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The map label for this gene is queA

Identifier: 15675321

GI number: 15675321

Start: 1161365

End: 1162393

Strand: Direct

Name: queA

Synonym: SPy_1400

Alternate gene names: 15675321

Gene position: 1161365-1162393 (Clockwise)

Preceding gene: 15675309

Following gene: 15675322

Centisome position: 62.69

GC content: 42.66

Gene sequence:

>1029_bases
ATGAATACTAACAATTTTGATTTTGAGTTACCAGAAGAATTGATTGCCCAAACCCCACTCGAAAAAAGAGACAGTTCAAA
ACTATTGATTATTGATCACCGTCAAAAAACTATGGTTGATAGCCATTTTGACCATATCATTGATCAATTAAATCCAGGGG
ATGCCCTCGTCATGAATAACACCCGTGTCTTGCCTGCCAGACTTTATGGAGAAAAACCCGATACTCATGGTCATGTGGAA
TTGCTTTTGCTTAAAAATACACAAGGAGATCAATGGGAAGTTTTAGCCAAGCCAGCCAAACGGCTCAAGGTTGGTAGCCA
AGTCAATTTTGGAGATGGGCGTCTCAAAGCCACCATTATCGACGAATTAGAACATGGTGGACGAATTGTTGAATTCAGCT
ATGATGGGATTTTCTTAGAAGTTTTAGAGAGCCTCGGCGAAATGCCATTGCCTCCTTATATTCACGAAAAATTAGAGGAT
GCTGAGCGATACCAAACCGTTTATGCTAAAGAAAATGGCTCTGCGGCCGCCCCAACTGCTGGTCTCCACTTCACAACAGA
CTTGCTCAAAAAAATCGAAGCCAAAGGGGTACATTTGGTTTATTTGACCCTACATGTAGGGCTTGGGACTTTTAGACCTG
TTTCCGTGGATAACCTCGATGAGCATGATATGCATTCGGAATTTTATTCCCTCTCAGAAGAAGCAGCGCAAACCCTAAGA
GACGTCAAACAAGCTGGCGGTCGTGTTGTCGCTGTAGGAACCACCTCTATTCGTACGTTGGAAACCATCGGAGGTAAGTT
CCAAGGGGACATTCAAGCTGATTCAGGTTGGACCAATATTTTTATTAAACCAGGCTACCAATTCAAGGTAGTTGATGCTT
TTTCAACTAATTTCCATCTTCCAAAATCAACCCTTGTGATGCTAGTATCAGCCTTTGCAGGGCGTGACTTTGTCCTTGAA
GCTTACAGACACGCGGTTGACGAAAAGTATCGTTTCTTTAGCTTTGGCGATGCCATGTTTGTCAACTAA

Upstream 100 bases:

>100_bases
AAGATGACGGGTTAGACGACAAGTGATATAATAGAAAAGATTTATCTTACATGAAAGAGTTACTAGGAAGAGATTTACTA
GTGGCTTTAAAGGAGTCCCA

Downstream 100 bases:

>100_bases
AAACCTATGAAATTACTTGGAAAATGGCTATTCTTCTTGATGTTTCTGACTTTTTGCGTCAGTTTTTGGCAGAAAGACTA
TCAGCGGATTGCCTTTGAAA

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MNTNNFDFELPEELIAQTPLEKRDSSKLLIIDHRQKTMVDSHFDHIIDQLNPGDALVMNNTRVLPARLYGEKPDTHGHVE
LLLLKNTQGDQWEVLAKPAKRLKVGSQVNFGDGRLKATIIDELEHGGRIVEFSYDGIFLEVLESLGEMPLPPYIHEKLED
AERYQTVYAKENGSAAAPTAGLHFTTDLLKKIEAKGVHLVYLTLHVGLGTFRPVSVDNLDEHDMHSEFYSLSEEAAQTLR
DVKQAGGRVVAVGTTSIRTLETIGGKFQGDIQADSGWTNIFIKPGYQFKVVDAFSTNFHLPKSTLVMLVSAFAGRDFVLE
AYRHAVDEKYRFFSFGDAMFVN

Sequences:

>Translated_342_residues
MNTNNFDFELPEELIAQTPLEKRDSSKLLIIDHRQKTMVDSHFDHIIDQLNPGDALVMNNTRVLPARLYGEKPDTHGHVE
LLLLKNTQGDQWEVLAKPAKRLKVGSQVNFGDGRLKATIIDELEHGGRIVEFSYDGIFLEVLESLGEMPLPPYIHEKLED
AERYQTVYAKENGSAAAPTAGLHFTTDLLKKIEAKGVHLVYLTLHVGLGTFRPVSVDNLDEHDMHSEFYSLSEEAAQTLR
DVKQAGGRVVAVGTTSIRTLETIGGKFQGDIQADSGWTNIFIKPGYQFKVVDAFSTNFHLPKSTLVMLVSAFAGRDFVLE
AYRHAVDEKYRFFSFGDAMFVN
>Mature_342_residues
MNTNNFDFELPEELIAQTPLEKRDSSKLLIIDHRQKTMVDSHFDHIIDQLNPGDALVMNNTRVLPARLYGEKPDTHGHVE
LLLLKNTQGDQWEVLAKPAKRLKVGSQVNFGDGRLKATIIDELEHGGRIVEFSYDGIFLEVLESLGEMPLPPYIHEKLED
AERYQTVYAKENGSAAAPTAGLHFTTDLLKKIEAKGVHLVYLTLHVGLGTFRPVSVDNLDEHDMHSEFYSLSEEAAQTLR
DVKQAGGRVVAVGTTSIRTLETIGGKFQGDIQADSGWTNIFIKPGYQFKVVDAFSTNFHLPKSTLVMLVSAFAGRDFVLE
AYRHAVDEKYRFFSFGDAMFVN

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=347, Percent_Identity=47.5504322766571, Blast_Score=310, Evalue=7e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_STRP1 (Q99Z49)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_269495.1
- RefSeq:   YP_282503.1
- ProteinModelPortal:   Q99Z49
- SMR:   Q99Z49
- EnsemblBacteria:   EBSTRT00000000834
- EnsemblBacteria:   EBSTRT00000027614
- GeneID:   3571764
- GeneID:   901464
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_1400
- KEGG:   spz:M5005_Spy_1140
- GeneTree:   EBGT00050000028225
- HOGENOM:   HBG286076
- OMA:   SEFYRLT
- ProtClustDB:   PRK00147
- BioCyc:   SPYO160490:SPY1400-MONOMER
- BioCyc:   SPYO293653:M5005_SPY1140-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38352; Mature: 38352

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTNNFDFELPEELIAQTPLEKRDSSKLLIIDHRQKTMVDSHFDHIIDQLNPGDALVMNN
CCCCCCCCCCCHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCEEEECC
TRVLPARLYGEKPDTHGHVELLLLKNTQGDQWEVLAKPAKRLKVGSQVNFGDGRLKATII
CEEEEHHHCCCCCCCCCCEEEEEEECCCCCCHHHHHCCHHHHCCCCEEECCCCEEEEEEE
DELEHGGRIVEFSYDGIFLEVLESLGEMPLPPYIHEKLEDAERYQTVYAKENGSAAAPTA
HHHHCCCEEEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEEEECCCCCCCCCC
GLHFTTDLLKKIEAKGVHLVYLTLHVGLGTFRPVSVDNLDEHDMHSEFYSLSEEAAQTLR
CCCHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
DVKQAGGRVVAVGTTSIRTLETIGGKFQGDIQADSGWTNIFIKPGYQFKVVDAFSTNFHL
HHHHCCCEEEEEECCCHHHHHHHCCCEECCEECCCCCEEEEECCCCEEEEEEECCCCCCC
PKSTLVMLVSAFAGRDFVLEAYRHAVDEKYRFFSFGDAMFVN
CHHHHHHHHHHHCCCHHHHHHHHHHHHHCEEEEEECCEEEEC
>Mature Secondary Structure
MNTNNFDFELPEELIAQTPLEKRDSSKLLIIDHRQKTMVDSHFDHIIDQLNPGDALVMNN
CCCCCCCCCCCHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCEEEECC
TRVLPARLYGEKPDTHGHVELLLLKNTQGDQWEVLAKPAKRLKVGSQVNFGDGRLKATII
CEEEEHHHCCCCCCCCCCEEEEEEECCCCCCHHHHHCCHHHHCCCCEEECCCCEEEEEEE
DELEHGGRIVEFSYDGIFLEVLESLGEMPLPPYIHEKLEDAERYQTVYAKENGSAAAPTA
HHHHCCCEEEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEEEECCCCCCCCCC
GLHFTTDLLKKIEAKGVHLVYLTLHVGLGTFRPVSVDNLDEHDMHSEFYSLSEEAAQTLR
CCCHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
DVKQAGGRVVAVGTTSIRTLETIGGKFQGDIQADSGWTNIFIKPGYQFKVVDAFSTNFHL
HHHHCCCEEEEEECCCHHHHHHHCCCEECCEECCCCCEEEEECCCCEEEEEEECCCCCCC
PKSTLVMLVSAFAGRDFVLEAYRHAVDEKYRFFSFGDAMFVN
CHHHHHHHHHHHCCCHHHHHHHHHHHHHCEEEEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11296296