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The map label for this gene is rfbD [H]

Identifier: 13474871

GI number: 13474871

Start: 4723613

End: 4724713

Strand: Direct

Name: rfbD [H]

Synonym: mlr5849

Alternate gene names: 13474871

Gene position: 4723613-4724713 (Clockwise)

Preceding gene: 13474870

Following gene: 13474872

Centisome position: 67.13

GC content: 51.95

Gene sequence:

>1101_bases
TTGTCAAAGAGAAAAGTAGCTTTAATCACCGGGGTTACGGGACAAGACGGTTCCTACCTCGCAGAATTGCTTTTAGAAAA
GGGCTATTCTGTGCATGGGATAAAGAGACGATCGTCTCTTTTTAATACTGGCCGTATAGACCATCTCTATCATGACCCTC
ATGAAAGTGGCGTTGATCTTACACTTCACCACGGGGACTTGACAGACTCGTCGAGCCTTACGCGCGTCATCCAACTGGTT
CAGCCGGACGAAATTTACAATTTGGCAGCTCAGAGCCACGTTGCAGTCTCCTTTGAAGAACCAGAATACACCGCGAACTC
CGATGCCTTGGGTGCGCTGCGTATCCTCGAGGCAATTCGGATCCTGGGGCTCGTCAAGCATACGCGCTACTACCAAGCCT
CGACGTCTGAGCTCTACGGGCTGGTGCGGGAAACGCCACAGACCGAGACGACACCGTTCTACCCCCGGTCCCCCTACGCT
GTTGCCAAATTGTATGCTCACTGGATCACCGTAAATTATCGAGAATCTTACAATTTGTATGCTTGTAACGGCATTTTATT
TAATCATGAGTCACCTGCGCGCGGAGAAACATTTGTAACTCGCAAAATCACGCGCGCCTTGACTCGGATTAAGCTTGGAA
TGCAACGCACTCTATTCCTCGGCAACCTTAATGCGCGTCGCGATTGGGGGCACGCTCGAGACTACGTCCAGATGCAGTGG
TTGATGCTGCAGCAGCAGCAACCTGAAGACTTTGTGATCGCTAGCGGTGAGCAACATTCGGTGCGCGAATTCGTCACCGT
CGCGGCCGCTGAACTCGGCATCGATGTTCGTTGGGTGGGGGAAGGGGTCGACGAAGAGGGGTACGATGCAAAGACGGGAG
CTCTGATCGTAAAAGTAGATCCCCGCTATTTTCGTCCTGCAGAAGTCGAGACACTTCTCGGCGATGCCCGCAAGGCGAAA
GAAAAGCTTGGGTGGGAGCCCAAAATCTCCTTCATCGAATTGGTTAGGGAGATGGTTCGGGAAGATTTGCGCATGGCGGA
GCGTGAAGCAGTGCTGATGAATCAGGGTTATTATTCACACAGTCCACGAGAGTTATGCTGA

Upstream 100 bases:

>100_bases
AGGCCCCCGTTCGCCCTGCCCAATACGCCCCTTTCTCGCCACGCAATCCCGCCGCAGGCGAGCGTTTTCATGGGCGCGTC
AAAATCAAAAGGATTCAAGC

Downstream 100 bases:

>100_bases
CAACGGCGGCGCCATGCGGGGTTGTTGTCAGTGCTATGTGGCCTTCTTGAGGAGCGCCCAGAGCGTTAACAATGGAAAAC
AAAAAGATGAAGAAAGTCTA

Product: GDP-mannose 4,6-dehydratase (nodulation protein NoeL)

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 366; Mature: 365

Protein sequence:

>366_residues
MSKRKVALITGVTGQDGSYLAELLLEKGYSVHGIKRRSSLFNTGRIDHLYHDPHESGVDLTLHHGDLTDSSSLTRVIQLV
QPDEIYNLAAQSHVAVSFEEPEYTANSDALGALRILEAIRILGLVKHTRYYQASTSELYGLVRETPQTETTPFYPRSPYA
VAKLYAHWITVNYRESYNLYACNGILFNHESPARGETFVTRKITRALTRIKLGMQRTLFLGNLNARRDWGHARDYVQMQW
LMLQQQQPEDFVIASGEQHSVREFVTVAAAELGIDVRWVGEGVDEEGYDAKTGALIVKVDPRYFRPAEVETLLGDARKAK
EKLGWEPKISFIELVREMVREDLRMAEREAVLMNQGYYSHSPRELC

Sequences:

>Translated_366_residues
MSKRKVALITGVTGQDGSYLAELLLEKGYSVHGIKRRSSLFNTGRIDHLYHDPHESGVDLTLHHGDLTDSSSLTRVIQLV
QPDEIYNLAAQSHVAVSFEEPEYTANSDALGALRILEAIRILGLVKHTRYYQASTSELYGLVRETPQTETTPFYPRSPYA
VAKLYAHWITVNYRESYNLYACNGILFNHESPARGETFVTRKITRALTRIKLGMQRTLFLGNLNARRDWGHARDYVQMQW
LMLQQQQPEDFVIASGEQHSVREFVTVAAAELGIDVRWVGEGVDEEGYDAKTGALIVKVDPRYFRPAEVETLLGDARKAK
EKLGWEPKISFIELVREMVREDLRMAEREAVLMNQGYYSHSPRELC
>Mature_365_residues
SKRKVALITGVTGQDGSYLAELLLEKGYSVHGIKRRSSLFNTGRIDHLYHDPHESGVDLTLHHGDLTDSSSLTRVIQLVQ
PDEIYNLAAQSHVAVSFEEPEYTANSDALGALRILEAIRILGLVKHTRYYQASTSELYGLVRETPQTETTPFYPRSPYAV
AKLYAHWITVNYRESYNLYACNGILFNHESPARGETFVTRKITRALTRIKLGMQRTLFLGNLNARRDWGHARDYVQMQWL
MLQQQQPEDFVIASGEQHSVREFVTVAAAELGIDVRWVGEGVDEEGYDAKTGALIVKVDPRYFRPAEVETLLGDARKAKE
KLGWEPKISFIELVREMVREDLRMAEREAVLMNQGYYSHSPRELC

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=344, Percent_Identity=61.046511627907, Blast_Score=446, Evalue=1e-125,
Organism=Homo sapiens, GI42516563, Length=337, Percent_Identity=24.0356083086053, Blast_Score=65, Evalue=1e-10,
Organism=Escherichia coli, GI1788366, Length=363, Percent_Identity=68.870523415978, Blast_Score=528, Evalue=1e-151,
Organism=Escherichia coli, GI48994969, Length=350, Percent_Identity=25.4285714285714, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1788353, Length=355, Percent_Identity=23.3802816901408, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI133901790, Length=342, Percent_Identity=63.7426900584795, Blast_Score=464, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI17539422, Length=342, Percent_Identity=63.7426900584795, Blast_Score=464, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI17539424, Length=342, Percent_Identity=63.7426900584795, Blast_Score=464, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI133901788, Length=342, Percent_Identity=63.7426900584795, Blast_Score=464, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI133901786, Length=342, Percent_Identity=63.7426900584795, Blast_Score=464, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI17507723, Length=347, Percent_Identity=63.1123919308357, Blast_Score=462, Evalue=1e-130,
Organism=Drosophila melanogaster, GI24158427, Length=351, Percent_Identity=60.1139601139601, Blast_Score=459, Evalue=1e-129,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 41679; Mature: 41548

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKRKVALITGVTGQDGSYLAELLLEKGYSVHGIKRRSSLFNTGRIDHLYHDPHESGVDL
CCCCEEEEEEECCCCCHHHHHHHHHHCCCCEECCHHHHHHHCCCCCCCEECCCCCCCCEE
TLHHGDLTDSSSLTRVIQLVQPDEIYNLAAQSHVAVSFEEPEYTANSDALGALRILEAIR
EEECCCCCCCHHHHHHHHHCCCHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHHHHHH
ILGLVKHTRYYQASTSELYGLVRETPQTETTPFYPRSPYAVAKLYAHWITVNYRESYNLY
HHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHEEEEEECCCCEE
ACNGILFNHESPARGETFVTRKITRALTRIKLGMQRTLFLGNLNARRDWGHARDYVQMQW
EEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCHHHEEEEECCCCCCCCCHHHHHHHHHH
LMLQQQQPEDFVIASGEQHSVREFVTVAAAELGIDVRWVGEGVDEEGYDAKTGALIVKVD
HHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCEEEEEEC
PRYFRPAEVETLLGDARKAKEKLGWEPKISFIELVREMVREDLRMAEREAVLMNQGYYSH
CCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCC
SPRELC
CCCCCC
>Mature Secondary Structure 
SKRKVALITGVTGQDGSYLAELLLEKGYSVHGIKRRSSLFNTGRIDHLYHDPHESGVDL
CCCEEEEEEECCCCCHHHHHHHHHHCCCCEECCHHHHHHHCCCCCCCEECCCCCCCCEE
TLHHGDLTDSSSLTRVIQLVQPDEIYNLAAQSHVAVSFEEPEYTANSDALGALRILEAIR
EEECCCCCCCHHHHHHHHHCCCHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHHHHHH
ILGLVKHTRYYQASTSELYGLVRETPQTETTPFYPRSPYAVAKLYAHWITVNYRESYNLY
HHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHEEEEEECCCCEE
ACNGILFNHESPARGETFVTRKITRALTRIKLGMQRTLFLGNLNARRDWGHARDYVQMQW
EEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCHHHEEEEECCCCCCCCCHHHHHHHHHH
LMLQQQQPEDFVIASGEQHSVREFVTVAAAELGIDVRWVGEGVDEEGYDAKTGALIVKVD
HHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCEEEEEEC
PRYFRPAEVETLLGDARKAKEKLGWEPKISFIELVREMVREDLRMAEREAVLMNQGYYSH
CCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCC
SPRELC
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1372980; 7540582; 10952301 [H]