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The map label for this gene is pfkA [H]

Identifier: 15675236

GI number: 15675236

Start: 1057944

End: 1058957

Strand: Reverse

Name: pfkA [H]

Synonym: SPy_1283

Alternate gene names: 15675236

Gene position: 1058957-1057944 (Counterclockwise)

Preceding gene: 15675237

Following gene: 15675235

Centisome position: 57.17

GC content: 43.29

Gene sequence:

>1014_bases
ATGAAACGTATTGCTGTTTTAACCAGTGGCGGCGACGCTCCTGGTATGAACGCTGCTATTCGTGCAGTCGTTCGTAAAGC
AATTTCAGAAGGAATGGAAGTTTATGGTATCAACCGTGGCTATGCAGGTATGGTTGATGGCGACATTTTCCCACTGGGTT
CAAAAGAAGTTGGTGATAAAATCTCACGTGGTGGAACTTTCTTATACTCAGCTCGTTATCCAGAGTTTGCTCAACTTGAA
GGTCAATTGGCTGGTATTGAGCAACTTAAAAAACATGGTATTGAAGGTGTTGTTGTTATTGGTGGGGATGGTTCTTATCA
TGGCGCTATGCGCTTGACAGAGCACGGCTTCCCAGCGGTAGGTATCCCAGGAACAATCGATAACGATATTGCGGGAACTG
ACTACACTATCGGATTTGATACAGCAGTTAACACAGCAGTTGAAGCTATTGATAAACTTCGTGACACCTCATCAAGTCAC
GGACGTACTTTTGTGGTTGAAGTGATGGGTCGCAATGCGGGTGATATTGCTCTTTGGGCAGGTATTGCTAGTGGTGCTGA
TCAAATCATTGTTCCAGAAGAAGAGTTTGATATTGAAAAAGTGGCATCAACAATCCAATATGATTTTGAACACAAAGGTA
AAAATCACCACATTATCGTCTTAGCTGAAGGTGTTATGAGCGGTGAAGCTTTTGCTCAAAAACTGAAAGAAGCTGGCGAT
AAGAGTGACCTTCGTGTGACAAATCTTGGCCACATTCTTCGTGGGGGTTCACCAACTGCTCGTGACCGTGTCATTGCTTC
TTGGATGGGTTCACATGCTGTTGAGCTACTTAAAGATGGCAAAGGTGGTTTGGCTGTTGGTATTCACAATGAAGAACTTG
TTGAAAGCCCAATCCTTGGTACTGCAGAAGAAGGTGCTTTGTTCAGCTTGACTGAAGAAGGCAAGATTATTGTTAATAAT
CCACACAAAGCTCGTTTAGACTTTGCAGCTTTGAACCGTTCATTGTCACAATAA

Upstream 100 bases:

>100_bases
CTGAAAACGGTTTTTCGATAAAAATGGACAATTAGTGAAGTATTTTGCAAATGAATGTGCTATAATAAGACCGTTAAATT
AATTAAAGGAGTACCAGAAA

Downstream 100 bases:

>100_bases
GTTTAGTTAAAATTGTTAAATCGTCAATTATTGACAAAAAAATAAGGGAGTTTCAGAGTAAAATGAATAAACGCGTAAAA
ATCGTTGCAACACTTGGTCC

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MKRIAVLTSGGDAPGMNAAIRAVVRKAISEGMEVYGINRGYAGMVDGDIFPLGSKEVGDKISRGGTFLYSARYPEFAQLE
GQLAGIEQLKKHGIEGVVVIGGDGSYHGAMRLTEHGFPAVGIPGTIDNDIAGTDYTIGFDTAVNTAVEAIDKLRDTSSSH
GRTFVVEVMGRNAGDIALWAGIASGADQIIVPEEEFDIEKVASTIQYDFEHKGKNHHIIVLAEGVMSGEAFAQKLKEAGD
KSDLRVTNLGHILRGGSPTARDRVIASWMGSHAVELLKDGKGGLAVGIHNEELVESPILGTAEEGALFSLTEEGKIIVNN
PHKARLDFAALNRSLSQ

Sequences:

>Translated_337_residues
MKRIAVLTSGGDAPGMNAAIRAVVRKAISEGMEVYGINRGYAGMVDGDIFPLGSKEVGDKISRGGTFLYSARYPEFAQLE
GQLAGIEQLKKHGIEGVVVIGGDGSYHGAMRLTEHGFPAVGIPGTIDNDIAGTDYTIGFDTAVNTAVEAIDKLRDTSSSH
GRTFVVEVMGRNAGDIALWAGIASGADQIIVPEEEFDIEKVASTIQYDFEHKGKNHHIIVLAEGVMSGEAFAQKLKEAGD
KSDLRVTNLGHILRGGSPTARDRVIASWMGSHAVELLKDGKGGLAVGIHNEELVESPILGTAEEGALFSLTEEGKIIVNN
PHKARLDFAALNRSLSQ
>Mature_337_residues
MKRIAVLTSGGDAPGMNAAIRAVVRKAISEGMEVYGINRGYAGMVDGDIFPLGSKEVGDKISRGGTFLYSARYPEFAQLE
GQLAGIEQLKKHGIEGVVVIGGDGSYHGAMRLTEHGFPAVGIPGTIDNDIAGTDYTIGFDTAVNTAVEAIDKLRDTSSSH
GRTFVVEVMGRNAGDIALWAGIASGADQIIVPEEEFDIEKVASTIQYDFEHKGKNHHIIVLAEGVMSGEAFAQKLKEAGD
KSDLRVTNLGHILRGGSPTARDRVIASWMGSHAVELLKDGKGGLAVGIHNEELVESPILGTAEEGALFSLTEEGKIIVNN
PHKARLDFAALNRSLSQ

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI11321601, Length=307, Percent_Identity=42.0195439739414, Blast_Score=191, Evalue=7e-49,
Organism=Homo sapiens, GI48762920, Length=315, Percent_Identity=40, Blast_Score=189, Evalue=3e-48,
Organism=Homo sapiens, GI266453768, Length=340, Percent_Identity=37.9411764705882, Blast_Score=185, Evalue=4e-47,
Organism=Homo sapiens, GI266453748, Length=340, Percent_Identity=37.9411764705882, Blast_Score=185, Evalue=4e-47,
Organism=Homo sapiens, GI4505749, Length=340, Percent_Identity=37.9411764705882, Blast_Score=185, Evalue=4e-47,
Organism=Homo sapiens, GI266453619, Length=340, Percent_Identity=37.3529411764706, Blast_Score=185, Evalue=5e-47,
Organism=Escherichia coli, GI1790350, Length=304, Percent_Identity=55.921052631579, Blast_Score=315, Evalue=4e-87,
Organism=Caenorhabditis elegans, GI25147584, Length=343, Percent_Identity=37.0262390670554, Blast_Score=182, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17558788, Length=310, Percent_Identity=35.8064516129032, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI72003601, Length=288, Percent_Identity=31.9444444444444, Blast_Score=147, Evalue=8e-36,
Organism=Saccharomyces cerevisiae, GI6321679, Length=336, Percent_Identity=36.6071428571429, Blast_Score=185, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6323861, Length=338, Percent_Identity=36.094674556213, Blast_Score=178, Evalue=1e-45,
Organism=Drosophila melanogaster, GI28573326, Length=339, Percent_Identity=38.6430678466077, Blast_Score=200, Evalue=1e-51,
Organism=Drosophila melanogaster, GI24652337, Length=339, Percent_Identity=38.9380530973451, Blast_Score=199, Evalue=2e-51,
Organism=Drosophila melanogaster, GI17647809, Length=340, Percent_Identity=38.8235294117647, Blast_Score=199, Evalue=2e-51,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 35749; Mature: 35749

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRIAVLTSGGDAPGMNAAIRAVVRKAISEGMEVYGINRGYAGMVDGDIFPLGSKEVGDK
CCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCEEECCCHHHHHH
ISRGGTFLYSARYPEFAQLEGQLAGIEQLKKHGIEGVVVIGGDGSYHGAMRLTEHGFPAV
HHCCCEEEEECCCCCHHHHCCHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEECCCCCCC
GIPGTIDNDIAGTDYTIGFDTAVNTAVEAIDKLRDTSSSHGRTFVVEVMGRNAGDIALWA
CCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCEEEEE
GIASGADQIIVPEEEFDIEKVASTIQYDFEHKGKNHHIIVLAEGVMSGEAFAQKLKEAGD
ECCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHCCC
KSDLRVTNLGHILRGGSPTARDRVIASWMGSHAVELLKDGKGGLAVGIHNEELVESPILG
CCCEEEECCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHCCCCC
TAEEGALFSLTEEGKIIVNNPHKARLDFAALNRSLSQ
CCCCCCEEEEECCCEEEEECCCHHEEHHHHHHHHHCC
>Mature Secondary Structure
MKRIAVLTSGGDAPGMNAAIRAVVRKAISEGMEVYGINRGYAGMVDGDIFPLGSKEVGDK
CCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCEEECCCHHHHHH
ISRGGTFLYSARYPEFAQLEGQLAGIEQLKKHGIEGVVVIGGDGSYHGAMRLTEHGFPAV
HHCCCEEEEECCCCCHHHHCCHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEECCCCCCC
GIPGTIDNDIAGTDYTIGFDTAVNTAVEAIDKLRDTSSSHGRTFVVEVMGRNAGDIALWA
CCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCEEEEE
GIASGADQIIVPEEEFDIEKVASTIQYDFEHKGKNHHIIVLAEGVMSGEAFAQKLKEAGD
ECCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHCCC
KSDLRVTNLGHILRGGSPTARDRVIASWMGSHAVELLKDGKGGLAVGIHNEELVESPILG
CCCEEEECCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHCCCCC
TAEEGALFSLTEEGKIIVNNPHKARLDFAALNRSLSQ
CCCCCCEEEEECCCEEEEECCCHHEEHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA