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The map label for this gene is bkdB [H]

Identifier: 13473767

GI number: 13473767

Start: 3555429

End: 3556745

Strand: Reverse

Name: bkdB [H]

Synonym: mll4471

Alternate gene names: 13473767

Gene position: 3556745-3555429 (Counterclockwise)

Preceding gene: 13473768

Following gene: 13473766

Centisome position: 50.55

GC content: 66.97

Gene sequence:

>1317_bases
ATGGGCGAACACATCATCAAGCTTCCCGATGTCGGCGAAGGCGTCGCTGAGGCCGAACTCGTCGAATGGCACGTCAAGGT
CGGCGACATGGTGCGCGAGGACACGGTGCTCGCCGCCGTCATGACCGACAAGGCGACGGTCGAGATCCCGTCGCCCGTCG
ATGGCGAGATCCTGTGGCTGGGCGCCGAGATCGGCGACACGGTGGCGATCGGCTCCCCTATCGTGCGGCTGAAAGTGGCG
GGAGAGGGCAATGTAAAGCCGAAAGGCGATGCCAAGGCCGAGGCGGTGGCCGCCGAACCGCCGGCCAAGCTCCCGACGCC
GAAGCCCGAAACCGCCGGGCCGGTCGCGAAGGCCTCGCCAAAGGCCGGCGCCCCGGAGGCGAAACCCGCTCCGGCCGTTG
CGAAAAGCACTGGGCAGAGGTCCATCTCCGGCGCGCCGCGCCCGGAGGGTGAAAGGCCGTTGGCGTCGCCGGCCGTGCGG
CTGCGGGCGAAGGAGGCCGGCATCGATCTCCGGCAGGTCGCGGGAAGCGGCCCGGCCGGGCGCATCGGCCATGAGGATAT
CGAGGCGTTCCTGGCGCGCGGCCCGCAGGTCGCCAAGACGTCCGGCCTAACCCGCAACGATGCGGTCGAGGACATCAAGG
TGGTGGGTTTGCGGCGCAAGATCGCCGAGAAGATGACGCTGTCCAAATCGCGCATCCCGCACATCACCTATGTCGAGGAG
ATCGACGTCACGGCACTCGAGGAGTTGCGCGCCGCGCTCAACAAGGAGAAGCGGGCAGACAGGCCGAAGCTGACATTGTT
GCCGTTCCTGATGCGGGCGATGGTCAAGGCGATCGCGGAGCAGCCCCAGCTCAATTCGCTGTTCGACGACGAGGCCGGCA
TCATCCACCAGCATGGCGGCATCCATATCGGCATCGCCGCGCAGACGCCGTCCGGGCTTGTGGTGCCTGTCGTCAAGCAT
GCCGAGGCGCGCGACATCTGGGATTGCGGCGCCGAGGTCAACAGGCTGGCCGAGGCGGCCAAGTCCGGCACGGCGACGCG
CGACGAGCTGTCCGGCTCGACCATCACCATCACCTCGCTCGGCGCCATGGGCGGCATTGCGACGACGCCGGTCATCAACC
ATCCGGAAGTGGCGATCATCGGCGTCAACAAGATGATGGTGCGGCCGGTGTGGGACGGCACCCAGTTCATCCCGCGCAAG
ATGATGAACCTGTCGTCCAGCTTCGACCACCGGGTGATCGACGGCTGGGATGCCGCGGTGTTCGTGCAACGGATCAAGGC
GCTGCTGGAAACGCCGGCGCTGATCTTTGTGGATTGA

Upstream 100 bases:

>100_bases
CCCTATCCGCACGCGCAGGAATGGGATTATTTCCCCGGTCCCGCCCGGGTCGGCCGCGCGCTCATCGAAACATTGGAAGC
CTAAAGTGGGGAGGCCTGAC

Downstream 100 bases:

>100_bases
GGGTGATGGCTTTGGCTCGGTCACCGCTGACAAGTTCCTGGCTGCCGATCGGTGTCGCCTGTGATTGGCTCCAGCCCTTC
ACGCGTTCGTCATCCTCGGG

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase complex component E2; BCKAD-E2; BCKADE2; Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; Dihydrolipoamide branched chain transacylase; Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase [H]

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MGEHIIKLPDVGEGVAEAELVEWHVKVGDMVREDTVLAAVMTDKATVEIPSPVDGEILWLGAEIGDTVAIGSPIVRLKVA
GEGNVKPKGDAKAEAVAAEPPAKLPTPKPETAGPVAKASPKAGAPEAKPAPAVAKSTGQRSISGAPRPEGERPLASPAVR
LRAKEAGIDLRQVAGSGPAGRIGHEDIEAFLARGPQVAKTSGLTRNDAVEDIKVVGLRRKIAEKMTLSKSRIPHITYVEE
IDVTALEELRAALNKEKRADRPKLTLLPFLMRAMVKAIAEQPQLNSLFDDEAGIIHQHGGIHIGIAAQTPSGLVVPVVKH
AEARDIWDCGAEVNRLAEAAKSGTATRDELSGSTITITSLGAMGGIATTPVINHPEVAIIGVNKMMVRPVWDGTQFIPRK
MMNLSSSFDHRVIDGWDAAVFVQRIKALLETPALIFVD

Sequences:

>Translated_438_residues
MGEHIIKLPDVGEGVAEAELVEWHVKVGDMVREDTVLAAVMTDKATVEIPSPVDGEILWLGAEIGDTVAIGSPIVRLKVA
GEGNVKPKGDAKAEAVAAEPPAKLPTPKPETAGPVAKASPKAGAPEAKPAPAVAKSTGQRSISGAPRPEGERPLASPAVR
LRAKEAGIDLRQVAGSGPAGRIGHEDIEAFLARGPQVAKTSGLTRNDAVEDIKVVGLRRKIAEKMTLSKSRIPHITYVEE
IDVTALEELRAALNKEKRADRPKLTLLPFLMRAMVKAIAEQPQLNSLFDDEAGIIHQHGGIHIGIAAQTPSGLVVPVVKH
AEARDIWDCGAEVNRLAEAAKSGTATRDELSGSTITITSLGAMGGIATTPVINHPEVAIIGVNKMMVRPVWDGTQFIPRK
MMNLSSSFDHRVIDGWDAAVFVQRIKALLETPALIFVD
>Mature_437_residues
GEHIIKLPDVGEGVAEAELVEWHVKVGDMVREDTVLAAVMTDKATVEIPSPVDGEILWLGAEIGDTVAIGSPIVRLKVAG
EGNVKPKGDAKAEAVAAEPPAKLPTPKPETAGPVAKASPKAGAPEAKPAPAVAKSTGQRSISGAPRPEGERPLASPAVRL
RAKEAGIDLRQVAGSGPAGRIGHEDIEAFLARGPQVAKTSGLTRNDAVEDIKVVGLRRKIAEKMTLSKSRIPHITYVEEI
DVTALEELRAALNKEKRADRPKLTLLPFLMRAMVKAIAEQPQLNSLFDDEAGIIHQHGGIHIGIAAQTPSGLVVPVVKHA
EARDIWDCGAEVNRLAEAAKSGTATRDELSGSTITITSLGAMGGIATTPVINHPEVAIIGVNKMMVRPVWDGTQFIPRKM
MNLSSSFDHRVIDGWDAAVFVQRIKALLETPALIFVD

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=460, Percent_Identity=30.8695652173913, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI31711992, Length=450, Percent_Identity=29.7777777777778, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI19923748, Length=230, Percent_Identity=33.9130434782609, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI203098816, Length=229, Percent_Identity=30.5676855895196, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI203098753, Length=229, Percent_Identity=30.5676855895196, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI260898739, Length=162, Percent_Identity=31.4814814814815, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1786305, Length=294, Percent_Identity=34.0136054421769, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1786946, Length=443, Percent_Identity=29.3453724604966, Blast_Score=169, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI17537937, Length=446, Percent_Identity=33.1838565022422, Blast_Score=210, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI17560088, Length=441, Percent_Identity=29.9319727891156, Blast_Score=159, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI25146366, Length=432, Percent_Identity=28.0092592592593, Blast_Score=151, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI17538894, Length=333, Percent_Identity=24.9249249249249, Blast_Score=88, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6320352, Length=436, Percent_Identity=28.4403669724771, Blast_Score=172, Evalue=8e-44,
Organism=Saccharomyces cerevisiae, GI6324258, Length=457, Percent_Identity=26.2582056892779, Blast_Score=144, Evalue=3e-35,
Organism=Drosophila melanogaster, GI18859875, Length=452, Percent_Identity=31.1946902654867, Blast_Score=176, Evalue=4e-44,
Organism=Drosophila melanogaster, GI24645909, Length=227, Percent_Identity=29.5154185022026, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24582497, Length=427, Percent_Identity=27.1662763466042, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI20129315, Length=227, Percent_Identity=28.1938325991189, Blast_Score=105, Evalue=7e-23,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.168 [H]

Molecular weight: Translated: 46401; Mature: 46270

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEHIIKLPDVGEGVAEAELVEWHVKVGDMVREDTVLAAVMTDKATVEIPSPVDGEILWL
CCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEECCEEEECCCCCCCCEEEE
GAEIGDTVAIGSPIVRLKVAGEGNVKPKGDAKAEAVAAEPPAKLPTPKPETAGPVAKASP
ECCCCCEEEECCCEEEEEECCCCCCCCCCCCCHHEEECCCCCCCCCCCCCCCCCCCCCCC
KAGAPEAKPAPAVAKSTGQRSISGAPRPEGERPLASPAVRLRAKEAGIDLRQVAGSGPAG
CCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCHHHHEEHHHHCCHHHHHCCCCCCC
RIGHEDIEAFLARGPQVAKTSGLTRNDAVEDIKVVGLRRKIAEKMTLSKSRIPHITYVEE
CCCHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHH
IDVTALEELRAALNKEKRADRPKLTLLPFLMRAMVKAIAEQPQLNSLFDDEAGIIHQHGG
CCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHCCCCCCEEEECCC
IHIGIAAQTPSGLVVPVVKHAEARDIWDCGAEVNRLAEAAKSGTATRDELSGSTITITSL
EEEEEEECCCCCCEEEEECCCCHHHHHHCCHHHHHHHHHHHCCCCCHHHCCCCEEEEEEC
GAMGGIATTPVINHPEVAIIGVNKMMVRPVWDGTQFIPRKMMNLSSSFDHRVIDGWDAAV
CCCCCCCCCCCCCCCCEEEEECCCEEEEECCCCHHHHHHHHHHHCCCCCCCEECCCHHHH
FVQRIKALLETPALIFVD
HHHHHHHHHCCCCEEEEC
>Mature Secondary Structure 
GEHIIKLPDVGEGVAEAELVEWHVKVGDMVREDTVLAAVMTDKATVEIPSPVDGEILWL
CCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEECCEEEECCCCCCCCEEEE
GAEIGDTVAIGSPIVRLKVAGEGNVKPKGDAKAEAVAAEPPAKLPTPKPETAGPVAKASP
ECCCCCEEEECCCEEEEEECCCCCCCCCCCCCHHEEECCCCCCCCCCCCCCCCCCCCCCC
KAGAPEAKPAPAVAKSTGQRSISGAPRPEGERPLASPAVRLRAKEAGIDLRQVAGSGPAG
CCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCHHHHEEHHHHCCHHHHHCCCCCCC
RIGHEDIEAFLARGPQVAKTSGLTRNDAVEDIKVVGLRRKIAEKMTLSKSRIPHITYVEE
CCCHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHH
IDVTALEELRAALNKEKRADRPKLTLLPFLMRAMVKAIAEQPQLNSLFDDEAGIIHQHGG
CCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHCCCCCCEEEECCC
IHIGIAAQTPSGLVVPVVKHAEARDIWDCGAEVNRLAEAAKSGTATRDELSGSTITITSL
EEEEEEECCCCCCEEEEECCCCHHHHHHCCHHHHHHHHHHHCCCCCHHHCCCCEEEEEEC
GAMGGIATTPVINHPEVAIIGVNKMMVRPVWDGTQFIPRKMMNLSSSFDHRVIDGWDAAV
CCCCCCCCCCCCCCCCEEEEECCCEEEEECCCCHHHHHHHHHHHCCCCCCCEECCCHHHH
FVQRIKALLETPALIFVD
HHHHHHHHHCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3046941 [H]