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The map label for this gene is proA

Identifier: 15675534

GI number: 15675534

Start: 1384982

End: 1386232

Strand: Reverse

Name: proA

Synonym: SPy_1670

Alternate gene names: 15675534

Gene position: 1386232-1384982 (Counterclockwise)

Preceding gene: 15675535

Following gene: 15675533

Centisome position: 74.83

GC content: 42.29

Gene sequence:

>1251_bases
ATGACTGATATGAGAAGGTTAGGGCAAAGGGCAAAACAAGCTAGTCTTTTAATAGCACCTTTGTCGACGCAAATTAAAAA
TCGCTTTTTAAGCACCTTAGCAAAGGCATTAGTTGACGATACACAGACTCTCTTAGCCGCTAACCAAAAAGATTTAGCGA
ATGCTAAAGAACATGGTATTTCTGACATCATGATGGATCGCTTGCGCTTAACTTCAGAACGGATTAAAGCGATAGCACAG
GGAGTTCAACAGGTTGCTGACTTAGCTGATCCAATCGGACAAGTCATTAAGGGCTATACTAACCTCGATGGCCTTAAAAT
CCTCCAAAAGAGAGTTCCTCTAGGGGTGATTGCGATGATTTTTGAGAGTCGTCCGAATGTTTCAGTGGATGCTTTTAGTC
TGGCGTTCAAAACCAATAATGCGATCATCCTAAGGGGAGGAAAAGATGCCCTTCACTCAAATAAAGCTTTGGTGAAGTTG
ATCCGACAAAGTCTGGAAAAATCAGGGATCACCCCAGATGCTGTCCAGCTAGTTGAGGATCCCTCACATGCTGTCGCTGA
AGAATTGATGCAGGCAACGGATTATGTAGATGTTTTGATTCCACGTGGTGGTGCTAAATTAATTCAGACCGTTAAGGAAA
AAGCAAAAGTGCCTGTCATCGAAACAGGTGTGGGGAATGTGCATATCTATGTGGATGCTCAAGCTGACTTAGATATAGCT
ACTAAGATTGTTATCAACGCTAAAACAAAGCGCCCAAGTGTTTGCAATGCAGCTGAGGGATTGGTAATTCACGAAGCTGT
AGCAGCAAGGTTTATTCCTATGCTTGAAAAAGCGATCAATCAAGTTCAGCCAGTAGAATGGCGGGCAGATGACAAGGCTT
TGCCGTTATTTGAGCAGGCAGTGCCTGCAAAAGCAGAGGATTTTGAGACCGAATTTTTAGATTATATCATGTCTGTTAAG
GTTGTTTCCTCTTTGGAAGAAGCTATTTCTTGGATAAATCAGTATACTAGTCACCATTCCGAAGCTATCATCACAAGGGA
TATTAAGGCAGCAGAGACCTTTCAAGATTTAGTGGATGCTGCTGCTGTTTATGTCAACGCTTCAACACGATTTACCGATG
GTTTTGTTTTTGGGCTTGGTGCTGAAATTGGGATTTCTACTCAAAAAATGCACGCCCGTGGGCCAATGGGCTTAGAAGCC
TTGACCAGCACTAAATTTTATATTAATGGGGATGGACATATCAGAGAATAA

Upstream 100 bases:

>100_bases
CAGATGGTCTTGATGAATGGAGCTAATCCCAGAGATATTTTAAGAGTGCTTGAAGGGCAACCACTAGGCACCTGGTTCAA
GCAGATAGAAGAGGTAAGGC

Downstream 100 bases:

>100_bases
GCTACTATAAGGTCCCTTAATGACTAATGAAAGAGAAAAAGAAGAAGCAAAGGTTACTTTAGAAGAACTTTTACATCAGA
TTCAGGTGATTCATTTGGAA

Product: gamma-glutamyl phosphate reductase

Products: NA

Alternate protein names: GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSA dehydrogenase

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MTDMRRLGQRAKQASLLIAPLSTQIKNRFLSTLAKALVDDTQTLLAANQKDLANAKEHGISDIMMDRLRLTSERIKAIAQ
GVQQVADLADPIGQVIKGYTNLDGLKILQKRVPLGVIAMIFESRPNVSVDAFSLAFKTNNAIILRGGKDALHSNKALVKL
IRQSLEKSGITPDAVQLVEDPSHAVAEELMQATDYVDVLIPRGGAKLIQTVKEKAKVPVIETGVGNVHIYVDAQADLDIA
TKIVINAKTKRPSVCNAAEGLVIHEAVAARFIPMLEKAINQVQPVEWRADDKALPLFEQAVPAKAEDFETEFLDYIMSVK
VVSSLEEAISWINQYTSHHSEAIITRDIKAAETFQDLVDAAAVYVNASTRFTDGFVFGLGAEIGISTQKMHARGPMGLEA
LTSTKFYINGDGHIRE

Sequences:

>Translated_416_residues
MTDMRRLGQRAKQASLLIAPLSTQIKNRFLSTLAKALVDDTQTLLAANQKDLANAKEHGISDIMMDRLRLTSERIKAIAQ
GVQQVADLADPIGQVIKGYTNLDGLKILQKRVPLGVIAMIFESRPNVSVDAFSLAFKTNNAIILRGGKDALHSNKALVKL
IRQSLEKSGITPDAVQLVEDPSHAVAEELMQATDYVDVLIPRGGAKLIQTVKEKAKVPVIETGVGNVHIYVDAQADLDIA
TKIVINAKTKRPSVCNAAEGLVIHEAVAARFIPMLEKAINQVQPVEWRADDKALPLFEQAVPAKAEDFETEFLDYIMSVK
VVSSLEEAISWINQYTSHHSEAIITRDIKAAETFQDLVDAAAVYVNASTRFTDGFVFGLGAEIGISTQKMHARGPMGLEA
LTSTKFYINGDGHIRE
>Mature_415_residues
TDMRRLGQRAKQASLLIAPLSTQIKNRFLSTLAKALVDDTQTLLAANQKDLANAKEHGISDIMMDRLRLTSERIKAIAQG
VQQVADLADPIGQVIKGYTNLDGLKILQKRVPLGVIAMIFESRPNVSVDAFSLAFKTNNAIILRGGKDALHSNKALVKLI
RQSLEKSGITPDAVQLVEDPSHAVAEELMQATDYVDVLIPRGGAKLIQTVKEKAKVPVIETGVGNVHIYVDAQADLDIAT
KIVINAKTKRPSVCNAAEGLVIHEAVAARFIPMLEKAINQVQPVEWRADDKALPLFEQAVPAKAEDFETEFLDYIMSVKV
VSSLEEAISWINQYTSHHSEAIITRDIKAAETFQDLVDAAAVYVNASTRFTDGFVFGLGAEIGISTQKMHARGPMGLEAL
TSTKFYINGDGHIRE

Specific function: Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate

COG id: COG0014

COG function: function code E; Gamma-glutamyl phosphate reductase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyl phosphate reductase family

Homologues:

Organism=Homo sapiens, GI21361368, Length=411, Percent_Identity=35.7664233576642, Blast_Score=262, Evalue=5e-70,
Organism=Homo sapiens, GI62912457, Length=411, Percent_Identity=35.7664233576642, Blast_Score=262, Evalue=5e-70,
Organism=Escherichia coli, GI1786438, Length=415, Percent_Identity=46.2650602409639, Blast_Score=382, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17569969, Length=417, Percent_Identity=39.0887290167866, Blast_Score=282, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI17569967, Length=417, Percent_Identity=39.0887290167866, Blast_Score=282, Evalue=3e-76,
Organism=Saccharomyces cerevisiae, GI6324899, Length=434, Percent_Identity=35.0230414746544, Blast_Score=268, Evalue=1e-72,
Organism=Drosophila melanogaster, GI21357643, Length=423, Percent_Identity=36.1702127659575, Blast_Score=256, Evalue=2e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROA_STRP1 (Q99YJ8)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_269708.1
- RefSeq:   YP_282733.1
- ProteinModelPortal:   Q99YJ8
- SMR:   Q99YJ8
- EnsemblBacteria:   EBSTRT00000000478
- EnsemblBacteria:   EBSTRT00000028325
- GeneID:   3571524
- GeneID:   901918
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_1670
- KEGG:   spz:M5005_Spy_1370
- GeneTree:   EBGT00050000027638
- HOGENOM:   HBG318080
- OMA:   QYPAACN
- ProtClustDB:   PRK00197
- BioCyc:   SPYO160490:SPY1670-MONOMER
- BioCyc:   SPYO293653:M5005_SPY1370-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00412
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR000965
- InterPro:   IPR020593
- InterPro:   IPR012134
- Gene3D:   G3DSA:3.40.309.10
- Gene3D:   G3DSA:3.40.605.10
- PANTHER:   PTHR11063:SF1
- PIRSF:   PIRSF000151
- TIGRFAMs:   TIGR00407

Pfam domain/function: PF00171 Aldedh; SSF53720 Aldehyde_DH/Histidinol_DH

EC number: =1.2.1.41

Molecular weight: Translated: 45430; Mature: 45299

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS01223 PROA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDMRRLGQRAKQASLLIAPLSTQIKNRFLSTLAKALVDDTQTLLAANQKDLANAKEHGI
CCHHHHHHHHHHHHCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCH
SDIMMDRLRLTSERIKAIAQGVQQVADLADPIGQVIKGYTNLDGLKILQKRVPLGVIAMI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHH
FESRPNVSVDAFSLAFKTNNAIILRGGKDALHSNKALVKLIRQSLEKSGITPDAVQLVED
HHCCCCCCEEEEEEEEECCCEEEEECCHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHCC
PSHAVAEELMQATDYVDVLIPRGGAKLIQTVKEKAKVPVIETGVGNVHIYVDAQADLDIA
CCHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHCCCCEEEECCCEEEEEEECCCCCCEE
TKIVINAKTKRPSVCNAAEGLVIHEAVAARFIPMLEKAINQVQPVEWRADDKALPLFEQA
EEEEEECCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHH
VPAKAEDFETEFLDYIMSVKVVSSLEEAISWINQYTSHHSEAIITRDIKAAETFQDLVDA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHH
AAVYVNASTRFTDGFVFGLGAEIGISTQKMHARGPMGLEALTSTKFYINGDGHIRE
HHHEEECCCEECCCEEEECCCCCCCCHHHHHCCCCCCHHHHHCEEEEECCCCCCCC
>Mature Secondary Structure 
TDMRRLGQRAKQASLLIAPLSTQIKNRFLSTLAKALVDDTQTLLAANQKDLANAKEHGI
CHHHHHHHHHHHHCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCH
SDIMMDRLRLTSERIKAIAQGVQQVADLADPIGQVIKGYTNLDGLKILQKRVPLGVIAMI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHH
FESRPNVSVDAFSLAFKTNNAIILRGGKDALHSNKALVKLIRQSLEKSGITPDAVQLVED
HHCCCCCCEEEEEEEEECCCEEEEECCHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHCC
PSHAVAEELMQATDYVDVLIPRGGAKLIQTVKEKAKVPVIETGVGNVHIYVDAQADLDIA
CCHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHCCCCEEEECCCEEEEEEECCCCCCEE
TKIVINAKTKRPSVCNAAEGLVIHEAVAARFIPMLEKAINQVQPVEWRADDKALPLFEQA
EEEEEECCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHH
VPAKAEDFETEFLDYIMSVKVVSSLEEAISWINQYTSHHSEAIITRDIKAAETFQDLVDA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHH
AAVYVNASTRFTDGFVFGLGAEIGISTQKMHARGPMGLEALTSTKFYINGDGHIRE
HHHEEECCCEECCCEEEECCCCCCCCHHHHHCCCCCCHHHHHCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296