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The map label for this gene is mscL [H]

Identifier: 13474738

GI number: 13474738

Start: 4580297

End: 4580770

Strand: Direct

Name: mscL [H]

Synonym: mlr5692

Alternate gene names: 13474738

Gene position: 4580297-4580770 (Clockwise)

Preceding gene: 13474736

Following gene: 13474739

Centisome position: 65.1

GC content: 57.81

Gene sequence:

>474_bases
ATGCCGCAAGGACGGAACGGCAGCGCGGCGGGTCATGACAAGGAGGGTCGAATGCTGAAAGAATTCCAGGAATTCATTTC
CAAGGGTAACGTGATGGACCTTGCGGTCGGCGTCATCATCGGCGCGGCCTTCGGCAAGATCGTCACTTCGCTGGTCGACG
ACGTCATCATGCCAATCTTCGGTGCGATTTTCGGCGGTCTGGATTTCAATAACTATTACATAGGGCTGTCCTCGGCCGTC
AACGCTACCTCCCTCGCTGAGGCCAAGAAACAAGGCGCTGTTTTTGCCTATGGCAGCTTTATCACCGCAGTGCTGAACTT
CCTCATTCTCGCTTTCATCATCTTCCTGATGGTCAAGGCGGTGAACAATCTGCGCCGGCGGCTTGAGCGCGAGAAGCCGG
CTGCCCCGGCCGCACCACCCCCCGCCGATGTCGCGCTCCTCACCGAAATCCGCGATCTGCTCGCCAAGCGATAA

Upstream 100 bases:

>100_bases
GGAGGAAGTTCGCATTGCCGTACCGGAAGGAGAAAGCGGTTGCTTTCTCCTTTTTGCCCATGTTGCAACTTCAGGTTTTT
GTTATGTTGTCGCCGCATCG

Downstream 100 bases:

>100_bases
TTCAGCCAGCCATTGCTGGACCAAAACCCCGGTCGTTCGCGGCTGGGGTTTTCTGTTTTCGTATCTGGTAAGAGCGGCAA
GCCTAGAGCAATTCCAGGAA

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 157; Mature: 156

Protein sequence:

>157_residues
MPQGRNGSAAGHDKEGRMLKEFQEFISKGNVMDLAVGVIIGAAFGKIVTSLVDDVIMPIFGAIFGGLDFNNYYIGLSSAV
NATSLAEAKKQGAVFAYGSFITAVLNFLILAFIIFLMVKAVNNLRRRLEREKPAAPAAPPPADVALLTEIRDLLAKR

Sequences:

>Translated_157_residues
MPQGRNGSAAGHDKEGRMLKEFQEFISKGNVMDLAVGVIIGAAFGKIVTSLVDDVIMPIFGAIFGGLDFNNYYIGLSSAV
NATSLAEAKKQGAVFAYGSFITAVLNFLILAFIIFLMVKAVNNLRRRLEREKPAAPAAPPPADVALLTEIRDLLAKR
>Mature_156_residues
PQGRNGSAAGHDKEGRMLKEFQEFISKGNVMDLAVGVIIGAAFGKIVTSLVDDVIMPIFGAIFGGLDFNNYYIGLSSAVN
ATSLAEAKKQGAVFAYGSFITAVLNFLILAFIIFLMVKAVNNLRRRLEREKPAAPAAPPPADVALLTEIRDLLAKR

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell [H]

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family [H]

Homologues:

Organism=Escherichia coli, GI1789686, Length=140, Percent_Identity=50.7142857142857, Blast_Score=126, Evalue=6e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019823
- InterPro:   IPR001185
- InterPro:   IPR015089 [H]

Pfam domain/function: PF01741 MscL [H]

EC number: NA

Molecular weight: Translated: 16857; Mature: 16726

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQGRNGSAAGHDKEGRMLKEFQEFISKGNVMDLAVGVIIGAAFGKIVTSLVDDVIMPIF
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAIFGGLDFNNYYIGLSSAVNATSLAEAKKQGAVFAYGSFITAVLNFLILAFIIFLMVKA
HHHHCCCCCCCEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VNNLRRRLEREKPAAPAAPPPADVALLTEIRDLLAKR
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PQGRNGSAAGHDKEGRMLKEFQEFISKGNVMDLAVGVIIGAAFGKIVTSLVDDVIMPIF
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAIFGGLDFNNYYIGLSSAVNATSLAEAKKQGAVFAYGSFITAVLNFLILAFIIFLMVKA
HHHHCCCCCCCEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VNNLRRRLEREKPAAPAAPPPADVALLTEIRDLLAKR
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]