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The map label for this gene is pyk [H]

Identifier: 15675235

GI number: 15675235

Start: 1056379

End: 1057881

Strand: Reverse

Name: pyk [H]

Synonym: SPy_1282

Alternate gene names: 15675235

Gene position: 1057881-1056379 (Counterclockwise)

Preceding gene: 15675236

Following gene: 15675234

Centisome position: 57.11

GC content: 40.45

Gene sequence:

>1503_bases
ATGAATAAACGCGTAAAAATCGTTGCAACACTTGGTCCTGCGGTTGAAATCCGTGGTGGTAAAAAATACGGAGAAGATGG
CTACTGGGCTGGTCAACTTGATGTTGAAGAATCAGCAAAAAAAATCGCTGAATTAATTGAAGCTGGTGCGAATGTTTTCC
GTTTCAATTTCTCACACGGTGACCACAAAGAGCAAGGTGACCGTATGGCAACTGTTCGCCTTGCAGAAGAAATTGCTCGT
CAAAAAGTTGGTTTCCTTTTGGATACTAAAGGTCCTGAAATGCGTACGGAATTGTTTGCAGATGATGCTAAAGAGTTTAG
CTATGTTACTGGAGAAAAAATCCGTGTAGCGACTACACAAGGTATTCAATCAACTCGCGATGTTATTGCTTTGAACGTTG
CTGGTAGCCTTGACATCTACGATGAAGTAGAAGTAGGTCATACTATTCTTATCGATGATGGTAAACTTGGTTTGAAAGTG
ATCGACAAAGATATCGCAACACGTCAATTTATCGTTGAAGTAGAAAACGACGGTATTATTGCTAAACAAAAAGGTGTTAA
TATCCCTAACACTAAAATTCCTTTCCCAGCACTTGCGGAACGCGACAATGCTGATATCCGTTTTGGTCTTGAACAAGGAC
TTAACTTTATTGCAATCTCATTTGTTCGTACAGCCAAAGACGTTGAGGAAGTTCGTGAAATCTGTCGCGAAACTGGTAAC
GACCATGTGCAATTATTTGCTAAGATTGAAAACCAACAAGGTATCGATAATCTTGATGAAATCATCGAAGCTGCTGATGG
TATCATGATTGCACGTGGCGACATGGGTATCGAAGTACCATTTGAAATGGTTCCAGTTTTCCAAAAAATGATTATCACTA
AAGTGAATGCAGCTGGTAAAGCAGTTATCACAGCAACAAACATGCTTGAAACAATGACTGAAAAACCACGTGCGACACGT
TCTGAAGTATCTGACGTCTTCAATGCTGTTATCGATGGTACTGATGCAACAATGCTTTCAGGAGAGTCAGCTAACGGTAA
ATACCCGGTTGAATCTGTTCGTACAATGGCGACTATTGATCGTAATGCACAAACTCTTCTTAACGAATATGGTCGTTTAG
ATTCATCTGCCTTCCCACGTACCAATAAAACAGATGTTATTGCTTCAGCTGTTAAAGATGCCACTCATTCAATGGACATC
AAACTTGTTGTGACTATTACTGAGACAGGTAATACAGCTCGTGCGATTTCTAAATTCCGTCCAGATGCGGACATCTTAGC
TGTAACATTTGATGAAAAAGTACAACGTGCTTTGATGATTAACTGGGGTGTTATTCCTGTTCTTGCTGAAAAACCTGCAT
CTACTGATGACATGTTTGAAGTAGCAGAGCGTGTTGCTGTGGAGGCAGGCTTAGTTCAATCTGGGGATAATATTGTTATT
GTTGCTGGTGTTCCAGTTGGTACTGGTGGAACGAATACTATGCGTGTTCGTACAGTAAAATAA

Upstream 100 bases:

>100_bases
TAGACTTTGCAGCTTTGAACCGTTCATTGTCACAATAAGTTTAGTTAAAATTGTTAAATCGTCAATTATTGACAAAAAAA
TAAGGGAGTTTCAGAGTAAA

Downstream 100 bases:

>100_bases
ATGTGTTATAACTATTTACCTAAAGGTTAGAAGGCAATATTCTAACCTTTTTTGATATCAAAAATATCATGTTAATTCAC
TACTGTTAATTTACTTTAAT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MNKRVKIVATLGPAVEIRGGKKYGEDGYWAGQLDVEESAKKIAELIEAGANVFRFNFSHGDHKEQGDRMATVRLAEEIAR
QKVGFLLDTKGPEMRTELFADDAKEFSYVTGEKIRVATTQGIQSTRDVIALNVAGSLDIYDEVEVGHTILIDDGKLGLKV
IDKDIATRQFIVEVENDGIIAKQKGVNIPNTKIPFPALAERDNADIRFGLEQGLNFIAISFVRTAKDVEEVREICRETGN
DHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMGIEVPFEMVPVFQKMIITKVNAAGKAVITATNMLETMTEKPRATR
SEVSDVFNAVIDGTDATMLSGESANGKYPVESVRTMATIDRNAQTLLNEYGRLDSSAFPRTNKTDVIASAVKDATHSMDI
KLVVTITETGNTARAISKFRPDADILAVTFDEKVQRALMINWGVIPVLAEKPASTDDMFEVAERVAVEAGLVQSGDNIVI
VAGVPVGTGGTNTMRVRTVK

Sequences:

>Translated_500_residues
MNKRVKIVATLGPAVEIRGGKKYGEDGYWAGQLDVEESAKKIAELIEAGANVFRFNFSHGDHKEQGDRMATVRLAEEIAR
QKVGFLLDTKGPEMRTELFADDAKEFSYVTGEKIRVATTQGIQSTRDVIALNVAGSLDIYDEVEVGHTILIDDGKLGLKV
IDKDIATRQFIVEVENDGIIAKQKGVNIPNTKIPFPALAERDNADIRFGLEQGLNFIAISFVRTAKDVEEVREICRETGN
DHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMGIEVPFEMVPVFQKMIITKVNAAGKAVITATNMLETMTEKPRATR
SEVSDVFNAVIDGTDATMLSGESANGKYPVESVRTMATIDRNAQTLLNEYGRLDSSAFPRTNKTDVIASAVKDATHSMDI
KLVVTITETGNTARAISKFRPDADILAVTFDEKVQRALMINWGVIPVLAEKPASTDDMFEVAERVAVEAGLVQSGDNIVI
VAGVPVGTGGTNTMRVRTVK
>Mature_500_residues
MNKRVKIVATLGPAVEIRGGKKYGEDGYWAGQLDVEESAKKIAELIEAGANVFRFNFSHGDHKEQGDRMATVRLAEEIAR
QKVGFLLDTKGPEMRTELFADDAKEFSYVTGEKIRVATTQGIQSTRDVIALNVAGSLDIYDEVEVGHTILIDDGKLGLKV
IDKDIATRQFIVEVENDGIIAKQKGVNIPNTKIPFPALAERDNADIRFGLEQGLNFIAISFVRTAKDVEEVREICRETGN
DHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMGIEVPFEMVPVFQKMIITKVNAAGKAVITATNMLETMTEKPRATR
SEVSDVFNAVIDGTDATMLSGESANGKYPVESVRTMATIDRNAQTLLNEYGRLDSSAFPRTNKTDVIASAVKDATHSMDI
KLVVTITETGNTARAISKFRPDADILAVTFDEKVQRALMINWGVIPVLAEKPASTDDMFEVAERVAVEAGLVQSGDNIVI
VAGVPVGTGGTNTMRVRTVK

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=483, Percent_Identity=38.3022774327122, Blast_Score=315, Evalue=8e-86,
Organism=Homo sapiens, GI33286420, Length=483, Percent_Identity=38.3022774327122, Blast_Score=315, Evalue=8e-86,
Organism=Homo sapiens, GI33286418, Length=483, Percent_Identity=38.3022774327122, Blast_Score=312, Evalue=6e-85,
Organism=Homo sapiens, GI10835121, Length=513, Percent_Identity=36.2573099415205, Blast_Score=310, Evalue=2e-84,
Organism=Homo sapiens, GI32967597, Length=514, Percent_Identity=36.1867704280156, Blast_Score=310, Evalue=2e-84,
Organism=Homo sapiens, GI310128732, Length=279, Percent_Identity=39.7849462365591, Blast_Score=189, Evalue=4e-48,
Organism=Homo sapiens, GI310128730, Length=279, Percent_Identity=39.7849462365591, Blast_Score=189, Evalue=4e-48,
Organism=Homo sapiens, GI310128736, Length=258, Percent_Identity=32.9457364341085, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI310128734, Length=258, Percent_Identity=32.9457364341085, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI310128738, Length=220, Percent_Identity=35.4545454545455, Blast_Score=130, Evalue=4e-30,
Organism=Escherichia coli, GI1787965, Length=501, Percent_Identity=40.1197604790419, Blast_Score=370, Evalue=1e-103,
Organism=Escherichia coli, GI1788160, Length=512, Percent_Identity=33.203125, Blast_Score=222, Evalue=5e-59,
Organism=Caenorhabditis elegans, GI17544584, Length=510, Percent_Identity=38.0392156862745, Blast_Score=328, Evalue=3e-90,
Organism=Caenorhabditis elegans, GI71984413, Length=513, Percent_Identity=38.2066276803119, Blast_Score=300, Evalue=1e-81,
Organism=Caenorhabditis elegans, GI71984406, Length=513, Percent_Identity=38.2066276803119, Blast_Score=300, Evalue=1e-81,
Organism=Caenorhabditis elegans, GI17506829, Length=513, Percent_Identity=38.2066276803119, Blast_Score=300, Evalue=1e-81,
Organism=Caenorhabditis elegans, GI17506831, Length=511, Percent_Identity=38.3561643835616, Blast_Score=300, Evalue=1e-81,
Organism=Saccharomyces cerevisiae, GI6319279, Length=468, Percent_Identity=37.8205128205128, Blast_Score=298, Evalue=2e-81,
Organism=Saccharomyces cerevisiae, GI6324923, Length=509, Percent_Identity=36.7387033398821, Blast_Score=291, Evalue=2e-79,
Organism=Drosophila melanogaster, GI24648964, Length=519, Percent_Identity=36.8015414258189, Blast_Score=315, Evalue=4e-86,
Organism=Drosophila melanogaster, GI28571814, Length=519, Percent_Identity=36.8015414258189, Blast_Score=315, Evalue=7e-86,
Organism=Drosophila melanogaster, GI24648966, Length=414, Percent_Identity=37.9227053140097, Blast_Score=265, Evalue=5e-71,
Organism=Drosophila melanogaster, GI24581235, Length=474, Percent_Identity=32.0675105485232, Blast_Score=244, Evalue=1e-64,
Organism=Drosophila melanogaster, GI24646914, Length=348, Percent_Identity=32.4712643678161, Blast_Score=175, Evalue=6e-44,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 54536; Mature: 54536

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKRVKIVATLGPAVEIRGGKKYGEDGYWAGQLDVEESAKKIAELIEAGANVFRFNFSHG
CCCEEEEEEEECCEEEECCCCCCCCCCEEEEEECHHHHHHHHHHHHHCCCCEEEEECCCC
DHKEQGDRMATVRLAEEIARQKVGFLLDTKGPEMRTELFADDAKEFSYVTGEKIRVATTQ
CCCCCCCHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHCCHHHHHEECCCEEEEEECC
GIQSTRDVIALNVAGSLDIYDEVEVGHTILIDDGKLGLKVIDKDIATRQFIVEVENDGII
CCCCCCCEEEEEECCCCCCCEECCCCCEEEEECCCCCEEEECCHHCCEEEEEEECCCCEE
AKQKGVNIPNTKIPFPALAERDNADIRFGLEQGLNFIAISFVRTAKDVEEVREICRETGN
EECCCCCCCCCCCCCCCCCCCCCCCEEEEHHHCCCEEEHHHHHHHHHHHHHHHHHHHCCC
DHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMGIEVPFEMVPVFQKMIITKVNAAGK
CEEEEEEEECCCCCCCHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCC
AVITATNMLETMTEKPRATRSEVSDVFNAVIDGTDATMLSGESANGKYPVESVRTMATID
EEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHC
RNAQTLLNEYGRLDSSAFPRTNKTDVIASAVKDATHSMDIKLVVTITETGNTARAISKFR
CCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHC
PDADILAVTFDEKVQRALMINWGVIPVLAEKPASTDDMFEVAERVAVEAGLVQSGDNIVI
CCCCEEEEEECHHHHHHEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHEECCCCEEE
VAGVPVGTGGTNTMRVRTVK
EEECCCCCCCCCEEEEEEEC
>Mature Secondary Structure
MNKRVKIVATLGPAVEIRGGKKYGEDGYWAGQLDVEESAKKIAELIEAGANVFRFNFSHG
CCCEEEEEEEECCEEEECCCCCCCCCCEEEEEECHHHHHHHHHHHHHCCCCEEEEECCCC
DHKEQGDRMATVRLAEEIARQKVGFLLDTKGPEMRTELFADDAKEFSYVTGEKIRVATTQ
CCCCCCCHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHCCHHHHHEECCCEEEEEECC
GIQSTRDVIALNVAGSLDIYDEVEVGHTILIDDGKLGLKVIDKDIATRQFIVEVENDGII
CCCCCCCEEEEEECCCCCCCEECCCCCEEEEECCCCCEEEECCHHCCEEEEEEECCCCEE
AKQKGVNIPNTKIPFPALAERDNADIRFGLEQGLNFIAISFVRTAKDVEEVREICRETGN
EECCCCCCCCCCCCCCCCCCCCCCCEEEEHHHCCCEEEHHHHHHHHHHHHHHHHHHHCCC
DHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMGIEVPFEMVPVFQKMIITKVNAAGK
CEEEEEEEECCCCCCCHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCC
AVITATNMLETMTEKPRATRSEVSDVFNAVIDGTDATMLSGESANGKYPVESVRTMATID
EEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHC
RNAQTLLNEYGRLDSSAFPRTNKTDVIASAVKDATHSMDIKLVVTITETGNTARAISKFR
CCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHC
PDADILAVTFDEKVQRALMINWGVIPVLAEKPASTDDMFEVAERVAVEAGLVQSGDNIVI
CCCCEEEEEECHHHHHHEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHEECCCCEEE
VAGVPVGTGGTNTMRVRTVK
EEECCCCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8478320; 11337471 [H]