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The map label for this gene is nifH [H]

Identifier: 13474919

GI number: 13474919

Start: 4771737

End: 4772630

Strand: Direct

Name: nifH [H]

Synonym: mlr5905

Alternate gene names: 13474919

Gene position: 4771737-4772630 (Clockwise)

Preceding gene: 13474917

Following gene: 13474920

Centisome position: 67.82

GC content: 60.51

Gene sequence:

>894_bases
ATGTCAGGTCTGCGTCAGATCGCATTCTACGGAAAGGGAGGCATCGGCAAGTCCACCACCTCTCAAAATACGCTAGCCGC
CCTGGTCGACCTCGGACAGAAAATCCTGATCGTCGGCTGCGACCCGAAAGCCGACTCCACCCGCCTGATCCTGAACGCAA
AGGCGCAGGATACGGTTCTGCATCTCGCCGCCCAGGAAGGTTCTGTGGAAGACCTCGAACTCCAGGACGTGCTCAAGATC
GGCTACAAAGACATCAAGTGTGTGGAGTCCGGCGGCCCCGAGCCGGGTGTCGGCTGCGCCGGCCGCGGCGTCATCACCTC
GATCAACTTCCTCGAGGAGAACGGCGCCTATGACAATGTCGACTATGTCTCCTACGACGTGCTGGGCGACGTGGTGTGCG
GCGGCTTCGCGATGCCGATCCGCGAAAACAAGGCCCAGGAAATCTACATCGTCATGTCCGGCGAGATGATGGCGCTCTAT
GCCGCCAACAACATCGCCAAGGGTATCCTGAAATACGCCCATTCGGGCGGCGTGAGGCTCGGGGGGCTGATCTGCAACGA
GCGCCAGACCGACCGCGAGCTCGACCTCTCCGAGGCGCTGGCCGCCAGACTCAATTCCAAGCTCATCCACTTCGTGCCGC
GCGACAACATCGTCCAGCACGCCGAACTGAGGAAGATGACGGTAATCCAGTATGCGCCGGACTCCAAACAGGCAGGGGAG
TACCGCGCGTTGGCCGAGAAGATCCATGGCAATTCGGGCCAGGGCACCATCCCGACCCCAATCACCATGGAGGAGCTCGA
GGACATGCTGCTCGACTTCGGCATCATGAAGACGGACGAGCAAATGCTTGCCGAGCTTCAGGCCAAGGAAGCGAAATTGG
CCGTCGCTCAGTAA

Upstream 100 bases:

>100_bases
TGGCACGAGTTTTGAAACGATCGGTGCGAGGCGGCGGGATCTGCCGACCGGCATTGAAGTCGCGGTAACCGCTATGGATG
GAAAGAAGGAAGGAAGCAAC

Downstream 100 bases:

>100_bases
CCGCCGCTACCGACAGGGAGGCGCCGACCGTGACCTGGCGCCCCTTTCTACGAAACAAGGCCTCGTTGGCGAGGCGTTAC
CCGAACCTTGAAAGGGGCAG

Product: nitrogenase reductase

Products: NA

Alternate protein names: Nitrogenase Fe protein; Nitrogenase component II; Nitrogenase reductase [H]

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MSGLRQIAFYGKGGIGKSTTSQNTLAALVDLGQKILIVGCDPKADSTRLILNAKAQDTVLHLAAQEGSVEDLELQDVLKI
GYKDIKCVESGGPEPGVGCAGRGVITSINFLEENGAYDNVDYVSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMALY
AANNIAKGILKYAHSGGVRLGGLICNERQTDRELDLSEALAARLNSKLIHFVPRDNIVQHAELRKMTVIQYAPDSKQAGE
YRALAEKIHGNSGQGTIPTPITMEELEDMLLDFGIMKTDEQMLAELQAKEAKLAVAQ

Sequences:

>Translated_297_residues
MSGLRQIAFYGKGGIGKSTTSQNTLAALVDLGQKILIVGCDPKADSTRLILNAKAQDTVLHLAAQEGSVEDLELQDVLKI
GYKDIKCVESGGPEPGVGCAGRGVITSINFLEENGAYDNVDYVSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMALY
AANNIAKGILKYAHSGGVRLGGLICNERQTDRELDLSEALAARLNSKLIHFVPRDNIVQHAELRKMTVIQYAPDSKQAGE
YRALAEKIHGNSGQGTIPTPITMEELEDMLLDFGIMKTDEQMLAELQAKEAKLAVAQ
>Mature_296_residues
SGLRQIAFYGKGGIGKSTTSQNTLAALVDLGQKILIVGCDPKADSTRLILNAKAQDTVLHLAAQEGSVEDLELQDVLKIG
YKDIKCVESGGPEPGVGCAGRGVITSINFLEENGAYDNVDYVSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMALYA
ANNIAKGILKYAHSGGVRLGGLICNERQTDRELDLSEALAARLNSKLIHFVPRDNIVQHAELRKMTVIQYAPDSKQAGEY
RALAEKIHGNSGQGTIPTPITMEELEDMLLDFGIMKTDEQMLAELQAKEAKLAVAQ

Specific function: The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components:the iron protein and the molybdenum-iron protein [H]

COG id: COG1348

COG function: function code P; Nitrogenase subunit NifH (ATPase)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NifH/BchL/ChlL family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000392
- InterPro:   IPR005977 [H]

Pfam domain/function: PF00142 Fer4_NifH [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 32062; Mature: 31931

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00746 NIFH_FRXC_1 ; PS00692 NIFH_FRXC_2 ; PS51026 NIFH_FRXC_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGLRQIAFYGKGGIGKSTTSQNTLAALVDLGQKILIVGCDPKADSTRLILNAKAQDTVL
CCCHHEEEEECCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCEEEEEECCCCCEEE
HLAAQEGSVEDLELQDVLKIGYKDIKCVESGGPEPGVGCAGRGVITSINFLEENGAYDNV
EEEECCCCCCCCCHHHHHHCCCHHEEEECCCCCCCCCCCCCCCCEEEEEEHHCCCCCCCC
DYVSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMALYAANNIAKGILKYAHSGGVRL
CEEEHHHHHHHHHCCEECCCCCCCCCEEEEEEECCEEEEEEHHHHHHHHHHHHHCCCCEE
GGLICNERQTDRELDLSEALAARLNSKLIHFVPRDNIVQHAELRKMTVIQYAPDSKQAGE
EEEEECCCCCCCCCCHHHHHHHHHCCCEEEEECCCCHHHHHHHHEEEEEEECCCCCCCHH
YRALAEKIHGNSGQGTIPTPITMEELEDMLLDFGIMKTDEQMLAELQAKEAKLAVAQ
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHEECCC
>Mature Secondary Structure 
SGLRQIAFYGKGGIGKSTTSQNTLAALVDLGQKILIVGCDPKADSTRLILNAKAQDTVL
CCHHEEEEECCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCEEEEEECCCCCEEE
HLAAQEGSVEDLELQDVLKIGYKDIKCVESGGPEPGVGCAGRGVITSINFLEENGAYDNV
EEEECCCCCCCCCHHHHHHCCCHHEEEECCCCCCCCCCCCCCCCEEEEEEHHCCCCCCCC
DYVSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMALYAANNIAKGILKYAHSGGVRL
CEEEHHHHHHHHHCCEECCCCCCCCCEEEEEEECCEEEEEEHHHHHHHHHHHHHCCCCEE
GGLICNERQTDRELDLSEALAARLNSKLIHFVPRDNIVQHAELRKMTVIQYAPDSKQAGE
EEEEECCCCCCCCCCHHHHHHHHHCCCEEEEECCCCHHHHHHHHEEEEEEECCCCCCCHH
YRALAEKIHGNSGQGTIPTPITMEELEDMLLDFGIMKTDEQMLAELQAKEAKLAVAQ
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2744485; 9163424 [H]