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The map label for this gene is nuoE [H]

Identifier: 13474500

GI number: 13474500

Start: 4313751

End: 4314230

Strand: Reverse

Name: nuoE [H]

Synonym: mll5398

Alternate gene names: 13474500

Gene position: 4314230-4313751 (Counterclockwise)

Preceding gene: 13474501

Following gene: 13474499

Centisome position: 61.32

GC content: 63.54

Gene sequence:

>480_bases
ATGACGATGCAGCCTGCAAGTACCGAGATCGCATCGCGCACGGCGGCGATTATCCAAGAACTGAAAGGCCTCGAAGGCCC
GTTGCTGCCGATCCTGCACGAGATCCAGGACGAATTCGGCCATGTGCCGCAGGCGGCGCTACCGGTCATCGCCGATGGGC
TGAACCTCTCCAGGGCCGAGGTGCACGGCGTCGTCACCTTCTACCATGATTTCCGTGCCCGGCCGGCCGGGCGGCATGTC
CTCAAGCTGTGCCAGGCGGAAGCCTGCCAGTCTATGGGCTCGGATGCCGTCGCCGCCAAGATCAAACAGTTGCTGGGCAT
CGGTTTCCACGAGACCACCCGCGACGGATCGGTTACACTCGAGCCGGTCTATTGCCTCGGGCTTTGCGCCTGTTCGCCGT
CGGCGATGCTCGACGGCGAGGTGATCGGGCGGCTCGATGACGACAAGATCGACGAGATCCTTGCCGAGGTGCGCTCATGA

Upstream 100 bases:

>100_bases
ATTTCTATCAAGCGACGGATCAGCTTTATTGATTTCGCTGGTTTCCTCTGTTTTTGTAACGACTTAGCGCTAGAACGCTA
CCGACCAGGGAGGGCGCTGC

Downstream 100 bases:

>100_bases
TCCCCCGTATCTACATTCCCGGCGATTCCGGCGCGTTGGCGCTTGGCGCCGAAAAGGTCGCCAAGGCGATCGCCAAGGAA
CTCGCGGAGCGTGGCATCGA

Product: formate dehydrogenase subunit gamma

Products: NA

Alternate protein names: NADH dehydrogenase I subunit E; NDH-1 subunit E [H]

Number of amino acids: Translated: 159; Mature: 158

Protein sequence:

>159_residues
MTMQPASTEIASRTAAIIQELKGLEGPLLPILHEIQDEFGHVPQAALPVIADGLNLSRAEVHGVVTFYHDFRARPAGRHV
LKLCQAEACQSMGSDAVAAKIKQLLGIGFHETTRDGSVTLEPVYCLGLCACSPSAMLDGEVIGRLDDDKIDEILAEVRS

Sequences:

>Translated_159_residues
MTMQPASTEIASRTAAIIQELKGLEGPLLPILHEIQDEFGHVPQAALPVIADGLNLSRAEVHGVVTFYHDFRARPAGRHV
LKLCQAEACQSMGSDAVAAKIKQLLGIGFHETTRDGSVTLEPVYCLGLCACSPSAMLDGEVIGRLDDDKIDEILAEVRS
>Mature_158_residues
TMQPASTEIASRTAAIIQELKGLEGPLLPILHEIQDEFGHVPQAALPVIADGLNLSRAEVHGVVTFYHDFRARPAGRHVL
KLCQAEACQSMGSDAVAAKIKQLLGIGFHETTRDGSVTLEPVYCLGLCACSPSAMLDGEVIGRLDDDKIDEILAEVRS

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1905

COG function: function code C; NADH:ubiquinone oxidoreductase 24 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 24 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI222080062, Length=132, Percent_Identity=31.0606060606061, Blast_Score=77, Evalue=7e-15,
Organism=Escherichia coli, GI1788621, Length=151, Percent_Identity=33.7748344370861, Blast_Score=87, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17561328, Length=134, Percent_Identity=32.089552238806, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI18859877, Length=134, Percent_Identity=30.5970149253731, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21355353, Length=136, Percent_Identity=27.9411764705882, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002023
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01257 Complex1_24kDa [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 17060; Mature: 16929

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMQPASTEIASRTAAIIQELKGLEGPLLPILHEIQDEFGHVPQAALPVIADGLNLSRAE
CCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHH
VHGVVTFYHDFRARPAGRHVLKLCQAEACQSMGSDAVAAKIKQLLGIGFHETTRDGSVTL
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCEEE
EPVYCLGLCACSPSAMLDGEVIGRLDDDKIDEILAEVRS
CHHHHHHHHHCCCCHHCCHHHHCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TMQPASTEIASRTAAIIQELKGLEGPLLPILHEIQDEFGHVPQAALPVIADGLNLSRAE
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHH
VHGVVTFYHDFRARPAGRHVLKLCQAEACQSMGSDAVAAKIKQLLGIGFHETTRDGSVTL
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCEEE
EPVYCLGLCACSPSAMLDGEVIGRLDDDKIDEILAEVRS
CHHHHHHHHHCCCCHHCCHHHHCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]