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The map label for this gene is nrdE.1 [H]

Identifier: 15674554

GI number: 15674554

Start: 354748

End: 356928

Strand: Direct

Name: nrdE.1 [H]

Synonym: SPy_0427

Alternate gene names: 15674554

Gene position: 354748-356928 (Clockwise)

Preceding gene: 15674553

Following gene: 15674556

Centisome position: 19.15

GC content: 45.48

Gene sequence:

>2181_bases
ATGTCACAAACCAATGCGTCATACCTCTCATTAAATGCACTGACCCGTTTTAAAAAACCAGACGGCAGCTACCATTTTGA
TAGCGATAAAGAAGCTGTTAGGCGCTACCTGGAAGAACATGTCTCTCCCAATCAAATGGCTTTTAACTCACTAGAAGATA
AGCTAGCTTACCTCATCAATGAAGGGTATTATGAGCAGGCCATTTTTGATGCCTATCCTAATGACCTTATCAAGGAGGCC
TTTCATTATGCCTATCAACAAGGCTATCGCTTTTTAAATCTCATGGGCGCTATGAAATTTTACCAGTCCTATGCCTTAAA
AACCTTGGACGGTAAGCAGTATCTAGAAACATTTGAAGACCGTGCTGTGATGAATGCACTCTTTTTAGCTGATGGAGATC
AAACCTTTGTCTTTGACGTGATTGATGCCATCTTGCACCGACGCTTTCAACCAGCTACCCCAACGTTTTTAAATGCCGGA
AAAAAACGCCGTGGCGAATACATTTCGTGCTATCTTCTTCGTGTCGAAGACAATATGGAATCTATCTCTCGCGCCATTAG
CACCAGCTTGCAACTCTCAAAAAGAGGAGGCGGAGTCGCCTTGTGCCTCACCAACCTCAGAGAAATCGGAGCACCGATAA
AAGGGATTGAAAATCAGGCAACAGGCATCGTTCCTGTCATGAAACTACTAGAGGACTCTTTTTCCTATGCCAATCAACTA
GGTCAGCGCCAAGGAGCAGGTGCGGTTTACCTTCATGCCCATCACCCTGAGGTTTTGACCTTTTTAGACACCAAACGTGA
AAATGCCGATGAGAAAATTCGCATTAAGTCCCTAGCATTGGGACTTGTGATCCCAGATATTACCTTCCAGTTAGCCAAAG
AAAACAAAGACATGGCACTCTTTTCACCCTATGATATCAAGCGTGCCTATGGCAAGGACATGTCAGACATCTCCATCACA
GAGGAATACGATAAACTGCTAGCCAATCCAGCCATCAAAAAGACCTACATCAGTGCTAGAAAGTTCTTTCAGTTAATTGC
AGAGCTACATTTTGAGTCAGGCTACCCTTACCTCTTGTTTGATGACACGGTCAATAAGCGCAACCCTCATGCCAAAAAAG
GCAGAATCGTCATGTCTAACCTGTGTTCTGAAATCGCTCAAGTTAGCACCCCAAGCACTTTCAAAGAAGATTTGAGTTTT
GAAACCATTGGAGAGGATATCTGCTGTAATTTAGGGTCTATCAATATTGCCCAGGCTATGGCAGATGCGCCACACTTTGA
ACAGCTCATCACAACGAGCATCAGAGCACTTGATCGTGTCTCTAGAGTGTCTGACCTGAATTGTGCGCCTTCTGTTGAGA
CAGGCAATGCAGCCAACCATGCAGTAGGATTAGGCGCCATGAACCTACATGGTTTTTTGGCAACCAACCACATCTACTAT
GATACAAAAGAAGCCGTTGACTTTACAGATCTGTTTTTCCATGCCATGGCCTATTATGCTTTTAAAGCCTCTTGCCAATT
AGCCAAAGAAAAAGGCGCTTTTGCAGGATTTTCGTTATCAACCTATTCAGATGGCACTTATTTTGCCAAGTATTTGCAAG
AAGATGCCAAGCCACAAACAGCCAAGGTTGCCACATTGTTGCAAGATTACGGCTTCACGTTGCCAACGGTGGCGGATTGG
CAAGCCCTAGTAGCAGATATCAAGCAGTTTGGCCTAGCCAATGCGCACTTGCTAGCGGTTGCTCCGACAGGCTCCATCAG
CTACTTATCATCCTGCACTCCGAGTCTACAACCAGTCGTTGCTCCTGTTGAGGTCCGAAAAGAAGGTAGCCTGGGCAGGA
TTTACGTCCCTGCCTATCAGATTGACCAGGCTAACTATGCTTACTATGAACGTGGTGCCTATGAGGTCGGCCCAAAAGCC
ATCATCGACGTTGTAGCTGCTGCCCAAAAACACGTTGACCAAGCCATCTCATTGACCCTGTTTATGACCGATCAAGCGAC
CACACGTGACCTGAACCGCTCCTATATCCAAGCTTTTAAGCAAAATTGTGCATCGATCTATTATGTGCGAGTGAGGCAAG
ACGTCCTAGCAGGCAGCGAACAGTATGACGAGGATAGTCTTGTGACAGCTCCTGGAGCTAGTGATGAGACCACCACAGAG
TGTCAAAGCTGTATGATCTAA

Upstream 100 bases:

>100_bases
CAGATGTCAAAAAGGTACAGGCGATTTTTGCCCGCTTAAAGCATCACACACACGACAAGCAAAAACAAACCAACAACCTG
ATTACAGAAAGGACACACCC

Downstream 100 bases:

>100_bases
CAGCTACTAGAAAGCTCTCGTGTCCCCTATCACATGCATAGGATCAGTGCACTCGACCTTTCAAGACAAGCAAGCATCAG
CTCTTGCTTGTCTTTTTTTG

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: Ribonucleotide reductase [H]

Number of amino acids: Translated: 726; Mature: 725

Protein sequence:

>726_residues
MSQTNASYLSLNALTRFKKPDGSYHFDSDKEAVRRYLEEHVSPNQMAFNSLEDKLAYLINEGYYEQAIFDAYPNDLIKEA
FHYAYQQGYRFLNLMGAMKFYQSYALKTLDGKQYLETFEDRAVMNALFLADGDQTFVFDVIDAILHRRFQPATPTFLNAG
KKRRGEYISCYLLRVEDNMESISRAISTSLQLSKRGGGVALCLTNLREIGAPIKGIENQATGIVPVMKLLEDSFSYANQL
GQRQGAGAVYLHAHHPEVLTFLDTKRENADEKIRIKSLALGLVIPDITFQLAKENKDMALFSPYDIKRAYGKDMSDISIT
EEYDKLLANPAIKKTYISARKFFQLIAELHFESGYPYLLFDDTVNKRNPHAKKGRIVMSNLCSEIAQVSTPSTFKEDLSF
ETIGEDICCNLGSINIAQAMADAPHFEQLITTSIRALDRVSRVSDLNCAPSVETGNAANHAVGLGAMNLHGFLATNHIYY
DTKEAVDFTDLFFHAMAYYAFKASCQLAKEKGAFAGFSLSTYSDGTYFAKYLQEDAKPQTAKVATLLQDYGFTLPTVADW
QALVADIKQFGLANAHLLAVAPTGSISYLSSCTPSLQPVVAPVEVRKEGSLGRIYVPAYQIDQANYAYYERGAYEVGPKA
IIDVVAAAQKHVDQAISLTLFMTDQATTRDLNRSYIQAFKQNCASIYYVRVRQDVLAGSEQYDEDSLVTAPGASDETTTE
CQSCMI

Sequences:

>Translated_726_residues
MSQTNASYLSLNALTRFKKPDGSYHFDSDKEAVRRYLEEHVSPNQMAFNSLEDKLAYLINEGYYEQAIFDAYPNDLIKEA
FHYAYQQGYRFLNLMGAMKFYQSYALKTLDGKQYLETFEDRAVMNALFLADGDQTFVFDVIDAILHRRFQPATPTFLNAG
KKRRGEYISCYLLRVEDNMESISRAISTSLQLSKRGGGVALCLTNLREIGAPIKGIENQATGIVPVMKLLEDSFSYANQL
GQRQGAGAVYLHAHHPEVLTFLDTKRENADEKIRIKSLALGLVIPDITFQLAKENKDMALFSPYDIKRAYGKDMSDISIT
EEYDKLLANPAIKKTYISARKFFQLIAELHFESGYPYLLFDDTVNKRNPHAKKGRIVMSNLCSEIAQVSTPSTFKEDLSF
ETIGEDICCNLGSINIAQAMADAPHFEQLITTSIRALDRVSRVSDLNCAPSVETGNAANHAVGLGAMNLHGFLATNHIYY
DTKEAVDFTDLFFHAMAYYAFKASCQLAKEKGAFAGFSLSTYSDGTYFAKYLQEDAKPQTAKVATLLQDYGFTLPTVADW
QALVADIKQFGLANAHLLAVAPTGSISYLSSCTPSLQPVVAPVEVRKEGSLGRIYVPAYQIDQANYAYYERGAYEVGPKA
IIDVVAAAQKHVDQAISLTLFMTDQATTRDLNRSYIQAFKQNCASIYYVRVRQDVLAGSEQYDEDSLVTAPGASDETTTE
CQSCMI
>Mature_725_residues
SQTNASYLSLNALTRFKKPDGSYHFDSDKEAVRRYLEEHVSPNQMAFNSLEDKLAYLINEGYYEQAIFDAYPNDLIKEAF
HYAYQQGYRFLNLMGAMKFYQSYALKTLDGKQYLETFEDRAVMNALFLADGDQTFVFDVIDAILHRRFQPATPTFLNAGK
KRRGEYISCYLLRVEDNMESISRAISTSLQLSKRGGGVALCLTNLREIGAPIKGIENQATGIVPVMKLLEDSFSYANQLG
QRQGAGAVYLHAHHPEVLTFLDTKRENADEKIRIKSLALGLVIPDITFQLAKENKDMALFSPYDIKRAYGKDMSDISITE
EYDKLLANPAIKKTYISARKFFQLIAELHFESGYPYLLFDDTVNKRNPHAKKGRIVMSNLCSEIAQVSTPSTFKEDLSFE
TIGEDICCNLGSINIAQAMADAPHFEQLITTSIRALDRVSRVSDLNCAPSVETGNAANHAVGLGAMNLHGFLATNHIYYD
TKEAVDFTDLFFHAMAYYAFKASCQLAKEKGAFAGFSLSTYSDGTYFAKYLQEDAKPQTAKVATLLQDYGFTLPTVADWQ
ALVADIKQFGLANAHLLAVAPTGSISYLSSCTPSLQPVVAPVEVRKEGSLGRIYVPAYQIDQANYAYYERGAYEVGPKAI
IDVVAAAQKHVDQAISLTLFMTDQATTRDLNRSYIQAFKQNCASIYYVRVRQDVLAGSEQYDEDSLVTAPGASDETTTEC
QSCMI

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase large chain family [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=589, Percent_Identity=24.4482173174873, Blast_Score=182, Evalue=1e-45,
Organism=Escherichia coli, GI1789030, Length=701, Percent_Identity=54.6362339514979, Blast_Score=817, Evalue=0.0,
Organism=Escherichia coli, GI2367133, Length=597, Percent_Identity=26.6331658291457, Blast_Score=181, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17555242, Length=669, Percent_Identity=23.9162929745889, Blast_Score=181, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6320914, Length=589, Percent_Identity=25.6366723259762, Blast_Score=180, Evalue=7e-46,
Organism=Saccharomyces cerevisiae, GI6322123, Length=611, Percent_Identity=24.2225859247136, Blast_Score=178, Evalue=3e-45,
Organism=Drosophila melanogaster, GI17352481, Length=472, Percent_Identity=27.9661016949153, Blast_Score=172, Evalue=6e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926
- InterPro:   IPR013554 [H]

Pfam domain/function: PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN; PF08343 RNR_N [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 80969; Mature: 80838

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTNASYLSLNALTRFKKPDGSYHFDSDKEAVRRYLEEHVSPNQMAFNSLEDKLAYLIN
CCCCCCHHEEHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHC
EGYYEQAIFDAYPNDLIKEAFHYAYQQGYRFLNLMGAMKFYQSYALKTLDGKQYLETFED
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
RAVMNALFLADGDQTFVFDVIDAILHRRFQPATPTFLNAGKKRRGEYISCYLLRVEDNME
HHHHHHHEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHCCHHHCCCEEEEEEEEECCCHH
SISRAISTSLQLSKRGGGVALCLTNLREIGAPIKGIENQATGIVPVMKLLEDSFSYANQL
HHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
GQRQGAGAVYLHAHHPEVLTFLDTKRENADEKIRIKSLALGLVIPDITFQLAKENKDMAL
HHCCCCCEEEEEECCCCHHHHHHCCCCCCCCCEEHHHHHHHHEECCHHHHHHHCCCCEEE
FSPYDIKRAYGKDMSDISITEEYDKLLANPAIKKTYISARKFFQLIAELHFESGYPYLLF
ECCHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
DDTVNKRNPHAKKGRIVMSNLCSEIAQVSTPSTFKEDLSFETIGEDICCNLGSINIAQAM
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHCCCHHHCCHHHHCCCCCCHHHHHH
ADAPHFEQLITTSIRALDRVSRVSDLNCAPSVETGNAANHAVGLGAMNLHGFLATNHIYY
HCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCEECCCEEEECCEEE
DTKEAVDFTDLFFHAMAYYAFKASCQLAKEKGAFAGFSLSTYSDGTYFAKYLQEDAKPQT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHCCCCHH
AKVATLLQDYGFTLPTVADWQALVADIKQFGLANAHLLAVAPTGSISYLSSCTPSLQPVV
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHCCCCCCCCC
APVEVRKEGSLGRIYVPAYQIDQANYAYYERGAYEVGPKAIIDVVAAAQKHVDQAISLTL
CCHHCCCCCCCCEEEECEEEECCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEE
FMTDQATTRDLNRSYIQAFKQNCASIYYVRVRQDVLAGSEQYDEDSLVTAPGASDETTTE
EEECCHHHHHHHHHHHHHHHHHHHHEEEEEEHHHHHCCCCCCCCCCEEECCCCCCCCHHH
CQSCMI
HHHCCC
>Mature Secondary Structure 
SQTNASYLSLNALTRFKKPDGSYHFDSDKEAVRRYLEEHVSPNQMAFNSLEDKLAYLIN
CCCCCHHEEHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHC
EGYYEQAIFDAYPNDLIKEAFHYAYQQGYRFLNLMGAMKFYQSYALKTLDGKQYLETFED
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
RAVMNALFLADGDQTFVFDVIDAILHRRFQPATPTFLNAGKKRRGEYISCYLLRVEDNME
HHHHHHHEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHCCHHHCCCEEEEEEEEECCCHH
SISRAISTSLQLSKRGGGVALCLTNLREIGAPIKGIENQATGIVPVMKLLEDSFSYANQL
HHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
GQRQGAGAVYLHAHHPEVLTFLDTKRENADEKIRIKSLALGLVIPDITFQLAKENKDMAL
HHCCCCCEEEEEECCCCHHHHHHCCCCCCCCCEEHHHHHHHHEECCHHHHHHHCCCCEEE
FSPYDIKRAYGKDMSDISITEEYDKLLANPAIKKTYISARKFFQLIAELHFESGYPYLLF
ECCHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
DDTVNKRNPHAKKGRIVMSNLCSEIAQVSTPSTFKEDLSFETIGEDICCNLGSINIAQAM
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHCCCHHHCCHHHHCCCCCCHHHHHH
ADAPHFEQLITTSIRALDRVSRVSDLNCAPSVETGNAANHAVGLGAMNLHGFLATNHIYY
HCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCEECCCEEEECCEEE
DTKEAVDFTDLFFHAMAYYAFKASCQLAKEKGAFAGFSLSTYSDGTYFAKYLQEDAKPQT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHCCCCHH
AKVATLLQDYGFTLPTVADWQALVADIKQFGLANAHLLAVAPTGSISYLSSCTPSLQPVV
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHCCCCCCCCC
APVEVRKEGSLGRIYVPAYQIDQANYAYYERGAYEVGPKAIIDVVAAAQKHVDQAISLTL
CCHHCCCCCCCCEEEECEEEECCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEE
FMTDQATTRDLNRSYIQAFKQNCASIYYVRVRQDVLAGSEQYDEDSLVTAPGASDETTTE
EEECCHHHHHHHHHHHHHHHHHHHHEEEEEEHHHHHCCCCCCCCCCEEECCCCCCCCHHH
CQSCMI
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]