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The map label for this gene is fcl [H]

Identifier: 13474872

GI number: 13474872

Start: 4724785

End: 4725753

Strand: Direct

Name: fcl [H]

Synonym: mlr8749

Alternate gene names: 13474872

Gene position: 4724785-4725753 (Clockwise)

Preceding gene: 13474871

Following gene: 13474885

Centisome position: 67.15

GC content: 54.28

Gene sequence:

>969_bases
ATGGAAAACAAAAAGATGAAGAAAGTCTATGTGGCAGGCCATCGCGGCCTTGTTGGATCGGCCACGATGAGAGCCCTCGA
GGCGTTAGGATCCTATGAGATAATAACACGTACTCACGACGAACTGGATCTATTTGACCGGAGCGAGACGCGCAGGTTCT
TCATGTCGCAGCGGCCAGATTACGTCGTTATGTGCGCCGCGAAGGTTGGTGGTATACTGGCGAACGCCAGCTCCCCTGTC
GATTTCCTTCATAACAACCTCGCGATTCAAGTCAGTGTTTTTGATGCCGCTTACGCTTCCGGCGTCGAACGGATGATTTT
TCTTGGCTCCTCGTGTATCTATCCACGCGACTGTCCGCAGCCGATACGGGAGGAATACCTTCTCACTGGTCCGCTTGAAG
CGACGAATCGTCCCTACGCTTTGGCAAAAATCGCAGGTGTCGAATCGTGCTGGTCTTTCAACCGGCAGTACAAGGCGCGC
TATCTCGCGTTGATGCCGACCAATTTATATGGTCCCGGCGATAATTATCACCCCGAAAATTGTCACGTTCTGCCTGCTCT
GATACGCCGCTTTCATCAAGCTAAAATGAACGGCGACTCCTCTGTGGGGGTCTGGGGATCCGGAAATCCTCGGCGAGAGT
TTATGTATTCGTCGGATGTAGGCGATGCCATTGCGTTCCTGCTTGGCCTGCCAGATTCGGATTTTGACGCCTTGACAGCC
CCCGACACTGCGCCCCTGATTAATGTTGGCGTTGGTGAGGACGTCACAATTCGCGAGGTGGCGGAACTCGTCAAGGCGGC
GGTCGGCTGGGAGGGCAATTTGGTGTTCGACACGACGAAGCCAGACGGTACTCCGCGCAAGCTACTGGACGTAACGCGCT
TGCGTAACCTTGGCTGGAAAGCCAAAACGTCTCTTGGTGCCGGGTTGCAAGCGACGTATGAGGATTTTCTTCGCCTTCAT
GCGGCTTGA

Upstream 100 bases:

>100_bases
ATTCACACAGTCCACGAGAGTTATGCTGACAACGGCGGCGCCATGCGGGGTTGTTGTCAGTGCTATGTGGCCTTCTTGAG
GAGCGCCCAGAGCGTTAACA

Downstream 100 bases:

>100_bases
TGTGGAAGCGCGCATCCAGGTTGGACTGCATGTCCGACCGGGTGCAAGCTCGCCCAGTCCGGCCAAACAGATGAGAGGGA
CGCATACATCTCGAGGCGGC

Product: GDP-L-fucose synthetase(nodulation protein NolK)

Products: NA

Alternate protein names: GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MENKKMKKVYVAGHRGLVGSATMRALEALGSYEIITRTHDELDLFDRSETRRFFMSQRPDYVVMCAAKVGGILANASSPV
DFLHNNLAIQVSVFDAAYASGVERMIFLGSSCIYPRDCPQPIREEYLLTGPLEATNRPYALAKIAGVESCWSFNRQYKAR
YLALMPTNLYGPGDNYHPENCHVLPALIRRFHQAKMNGDSSVGVWGSGNPRREFMYSSDVGDAIAFLLGLPDSDFDALTA
PDTAPLINVGVGEDVTIREVAELVKAAVGWEGNLVFDTTKPDGTPRKLLDVTRLRNLGWKAKTSLGAGLQATYEDFLRLH
AA

Sequences:

>Translated_322_residues
MENKKMKKVYVAGHRGLVGSATMRALEALGSYEIITRTHDELDLFDRSETRRFFMSQRPDYVVMCAAKVGGILANASSPV
DFLHNNLAIQVSVFDAAYASGVERMIFLGSSCIYPRDCPQPIREEYLLTGPLEATNRPYALAKIAGVESCWSFNRQYKAR
YLALMPTNLYGPGDNYHPENCHVLPALIRRFHQAKMNGDSSVGVWGSGNPRREFMYSSDVGDAIAFLLGLPDSDFDALTA
PDTAPLINVGVGEDVTIREVAELVKAAVGWEGNLVFDTTKPDGTPRKLLDVTRLRNLGWKAKTSLGAGLQATYEDFLRLH
AA
>Mature_322_residues
MENKKMKKVYVAGHRGLVGSATMRALEALGSYEIITRTHDELDLFDRSETRRFFMSQRPDYVVMCAAKVGGILANASSPV
DFLHNNLAIQVSVFDAAYASGVERMIFLGSSCIYPRDCPQPIREEYLLTGPLEATNRPYALAKIAGVESCWSFNRQYKAR
YLALMPTNLYGPGDNYHPENCHVLPALIRRFHQAKMNGDSSVGVWGSGNPRREFMYSSDVGDAIAFLLGLPDSDFDALTA
PDTAPLINVGVGEDVTIREVAELVKAAVGWEGNLVFDTTKPDGTPRKLLDVTRLRNLGWKAKTSLGAGLQATYEDFLRLH
AA

Specific function: Two step NADP-dependent conversion of GDP-4-dehydro-6- deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fucose synthase family [H]

Homologues:

Organism=Homo sapiens, GI4507709, Length=292, Percent_Identity=33.5616438356164, Blast_Score=171, Evalue=9e-43,
Organism=Escherichia coli, GI1788365, Length=311, Percent_Identity=51.7684887459807, Blast_Score=335, Evalue=3e-93,
Organism=Caenorhabditis elegans, GI17554428, Length=297, Percent_Identity=31.3131313131313, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI19922778, Length=304, Percent_Identity=32.8947368421053, Blast_Score=157, Evalue=9e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.271 [H]

Molecular weight: Translated: 35544; Mature: 35544

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENKKMKKVYVAGHRGLVGSATMRALEALGSYEIITRTHDELDLFDRSETRRFFMSQRPD
CCCCCEEEEEEECCCCCCHHHHHHHHHHHCCEEEEECCCCHHHHHCCHHHHHHHHHCCCC
YVVMCAAKVGGILANASSPVDFLHNNLAIQVSVFDAAYASGVERMIFLGSSCIYPRDCPQ
EEEEEHHHHCCEEECCCCCHHHHHCCEEEEEEEEHHHHHHHHHEEEHCCCCCCCCCCCCC
PIREEYLLTGPLEATNRPYALAKIAGVESCWSFNRQYKARYLALMPTNLYGPGDNYHPEN
HHHHCEEEECCCCCCCCCEEHHHHHHHHHHHHHCHHHHEEEEEEECCCCCCCCCCCCCCC
CHVLPALIRRFHQAKMNGDSSVGVWGSGNPRREFMYSSDVGDAIAFLLGLPDSDFDALTA
CCHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHEEECCCHHHHHHHHHCCCCCCCCCCCC
PDTAPLINVGVGEDVTIREVAELVKAAVGWEGNLVFDTTKPDGTPRKLLDVTRLRNLGWK
CCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCC
AKTSLGAGLQATYEDFLRLHAA
HHHCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MENKKMKKVYVAGHRGLVGSATMRALEALGSYEIITRTHDELDLFDRSETRRFFMSQRPD
CCCCCEEEEEEECCCCCCHHHHHHHHHHHCCEEEEECCCCHHHHHCCHHHHHHHHHCCCC
YVVMCAAKVGGILANASSPVDFLHNNLAIQVSVFDAAYASGVERMIFLGSSCIYPRDCPQ
EEEEEHHHHCCEEECCCCCHHHHHCCEEEEEEEEHHHHHHHHHEEEHCCCCCCCCCCCCC
PIREEYLLTGPLEATNRPYALAKIAGVESCWSFNRQYKARYLALMPTNLYGPGDNYHPEN
HHHHCEEEECCCCCCCCCEEHHHHHHHHHHHHHCHHHHEEEEEEECCCCCCCCCCCCCCC
CHVLPALIRRFHQAKMNGDSSVGVWGSGNPRREFMYSSDVGDAIAFLLGLPDSDFDALTA
CCHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHEEECCCHHHHHHHHHCCCCCCCCCCCC
PDTAPLINVGVGEDVTIREVAELVKAAVGWEGNLVFDTTKPDGTPRKLLDVTRLRNLGWK
CCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCC
AKTSLGAGLQATYEDFLRLHAA
HHHCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7815923; 8759852; 9097040; 9278503; 9473059; 9862812 [H]