Click here to switch to the map view.

The map label for this gene is bioA [C]

Identifier: 13475002

GI number: 13475002

Start: 4843917

End: 4845293

Strand: Direct

Name: bioA [C]

Synonym: mlr5987

Alternate gene names: 13475002

Gene position: 4843917-4845293 (Clockwise)

Preceding gene: 13475001

Following gene: 13475003

Centisome position: 68.84

GC content: 63.25

Gene sequence:

>1377_bases
ATGAGGCACCAGATCCTGTCCAACCGTTCGGGCGAAGATCTGCGGGCCAGCGACCTCGCGCACCATCTGCATCCGTTCAC
AGACCATACCGTGCTGGCTGCAGAGGGTGGCCCTCGTGTCATCAAGGATGGCGACGGTATCTATGTTTGGGAAACGGAGG
GTCGGCGGTTGCTGGACGGGATGGCGGGATTGTGGTGCGTCAACGTCGGCTATGGCCGAAAGGAACTCGGCGAGGTCGCC
CGTCAGCAGATGGACCGGTTGGCTTTCTACAACACCTTCTTCAAGACCTCTCACGAGCCAGTGATCAATCTGGCGGAGAG
ACTCGCCGCCGTGACGCCAGAGGGCATTGACAACTTCTTTTTCGGCAATTCCGGTTCCGAAGCCAATGATACCGCGATCC
GGCTTGTGCGCAGCTATTGGGCCGCTGTGGAAAGGCCCGAGCGGCAGGTCGTGATCAGCCGAGAATATGCCTACCACGGC
TCTACCCTCGCAGGAGCAAGCCTTTGCGGCCTGCCGGGCATGCATGGCGTTGCGGGCCTTCCGATTCCCGGCATCGCGCA
CGCTGCCGCCCCGTATGCCTGGGCCGCTGGAAAGGACCAAGATGACCCGGCCTATGGTCGAGCATCTGCTGATGCGCTCG
AGGCGAAGATCTTGGAGATCGGTCCGAAGAACGTCGCCGCCTTCATCGGGGAGCCGGTGATGGGAGCCGGCGGGGTAATT
ACGCCGCCGCCGCGCTATTGGGCGCGGGTGCAGGAGATCTGCCGCAAGTACGACATCCTGTTAGTCTGCGACGAGGTGAT
CTGCGGCTTCGGGCGCACTGGGGAATGGTTCGGTGCTCAGAGCTTCGGCATCGCGCCAGACATTATGTGTATGGCGAAGG
GGCTTAGTTCTGGTTACATACCAATCTCGGCTGTCGGTGTCGGTACTCGCGTGGCCGAAATCTTGCGCACCCGCCCGGGC
GTCCTCTCGCACGGCTTCACCAGTTCCGGTCATCCGGTAGCTGCCGCCGTTGCACTGAAGAACATTGAACTCATCGAGGC
AGAAGGGTTGGTCAATCATGTACGCGACGTCGCCGGCCCGGCTCTCGCCCGCCGCCTGGGGGCGTTGGCAATGCACCCCC
TGGTTGGGCAGGTGCGCGGCATTGGCGCCATGTGGGCGCTGGAGCTGACGTCAGACAAGGCCACCCGTGCGCCCTTTGCG
CCGCTCGGCTCGGTCGCGACCCGCGTGCGCAATCTCTGCTTCGAACGGGGCCTGGCCACGCGGGCCGTTCGCGAGGGTCT
GGTGTTCGCACCGCCGCTGATCATCACCGAGGCGCAGGTGGACGAGATGGCGGACATTTTGACCTCCTGCCTTGACACGG
CCGTTGCGGAACTCTGA

Upstream 100 bases:

>100_bases
GACGCCTCGGCGTGAAGCATCGCCAAGCCTTAACCGGGTGGCCAACGCCCAAACACATTTCGGTCGGCCTTGGCAAGCAG
GCCCGGAAGGAGACGAGACG

Downstream 100 bases:

>100_bases
CGACGGATACGGAGAACTGACCTTGAAGATCGGTATCGCCCGCCTGTGGCACGAGGCCAATTCATTCACATCGACCAGCA
TCGGGTTAGGGCATTTCCAA

Product: putative aminotransferase

Products: S-Adenosyl-4-methylthio-2-oxobutanoate; 7,8-Diaminononanoate [C]

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MRHQILSNRSGEDLRASDLAHHLHPFTDHTVLAAEGGPRVIKDGDGIYVWETEGRRLLDGMAGLWCVNVGYGRKELGEVA
RQQMDRLAFYNTFFKTSHEPVINLAERLAAVTPEGIDNFFFGNSGSEANDTAIRLVRSYWAAVERPERQVVISREYAYHG
STLAGASLCGLPGMHGVAGLPIPGIAHAAAPYAWAAGKDQDDPAYGRASADALEAKILEIGPKNVAAFIGEPVMGAGGVI
TPPPRYWARVQEICRKYDILLVCDEVICGFGRTGEWFGAQSFGIAPDIMCMAKGLSSGYIPISAVGVGTRVAEILRTRPG
VLSHGFTSSGHPVAAAVALKNIELIEAEGLVNHVRDVAGPALARRLGALAMHPLVGQVRGIGAMWALELTSDKATRAPFA
PLGSVATRVRNLCFERGLATRAVREGLVFAPPLIITEAQVDEMADILTSCLDTAVAEL

Sequences:

>Translated_458_residues
MRHQILSNRSGEDLRASDLAHHLHPFTDHTVLAAEGGPRVIKDGDGIYVWETEGRRLLDGMAGLWCVNVGYGRKELGEVA
RQQMDRLAFYNTFFKTSHEPVINLAERLAAVTPEGIDNFFFGNSGSEANDTAIRLVRSYWAAVERPERQVVISREYAYHG
STLAGASLCGLPGMHGVAGLPIPGIAHAAAPYAWAAGKDQDDPAYGRASADALEAKILEIGPKNVAAFIGEPVMGAGGVI
TPPPRYWARVQEICRKYDILLVCDEVICGFGRTGEWFGAQSFGIAPDIMCMAKGLSSGYIPISAVGVGTRVAEILRTRPG
VLSHGFTSSGHPVAAAVALKNIELIEAEGLVNHVRDVAGPALARRLGALAMHPLVGQVRGIGAMWALELTSDKATRAPFA
PLGSVATRVRNLCFERGLATRAVREGLVFAPPLIITEAQVDEMADILTSCLDTAVAEL
>Mature_458_residues
MRHQILSNRSGEDLRASDLAHHLHPFTDHTVLAAEGGPRVIKDGDGIYVWETEGRRLLDGMAGLWCVNVGYGRKELGEVA
RQQMDRLAFYNTFFKTSHEPVINLAERLAAVTPEGIDNFFFGNSGSEANDTAIRLVRSYWAAVERPERQVVISREYAYHG
STLAGASLCGLPGMHGVAGLPIPGIAHAAAPYAWAAGKDQDDPAYGRASADALEAKILEIGPKNVAAFIGEPVMGAGGVI
TPPPRYWARVQEICRKYDILLVCDEVICGFGRTGEWFGAQSFGIAPDIMCMAKGLSSGYIPISAVGVGTRVAEILRTRPG
VLSHGFTSSGHPVAAAVALKNIELIEAEGLVNHVRDVAGPALARRLGALAMHPLVGQVRGIGAMWALELTSDKATRAPFA
PLGSVATRVRNLCFERGLATRAVREGLVFAPPLIITEAQVDEMADILTSCLDTAVAEL

Specific function: Biotin biosynthesis. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI13994255, Length=458, Percent_Identity=29.9126637554585, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI4557809, Length=440, Percent_Identity=29.3181818181818, Blast_Score=158, Evalue=9e-39,
Organism=Homo sapiens, GI24119277, Length=371, Percent_Identity=32.3450134770889, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI37574042, Length=419, Percent_Identity=31.0262529832936, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI226442705, Length=416, Percent_Identity=30.5288461538462, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI226442709, Length=384, Percent_Identity=30.9895833333333, Blast_Score=142, Evalue=8e-34,
Organism=Homo sapiens, GI284507298, Length=332, Percent_Identity=30.421686746988, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI188536080, Length=386, Percent_Identity=22.279792746114, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI38679950, Length=386, Percent_Identity=22.279792746114, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI38679946, Length=386, Percent_Identity=22.279792746114, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1786991, Length=455, Percent_Identity=29.8901098901099, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1789016, Length=412, Percent_Identity=30.0970873786408, Blast_Score=155, Evalue=7e-39,
Organism=Escherichia coli, GI1788044, Length=361, Percent_Identity=29.6398891966759, Blast_Score=145, Evalue=6e-36,
Organism=Escherichia coli, GI1789759, Length=403, Percent_Identity=27.2952853598015, Blast_Score=138, Evalue=6e-34,
Organism=Escherichia coli, GI1787560, Length=411, Percent_Identity=30.1703163017032, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI145693181, Length=357, Percent_Identity=28.2913165266106, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1786349, Length=369, Percent_Identity=25.7452574525745, Blast_Score=86, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI25144271, Length=418, Percent_Identity=30.3827751196172, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI71992977, Length=425, Percent_Identity=28, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI32564660, Length=439, Percent_Identity=28.7015945330296, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI25144274, Length=246, Percent_Identity=34.1463414634146, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17541228, Length=444, Percent_Identity=25.6756756756757, Blast_Score=89, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6323470, Length=467, Percent_Identity=26.9807280513919, Blast_Score=151, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6324386, Length=494, Percent_Identity=25.7085020242915, Blast_Score=137, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6324432, Length=415, Percent_Identity=24.3373493975904, Blast_Score=114, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6321456, Length=449, Percent_Identity=23.3853006681514, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28574759, Length=464, Percent_Identity=30.1724137931034, Blast_Score=151, Evalue=1e-36,
Organism=Drosophila melanogaster, GI21357415, Length=437, Percent_Identity=28.3752860411899, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI161085790, Length=404, Percent_Identity=31.1881188118812, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI21356575, Length=432, Percent_Identity=31.0185185185185, Blast_Score=144, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: 2.6.1.62 [C]

Molecular weight: Translated: 48995; Mature: 48995

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHQILSNRSGEDLRASDLAHHLHPFTDHTVLAAEGGPRVIKDGDGIYVWETEGRRLLDG
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCEEEECCCCEEEEECCCHHHHHH
MAGLWCVNVGYGRKELGEVARQQMDRLAFYNTFFKTSHEPVINLAERLAAVTPEGIDNFF
HCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCC
FGNSGSEANDTAIRLVRSYWAAVERPERQVVISREYAYHGSTLAGASLCGLPGMHGVAGL
CCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHCCCCCCCCHHCCCCCCCCCCCC
PIPGIAHAAAPYAWAAGKDQDDPAYGRASADALEAKILEIGPKNVAAFIGEPVMGAGGVI
CCCCHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCC
TPPPRYWARVQEICRKYDILLVCDEVICGFGRTGEWFGAQSFGIAPDIMCMAKGLSSGYI
CCCHHHHHHHHHHHHHCCEEEEEHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCC
PISAVGVGTRVAEILRTRPGVLSHGFTSSGHPVAAAVALKNIELIEAEGLVNHVRDVAGP
EEEEECHHHHHHHHHHCCCCCCCCCCCCCCCCCEEHHHHHCCCEEEHHHHHHHHHHHHHH
ALARRLGALAMHPLVGQVRGIGAMWALELTSDKATRAPFAPLGSVATRVRNLCFERGLAT
HHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCHHH
RAVREGLVFAPPLIITEAQVDEMADILTSCLDTAVAEL
HHHHCCCEECCCEEEEHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRHQILSNRSGEDLRASDLAHHLHPFTDHTVLAAEGGPRVIKDGDGIYVWETEGRRLLDG
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCEEEECCCCEEEEECCCHHHHHH
MAGLWCVNVGYGRKELGEVARQQMDRLAFYNTFFKTSHEPVINLAERLAAVTPEGIDNFF
HCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCC
FGNSGSEANDTAIRLVRSYWAAVERPERQVVISREYAYHGSTLAGASLCGLPGMHGVAGL
CCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHCCCCCCCCHHCCCCCCCCCCCC
PIPGIAHAAAPYAWAAGKDQDDPAYGRASADALEAKILEIGPKNVAAFIGEPVMGAGGVI
CCCCHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCC
TPPPRYWARVQEICRKYDILLVCDEVICGFGRTGEWFGAQSFGIAPDIMCMAKGLSSGYI
CCCHHHHHHHHHHHHHCCEEEEEHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCC
PISAVGVGTRVAEILRTRPGVLSHGFTSSGHPVAAAVALKNIELIEAEGLVNHVRDVAGP
EEEEECHHHHHHHHHHCCCCCCCCCCCCCCCCCEEHHHHHCCCEEEHHHHHHHHHHHHHH
ALARRLGALAMHPLVGQVRGIGAMWALELTSDKATRAPFAPLGSVATRVRNLCFERGLAT
HHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCHHH
RAVREGLVFAPPLIITEAQVDEMADILTSCLDTAVAEL
HHHHCCCEECCCEEEEHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal Phosphate. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 8-Amino-7-oxononanoate; S-Adenosyl-L-methionine [C]

Specific reaction: 8-Amino-7-oxononanoate + S-Adenosyl-L-methionine <==> S-Adenosyl-4-methylthio-2-oxobutanoate + 7,8-Diaminononanoate [C]

General reaction: Amino group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8796346; 9163424 [H]