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The map label for this gene is cpsY [H]

Identifier: 15674920

GI number: 15674920

Start: 742702

End: 743607

Strand: Reverse

Name: cpsY [H]

Synonym: SPy_0898

Alternate gene names: 15674920

Gene position: 743607-742702 (Counterclockwise)

Preceding gene: 15674929

Following gene: 15674913

Centisome position: 40.14

GC content: 39.74

Gene sequence:

>906_bases
ATGAGAATTCAACAACTGCATTATATTATCAAAATTGTTGAGTGCGGCTCTATGAATGAAGCTGCTAAGCAATTATTTAT
CACACAACCAAGTCTATCCAATGCGGTCAAGGATTTGGAAATGGAGATGGGCATTACCATTTTCAACCGAAATCCTAAAG
GGATCACCTTGACAAAAGATGGGGTGGAATTTTTATCTTATGCTCGCCAAATTATAGAGCAAACATCACTGTTAGAAGAC
CGCTATAAAAACCACAATACTGGCCGAGAGCTCTTTAGCGTTTCATCTCAACATTATGCCTTCGTAGTCAATGCATTTGT
CTCACTCCTCAAGCGGACCGACATGACTCGGTATGAACTCTTTTTGCGCGAGACAAGAACTTGGGAAATCATTGATGATG
TCAAAAACTTTCGATCTGAAATTGGTGTTCTCTTTATCAACGACTATAACCGAGATGTCTTGACCAAATTATTTGATGAC
AATCATTTGACCGCCTCTCCATTATTTAAGGCACAGCCTCATATTTTTGTCAGCAAATCAAATCCTCTAGCAACTAAAAG
CCTATTAAGCATGGACGACTTGAGAGATTTTCCTTACCTGAGCTATGACCAAGGGATTCACAACTCCTTTTATTTTTCAG
AAGAGATGATGTCCCAGATGCCCCACAACAAATCAATTGTGGTCAGTGACCGTGCCACCCTCTTTAACTTAATGATTGGT
TTAGATGGCTACACCGTTGCCAGTGGTATTCTAAATAGCAATCTAAATGGTGACCAGATTGTGGCTATCCCGCTAGATGT
CCCTGACGAAATTGACATTGTTTTTATCAAGCATGAAAAAGCCAACTTGTCCAAAATGGGGGAACGTTTTATCGAATACC
TTCTCGAAGAAGTAACCTTTGATTAA

Upstream 100 bases:

>100_bases
TAGGCCATATTGAGAAAAGACTCCAGCGCTTTTTCAAAGTTCTAAACCCATGATGACGTTTGTGCTTGCGTCTTTATTTT
TTTTGAGCTAGACTGTACTT

Downstream 100 bases:

>100_bases
TGTCTTTTTCAATCGTCATACTAAAAAACACGGTAGTAAGACCTGTATCGGTCAAGTTATCGTGTTTTTTGATAAATATC
AGACAATAGATTGTCTCCAA

Product: putative transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MRIQQLHYIIKIVECGSMNEAAKQLFITQPSLSNAVKDLEMEMGITIFNRNPKGITLTKDGVEFLSYARQIIEQTSLLED
RYKNHNTGRELFSVSSQHYAFVVNAFVSLLKRTDMTRYELFLRETRTWEIIDDVKNFRSEIGVLFINDYNRDVLTKLFDD
NHLTASPLFKAQPHIFVSKSNPLATKSLLSMDDLRDFPYLSYDQGIHNSFYFSEEMMSQMPHNKSIVVSDRATLFNLMIG
LDGYTVASGILNSNLNGDQIVAIPLDVPDEIDIVFIKHEKANLSKMGERFIEYLLEEVTFD

Sequences:

>Translated_301_residues
MRIQQLHYIIKIVECGSMNEAAKQLFITQPSLSNAVKDLEMEMGITIFNRNPKGITLTKDGVEFLSYARQIIEQTSLLED
RYKNHNTGRELFSVSSQHYAFVVNAFVSLLKRTDMTRYELFLRETRTWEIIDDVKNFRSEIGVLFINDYNRDVLTKLFDD
NHLTASPLFKAQPHIFVSKSNPLATKSLLSMDDLRDFPYLSYDQGIHNSFYFSEEMMSQMPHNKSIVVSDRATLFNLMIG
LDGYTVASGILNSNLNGDQIVAIPLDVPDEIDIVFIKHEKANLSKMGERFIEYLLEEVTFD
>Mature_301_residues
MRIQQLHYIIKIVECGSMNEAAKQLFITQPSLSNAVKDLEMEMGITIFNRNPKGITLTKDGVEFLSYARQIIEQTSLLED
RYKNHNTGRELFSVSSQHYAFVVNAFVSLLKRTDMTRYELFLRETRTWEIIDDVKNFRSEIGVLFINDYNRDVLTKLFDD
NHLTASPLFKAQPHIFVSKSNPLATKSLLSMDDLRDFPYLSYDQGIHNSFYFSEEMMSQMPHNKSIVVSDRATLFNLMIG
LDGYTVASGILNSNLNGDQIVAIPLDVPDEIDIVFIKHEKANLSKMGERFIEYLLEEVTFD

Specific function: Seems to regulate utilization of fixed nitrogen by controlling the expression of a certain gene(s) involved in nitrogen metabolism [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787530, Length=214, Percent_Identity=25.2336448598131, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1788296, Length=217, Percent_Identity=27.6497695852535, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788481, Length=294, Percent_Identity=18.7074829931973, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34678; Mature: 34678

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIQQLHYIIKIVECGSMNEAAKQLFITQPSLSNAVKDLEMEMGITIFNRNPKGITLTKD
CCHHHHHHHHHHHHCCCCCHHHHHHEEECCCHHHHHHHHHHHCCEEEEECCCCCEEEEHH
GVEFLSYARQIIEQTSLLEDRYKNHNTGRELFSVSSQHYAFVVNAFVSLLKRTDMTRYEL
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHH
FLRETRTWEIIDDVKNFRSEIGVLFINDYNRDVLTKLFDDNHLTASPLFKAQPHIFVSKS
HHHHCCCHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCEEEECC
NPLATKSLLSMDDLRDFPYLSYDQGIHNSFYFSEEMMSQMPHNKSIVVSDRATLFNLMIG
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEECHHHHHHCCCCCEEEEECHHHHHHHHHC
LDGYTVASGILNSNLNGDQIVAIPLDVPDEIDIVFIKHEKANLSKMGERFIEYLLEEVTF
CCCHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCC
D
C
>Mature Secondary Structure
MRIQQLHYIIKIVECGSMNEAAKQLFITQPSLSNAVKDLEMEMGITIFNRNPKGITLTKD
CCHHHHHHHHHHHHCCCCCHHHHHHEEECCCHHHHHHHHHHHCCEEEEECCCCCEEEEHH
GVEFLSYARQIIEQTSLLEDRYKNHNTGRELFSVSSQHYAFVVNAFVSLLKRTDMTRYEL
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHH
FLRETRTWEIIDDVKNFRSEIGVLFINDYNRDVLTKLFDDNHLTASPLFKAQPHIFVSKS
HHHHCCCHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCEEEECC
NPLATKSLLSMDDLRDFPYLSYDQGIHNSFYFSEEMMSQMPHNKSIVVSDRATLFNLMIG
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEECHHHHHHCCCCCEEEEECHHHHHHHHHC
LDGYTVASGILNSNLNGDQIVAIPLDVPDEIDIVFIKHEKANLSKMGERFIEYLLEEVTF
CCCHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCC
D
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]