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The map label for this gene is aapA [H]

Identifier: 15675525

GI number: 15675525

Start: 1373797

End: 1375176

Strand: Reverse

Name: aapA [H]

Synonym: SPy_1654

Alternate gene names: 15675525

Gene position: 1375176-1373797 (Counterclockwise)

Preceding gene: 15675526

Following gene: 15675524

Centisome position: 74.24

GC content: 39.86

Gene sequence:

>1380_bases
ATGTCTATAAAAGAACAAACAGACAATAACGAACTAGAAAATGGAATGGTAAGGGGCTTAGAAAATCGTCATGTGCAGTT
AATTGCGATCGCAGGAACGATTGGAACAGGTCTCTTTTTAGGGGCAGGACGCTCTATTGCCCTAACAGGACCATCTATTA
TTTTTGTGTATATGATTACAGGTGCCTTCATGTTTATGATGATGCGTGCCATCGGTGAAATGCTCTATTATGATCCAGAT
CAACACACCTTTATCAATTTTATTTCCAAATACATTGGTCCAGGCTGGGGTTATTTTTCGGGTCTATCCTATTGGATTTC
CCTTATTTTTATTGGAATGGCAGAAATCACAGCAGTAGGTGCTTATGTGCAATTTTGGTTCCCAAGCTGGCCAGCCTGGT
TGATTCAGTTGGTTTTCTTAGTCTTACTTAGTTCGATCAACTTAATTGCTGTGCGCGTTTTTGGGGAAACAGAGTTTTGG
TTTGCCATGATTAAGATTTTAGCTATTTTAGCTTTGATTGCAACAGCTATTTTCATGGTATTGACAGGTTTTGAAACTCA
CACAGGCCATGCTAGCCTTTCCAATATTTTTGACCATTTTTCCATGTTCCCAAATGGGAAACTAAAGTTCTTTATGGCCT
TCCAAATGGTTTTCTTTGCTTATCAAGCTATCGAATTTGTGGGAATTACCACTTCTGAGACGGCTAACCCAAGAAAAGTT
CTACCAAAGGCTATTCAAGAAATTCCAACCCGTATTGTGATCTTTTATGTGGGAGCCTTGGTCTCTATTATGGCAATTGT
GCCATGGCATCAGTTACCAGTTGATGAATCTCCTTTTGTGATGGTGTTCAAATTGATTGGTATTAAATGGGCAGCAGCCT
TGATTAACTTTGTGGTCTTGACATCAGCAGCGTCAGCGCTTAACTCAACCCTTTATTCAACCGGTCGTCATCTCTATCAG
ATTGCTAATGAGACTCCAAACGCTTTGACCAATCGCTTAAAGATTAATACTTTATCTCGACAAGGGGTGCCAAGTCGTGC
CATTATTGCTTCAGCGGTTGTGGTTGGTATCTCAGCTTTGATTAATATCCTACCAGGAGTTGCAGATGCGTTCTCACTCA
TTACGGCCTCCTCATCAGGTGTATATATTGCTATCTACGCCTTAACCATGATTGCTCACTGGAAGTACCGTCAATCTAAG
GACTTTATGGCAGATGGTTATTTGATGCCAAAATATAAAGTGACAACGCCTTTGACCCTTGCTTTCTTTGCCTTTGTCTT
TATCTCTCTTTTCTTACAAGAATCCACCTATATCGGCGCCATTGGAGCAACGATTTGGATTATTATTTTTGGGATTTATA
GCAATGTCAAATTTAAATAA

Upstream 100 bases:

>100_bases
ATATAAAGAACAGTTTTCCTTAATATCTTTCCTAGATAAATGTTGAAAATTTTCTGAAAAAAGGATATAATAAATAGTAT
TTTAGTGAATGGAGTTTAGA

Downstream 100 bases:

>100_bases
TAATTAGCGATATAGAACTGAATCCATTAACTCTTTCTTATTTGAGGATGTCATGATGGATCAGTTCTTTTTTTACTTTA
GCGATAACTCAAGTTATTTC

Product: putative amino acid permease

Products: D-glycine [Cytoplasm]; D-alanine [Cytoplasm]; Proton [Cytoplasm]; D-serine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MSIKEQTDNNELENGMVRGLENRHVQLIAIAGTIGTGLFLGAGRSIALTGPSIIFVYMITGAFMFMMMRAIGEMLYYDPD
QHTFINFISKYIGPGWGYFSGLSYWISLIFIGMAEITAVGAYVQFWFPSWPAWLIQLVFLVLLSSINLIAVRVFGETEFW
FAMIKILAILALIATAIFMVLTGFETHTGHASLSNIFDHFSMFPNGKLKFFMAFQMVFFAYQAIEFVGITTSETANPRKV
LPKAIQEIPTRIVIFYVGALVSIMAIVPWHQLPVDESPFVMVFKLIGIKWAAALINFVVLTSAASALNSTLYSTGRHLYQ
IANETPNALTNRLKINTLSRQGVPSRAIIASAVVVGISALINILPGVADAFSLITASSSGVYIAIYALTMIAHWKYRQSK
DFMADGYLMPKYKVTTPLTLAFFAFVFISLFLQESTYIGAIGATIWIIIFGIYSNVKFK

Sequences:

>Translated_459_residues
MSIKEQTDNNELENGMVRGLENRHVQLIAIAGTIGTGLFLGAGRSIALTGPSIIFVYMITGAFMFMMMRAIGEMLYYDPD
QHTFINFISKYIGPGWGYFSGLSYWISLIFIGMAEITAVGAYVQFWFPSWPAWLIQLVFLVLLSSINLIAVRVFGETEFW
FAMIKILAILALIATAIFMVLTGFETHTGHASLSNIFDHFSMFPNGKLKFFMAFQMVFFAYQAIEFVGITTSETANPRKV
LPKAIQEIPTRIVIFYVGALVSIMAIVPWHQLPVDESPFVMVFKLIGIKWAAALINFVVLTSAASALNSTLYSTGRHLYQ
IANETPNALTNRLKINTLSRQGVPSRAIIASAVVVGISALINILPGVADAFSLITASSSGVYIAIYALTMIAHWKYRQSK
DFMADGYLMPKYKVTTPLTLAFFAFVFISLFLQESTYIGAIGATIWIIIFGIYSNVKFK
>Mature_458_residues
SIKEQTDNNELENGMVRGLENRHVQLIAIAGTIGTGLFLGAGRSIALTGPSIIFVYMITGAFMFMMMRAIGEMLYYDPDQ
HTFINFISKYIGPGWGYFSGLSYWISLIFIGMAEITAVGAYVQFWFPSWPAWLIQLVFLVLLSSINLIAVRVFGETEFWF
AMIKILAILALIATAIFMVLTGFETHTGHASLSNIFDHFSMFPNGKLKFFMAFQMVFFAYQAIEFVGITTSETANPRKVL
PKAIQEIPTRIVIFYVGALVSIMAIVPWHQLPVDESPFVMVFKLIGIKWAAALINFVVLTSAASALNSTLYSTGRHLYQI
ANETPNALTNRLKINTLSRQGVPSRAIIASAVVVGISALINILPGVADAFSLITASSSGVYIAIYALTMIAHWKYRQSKD
FMADGYLMPKYKVTTPLTLAFFAFVFISLFLQESTYIGAIGATIWIIIFGIYSNVKFK

Specific function: Probable amino-acid or metabolite transport protein [H]

COG id: COG1113

COG function: function code E; Gamma-aminobutyrate permease and related permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily [H]

Homologues:

Organism=Escherichia coli, GI1790653, Length=451, Percent_Identity=41.9068736141907, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI1786302, Length=454, Percent_Identity=36.5638766519824, Blast_Score=300, Evalue=1e-82,
Organism=Escherichia coli, GI1786789, Length=456, Percent_Identity=34.6491228070175, Blast_Score=298, Evalue=6e-82,
Organism=Escherichia coli, GI48994972, Length=437, Percent_Identity=37.5286041189931, Blast_Score=281, Evalue=5e-77,
Organism=Escherichia coli, GI1786602, Length=446, Percent_Identity=32.2869955156951, Blast_Score=251, Evalue=6e-68,
Organism=Escherichia coli, GI87081915, Length=395, Percent_Identity=35.1898734177215, Blast_Score=244, Evalue=6e-66,
Organism=Escherichia coli, GI87081708, Length=406, Percent_Identity=33.0049261083744, Blast_Score=220, Evalue=1e-58,
Organism=Escherichia coli, GI1788480, Length=467, Percent_Identity=29.5503211991435, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1789017, Length=431, Percent_Identity=30.1624129930394, Blast_Score=183, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI17540018, Length=421, Percent_Identity=26.3657957244656, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6324061, Length=484, Percent_Identity=27.8925619834711, Blast_Score=169, Evalue=8e-43,
Organism=Saccharomyces cerevisiae, GI6324924, Length=428, Percent_Identity=28.5046728971963, Blast_Score=167, Evalue=5e-42,
Organism=Saccharomyces cerevisiae, GI6320772, Length=368, Percent_Identity=31.7934782608696, Blast_Score=166, Evalue=6e-42,
Organism=Saccharomyces cerevisiae, GI6324990, Length=390, Percent_Identity=30.2564102564103, Blast_Score=159, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6324059, Length=446, Percent_Identity=29.8206278026906, Blast_Score=155, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6321053, Length=413, Percent_Identity=27.6029055690073, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6322892, Length=321, Percent_Identity=29.9065420560748, Blast_Score=136, Evalue=7e-33,
Organism=Saccharomyces cerevisiae, GI6324553, Length=330, Percent_Identity=29.0909090909091, Blast_Score=130, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6319608, Length=371, Percent_Identity=26.4150943396226, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6321629, Length=415, Percent_Identity=25.0602409638554, Blast_Score=119, Evalue=8e-28,
Organism=Saccharomyces cerevisiae, GI6320251, Length=445, Percent_Identity=24.0449438202247, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6319542, Length=439, Percent_Identity=25.5125284738041, Blast_Score=107, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6320717, Length=420, Percent_Identity=23.5714285714286, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6319824, Length=432, Percent_Identity=23.6111111111111, Blast_Score=104, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6319543, Length=423, Percent_Identity=25.531914893617, Blast_Score=104, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6322967, Length=319, Percent_Identity=26.9592476489028, Blast_Score=101, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6324981, Length=407, Percent_Identity=24.5700245700246, Blast_Score=87, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6320364, Length=501, Percent_Identity=22.7544910179641, Blast_Score=78, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293
- InterPro:   IPR004840 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 50974; Mature: 50843

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: PS00218 AMINO_ACID_PERMEASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIKEQTDNNELENGMVRGLENRHVQLIAIAGTIGTGLFLGAGRSIALTGPSIIFVYMIT
CCCCCCCCCCHHHHHHHCCCCCCCEEEEEEECHHHHHHHCCCCCEEEECCCHHHHHHHHH
GAFMFMMMRAIGEMLYYDPDQHTFINFISKYIGPGWGYFSGLSYWISLIFIGMAEITAVG
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AYVQFWFPSWPAWLIQLVFLVLLSSINLIAVRVFGETEFWFAMIKILAILALIATAIFMV
HHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
LTGFETHTGHASLSNIFDHFSMFPNGKLKFFMAFQMVFFAYQAIEFVGITTSETANPRKV
HHCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHH
LPKAIQEIPTRIVIFYVGALVSIMAIVPWHQLPVDESPFVMVFKLIGIKWAAALINFVVL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
TSAASALNSTLYSTGRHLYQIANETPNALTNRLKINTLSRQGVPSRAIIASAVVVGISAL
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHH
INILPGVADAFSLITASSSGVYIAIYALTMIAHWKYRQSKDFMADGYLMPKYKVTTPLTL
HHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEECCCEECCCHHHH
AFFAFVFISLFLQESTYIGAIGATIWIIIFGIYSNVKFK
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SIKEQTDNNELENGMVRGLENRHVQLIAIAGTIGTGLFLGAGRSIALTGPSIIFVYMIT
CCCCCCCCCHHHHHHHCCCCCCCEEEEEEECHHHHHHHCCCCCEEEECCCHHHHHHHHH
GAFMFMMMRAIGEMLYYDPDQHTFINFISKYIGPGWGYFSGLSYWISLIFIGMAEITAVG
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AYVQFWFPSWPAWLIQLVFLVLLSSINLIAVRVFGETEFWFAMIKILAILALIATAIFMV
HHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
LTGFETHTGHASLSNIFDHFSMFPNGKLKFFMAFQMVFFAYQAIEFVGITTSETANPRKV
HHCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHH
LPKAIQEIPTRIVIFYVGALVSIMAIVPWHQLPVDESPFVMVFKLIGIKWAAALINFVVL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
TSAASALNSTLYSTGRHLYQIANETPNALTNRLKINTLSRQGVPSRAIIASAVVVGISAL
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHH
INILPGVADAFSLITASSSGVYIAIYALTMIAHWKYRQSKDFMADGYLMPKYKVTTPLTL
HHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEECCCEECCCHHHH
AFFAFVFISLFLQESTYIGAIGATIWIIIFGIYSNVKFK
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-glycine [Periplasm]; D-alanine [Periplasm]; Proton [Periplasm]; D-serine [Periplasm] [C]

Specific reaction: Proton [Periplasm] + D-glycine [Periplasm] = Proton [Cytoplasm] + D-glycine [Cytoplasm] Proton [Periplasm] + D-alanine [Periplasm] = Proton [Cytoplasm] + D-alanine [Cytoplasm] Proton [Periplasm] + D-serine [Periplasm] = Proton [Cytoplasm] + D-serine [Cyto

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]