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The map label for this gene is bcaT [H]

Identifier: 15674932

GI number: 15674932

Start: 756974

End: 757996

Strand: Direct

Name: bcaT [H]

Synonym: SPy_0911

Alternate gene names: 15674932

Gene position: 756974-757996 (Clockwise)

Preceding gene: 15674931

Following gene: 15674933

Centisome position: 40.86

GC content: 40.86

Gene sequence:

>1023_bases
ATGACAATAGCAATTGATTGGGACAACCTAGGATTTGAATATCATAAGTTGCCATTTCGTTACATTTCTTATTACAAAAA
TGGTCAATGGGATAAAGGTCAATTAACAGAAGATGCCACCTTACATATCTCAGAAAGCGCTCCAGCGCTTCATTATGGAC
AACAAGCTTTTGAAGGCTTAAAGGCTTATCGCACCAAAGATGGCTCTATTCAACTATTTAGACCAGACCGTAATGCAGTG
CGTTTACAAGCCACAGCAGACCGCTTGTTGATGCCACAAGTATCAACAGAGCAATTTATTGATGCAGCTAAGCAAGTGGT
TAAAGCCAATGAAGACTTTGTACCACCTTACGGAACAGGTGCAACGCTTTACCTGAGACCGTTATTAATTGGAGTTGGAG
ATATTATTGGGGTTAAACCAGCCGAAGAATATATTTTCACCATTTTTGCCATGCCAGTAGGAAACTATTTCAAAGGTGGA
CTTGCTCCAACTAATTTTATTGTTTCTGAAGCGTTTGACCGCGCCGCTCCTTACGGAACAGGTGCTGCTAAGGTGGGAGG
AAACTATGCAGGAAGTTTGCTACCAGGTAAGGCCGCTAAATCAGCAGGCTTTTCTGATGTGATTTACTTAGATCCAGCAA
CCCATACTAAAATTGAAGAAGTCGGAGCTGCAAACTTTTTTGGCATTACTGCAAATAATGAATTTGTTACGCCACTTAGT
CCATCCATCTTGCCATCTATTACGAAGTATTCACTCTTACAACTAGCTGAAGAAAGACTTGGTATGACAGTAATTGAGGG
TGATGTTCCAATTAATGAATTGGATAAATTTGTGGAAGCAGGAGCTTGTGGAACAGCAGCTGTTATCTCTCCAATTGGCG
GTATCCAGTACAAGGATAACTTACATGTCTTTTACAGTGAAACAGAAGTTGGTCCTGTGACACGCCGTTTATACGATGAG
TTGGTCGGCATTCAATTTGGGGATATTGAAGCACCAGAAGGTTGGATTGTAAAAGTAGATTAA

Upstream 100 bases:

>100_bases
TTTTATGATAAATTTAGAATATTAATACAAAAGAATAGAATAAACTTGAAAATTATTGAAAAAAAGTTACAATAGAAAAA
AGCAGAAAAGAGGTACGATG

Downstream 100 bases:

>100_bases
AATAGAGCTTGCTCTTGCAAGCTTTTTTGATTTTGTGTAAAATAGACAAAGTGAGGTAAAGTGATGAGTAAAAAAGATAA
AAAAATTGAAATCCAACTGA

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MTIAIDWDNLGFEYHKLPFRYISYYKNGQWDKGQLTEDATLHISESAPALHYGQQAFEGLKAYRTKDGSIQLFRPDRNAV
RLQATADRLLMPQVSTEQFIDAAKQVVKANEDFVPPYGTGATLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
LAPTNFIVSEAFDRAAPYGTGAAKVGGNYAGSLLPGKAAKSAGFSDVIYLDPATHTKIEEVGAANFFGITANNEFVTPLS
PSILPSITKYSLLQLAEERLGMTVIEGDVPINELDKFVEAGACGTAAVISPIGGIQYKDNLHVFYSETEVGPVTRRLYDE
LVGIQFGDIEAPEGWIVKVD

Sequences:

>Translated_340_residues
MTIAIDWDNLGFEYHKLPFRYISYYKNGQWDKGQLTEDATLHISESAPALHYGQQAFEGLKAYRTKDGSIQLFRPDRNAV
RLQATADRLLMPQVSTEQFIDAAKQVVKANEDFVPPYGTGATLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
LAPTNFIVSEAFDRAAPYGTGAAKVGGNYAGSLLPGKAAKSAGFSDVIYLDPATHTKIEEVGAANFFGITANNEFVTPLS
PSILPSITKYSLLQLAEERLGMTVIEGDVPINELDKFVEAGACGTAAVISPIGGIQYKDNLHVFYSETEVGPVTRRLYDE
LVGIQFGDIEAPEGWIVKVD
>Mature_339_residues
TIAIDWDNLGFEYHKLPFRYISYYKNGQWDKGQLTEDATLHISESAPALHYGQQAFEGLKAYRTKDGSIQLFRPDRNAVR
LQATADRLLMPQVSTEQFIDAAKQVVKANEDFVPPYGTGATLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGGL
APTNFIVSEAFDRAAPYGTGAAKVGGNYAGSLLPGKAAKSAGFSDVIYLDPATHTKIEEVGAANFFGITANNEFVTPLSP
SILPSITKYSLLQLAEERLGMTVIEGDVPINELDKFVEAGACGTAAVISPIGGIQYKDNLHVFYSETEVGPVTRRLYDEL
VGIQFGDIEAPEGWIVKVD

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI38176287, Length=334, Percent_Identity=32.9341317365269, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI50658084, Length=318, Percent_Identity=35.5345911949685, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI296010906, Length=334, Percent_Identity=32.9341317365269, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI296010904, Length=334, Percent_Identity=32.9341317365269, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI258614015, Length=300, Percent_Identity=35.3333333333333, Blast_Score=148, Evalue=7e-36,
Organism=Homo sapiens, GI296010902, Length=249, Percent_Identity=32.9317269076305, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI296010900, Length=249, Percent_Identity=32.9317269076305, Blast_Score=113, Evalue=3e-25,
Organism=Escherichia coli, GI48994963, Length=320, Percent_Identity=32.8125, Blast_Score=123, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17568601, Length=325, Percent_Identity=32.9230769230769, Blast_Score=166, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17565728, Length=353, Percent_Identity=30.3116147308782, Blast_Score=120, Evalue=8e-28,
Organism=Saccharomyces cerevisiae, GI6322608, Length=319, Percent_Identity=33.8557993730408, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6322002, Length=317, Percent_Identity=32.1766561514196, Blast_Score=158, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24641779, Length=333, Percent_Identity=33.033033033033, Blast_Score=168, Evalue=6e-42,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 37063; Mature: 36932

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIAIDWDNLGFEYHKLPFRYISYYKNGQWDKGQLTEDATLHISESAPALHYGQQAFEGL
CEEEEECCCCCCCHHCCHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHH
KAYRTKDGSIQLFRPDRNAVRLQATADRLLMPQVSTEQFIDAAKQVVKANEDFVPPYGTG
HHHCCCCCCEEEECCCCCEEEEEECCCHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
ATLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGGLAPTNFIVSEAFDRAAPYGT
CCEEEHHHHHHHHHHCCCCCHHHHEEEEEEECCCCHHCCCCCCHHHHHHHHHHHCCCCCC
GAAKVGGNYAGSLLPGKAAKSAGFSDVIYLDPATHTKIEEVGAANFFGITANNEFVTPLS
CHHHCCCCCCCCCCCCCHHCCCCCCEEEEECCCCCCHHHHCCCCEEEEEECCCCEECCCC
PSILPSITKYSLLQLAEERLGMTVIEGDVPINELDKFVEAGACGTAAVISPIGGIQYKDN
CHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHCCCCCCHHHHHCCCCCEECCC
LHVFYSETEVGPVTRRLYDELVGIQFGDIEAPEGWIVKVD
EEEEEECCCCCHHHHHHHHHHHCCEECCCCCCCCEEEEEC
>Mature Secondary Structure 
TIAIDWDNLGFEYHKLPFRYISYYKNGQWDKGQLTEDATLHISESAPALHYGQQAFEGL
EEEEECCCCCCCHHCCHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHH
KAYRTKDGSIQLFRPDRNAVRLQATADRLLMPQVSTEQFIDAAKQVVKANEDFVPPYGTG
HHHCCCCCCEEEECCCCCEEEEEECCCHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
ATLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGGLAPTNFIVSEAFDRAAPYGT
CCEEEHHHHHHHHHHCCCCCHHHHEEEEEEECCCCHHCCCCCCHHHHHHHHHHHCCCCCC
GAAKVGGNYAGSLLPGKAAKSAGFSDVIYLDPATHTKIEEVGAANFFGITANNEFVTPLS
CHHHCCCCCCCCCCCCCHHCCCCCCEEEEECCCCCCHHHHCCCCEEEEEECCCCEECCCC
PSILPSITKYSLLQLAEERLGMTVIEGDVPINELDKFVEAGACGTAAVISPIGGIQYKDN
CHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHCCCCCCHHHHHCCCCCEECCC
LHVFYSETEVGPVTRRLYDELVGIQFGDIEAPEGWIVKVD
EEEEEECCCCCHHHHHHHHHHHCCEECCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]