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The map label for this gene is nifA [H]

Identifier: 13474815

GI number: 13474815

Start: 4665317

End: 4665805

Strand: Direct

Name: nifA [H]

Synonym: mlr5785

Alternate gene names: 13474815

Gene position: 4665317-4665805 (Clockwise)

Preceding gene: 13474812

Following gene: 13474816

Centisome position: 66.31

GC content: 63.39

Gene sequence:

>489_bases
GTGGACCCGGCTTTCAACGCCGCCGACAAGGACGTGCTCGGGGCGTCCGACAGCATGAAAGTCTCGTTCATCGGCGTACC
CGTTCGCGTCGATGCGAACGTCGTGGGCACGCCGACCATCGACCGCATCTTGGACAACAAATCAAGTTCCCTCCTCGATT
ACGACCTGCGCCTCCTCACCACAATCGCCAATCTGGTTGGGCAGACCGTGAAGCTGCATCGCATGTTCCTGGCAGCGGGA
GCGTCAATCGGCAGAATGCCACGGACAGTGTTTTTTCGCGGCGCCGTGGAAGAGCGGACCGGGCAACAAGGTGCCGCAAC
TGGGCCGATGATGCCAGACAGGCCGGCCACGCGGCCGACTGAGGCTGCTCCTTGGGCCGCCGCTACGGTCCCGTCCGTCG
TCAGCGACCAGGCACCGCTGGCGCCTCCCAGCGCAGCCTCTTTAAAGGTACGAGTATGTCAAATCGCGAGCGTGTCATCG
CGGCCGTGA

Upstream 100 bases:

>100_bases
CATCGCACCGCTGTGCACTCGACCACGTTGCAGCGTCCCGACTTACGGCTAAGGCGTTTTTCGCGCTTGCCCGTGACCAG
TCAAAATCCGCAACCAAGCG

Downstream 100 bases:

>100_bases
AGAGATGGTTCGGTGCAGACAAAGGCGGCGCCTCCTTGGACTGACGCCGCGCCAGATTGGATACACGCTGAGGAAATACG
GCATCAGATCAAGCGTCCTT

Product: NIFA, nif-specific regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MDPAFNAADKDVLGASDSMKVSFIGVPVRVDANVVGTPTIDRILDNKSSSLLDYDLRLLTTIANLVGQTVKLHRMFLAAG
ASIGRMPRTVFFRGAVEERTGQQGAATGPMMPDRPATRPTEAAPWAAATVPSVVSDQAPLAPPSAASLKVRVCQIASVSS
RP

Sequences:

>Translated_162_residues
MDPAFNAADKDVLGASDSMKVSFIGVPVRVDANVVGTPTIDRILDNKSSSLLDYDLRLLTTIANLVGQTVKLHRMFLAAG
ASIGRMPRTVFFRGAVEERTGQQGAATGPMMPDRPATRPTEAAPWAAATVPSVVSDQAPLAPPSAASLKVRVCQIASVSS
RP
>Mature_162_residues
MDPAFNAADKDVLGASDSMKVSFIGVPVRVDANVVGTPTIDRILDNKSSSLLDYDLRLLTTIANLVGQTVKLHRMFLAAG
ASIGRMPRTVFFRGAVEERTGQQGAATGPMMPDRPATRPTEAAPWAAATVPSVVSDQAPLAPPSAASLKVRVCQIASVSS
RP

Specific function: Required for activation of most nif operons, which are directly involved in nitrogen fixation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR010113
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 17015; Mature: 17015

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPAFNAADKDVLGASDSMKVSFIGVPVRVDANVVGTPTIDRILDNKSSSLLDYDLRLLT
CCCCCCCCCCCCCCCCCCCEEEEEECEEEECCCCCCCCHHHHHHCCCCCCHHHHHHHHHH
TIANLVGQTVKLHRMFLAAGASIGRMPRTVFFRGAVEERTGQQGAATGPMMPDRPATRPT
HHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHCCCCCCCCCCCCCCCCCCCCC
EAAPWAAATVPSVVSDQAPLAPPSAASLKVRVCQIASVSSRP
CCCCCCHHHCCHHHCCCCCCCCCCCCCEEEEEEEEECCCCCC
>Mature Secondary Structure
MDPAFNAADKDVLGASDSMKVSFIGVPVRVDANVVGTPTIDRILDNKSSSLLDYDLRLLT
CCCCCCCCCCCCCCCCCCCEEEEEECEEEECCCCCCCCHHHHHHCCCCCCHHHHHHHHHH
TIANLVGQTVKLHRMFLAAGASIGRMPRTVFFRGAVEERTGQQGAATGPMMPDRPATRPT
HHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHCCCCCCCCCCCCCCCCCCCCC
EAAPWAAATVPSVVSDQAPLAPPSAASLKVRVCQIASVSSRP
CCCCCCHHHCCHHHCCCCCCCCCCCCCEEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3313281; 12597275; 3357773; 2792368 [H]