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The map label for this gene is ctfB [H]

Identifier: 15674353

GI number: 15674353

Start: 130794

End: 131441

Strand: Direct

Name: ctfB [H]

Synonym: SPy_0142

Alternate gene names: 15674353

Gene position: 130794-131441 (Clockwise)

Preceding gene: 15674352

Following gene: 15674355

Centisome position: 7.06

GC content: 39.97

Gene sequence:

>648_bases
ATGGCGGTTGCATTAAGTAAAGAAGAGATTCAAAATCGTATTGCAAAGCGTGTGGCCAAAGAATTAGAAGATGGTACTTT
GGTAAATTTAGGTATTGGTCTACCAACCAAAGTAGCAAATTTTGTGCCTGAAGAAATGACAGTGTATTTTCAATCTGAAA
ATGGGTTTATTGGACTAGGTCCTAAGTCAGATGATCCGAATTCGACTATCGTTAATGCAGGAGGACAGCCTGTGACGGTT
TATCCAGGAGCCGCTTTCTTTAATAGTGCAGATTCTTTTGGCATTATACGTGGTGGTCATGTTGATTTGACCGTCTTGGG
TGCTTTAGAAATTGCAGAAAATGGAGATATCGCCAATTACCTGATTCCAGGCAAAATGGTTCCTGGTATGGGAGGAGCGA
TGGATTTATTAGTTGGTGCTAAAAAAGTCATCGTAGCTATGGAGCATACCAATAAAGGAAAGCATAAGCTCCTAAAAGAA
TGTACCTTACCTTTAACAGCAAAAGGTGTGGTTGACCTTATTATCACAGAAATGGGTGTGTTTAAAGTAACGCCAGATGG
TATCCAAGTAATTGAAATCAGTGAAGGGTTTACTTTTGATGAGGTTCAAGCTGCGACAGGTGTACCTCTAAGCATAGCTG
AAGGTTAA

Upstream 100 bases:

>100_bases
TTCAGGCGCGAGAAATAGTACCAGTCGGCACCATACAACCAGAATGCGTTCATACACCACATATTTTTGTAGATTACATT
GTTAAAGAAGGAGTATAGTT

Downstream 100 bases:

>100_bases
TGACCAGTAGCCTTTTCTTGCCATAAAGGCATATTGAGTAAAAAAAACGTCAGTCATCGGTATATCAGAGATTGTGATGC
TTATCTGAAATACTTTTAAC

Product: butyrate-acetoacetate CoA-transferase subunit beta

Products: NA

Alternate protein names: Coat B; Acetoacetyl-CoA:acetate/butyrate:CoA transferase subunit B [H]

Number of amino acids: Translated: 215; Mature: 214

Protein sequence:

>215_residues
MAVALSKEEIQNRIAKRVAKELEDGTLVNLGIGLPTKVANFVPEEMTVYFQSENGFIGLGPKSDDPNSTIVNAGGQPVTV
YPGAAFFNSADSFGIIRGGHVDLTVLGALEIAENGDIANYLIPGKMVPGMGGAMDLLVGAKKVIVAMEHTNKGKHKLLKE
CTLPLTAKGVVDLIITEMGVFKVTPDGIQVIEISEGFTFDEVQAATGVPLSIAEG

Sequences:

>Translated_215_residues
MAVALSKEEIQNRIAKRVAKELEDGTLVNLGIGLPTKVANFVPEEMTVYFQSENGFIGLGPKSDDPNSTIVNAGGQPVTV
YPGAAFFNSADSFGIIRGGHVDLTVLGALEIAENGDIANYLIPGKMVPGMGGAMDLLVGAKKVIVAMEHTNKGKHKLLKE
CTLPLTAKGVVDLIITEMGVFKVTPDGIQVIEISEGFTFDEVQAATGVPLSIAEG
>Mature_214_residues
AVALSKEEIQNRIAKRVAKELEDGTLVNLGIGLPTKVANFVPEEMTVYFQSENGFIGLGPKSDDPNSTIVNAGGQPVTVY
PGAAFFNSADSFGIIRGGHVDLTVLGALEIAENGDIANYLIPGKMVPGMGGAMDLLVGAKKVIVAMEHTNKGKHKLLKEC
TLPLTAKGVVDLIITEMGVFKVTPDGIQVIEISEGFTFDEVQAATGVPLSIAEG

Specific function: Involved in solventogenic switch which allows C.acetobutylicum to uptake acid and produce solvents. Acts mainly to detoxify the medium by removing the acetate and butyrate excreted earlier in the fermentation [H]

COG id: COG2057

COG function: function code I; Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-oxoacid CoA-transferase subunit B family [H]

Homologues:

Organism=Homo sapiens, GI4557817, Length=211, Percent_Identity=51.6587677725119, Blast_Score=224, Evalue=4e-59,
Organism=Homo sapiens, GI11545841, Length=212, Percent_Identity=47.1698113207547, Blast_Score=202, Evalue=1e-52,
Organism=Escherichia coli, GI1788552, Length=206, Percent_Identity=52.4271844660194, Blast_Score=227, Evalue=4e-61,
Organism=Caenorhabditis elegans, GI17531517, Length=206, Percent_Identity=48.0582524271845, Blast_Score=194, Evalue=2e-50,
Organism=Drosophila melanogaster, GI21358325, Length=209, Percent_Identity=50.7177033492823, Blast_Score=216, Evalue=8e-57,
Organism=Drosophila melanogaster, GI24655794, Length=209, Percent_Identity=50.7177033492823, Blast_Score=216, Evalue=8e-57,

Paralogues:

None

Copy number: 369 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012791
- InterPro:   IPR004165
- InterPro:   IPR004164 [H]

Pfam domain/function: PF01144 CoA_trans [H]

EC number: =2.8.3.9 [H]

Molecular weight: Translated: 22581; Mature: 22450

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS01274 COA_TRANSF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVALSKEEIQNRIAKRVAKELEDGTLVNLGIGLPTKVANFVPEEMTVYFQSENGFIGLG
CCEECCHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHCCCCEEEEEEECCCCEEEEC
PKSDDPNSTIVNAGGQPVTVYPGAAFFNSADSFGIIRGGHVDLTVLGALEIAENGDIANY
CCCCCCCCEEEECCCCEEEECCCHHHHCCCCCCCEEECCCEEEEEEEEEEECCCCCEEEE
LIPGKMVPGMGGAMDLLVGAKKVIVAMEHTNKGKHKLLKECTLPLTAKGVVDLIITEMGV
EECCCCCCCCCCHHHHHHCCEEEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCE
FKVTPDGIQVIEISEGFTFDEVQAATGVPLSIAEG
EEECCCCEEEEEECCCCCHHHHHHHCCCCEEECCC
>Mature Secondary Structure 
AVALSKEEIQNRIAKRVAKELEDGTLVNLGIGLPTKVANFVPEEMTVYFQSENGFIGLG
CEECCHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHCCCCEEEEEEECCCCEEEEC
PKSDDPNSTIVNAGGQPVTVYPGAAFFNSADSFGIIRGGHVDLTVLGALEIAENGDIANY
CCCCCCCCEEEECCCCEEEECCCHHHHCCCCCCCEEECCCEEEEEEEEEEECCCCCEEEE
LIPGKMVPGMGGAMDLLVGAKKVIVAMEHTNKGKHKLLKECTLPLTAKGVVDLIITEMGV
EECCCCCCCCCCHHHHHHCCEEEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCE
FKVTPDGIQVIEISEGFTFDEVQAATGVPLSIAEG
EEECCCCEEEEEECCCCCHHHHHHHCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2254264; 8226639; 8423010; 11466286; 2383002 [H]