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The map label for this gene is nifD1 [H]

Identifier: 13474920

GI number: 13474920

Start: 4772736

End: 4774241

Strand: Direct

Name: nifD1 [H]

Synonym: mlr5906

Alternate gene names: 13474920

Gene position: 4772736-4774241 (Clockwise)

Preceding gene: 13474919

Following gene: 13474921

Centisome position: 67.83

GC content: 59.56

Gene sequence:

>1506_bases
ATGGGCCTCGACTATGAGAATGACGGCGCTTTGCATGCGAAGCTTATCGAAGACGTGCTGTCCCAATATCCAGACAAGGC
GGCGAAGCGTCGCAAGAAGCACCTCAGTGTCGCAACGAGCAAGGACGAGGCCGGCGGGGAGGACAAGGGCCTTTCCGAAT
GCGACGTCAAATCGAACATCAAATCCATTCCGGGCGTGATGACAATCCGCGGCTGCGCCTATGCCGGCTCCAAGGGCGTG
GTGTGGGGTCCAGTCAAGGATATGGTCCACATCTCGCACGGGCCGGTCGGCTGTGGGCAATATTCCTGGTCGCAACGCCG
CAACTATTACGTCGGCACGACGGGCATCGACACGTTCGTGACAATGCAGTTCACCTCCGACTTCCAGGAGAAGGACATCG
TTTTCGGCGGCGACAAGAAGCTGGAAAAGATCATCGACGAGATCGAGGACCTGTTTCCGCTCAGTGGCGGCATCTCGGTG
CAGTCCGAATGCCCGATCGGCCTGATCGGGGACGATATCGAGGCCGTGTCGCGCAAGAAAGCCAAGGAGCACGAAAAGAC
GATTGTACCGGTGCGCTGCGAAGGTTTCCGCGGCGTTTCACAATCGCTCGGCCACCACATTGCCAATGATGCGATCCGCG
ATTGGGTCTTCGACAAGGACGACGTCGCGTTCGAGTCCGGCCCATACGACGTCAACGTCGTCGGCGACTACAACATTGGC
GGCGATGCCTGGGCCTCGCGAATCCTGCTCGAGGAGATTGGGCTTCGGGTGGTCGGCAACTGGTCGGGCGACGCCACACT
CGCCGAGATAGAGCGCGCGCCCAAGGCCAAGCTCAACCTGATCCACTGCTACCGGTCGATGAACTACATCTGCCGGCACA
TGGAGGAAAAGTACGGCATCGCCTGGATGGAATACAATTTCTTCGGCCCCTCCCAGATCGAGGCCTCTCTGCGCAACATC
GCCAAGCATTTCGGCCCGGAAATCGAGGAGAAGACCGAAAAGGTCATCGCCAAATACCGGCCCTTGGTCGATGCCGTCAT
CGCCAAGTACCAACCGCGCCTCGAAGGCAGGACGGTGATGCTCTATGTCGGCGGGCTACGTCCTCGTCACGTCATCACTG
CTTACGAGGACCTCGCTATGGTGATCGTCGGCACCGGCTATGAGTTCGCCCACAACGACGACTATCAGCGCACCGGCCAT
TACGTGAAGAATGGCACGCTCCTCTATGACGACGTCACCGGTTATGAGCTGGAGAAGTTCATCGAAGGCATCCGCCCGGA
TCTCGTTGGCTCCGGTATCAAGGAGAAGTACCCGGTGCAGAAGATGGGCATCCCGTTCCGCCAGATGCACTCTTGGGACT
ACTCCGGCCCGTATCACGGTTATGACGGCTTCGCCATTTTCGCCCGCGACATGGATCTGGCCATCAACAACCCGGTCTGG
GGTCTCTACCGCGCGCCGTGGAAGAAGATGAAGGACGCGCCGCCGACGGCGGTCGCCGCCGAATGA

Upstream 100 bases:

>100_bases
GCTACCGACAGGGAGGCGCCGACCGTGACCTGGCGCCCCTTTCTACGAAACAAGGCCTCGTTGGCGAGGCGTTACCCGAA
CCTTGAAAGGGGCAGGTCCC

Downstream 100 bases:

>100_bases
GGACTGGCCTCCCTGGCAGGCAGGGAGGCCGATAAATGCTTGGGGCGTGCTCGATCCACGCTGGATGCCCTTCAAAGTTT
CGATGTCCCTGTGCTGCCGC

Product: nitrogenase molybdenum-iron protein alpha chain, nifD

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I [H]

Number of amino acids: Translated: 501; Mature: 500

Protein sequence:

>501_residues
MGLDYENDGALHAKLIEDVLSQYPDKAAKRRKKHLSVATSKDEAGGEDKGLSECDVKSNIKSIPGVMTIRGCAYAGSKGV
VWGPVKDMVHISHGPVGCGQYSWSQRRNYYVGTTGIDTFVTMQFTSDFQEKDIVFGGDKKLEKIIDEIEDLFPLSGGISV
QSECPIGLIGDDIEAVSRKKAKEHEKTIVPVRCEGFRGVSQSLGHHIANDAIRDWVFDKDDVAFESGPYDVNVVGDYNIG
GDAWASRILLEEIGLRVVGNWSGDATLAEIERAPKAKLNLIHCYRSMNYICRHMEEKYGIAWMEYNFFGPSQIEASLRNI
AKHFGPEIEEKTEKVIAKYRPLVDAVIAKYQPRLEGRTVMLYVGGLRPRHVITAYEDLAMVIVGTGYEFAHNDDYQRTGH
YVKNGTLLYDDVTGYELEKFIEGIRPDLVGSGIKEKYPVQKMGIPFRQMHSWDYSGPYHGYDGFAIFARDMDLAINNPVW
GLYRAPWKKMKDAPPTAVAAE

Sequences:

>Translated_501_residues
MGLDYENDGALHAKLIEDVLSQYPDKAAKRRKKHLSVATSKDEAGGEDKGLSECDVKSNIKSIPGVMTIRGCAYAGSKGV
VWGPVKDMVHISHGPVGCGQYSWSQRRNYYVGTTGIDTFVTMQFTSDFQEKDIVFGGDKKLEKIIDEIEDLFPLSGGISV
QSECPIGLIGDDIEAVSRKKAKEHEKTIVPVRCEGFRGVSQSLGHHIANDAIRDWVFDKDDVAFESGPYDVNVVGDYNIG
GDAWASRILLEEIGLRVVGNWSGDATLAEIERAPKAKLNLIHCYRSMNYICRHMEEKYGIAWMEYNFFGPSQIEASLRNI
AKHFGPEIEEKTEKVIAKYRPLVDAVIAKYQPRLEGRTVMLYVGGLRPRHVITAYEDLAMVIVGTGYEFAHNDDYQRTGH
YVKNGTLLYDDVTGYELEKFIEGIRPDLVGSGIKEKYPVQKMGIPFRQMHSWDYSGPYHGYDGFAIFARDMDLAINNPVW
GLYRAPWKKMKDAPPTAVAAE
>Mature_500_residues
GLDYENDGALHAKLIEDVLSQYPDKAAKRRKKHLSVATSKDEAGGEDKGLSECDVKSNIKSIPGVMTIRGCAYAGSKGVV
WGPVKDMVHISHGPVGCGQYSWSQRRNYYVGTTGIDTFVTMQFTSDFQEKDIVFGGDKKLEKIIDEIEDLFPLSGGISVQ
SECPIGLIGDDIEAVSRKKAKEHEKTIVPVRCEGFRGVSQSLGHHIANDAIRDWVFDKDDVAFESGPYDVNVVGDYNIGG
DAWASRILLEEIGLRVVGNWSGDATLAEIERAPKAKLNLIHCYRSMNYICRHMEEKYGIAWMEYNFFGPSQIEASLRNIA
KHFGPEIEEKTEKVIAKYRPLVDAVIAKYQPRLEGRTVMLYVGGLRPRHVITAYEDLAMVIVGTGYEFAHNDDYQRTGHY
VKNGTLLYDDVTGYELEKFIEGIRPDLVGSGIKEKYPVQKMGIPFRQMHSWDYSGPYHGYDGFAIFARDMDLAINNPVWG
LYRAPWKKMKDAPPTAVAAE

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR010143
- InterPro:   IPR000318
- InterPro:   IPR005972 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 56088; Mature: 55957

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00699 NITROGENASE_1_1 ; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLDYENDGALHAKLIEDVLSQYPDKAAKRRKKHLSVATSKDEAGGEDKGLSECDVKSNI
CCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCEEECCCCCCCCCCCCCCCCCHHHHH
KSIPGVMTIRGCAYAGSKGVVWGPVKDMVHISHGPVGCGQYSWSQRRNYYVGTTGIDTFV
HHCCCEEEEEEEEECCCCCCEECCHHHHEEECCCCCCCCCCCHHHCCCEEEECCCCCEEE
TMQFTSDFQEKDIVFGGDKKLEKIIDEIEDLFPLSGGISVQSECPIGLIGDDIEAVSRKK
EEEECCCCCCCCEEECCCHHHHHHHHHHHHHCCCCCCCEECCCCCEEEECCCHHHHHHHH
AKEHEKTIVPVRCEGFRGVSQSLGHHIANDAIRDWVFDKDDVAFESGPYDVNVVGDYNIG
HHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCC
GDAWASRILLEEIGLRVVGNWSGDATLAEIERAPKAKLNLIHCYRSMNYICRHMEEKYGI
HHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCE
AWMEYNFFGPSQIEASLRNIAKHFGPEIEEKTEKVIAKYRPLVDAVIAKYQPRLEGRTVM
EEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
LYVGGLRPRHVITAYEDLAMVIVGTGYEFAHNDDYQRTGHYVKNGTLLYDDVTGYELEKF
EEECCCCCCCEEEECCCEEEEEEECCEECCCCCCHHHCCCEEECCEEEEECCCCHHHHHH
IEGIRPDLVGSGIKEKYPVQKMGIPFRQMHSWDYSGPYHGYDGFAIFARDMDLAINNPVW
HHHCCHHHHCCCCCCCCCHHHHCCCHHHHCCCCCCCCCCCCCCEEEEEECCCEEECCCCC
GLYRAPWKKMKDAPPTAVAAE
CHHHCCHHHHCCCCCCCCCCC
>Mature Secondary Structure 
GLDYENDGALHAKLIEDVLSQYPDKAAKRRKKHLSVATSKDEAGGEDKGLSECDVKSNI
CCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCEEECCCCCCCCCCCCCCCCCHHHHH
KSIPGVMTIRGCAYAGSKGVVWGPVKDMVHISHGPVGCGQYSWSQRRNYYVGTTGIDTFV
HHCCCEEEEEEEEECCCCCCEECCHHHHEEECCCCCCCCCCCHHHCCCEEEECCCCCEEE
TMQFTSDFQEKDIVFGGDKKLEKIIDEIEDLFPLSGGISVQSECPIGLIGDDIEAVSRKK
EEEECCCCCCCCEEECCCHHHHHHHHHHHHHCCCCCCCEECCCCCEEEECCCHHHHHHHH
AKEHEKTIVPVRCEGFRGVSQSLGHHIANDAIRDWVFDKDDVAFESGPYDVNVVGDYNIG
HHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCC
GDAWASRILLEEIGLRVVGNWSGDATLAEIERAPKAKLNLIHCYRSMNYICRHMEEKYGI
HHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCE
AWMEYNFFGPSQIEASLRNIAKHFGPEIEEKTEKVIAKYRPLVDAVIAKYQPRLEGRTVM
EEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
LYVGGLRPRHVITAYEDLAMVIVGTGYEFAHNDDYQRTGHYVKNGTLLYDDVTGYELEKF
EEECCCCCCCEEEECCCEEEEEEECCEECCCCCCHHHCCCEEECCEEEEECCCCHHHHHH
IEGIRPDLVGSGIKEKYPVQKMGIPFRQMHSWDYSGPYHGYDGFAIFARDMDLAINNPVW
HHHCCHHHHCCCCCCCCCHHHHCCCHHHHCCCCCCCCCCCCCCEEEEEECCCEEECCCCC
GLYRAPWKKMKDAPPTAVAAE
CHHHCCHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424; 2744485 [H]