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The map label for this gene is pth

Identifier: 15674255

GI number: 15674255

Start: 4665

End: 5234

Strand: Direct

Name: pth

Synonym: SPy_0007

Alternate gene names: 15674255

Gene position: 4665-5234 (Clockwise)

Preceding gene: 15674254

Following gene: 15674256

Centisome position: 0.25

GC content: 31.75

Gene sequence:

>570_bases
ATGGTAAAAATGATTGTTGGTCTGGGAAATCCAGGCTCTAAATATGAAAAAACAAAGCACAATATTGGTTTTATGGCTAT
TGACAATATTGTCAAGAACCTTGACGTTACCTTTACAGATGATAAAAATTTTAAAGCACAGATAGGAAGTACTTTTATTA
ATCATGAAAAAGTTTACTTTGTGAAACCTACTACTTTTATGAATAATAGCGGCATAGCAGTAAAAGCATTACTAACCTAC
TATAATATTGACATAACAGATTTAATTGTTATCTATGATGATTTAGACATGGAAGTCAGTAAATTACGTTTACGTAGTAA
GGGTTCAGCAGGAGGACATAATGGCATTAAGTCAATCATTGCCCACATTGGAACTCAGGAATTTAACCGAATCAAAGTTG
GTATTGGACGACCTTTAAAAGGTATGACTGTTATTAACCATGTGATGGGCCAATTCAATACCGAAGATAATATTGCTATT
TCGTTAACTCTTGACAGAGTTGTCAATGCTGTCAAGTTTTATTTACAAGAAAATGATTTTGAAAAAACAATGCAGAAATT
TAATGGATAA

Upstream 100 bases:

>100_bases
GGACATCATGGAATTCAGATTTAATGTGTAATTATATTAAAACAATATCAGAAGGTTGGAAGAACATTCCAGCCCTTTTG
GCATTTTAGAAAGAGAAAAT

Downstream 100 bases:

>100_bases
TCATGGATATTTTAGAATTATTTAGTCAGAATAAGAAAGTCCAATCCTGGCACTCTGGATTAACCACCTTAGGAAGACAA
CTGGTAATGGGGTTATCGGG

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH

Number of amino acids: Translated: 189; Mature: 189

Protein sequence:

>189_residues
MVKMIVGLGNPGSKYEKTKHNIGFMAIDNIVKNLDVTFTDDKNFKAQIGSTFINHEKVYFVKPTTFMNNSGIAVKALLTY
YNIDITDLIVIYDDLDMEVSKLRLRSKGSAGGHNGIKSIIAHIGTQEFNRIKVGIGRPLKGMTVINHVMGQFNTEDNIAI
SLTLDRVVNAVKFYLQENDFEKTMQKFNG

Sequences:

>Translated_189_residues
MVKMIVGLGNPGSKYEKTKHNIGFMAIDNIVKNLDVTFTDDKNFKAQIGSTFINHEKVYFVKPTTFMNNSGIAVKALLTY
YNIDITDLIVIYDDLDMEVSKLRLRSKGSAGGHNGIKSIIAHIGTQEFNRIKVGIGRPLKGMTVINHVMGQFNTEDNIAI
SLTLDRVVNAVKFYLQENDFEKTMQKFNG
>Mature_189_residues
MVKMIVGLGNPGSKYEKTKHNIGFMAIDNIVKNLDVTFTDDKNFKAQIGSTFINHEKVYFVKPTTFMNNSGIAVKALLTY
YNIDITDLIVIYDDLDMEVSKLRLRSKGSAGGHNGIKSIIAHIGTQEFNRIKVGIGRPLKGMTVINHVMGQFNTEDNIAI
SLTLDRVVNAVKFYLQENDFEKTMQKFNG

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family

Homologues:

Organism=Homo sapiens, GI50897284, Length=178, Percent_Identity=28.6516853932584, Blast_Score=111, Evalue=4e-25,
Organism=Escherichia coli, GI1787455, Length=177, Percent_Identity=31.0734463276836, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTH_STRP1 (Q9A206)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_268428.1
- RefSeq:   YP_281369.1
- ProteinModelPortal:   Q9A206
- SMR:   Q9A206
- EnsemblBacteria:   EBSTRT00000000892
- EnsemblBacteria:   EBSTRT00000028230
- GeneID:   3571015
- GeneID:   900371
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_0007
- KEGG:   spz:M5005_Spy_0005
- GeneTree:   EBGT00050000028314
- HOGENOM:   HBG610927
- OMA:   AMHRLHS
- ProtClustDB:   PRK05426
- BioCyc:   SPYO160490:SPY0007-MONOMER
- BioCyc:   SPYO293653:M5005_SPY0005-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00083
- InterPro:   IPR001328
- InterPro:   IPR018171
- Gene3D:   G3DSA:3.40.50.1470
- PANTHER:   PTHR17224
- TIGRFAMs:   TIGR00447

Pfam domain/function: PF01195 Pept_tRNA_hydro; SSF53178 Pept_tRNA_hydro

EC number: =3.1.1.29

Molecular weight: Translated: 21197; Mature: 21197

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: PS01195 PEPT_TRNA_HYDROL_1; PS01196 PEPT_TRNA_HYDROL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKMIVGLGNPGSKYEKTKHNIGFMAIDNIVKNLDVTFTDDKNFKAQIGSTFINHEKVYF
CEEEEEEECCCCCCCHHHHHCCCCEEHHHHHHHCCEEEECCCCCHHHHCCEEECCCEEEE
VKPTTFMNNSGIAVKALLTYYNIDITDLIVIYDDLDMEVSKLRLRSKGSAGGHNGIKSII
ECCCEEECCCCCEEEEEEHHHCCCCEEEEEEEECCCCHHHHHHHHCCCCCCCHHHHHHHH
AHIGTQEFNRIKVGIGRPLKGMTVINHVMGQFNTEDNIAISLTLDRVVNAVKFYLQENDF
HHHCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHCCCCH
EKTMQKFNG
HHHHHHHCC
>Mature Secondary Structure
MVKMIVGLGNPGSKYEKTKHNIGFMAIDNIVKNLDVTFTDDKNFKAQIGSTFINHEKVYF
CEEEEEEECCCCCCCHHHHHCCCCEEHHHHHHHCCEEEECCCCCHHHHCCEEECCCEEEE
VKPTTFMNNSGIAVKALLTYYNIDITDLIVIYDDLDMEVSKLRLRSKGSAGGHNGIKSII
ECCCEEECCCCCEEEEEEHHHCCCCEEEEEEEECCCCHHHHHHHHCCCCCCCHHHHHHHH
AHIGTQEFNRIKVGIGRPLKGMTVINHVMGQFNTEDNIAISLTLDRVVNAVKFYLQENDF
HHHCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHCCCCH
EKTMQKFNG
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11296296