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The map label for this gene is lctO [C]

Identifier: 15674548

GI number: 15674548

Start: 342670

End: 343857

Strand: Direct

Name: lctO [C]

Synonym: SPy_0414

Alternate gene names: 15674548

Gene position: 342670-343857 (Clockwise)

Preceding gene: 15674546

Following gene: 15674549

Centisome position: 18.5

GC content: 42.09

Gene sequence:

>1188_bases
ATGTCAATGGCACAAAAAACAGTCATCACCGAAGAAACAACCGATTTTGTTATGGATTTCAAAACAAGTAGCGCTGAGGG
AAATGTTGATTTTATTAACGTTTTTGACCTTGAGAAAATGGCACAACAGGTCATCCCTAAAGGAGCCTTTGGTTATATTG
CTAGTGGTGCTGGTGATACCTTTACCCTCCATGAAAATATTCGCTCATTTAATCATAAATTAATTGTCCCTCACTCCCTC
AAAGGGGTTGAAAATCCGTCAACAGAGATTACGTTTGATGGTGATTACTTAACCTCTCCTCTTATTTTAGCACCTGTAGC
GGCTCATAAACTGGCCAATGAACAAGGAGAAGTAGCGTCTGCTAAAGGATTGAAGGAATTTGGAAGTATCTACACCACTA
GTTCCTACTCAACAACTGACCTGCCAGAAATCAGTGCTGCCTTAGGTGGGACACCCCATTGGTTTCAATTTTATTACAGT
AAAGACGATGGCATTAATCGTAATATTATGGATCGTGTCAAAGCACAAGGTTGCAAAGCTATCGTTTTGACAGCAGATGC
AACAGTTGGAGGAAACCGCGAAGTTGATAGGCGAAACGGTTTTGTTTTTCCGGTCGGTATGCCAATTGTTCAAGAATATT
TACCTGATGGTGCAGGTAAAACAATGGATTATGTCTATAAATCAGCTAAACAAGCGCTTACCTCAAAAGATATTGAATAT
ATCGCAACTTACTCAGGCCTGCCTGTTTATGTCAAAGGACCACAGTGTGCTGAGGATACCTTGCGGGCATTGGACGCAGG
AGCATCAGGTATCTGGGTAACCAATCATGGTGGCCGTCAATTAGATGGTGGTCCAGCTGCTTTTGATTCCTTACAAGAAG
TCGCTGAAGCAGTTGATCAAAAAGTTCCTATTGTCTTTGACTCAGGTATAAGACGGGGTCAACATATTTTCAAAGCTCTT
GCTTCAGGTGCTGACCTTGTTGCCTTGGGGCGTCCAGCGATTTACGGCTTGGCTATGGGAGGAAGCATTGGTACTCGTCA
GGTCTTTGAAAAGTTAAATGACGAGTTAAAAATGGTTATGCAACTGGCTGGGACACAAACTATCCAAGATGTTAAAGCCT
TTAACCTTCGCCACAATCCGTATGATTCATCGATTCCATTTGATCAAAATGCGCTACGCTTAGATTAG

Upstream 100 bases:

>100_bases
CAAGGAGGTGATCAGTGAGCATATCGTTTGACAGTTAAGAGTAGGATTACTATAATAGAAGCTTAAATGTAAGCGCTAAC
AAAATAAAAAATATAGGAGA

Downstream 100 bases:

>100_bases
AACAGACAAAAACCTCCGCTATCATGTCACAAATAGTAGAGGTTTTACACTGCAAAAAGAGGTATGAAAATCCAATAACA
TAGTTACAAGGTTTTCGTTT

Product: L-lactate oxidase

Products: NA

Alternate protein names: Lactate oxidase [H]

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MSMAQKTVITEETTDFVMDFKTSSAEGNVDFINVFDLEKMAQQVIPKGAFGYIASGAGDTFTLHENIRSFNHKLIVPHSL
KGVENPSTEITFDGDYLTSPLILAPVAAHKLANEQGEVASAKGLKEFGSIYTTSSYSTTDLPEISAALGGTPHWFQFYYS
KDDGINRNIMDRVKAQGCKAIVLTADATVGGNREVDRRNGFVFPVGMPIVQEYLPDGAGKTMDYVYKSAKQALTSKDIEY
IATYSGLPVYVKGPQCAEDTLRALDAGASGIWVTNHGGRQLDGGPAAFDSLQEVAEAVDQKVPIVFDSGIRRGQHIFKAL
ASGADLVALGRPAIYGLAMGGSIGTRQVFEKLNDELKMVMQLAGTQTIQDVKAFNLRHNPYDSSIPFDQNALRLD

Sequences:

>Translated_395_residues
MSMAQKTVITEETTDFVMDFKTSSAEGNVDFINVFDLEKMAQQVIPKGAFGYIASGAGDTFTLHENIRSFNHKLIVPHSL
KGVENPSTEITFDGDYLTSPLILAPVAAHKLANEQGEVASAKGLKEFGSIYTTSSYSTTDLPEISAALGGTPHWFQFYYS
KDDGINRNIMDRVKAQGCKAIVLTADATVGGNREVDRRNGFVFPVGMPIVQEYLPDGAGKTMDYVYKSAKQALTSKDIEY
IATYSGLPVYVKGPQCAEDTLRALDAGASGIWVTNHGGRQLDGGPAAFDSLQEVAEAVDQKVPIVFDSGIRRGQHIFKAL
ASGADLVALGRPAIYGLAMGGSIGTRQVFEKLNDELKMVMQLAGTQTIQDVKAFNLRHNPYDSSIPFDQNALRLD
>Mature_394_residues
SMAQKTVITEETTDFVMDFKTSSAEGNVDFINVFDLEKMAQQVIPKGAFGYIASGAGDTFTLHENIRSFNHKLIVPHSLK
GVENPSTEITFDGDYLTSPLILAPVAAHKLANEQGEVASAKGLKEFGSIYTTSSYSTTDLPEISAALGGTPHWFQFYYSK
DDGINRNIMDRVKAQGCKAIVLTADATVGGNREVDRRNGFVFPVGMPIVQEYLPDGAGKTMDYVYKSAKQALTSKDIEYI
ATYSGLPVYVKGPQCAEDTLRALDAGASGIWVTNHGGRQLDGGPAAFDSLQEVAEAVDQKVPIVFDSGIRRGQHIFKALA
SGADLVALGRPAIYGLAMGGSIGTRQVFEKLNDELKMVMQLAGTQTIQDVKAFNLRHNPYDSSIPFDQNALRLD

Specific function: Unknown

COG id: COG1304

COG function: function code C; L-lactate dehydrogenase (FMN-dependent) and related alpha-hydroxy acid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FMN hydroxy acid dehydrogenase domain [H]

Homologues:

Organism=Homo sapiens, GI11068137, Length=361, Percent_Identity=32.6869806094183, Blast_Score=214, Evalue=1e-55,
Organism=Homo sapiens, GI54234014, Length=343, Percent_Identity=31.4868804664723, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI7705393, Length=343, Percent_Identity=31.4868804664723, Blast_Score=197, Evalue=2e-50,
Organism=Escherichia coli, GI1790033, Length=364, Percent_Identity=31.5934065934066, Blast_Score=180, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI193208036, Length=354, Percent_Identity=37.2881355932203, Blast_Score=218, Evalue=4e-57,
Organism=Caenorhabditis elegans, GI32566217, Length=259, Percent_Identity=37.0656370656371, Blast_Score=150, Evalue=9e-37,
Organism=Saccharomyces cerevisiae, GI6323587, Length=358, Percent_Identity=32.4022346368715, Blast_Score=169, Evalue=5e-43,
Organism=Drosophila melanogaster, GI78707188, Length=357, Percent_Identity=35.2941176470588, Blast_Score=207, Evalue=9e-54,
Organism=Drosophila melanogaster, GI281363140, Length=357, Percent_Identity=35.2941176470588, Blast_Score=207, Evalue=1e-53,
Organism=Drosophila melanogaster, GI78707190, Length=357, Percent_Identity=35.2941176470588, Blast_Score=207, Evalue=1e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR012133
- InterPro:   IPR000262
- InterPro:   IPR008259 [H]

Pfam domain/function: PF01070 FMN_dh [H]

EC number: =1.13.12.4 [H]

Molecular weight: Translated: 42799; Mature: 42668

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMAQKTVITEETTDFVMDFKTSSAEGNVDFINVFDLEKMAQQVIPKGAFGYIASGAGDT
CCCCHHHEECCCCHHHEEEEECCCCCCCEEEEEEHHHHHHHHHHCCCCCCCEEECCCCCE
FTLHENIRSFNHKLIVPHSLKGVENPSTEITFDGDYLTSPLILAPVAAHKLANEQGEVAS
EEEHHHHHHCCCEEEECCCCCCCCCCCCEEEECCCHHCCCHHHHHHHHHHHCCCCCCHHH
AKGLKEFGSIYTTSSYSTTDLPEISAALGGTPHWFQFYYSKDDGINRNIMDRVKAQGCKA
HHHHHHHCCEEECCCCCCCCCCHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCE
IVLTADATVGGNREVDRRNGFVFPVGMPIVQEYLPDGAGKTMDYVYKSAKQALTSKDIEY
EEEEECCCCCCCCCCCCCCCEEEECCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCE
IATYSGLPVYVKGPQCAEDTLRALDAGASGIWVTNHGGRQLDGGPAAFDSLQEVAEAVDQ
EEECCCCEEEEECCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHC
KVPIVFDSGIRRGQHIFKALASGADLVALGRPAIYGLAMGGSIGTRQVFEKLNDELKMVM
CCCEEECCCCHHHHHHHHHHHCCCCEEEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHH
QLAGTQTIQDVKAFNLRHNPYDSSIPFDQNALRLD
HHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCEECC
>Mature Secondary Structure 
SMAQKTVITEETTDFVMDFKTSSAEGNVDFINVFDLEKMAQQVIPKGAFGYIASGAGDT
CCCHHHEECCCCHHHEEEEECCCCCCCEEEEEEHHHHHHHHHHCCCCCCCEEECCCCCE
FTLHENIRSFNHKLIVPHSLKGVENPSTEITFDGDYLTSPLILAPVAAHKLANEQGEVAS
EEEHHHHHHCCCEEEECCCCCCCCCCCCEEEECCCHHCCCHHHHHHHHHHHCCCCCCHHH
AKGLKEFGSIYTTSSYSTTDLPEISAALGGTPHWFQFYYSKDDGINRNIMDRVKAQGCKA
HHHHHHHCCEEECCCCCCCCCCHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCE
IVLTADATVGGNREVDRRNGFVFPVGMPIVQEYLPDGAGKTMDYVYKSAKQALTSKDIEY
EEEEECCCCCCCCCCCCCCCEEEECCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCE
IATYSGLPVYVKGPQCAEDTLRALDAGASGIWVTNHGGRQLDGGPAAFDSLQEVAEAVDQ
EEECCCCEEEEECCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHC
KVPIVFDSGIRRGQHIFKALASGADLVALGRPAIYGLAMGGSIGTRQVFEKLNDELKMVM
CCCEEECCCCHHHHHHHHHHHCCCCEEEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHH
QLAGTQTIQDVKAFNLRHNPYDSSIPFDQNALRLD
HHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2324094; 3571231 [H]