGSAAQQLEQVFALSPVINDFF
DNTMVMAGDQALKNNRLAILSDLVSKAKTIVAFNQLNTK
>Mature_678_residues
SKNLLIELGLEELPAYVVTPSEKQLGERLATFLTENRLSFEDIQTFSTPRRLAVRVSGLADQQTDLTEDFKGPAKKIALD
ADGNFSKAAQGFVRGKGLTTDAIEFREVKGEEYVYVTKHEAGKPAKEVLLGVTEVLSAMTFPVSMHWANNSFEYIRPVHT
LTVLLNDEALELDFLDIHSGRVSRGHRFLGTETTITSADSYEADLRSQFVIADAKERQEMIVEQIKTLEVEQGVQVDIDE
DLLNEVLNLVEFPTAFMGSFEAKYLDVPEEVLVTSMKNHQRYFVVRDQAGHLMPNFVSVRNGNDQAIENVIKGNEKVLVA
RLEDGEFFWREDQKLQIADLVAKLTNVTFHEKIGSLAEHMDRTRVIAASLAKEANLSAEEVTAVDRAAQIYKFDLLTGMV
GEFDELQGIMGEKYALLAGEDAAVATAIREHYLPDAAGGALPETKVGAVLALAAKLDTLLSFFSVGLIPSGSNDPYALRR
ATQGIVRILDHFGWRIPMDKLVDSLYDLSFDSLTYANKADVMNFIRARVDKMMGKAAPKDIREAILASSTFVVPEMLAAA
EALVKASHTENYKPAVESLSRAFNLAEKADASVQVDPSLFENEQENTLFAAIQGLTLAGSAAQQLEQVFALSPVINDFFD
NTMVMAGDQALKNNRLAILSDLVSKAKTIVAFNQLNTK

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1789982, Length=681, Percent_Identity=35.6828193832599, Blast_Score=354, Evalue=9e-99,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYGB_STRP1 (Q99YI4)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_269722.1
- RefSeq:   YP_282747.1
- ProteinModelPortal:   Q99YI4
- EnsemblBacteria:   EBSTRT00000001189
- EnsemblBacteria:   EBSTRT00000028105
- GeneID:   3571499
- GeneID:   901933
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_1688
- KEGG:   spz:M5005_Spy_1384
- GeneTree:   EBGT00050000028086
- HOGENOM:   HBG586599
- OMA:   FIRPVHT
- ProtClustDB:   PRK01233
- BioCyc:   SPYO160490:SPY1688-MONOMER
- BioCyc:   SPYO293653:M5005_SPY1384-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00255
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311
- PRINTS:   PR01045
- TIGRFAMs:   TIGR00211

Pfam domain/function: PF02092 tRNA_synt_2f

EC number: =6.1.1.14

Molecular weight: Translated: 74931; Mature: 74800

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKNLLIELGLEELPAYVVTPSEKQLGERLATFLTENRLSFEDIQTFSTPRRLAVRVSGL
CCCCEEEEECHHHCCCEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHEEEEECCC
ADQQTDLTEDFKGPAKKIALDADGNFSKAAQGFVRGKGLTTDAIEFREVKGEEYVYVTKH
CCCCCCCHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEECC
EAGKPAKEVLLGVTEVLSAMTFPVSMHWANNSFEYIRPVHTLTVLLNDEALELDFLDIHS
CCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHCCEEEEEEEECCCEEEEEEEECCC
GRVSRGHRFLGTETTITSADSYEADLRSQFVIADAKERQEMIVEQIKTLEVEQGVQVDID
CCCCCCCEEECCCCEEECCCCCCHHHHCCEEEECHHHHHHHHHHHHHHHHHCCCCEEECC
EDLLNEVLNLVEFPTAFMGSFEAKYLDVPEEVLVTSMKNHQRYFVVRDQAGHLMPNFVSV
HHHHHHHHHHHHCCHHHHCCCCCHHCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCEEEE
RNGNDQAIENVIKGNEKVLVARLEDGEFFWREDQKLQIADLVAKLTNVTFHEKIGSLAEH
CCCCHHHHHHHHCCCCEEEEEEECCCCEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHH
MDRTRVIAASLAKEANLSAEEVTAVDRAAQIYKFDLLTGMVGEFDELQGIMGEKYALLAG
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEEC
EDAAVATAIREHYLPDAAGGALPETKVGAVLALAAKLDTLLSFFSVGLIPSGSNDPYALR
CCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
RATQGIVRILDHFGWRIPMDKLVDSLYDLSFDSLTYANKADVMNFIRARVDKMMGKAAPK
HHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCH
DIREAILASSTFVVPEMLAAAEALVKASHTENYKPAVESLSRAFNLAEKADASVQVDPSL
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEECHHH
FENEQENTLFAAIQGLTLAGSAAQQLEQVFALSPVINDFFDNTMVMAGDQALKNNRLAIL
HCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHCCCHHHH
SDLVSKAKTIVAFNQLNTK
HHHHHHHHHEEEECCCCCC
>Mature Secondary Structure 
SKNLLIELGLEELPAYVVTPSEKQLGERLATFLTENRLSFEDIQTFSTPRRLAVRVSGL
CCCEEEEECHHHCCCEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHEEEEECCC
ADQQTDLTEDFKGPAKKIALDADGNFSKAAQGFVRGKGLTTDAIEFREVKGEEYVYVTKH
CCCCCCCHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEECC
EAGKPAKEVLLGVTEVLSAMTFPVSMHWANNSFEYIRPVHTLTVLLNDEALELDFLDIHS
CCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHCCEEEEEEEECCCEEEEEEEECCC
GRVSRGHRFLGTETTITSADSYEADLRSQFVIADAKERQEMIVEQIKTLEVEQGVQVDID
CCCCCCCEEECCCCEEECCCCCCHHHHCCEEEECHHHHHHHHHHHHHHHHHCCCCEEECC
EDLLNEVLNLVEFPTAFMGSFEAKYLDVPEEVLVTSMKNHQRYFVVRDQAGHLMPNFVSV
HHHHHHHHHHHHCCHHHHCCCCCHHCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCEEEE
RNGNDQAIENVIKGNEKVLVARLEDGEFFWREDQKLQIADLVAKLTNVTFHEKIGSLAEH
CCCCHHHHHHHHCCCCEEEEEEECCCCEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHH
MDRTRVIAASLAKEANLSAEEVTAVDRAAQIYKFDLLTGMVGEFDELQGIMGEKYALLAG
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEEC
EDAAVATAIREHYLPDAAGGALPETKVGAVLALAAKLDTLLSFFSVGLIPSGSNDPYALR
CCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
RATQGIVRILDHFGWRIPMDKLVDSLYDLSFDSLTYANKADVMNFIRARVDKMMGKAAPK
HHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCH
DIREAILASSTFVVPEMLAAAEALVKASHTENYKPAVESLSRAFNLAEKADASVQVDPSL
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEECHHH
FENEQENTLFAAIQGLTLAGSAAQQLEQVFALSPVINDFFDNTMVMAGDQALKNNRLAIL
HCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHCCCHHHH
SDLVSKAKTIVAFNQLNTK
HHHHHHHHHEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296