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The map label for this gene is punA [H]

Identifier: 15674917

GI number: 15674917

Start: 739657

End: 740466

Strand: Direct

Name: punA [H]

Synonym: SPy_0892

Alternate gene names: 15674917

Gene position: 739657-740466 (Clockwise)

Preceding gene: 15674916

Following gene: 15674918

Centisome position: 39.93

GC content: 41.36

Gene sequence:

>810_bases
ATGTCATTGATGACTAAAATTAACGAAACAAAAGACTTTTTAGTTACTAAAGGAATTGAAACTCCAGAATTTGGATTAAT
TCTGGGTTCAGGCCTTGGTGAACTTGCTGAAGAAGTAGAAAATGCTATTGTTATTGATTATGCAGATATTCCAAACTGGG
GCAAGTCAACGGTTGTTGGCCATGCTGGAAAGTTAGTTTACGGAGATTTAGCTGGCCGTAAAGTGCTCGCCTTACAAGGA
CGTTTCCATTTTTACGAAGGAAATCCGCTTGAAGTGGTGACTTTCCCTGTTCGTGTCATGAAAGCTCTGGGATGTGAAGG
CGTTCTTGTCACAAATGCAGCTGGTGGTATCGGCTACGGCCCAGGTACCTTGATGGCAATCACAGACCATATCAATATGA
CCGGAAATAACCCACTAATCGGTGAAAACTTGGACGAGTTTGGTCCACGCTTTCCTGATATGTCTGATGCCTATACTAAG
GTTTATCGCAATAAGGCACATGAAGTGGCTGAAAAAATGAACATCAAACTAGAAGATGGTGTTTATATGGGCTTGACTGG
TCCAACTTATGAAACACCAGCAGAAATCCGTGCTTTCAAAGTATTAGGAGCAGATGCTGTTGGCATGTCAACTGTACCGG
AAGTTATTGTGGCTGCTCATTCTGGACTTAAAGTGCTTGGTATTTCAGCTATTACAAACTTTGCAGCTGGTTTCCAATCA
GAATTGAACCATGAAGAAGTTGTGGAAGTGACCCAACATATCAAAGAAGATTTCAAAGGTCTTGTTAAAGCTATTTTAGC
GGAGTTGTAG

Upstream 100 bases:

>100_bases
TCTGAGACCTTACTGGATATTCTTCCTGTTCCCCTGCCTAGTCCTTATATTAAAGAGGATGGCGAGAGTGTGAACCCTAT
TGAGGTAAAAGGAGAAAAAG

Downstream 100 bases:

>100_bases
GACGAACAAAATCAACTGGAGCATTATTACAGGAATGTTATTTTATGTCGTCAGTTTGGCACATCTGTTCGCAATAAAAA
AATCAAAAAATAATCACGGA

Product: purine nucleoside phosphorylase

Products: NA

Alternate protein names: Inosine phosphorylase; Purine nucleoside phosphorylase I; PNP I; PU-NPase I [H]

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MSLMTKINETKDFLVTKGIETPEFGLILGSGLGELAEEVENAIVIDYADIPNWGKSTVVGHAGKLVYGDLAGRKVLALQG
RFHFYEGNPLEVVTFPVRVMKALGCEGVLVTNAAGGIGYGPGTLMAITDHINMTGNNPLIGENLDEFGPRFPDMSDAYTK
VYRNKAHEVAEKMNIKLEDGVYMGLTGPTYETPAEIRAFKVLGADAVGMSTVPEVIVAAHSGLKVLGISAITNFAAGFQS
ELNHEEVVEVTQHIKEDFKGLVKAILAEL

Sequences:

>Translated_269_residues
MSLMTKINETKDFLVTKGIETPEFGLILGSGLGELAEEVENAIVIDYADIPNWGKSTVVGHAGKLVYGDLAGRKVLALQG
RFHFYEGNPLEVVTFPVRVMKALGCEGVLVTNAAGGIGYGPGTLMAITDHINMTGNNPLIGENLDEFGPRFPDMSDAYTK
VYRNKAHEVAEKMNIKLEDGVYMGLTGPTYETPAEIRAFKVLGADAVGMSTVPEVIVAAHSGLKVLGISAITNFAAGFQS
ELNHEEVVEVTQHIKEDFKGLVKAILAEL
>Mature_268_residues
SLMTKINETKDFLVTKGIETPEFGLILGSGLGELAEEVENAIVIDYADIPNWGKSTVVGHAGKLVYGDLAGRKVLALQGR
FHFYEGNPLEVVTFPVRVMKALGCEGVLVTNAAGGIGYGPGTLMAITDHINMTGNNPLIGENLDEFGPRFPDMSDAYTKV
YRNKAHEVAEKMNIKLEDGVYMGLTGPTYETPAEIRAFKVLGADAVGMSTVPEVIVAAHSGLKVLGISAITNFAAGFQSE
LNHEEVVEVTQHIKEDFKGLVKAILAEL

Specific function: Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules [H]

COG id: COG0005

COG function: function code F; Purine nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/MTAP phosphorylase family [H]

Homologues:

Organism=Homo sapiens, GI157168362, Length=270, Percent_Identity=46.2962962962963, Blast_Score=235, Evalue=3e-62,
Organism=Homo sapiens, GI47132622, Length=246, Percent_Identity=27.2357723577236, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1788746, Length=252, Percent_Identity=44.047619047619, Blast_Score=215, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI17541190, Length=264, Percent_Identity=44.3181818181818, Blast_Score=209, Evalue=1e-54,
Organism=Saccharomyces cerevisiae, GI6323238, Length=283, Percent_Identity=41.3427561837456, Blast_Score=192, Evalue=6e-50,
Organism=Drosophila melanogaster, GI45552885, Length=275, Percent_Identity=46.5454545454545, Blast_Score=229, Evalue=2e-60,
Organism=Drosophila melanogaster, GI45552887, Length=275, Percent_Identity=46.5454545454545, Blast_Score=228, Evalue=3e-60,
Organism=Drosophila melanogaster, GI24656093, Length=275, Percent_Identity=46.5454545454545, Blast_Score=228, Evalue=3e-60,
Organism=Drosophila melanogaster, GI24656090, Length=275, Percent_Identity=46.5454545454545, Blast_Score=228, Evalue=3e-60,
Organism=Drosophila melanogaster, GI24762376, Length=227, Percent_Identity=37.8854625550661, Blast_Score=137, Evalue=5e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011268
- InterPro:   IPR000845
- InterPro:   IPR011270
- InterPro:   IPR001369
- InterPro:   IPR018099 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: =2.4.2.1 [H]

Molecular weight: Translated: 28882; Mature: 28750

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS01240 PNP_MTAP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLMTKINETKDFLVTKGIETPEFGLILGSGLGELAEEVENAIVIDYADIPNWGKSTVVG
CCCHHHHCCCHHHEEECCCCCCCCCEEECCCHHHHHHHHCCEEEEEECCCCCCCCCCEEC
HAGKLVYGDLAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKALGCEGVLVTNAAGGIGYG
CCCCEEEECCCCCEEEEEECEEEEECCCCEEEEEHHHHHHHHCCCCCEEEECCCCCCCCC
PGTLMAITDHINMTGNNPLIGENLDEFGPRFPDMSDAYTKVYRNKAHEVAEKMNIKLEDG
CCEEEEEEEEEEECCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCEECCC
VYMGLTGPTYETPAEIRAFKVLGADAVGMSTVPEVIVAAHSGLKVLGISAITNFAAGFQS
EEEECCCCCCCCCHHHEEEEEECCCCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHH
ELNHEEVVEVTQHIKEDFKGLVKAILAEL
HCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SLMTKINETKDFLVTKGIETPEFGLILGSGLGELAEEVENAIVIDYADIPNWGKSTVVG
CCHHHHCCCHHHEEECCCCCCCCCEEECCCHHHHHHHHCCEEEEEECCCCCCCCCCEEC
HAGKLVYGDLAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKALGCEGVLVTNAAGGIGYG
CCCCEEEECCCCCEEEEEECEEEEECCCCEEEEEHHHHHHHHCCCCCEEEECCCCCCCCC
PGTLMAITDHINMTGNNPLIGENLDEFGPRFPDMSDAYTKVYRNKAHEVAEKMNIKLEDG
CCEEEEEEEEEEECCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCEECCC
VYMGLTGPTYETPAEIRAFKVLGADAVGMSTVPEVIVAAHSGLKVLGISAITNFAAGFQS
EEEECCCCCCCCCHHHEEEEEECCCCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHH
ELNHEEVVEVTQHIKEDFKGLVKAILAEL
HCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9058965 [H]