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The map label for this gene is fmt

Identifier: 15675504

GI number: 15675504

Start: 1349684

End: 1350619

Strand: Reverse

Name: fmt

Synonym: SPy_1628

Alternate gene names: 15675504

Gene position: 1350619-1349684 (Counterclockwise)

Preceding gene: 15675505

Following gene: 15675503

Centisome position: 72.91

GC content: 38.14

Gene sequence:

>936_bases
ATGATAAAATTATTATTTATGGGAACTCCCCAATTTTCAGCAACTGTATTGAAGGGCTTGCTAGATAATCCTGCCTATGA
GATTTTAGGAGTGGTTACTCAGCCTGATCGAGCTGTTGGACGCAAAAAAGATATTAAGGTAACACCTGTAAAGCAATTAG
CTCTTGAGCATGGAATATCAATCTATCAGCCGGAAAAATTATCAGGCTCTCAAGAACTCATTGAGATTATGGGACTTGGT
GCAGATGGTATTATTACAGCAGCATTTGGCCAATTTTTACCAACAATATTACTTGATTCAGTTTCATTTGCTATTAATGT
ACATGCATCATTGTTACCTAAATATCGAGGTGGAGCACCTATCCATTATGCTATTATGAATGGAGATAAAGAGGCCGGCG
TTACTATTATGGAAATGATTAAAGAAATGGATGCAGGTGACATGGTCGCTAAAGCAAGCACTCCAATTCTTGAAACCGAT
AATGTAGGTACTCTTTTTGAGAAATTAGCCATCATTGGTCGAGACTTACTTTTGGATAGTTTGCCAGCTTATCTTTCTGG
AGAATTAAAACCTATTCCTCAAGATCACAGCCAAGCTACTTTTTCACCTAATATCTCTCCAGAACATGAAAAATTAGATT
GGACAATGTCTAATCAAGAAGTGTTCAATCATATTCGAGGTATGAACCCTTGGCCGGTAGCGCATACGTTTTTAGAGGGG
CAACGTTTAAAAATTTACGAAGCTCAACTAGCTGAAGGAGAGGGATTACCTGGACAAGTTGTTGTCAAAACCAAAAAATC
TTTGGTAATAGCTACTGGACAAGGTGCTTTGTCCTTGATCGTTGTGCAACCGGCTGGTAAACCTAAAATGTCAATAATAG
ACTTTTTAAACGGCATTGGTAGAAAACTTGAAGTAGGGGACATCATTGGCAGATAA

Upstream 100 bases:

>100_bases
AAACTAATTATTGATTGTGAACCGCAACAATTTTTATAAGGTAAATCACTAAATCTAGGATGATCAGTTATGATATAATA
AAAATACAAAGAGGAAAGTC

Downstream 100 bases:

>100_bases
TTGGAAAAAAAGTACGCGAGGCAAAGCCCTCCTTGTCATTGAAGCTATTTTTGATCAGGGGGCTTACACAAACATAGCGC
TTAATCAGCAACTAAGCAAT

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MIKLLFMGTPQFSATVLKGLLDNPAYEILGVVTQPDRAVGRKKDIKVTPVKQLALEHGISIYQPEKLSGSQELIEIMGLG
ADGIITAAFGQFLPTILLDSVSFAINVHASLLPKYRGGAPIHYAIMNGDKEAGVTIMEMIKEMDAGDMVAKASTPILETD
NVGTLFEKLAIIGRDLLLDSLPAYLSGELKPIPQDHSQATFSPNISPEHEKLDWTMSNQEVFNHIRGMNPWPVAHTFLEG
QRLKIYEAQLAEGEGLPGQVVVKTKKSLVIATGQGALSLIVVQPAGKPKMSIIDFLNGIGRKLEVGDIIGR

Sequences:

>Translated_311_residues
MIKLLFMGTPQFSATVLKGLLDNPAYEILGVVTQPDRAVGRKKDIKVTPVKQLALEHGISIYQPEKLSGSQELIEIMGLG
ADGIITAAFGQFLPTILLDSVSFAINVHASLLPKYRGGAPIHYAIMNGDKEAGVTIMEMIKEMDAGDMVAKASTPILETD
NVGTLFEKLAIIGRDLLLDSLPAYLSGELKPIPQDHSQATFSPNISPEHEKLDWTMSNQEVFNHIRGMNPWPVAHTFLEG
QRLKIYEAQLAEGEGLPGQVVVKTKKSLVIATGQGALSLIVVQPAGKPKMSIIDFLNGIGRKLEVGDIIGR
>Mature_311_residues
MIKLLFMGTPQFSATVLKGLLDNPAYEILGVVTQPDRAVGRKKDIKVTPVKQLALEHGISIYQPEKLSGSQELIEIMGLG
ADGIITAAFGQFLPTILLDSVSFAINVHASLLPKYRGGAPIHYAIMNGDKEAGVTIMEMIKEMDAGDMVAKASTPILETD
NVGTLFEKLAIIGRDLLLDSLPAYLSGELKPIPQDHSQATFSPNISPEHEKLDWTMSNQEVFNHIRGMNPWPVAHTFLEG
QRLKIYEAQLAEGEGLPGQVVVKTKKSLVIATGQGALSLIVVQPAGKPKMSIIDFLNGIGRKLEVGDIIGR

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI21614513, Length=250, Percent_Identity=25.6, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI238814322, Length=265, Percent_Identity=25.6603773584906, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI164663775, Length=240, Percent_Identity=30, Blast_Score=86, Evalue=5e-17,
Organism=Escherichia coli, GI1789683, Length=299, Percent_Identity=39.4648829431438, Blast_Score=222, Evalue=2e-59,
Organism=Escherichia coli, GI1788589, Length=262, Percent_Identity=32.4427480916031, Blast_Score=127, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI133930964, Length=275, Percent_Identity=26.1818181818182, Blast_Score=96, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6319458, Length=355, Percent_Identity=25.3521126760563, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI45550868, Length=309, Percent_Identity=30.42071197411, Blast_Score=111, Evalue=6e-25,
Organism=Drosophila melanogaster, GI28571984, Length=224, Percent_Identity=32.5892857142857, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24585660, Length=264, Percent_Identity=25.7575757575758, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_STRP1 (Q99YM7)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_269678.1
- RefSeq:   YP_282701.1
- ProteinModelPortal:   Q99YM7
- SMR:   Q99YM7
- EnsemblBacteria:   EBSTRT00000000830
- EnsemblBacteria:   EBSTRT00000028256
- GeneID:   3571569
- GeneID:   901881
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_1628
- KEGG:   spz:M5005_Spy_1338
- GeneTree:   EBGT00050000028330
- HOGENOM:   HBG571560
- OMA:   VVAYGAI
- ProtClustDB:   PRK00005
- BioCyc:   SPYO160490:SPY1628-MONOMER
- BioCyc:   SPYO293653:M5005_SPY1338-MONOMER
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR001555
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 33671; Mature: 33671

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKLLFMGTPQFSATVLKGLLDNPAYEILGVVTQPDRAVGRKKDIKVTPVKQLALEHGIS
CEEEEEECCCCHHHHHHHHHHCCCCCCEEEEEECCHHHCCCCCCCEECHHHHHHHHCCCC
IYQPEKLSGSQELIEIMGLGADGIITAAFGQFLPTILLDSVSFAINVHASLLPKYRGGAP
EECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEHHHHCCCCCCCCC
IHYAIMNGDKEAGVTIMEMIKEMDAGDMVAKASTPILETDNVGTLFEKLAIIGRDLLLDS
EEEEEECCCCCCCCHHHHHHHHCCCCCEEHHCCCCCEECCCHHHHHHHHHHHHHHHHHHH
LPAYLSGELKPIPQDHSQATFSPNISPEHEKLDWTMSNQEVFNHIRGMNPWPVAHTFLEG
CCHHHCCCCCCCCCCCCCCEECCCCCCCCHHCCEEECHHHHHHHHCCCCCCCHHHHHHCC
QRLKIYEAQLAEGEGLPGQVVVKTKKSLVIATGQGALSLIVVQPAGKPKMSIIDFLNGIG
CEEEEEEEEHHCCCCCCCEEEEEECCEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHCC
RKLEVGDIIGR
CEEEHHHHCCC
>Mature Secondary Structure
MIKLLFMGTPQFSATVLKGLLDNPAYEILGVVTQPDRAVGRKKDIKVTPVKQLALEHGIS
CEEEEEECCCCHHHHHHHHHHCCCCCCEEEEEECCHHHCCCCCCCEECHHHHHHHHCCCC
IYQPEKLSGSQELIEIMGLGADGIITAAFGQFLPTILLDSVSFAINVHASLLPKYRGGAP
EECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEHHHHCCCCCCCCC
IHYAIMNGDKEAGVTIMEMIKEMDAGDMVAKASTPILETDNVGTLFEKLAIIGRDLLLDS
EEEEEECCCCCCCCHHHHHHHHCCCCCEEHHCCCCCEECCCHHHHHHHHHHHHHHHHHHH
LPAYLSGELKPIPQDHSQATFSPNISPEHEKLDWTMSNQEVFNHIRGMNPWPVAHTFLEG
CCHHHCCCCCCCCCCCCCCEECCCCCCCCHHCCEEECHHHHHHHHCCCCCCCHHHHHHCC
QRLKIYEAQLAEGEGLPGQVVVKTKKSLVIATGQGALSLIVVQPAGKPKMSIIDFLNGIG
CEEEEEEEEHHCCCCCCCEEEEEECCEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHCC
RKLEVGDIIGR
CEEEHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11296296