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The map label for this gene is proB [H]

Identifier: 15675535

GI number: 15675535

Start: 1386225

End: 1387046

Strand: Reverse

Name: proB [H]

Synonym: SPy_1672

Alternate gene names: 15675535

Gene position: 1387046-1386225 (Counterclockwise)

Preceding gene: 15675536

Following gene: 15675534

Centisome position: 74.88

GC content: 39.54

Gene sequence:

>822_bases
ATGATGAAACGACAATTTGAAGATGTAACACGTATCGTAATTAAAATTGGCACTAGTTCATTAGTGCTACCAACAGGTAA
AATTAATTTAGAAAAAATCGACCAACTGGCTTTTGTGATTTCAAGTTTGATGAATAAGGGCAAGGAAGTTATTTTAGTAT
CATCGGGAGCGATGGGATTTGGACTAGATATTTTGAAAATGGAAAAACGTCCGACCAATCTTGCTAAACAACAGGCAGTT
TCCAGTGTTGGTCAGGTAGCCATGATGAGTCTTTATTCACAAATTTTTGCATACTACCAAACCAACGTGTCACAAATTTT
ATTGACGAGAGATGTGGTGGTTTTCCCAGAAAGTCTAGCCAATGTTACCAATGCTTTTGAAAGTCTGATTAGTTTGGGCA
TTGTGCCGATTGTTAATGAAAACGATGCGGTTAGTGTGGATGAAATGGATCATGCGACTAAATTTGGAGATAATGACCGT
TTATCTGCTGTTGTTGCTGGTATTACAAAAGCTGATTTATTAATCATGTTGTCTGATATTGATGGCCTTTTTGATAAAAA
CCCTACTATTTATGAGGATGCGCAGCTACGCAGCCATGTTGCTAATATTACGCAAGAAATCATTGCGTCAGCAGGTGGTG
CGGGCAGTAAGTTTGGCACAGGTGGGATGCTAAGTAAGGTGCAGTCTGCTCAGATGGTATTTGAAAACAAAGGACAGATG
GTCTTGATGAATGGAGCTAATCCCAGAGATATTTTAAGAGTGCTTGAAGGGCAACCACTAGGCACCTGGTTCAAGCAGAT
AGAAGAGGTAAGGCATGACTGA

Upstream 100 bases:

>100_bases
CAAAAAACCTTCTGGAAAAGCTTAGAGAGGCTTTAACGAAGAGGGATCATTATTCGGAAAAACAGTCCTCTGGGGCTGTT
TTTTATGCTATAATTTGGGT

Downstream 100 bases:

>100_bases
TATGAGAAGGTTAGGGCAAAGGGCAAAACAAGCTAGTCTTTTAATAGCACCTTTGTCGACGCAAATTAAAAATCGCTTTT
TAAGCACCTTAGCAAAGGCA

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MMKRQFEDVTRIVIKIGTSSLVLPTGKINLEKIDQLAFVISSLMNKGKEVILVSSGAMGFGLDILKMEKRPTNLAKQQAV
SSVGQVAMMSLYSQIFAYYQTNVSQILLTRDVVVFPESLANVTNAFESLISLGIVPIVNENDAVSVDEMDHATKFGDNDR
LSAVVAGITKADLLIMLSDIDGLFDKNPTIYEDAQLRSHVANITQEIIASAGGAGSKFGTGGMLSKVQSAQMVFENKGQM
VLMNGANPRDILRVLEGQPLGTWFKQIEEVRHD

Sequences:

>Translated_273_residues
MMKRQFEDVTRIVIKIGTSSLVLPTGKINLEKIDQLAFVISSLMNKGKEVILVSSGAMGFGLDILKMEKRPTNLAKQQAV
SSVGQVAMMSLYSQIFAYYQTNVSQILLTRDVVVFPESLANVTNAFESLISLGIVPIVNENDAVSVDEMDHATKFGDNDR
LSAVVAGITKADLLIMLSDIDGLFDKNPTIYEDAQLRSHVANITQEIIASAGGAGSKFGTGGMLSKVQSAQMVFENKGQM
VLMNGANPRDILRVLEGQPLGTWFKQIEEVRHD
>Mature_273_residues
MMKRQFEDVTRIVIKIGTSSLVLPTGKINLEKIDQLAFVISSLMNKGKEVILVSSGAMGFGLDILKMEKRPTNLAKQQAV
SSVGQVAMMSLYSQIFAYYQTNVSQILLTRDVVVFPESLANVTNAFESLISLGIVPIVNENDAVSVDEMDHATKFGDNDR
LSAVVAGITKADLLIMLSDIDGLFDKNPTIYEDAQLRSHVANITQEIIASAGGAGSKFGTGGMLSKVQSAQMVFENKGQM
VLMNGANPRDILRVLEGQPLGTWFKQIEEVRHD

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate 5-kinase family [H]

Homologues:

Organism=Homo sapiens, GI62912457, Length=297, Percent_Identity=29.96632996633, Blast_Score=119, Evalue=2e-27,
Organism=Homo sapiens, GI21361368, Length=299, Percent_Identity=30.1003344481605, Blast_Score=118, Evalue=5e-27,
Organism=Escherichia coli, GI1786437, Length=260, Percent_Identity=34.2307692307692, Blast_Score=146, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17569967, Length=275, Percent_Identity=31.2727272727273, Blast_Score=127, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17569969, Length=275, Percent_Identity=31.2727272727273, Blast_Score=127, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6320506, Length=250, Percent_Identity=36, Blast_Score=159, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6321822, Length=248, Percent_Identity=33.8709677419355, Blast_Score=153, Evalue=3e-38,
Organism=Drosophila melanogaster, GI21357643, Length=297, Percent_Identity=31.986531986532, Blast_Score=134, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797 [H]

Pfam domain/function: PF00696 AA_kinase [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 29757; Mature: 29757

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKRQFEDVTRIVIKIGTSSLVLPTGKINLEKIDQLAFVISSLMNKGKEVILVSSGAMGF
CCCHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
GLDILKMEKRPTNLAKQQAVSSVGQVAMMSLYSQIFAYYQTNVSQILLTRDVVVFPESLA
CHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHEECCHHHH
NVTNAFESLISLGIVPIVNENDAVSVDEMDHATKFGDNDRLSAVVAGITKADLLIMLSDI
HHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEEEHHH
DGLFDKNPTIYEDAQLRSHVANITQEIIASAGGAGSKFGTGGMLSKVQSAQMVFENKGQM
HHHHCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCE
VLMNGANPRDILRVLEGQPLGTWFKQIEEVRHD
EEECCCCHHHHHHHHCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MMKRQFEDVTRIVIKIGTSSLVLPTGKINLEKIDQLAFVISSLMNKGKEVILVSSGAMGF
CCCHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
GLDILKMEKRPTNLAKQQAVSSVGQVAMMSLYSQIFAYYQTNVSQILLTRDVVVFPESLA
CHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHEECCHHHH
NVTNAFESLISLGIVPIVNENDAVSVDEMDHATKFGDNDRLSAVVAGITKADLLIMLSDI
HHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEEEHHH
DGLFDKNPTIYEDAQLRSHVANITQEIIASAGGAGSKFGTGGMLSKVQSAQMVFENKGQM
HHHHCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCE
VLMNGANPRDILRVLEGQPLGTWFKQIEEVRHD
EEECCCCHHHHHHHHCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA