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The map label for this gene is apbA

Identifier: 15674885

GI number: 15674885

Start: 703497

End: 704420

Strand: Direct

Name: apbA

Synonym: SPy_0852

Alternate gene names: 15674885

Gene position: 703497-704420 (Clockwise)

Preceding gene: 15674884

Following gene: 15674886

Centisome position: 37.98

GC content: 40.26

Gene sequence:

>924_bases
ATGTTAGTTTATATTGCTGGCTCAGGAGCCATGGGGTGCCGTTTTGGTTACCAAATTTCAAAAACAAATAACGATGTGAT
TTTGTTAGATAACTGGGAAGATCATATCAATGCCATTAAAGAAAACGGTTTAGTGGTGACAGGGGATGTGGAAGAAACAG
TTAAGCTTCCTATTATGAAACCGACTGAAGCCACTCAAGAAGCCGATTTGATTATCTTGTTTACAAAAGCAATGCAGTTG
CCACAAATGCTTCAAGACATTAAAGGCATTATTGGAAAAGAAACCAAAGTGCTTTGCCTTTTAAATGGTCTTGGACACGA
AGACGTTATTCGTCAGTACATTCCAGAGCACAACATTTTGATGGGGGTCACTGTCTGGACTGCTGGTCTAGAAGGTCCTG
GTCGTGCTCATCTTCAAGGTGTGGGAGCACTTAACTTACAAAGCATGGATCCAAGCAATCAAGAAGCTGGACACCAAGTG
GCTGATTTGTTAAATGAAGCTAACTTGAATGCGACTTATGATGAGAATGTTGTGCCAAACATCTGGCGAAAAGCTTGCGT
GAATGGTACCATGAACTCAACTTGTGCCCTTCTAGATTGTACAATTGGTGAATTATTTGCCAGTGAAGACGGTCTCAAAA
TGGTAAAAGAAATCATCCATGAGTTTGTTATCGTTGGTCAAGCAGAAGGTGTCGAACTAAACGAAGAAGAAATCACACAA
TATGTAATGGATACATCTGTTAAAGCTGCTCACCATTACCCATCAATGCACCAAGACCTTGTGCAAAATCACCGATTGAC
TGAAATTGATTTTATCAATGGTGCAGTCAATACCAAAGGTGAAAAGCTTGGTATCAATACTCCATACTGCCGTATGATTA
CAGAGCTAGTTCACGCTAAAGAAGCAGTGTTGAACATCCAATAA

Upstream 100 bases:

>100_bases
TCCGTTTGCGGTTGCTTTTGCGGCGCATAAGGTTTCTAAAGATATTCTAAAACTGTATAAAGAAGACATTTTCGTCTTTG
AAGGCTAATAGGAGGAAAAT

Downstream 100 bases:

>100_bases
ACGCTATCAAATCGTTATACTCAATATTATGGCTAAAGCTTTAATATTGAGTTTTTTGTTATGCTGATAGTATTTTTCTT
AGGCGTCATTATTTGTGAGC

Product: 2-dehydropantoate 2-reductase

Products: NA

Alternate protein names: Ketopantoate reductase; KPA reductase; KPR

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MLVYIAGSGAMGCRFGYQISKTNNDVILLDNWEDHINAIKENGLVVTGDVEETVKLPIMKPTEATQEADLIILFTKAMQL
PQMLQDIKGIIGKETKVLCLLNGLGHEDVIRQYIPEHNILMGVTVWTAGLEGPGRAHLQGVGALNLQSMDPSNQEAGHQV
ADLLNEANLNATYDENVVPNIWRKACVNGTMNSTCALLDCTIGELFASEDGLKMVKEIIHEFVIVGQAEGVELNEEEITQ
YVMDTSVKAAHHYPSMHQDLVQNHRLTEIDFINGAVNTKGEKLGINTPYCRMITELVHAKEAVLNIQ

Sequences:

>Translated_307_residues
MLVYIAGSGAMGCRFGYQISKTNNDVILLDNWEDHINAIKENGLVVTGDVEETVKLPIMKPTEATQEADLIILFTKAMQL
PQMLQDIKGIIGKETKVLCLLNGLGHEDVIRQYIPEHNILMGVTVWTAGLEGPGRAHLQGVGALNLQSMDPSNQEAGHQV
ADLLNEANLNATYDENVVPNIWRKACVNGTMNSTCALLDCTIGELFASEDGLKMVKEIIHEFVIVGQAEGVELNEEEITQ
YVMDTSVKAAHHYPSMHQDLVQNHRLTEIDFINGAVNTKGEKLGINTPYCRMITELVHAKEAVLNIQ
>Mature_307_residues
MLVYIAGSGAMGCRFGYQISKTNNDVILLDNWEDHINAIKENGLVVTGDVEETVKLPIMKPTEATQEADLIILFTKAMQL
PQMLQDIKGIIGKETKVLCLLNGLGHEDVIRQYIPEHNILMGVTVWTAGLEGPGRAHLQGVGALNLQSMDPSNQEAGHQV
ADLLNEANLNATYDENVVPNIWRKACVNGTMNSTCALLDCTIGELFASEDGLKMVKEIIHEFVIVGQAEGVELNEEEITQ
YVMDTSVKAAHHYPSMHQDLVQNHRLTEIDFINGAVNTKGEKLGINTPYCRMITELVHAKEAVLNIQ

Specific function: Catalyzes the NADPH-dependent reduction of ketopantoate into pantoic acid

COG id: COG1893

COG function: function code H; Ketopantoate reductase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ketopantoate reductase family

Homologues:

Organism=Escherichia coli, GI1786627, Length=237, Percent_Identity=27.4261603375527, Blast_Score=82, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PANE_STRP1 (P65666)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_269059.1
- RefSeq:   YP_282022.1
- ProteinModelPortal:   P65666
- SMR:   P65666
- EnsemblBacteria:   EBSTRT00000000038
- EnsemblBacteria:   EBSTRT00000028707
- GeneID:   3572249
- GeneID:   901016
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_0852
- KEGG:   spz:M5005_Spy_0659
- GeneTree:   EBGT00050000027425
- HOGENOM:   HBG668370
- OMA:   VWATIRE
- ProtClustDB:   PRK06522
- BioCyc:   SPYO160490:SPY0852-MONOMER
- BioCyc:   SPYO293653:M5005_SPY0659-MONOMER
- GO:   GO:0005737
- InterPro:   IPR008927
- InterPro:   IPR003710
- InterPro:   IPR013752
- InterPro:   IPR013332
- InterPro:   IPR013328
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- Gene3D:   G3DSA:1.10.1040.10
- PANTHER:   PTHR21708:SF21
- TIGRFAMs:   TIGR00745

Pfam domain/function: PF02558 ApbA; PF08546 ApbA_C; SSF48179 6DGDH_C_like

EC number: =1.1.1.169

Molecular weight: Translated: 33829; Mature: 33829

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: ACT_SITE 184-184 BINDING 102-102 BINDING 102-102 BINDING 188-188 BINDING 192-192 BINDING 255-255 BINDING 268-268

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVYIAGSGAMGCRFGYQISKTNNDVILLDNWEDHINAIKENGLVVTGDVEETVKLPIMK
CEEEEECCCCCCEEECEEEECCCCCEEEEECCHHHHHHHHHCCEEEECCHHHHEECCCCC
PTEATQEADLIILFTKAMQLPQMLQDIKGIIGKETKVLCLLNGLGHEDVIRQYIPEHNIL
CCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCCCCEE
MGVTVWTAGLEGPGRAHLQGVGALNLQSMDPSNQEAGHQVADLLNEANLNATYDENVVPN
EEEEEEECCCCCCCCHHEEECCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHH
IWRKACVNGTMNSTCALLDCTIGELFASEDGLKMVKEIIHEFVIVGQAEGVELNEEEITQ
HHHHHHCCCCCCCCEEEEECHHHHHHCCCCCHHHHHHHHHHHEEECCCCCCCCCHHHHHH
YVMDTSVKAAHHYPSMHQDLVQNHRLTEIDFINGAVNTKGEKLGINTPYCRMITELVHAK
HHHHCCHHHHHCCCHHHHHHHHCCCCCEEEEECCCCCCCCCEECCCCHHHHHHHHHHHHH
EAVLNIQ
HHHEECC
>Mature Secondary Structure
MLVYIAGSGAMGCRFGYQISKTNNDVILLDNWEDHINAIKENGLVVTGDVEETVKLPIMK
CEEEEECCCCCCEEECEEEECCCCCEEEEECCHHHHHHHHHCCEEEECCHHHHEECCCCC
PTEATQEADLIILFTKAMQLPQMLQDIKGIIGKETKVLCLLNGLGHEDVIRQYIPEHNIL
CCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCCCCEE
MGVTVWTAGLEGPGRAHLQGVGALNLQSMDPSNQEAGHQVADLLNEANLNATYDENVVPN
EEEEEEECCCCCCCCHHEEECCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHH
IWRKACVNGTMNSTCALLDCTIGELFASEDGLKMVKEIIHEFVIVGQAEGVELNEEEITQ
HHHHHHCCCCCCCCEEEEECHHHHHHCCCCCHHHHHHHHHHHEEECCCCCCCCCHHHHHH
YVMDTSVKAAHHYPSMHQDLVQNHRLTEIDFINGAVNTKGEKLGINTPYCRMITELVHAK
HHHHCCHHHHHCCCHHHHHHHHCCCCCEEEEECCCCCCCCCEECCCCHHHHHHHHHHHHH
EAVLNIQ
HHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11296296