Click here to switch to the map view.

The map label for this gene is lpdV [H]

Identifier: 13473766

GI number: 13473766

Start: 3553715

End: 3555109

Strand: Reverse

Name: lpdV [H]

Synonym: mll4470

Alternate gene names: 13473766

Gene position: 3555109-3553715 (Counterclockwise)

Preceding gene: 13473767

Following gene: 13473764

Centisome position: 50.53

GC content: 66.38

Gene sequence:

>1395_bases
ATGAAAGAGATCTCCTGCAAGCTGCTCGTCATCGGCGCCGGCCCGGGCGGTTACATCTGCGCCATCCGTGCCGGCCAGCT
CGGCGTCGACACGGTCATCGTCGAGGCCGGTAAGCCCGGCGGCACCTGCCTCAATGTCGGCTGCATCCCGTCCAAGGCGC
TGATCCATGCGGCGGAAGAGTTCGAGAAGGTCGCCCACATGGCCGGCGGCAAGAGCCCGCTCGGCATCTCGGTATCGGCA
CCGGTGCTCGATCTTGGCAAAACCATCGCCTGGAAGGACGGCATTGTCAGCCGGCTCAACAGCGGCGTCGCCGGGCTGCT
GAAGAAGGCCGGGGTGAAGACCGTGCATGGCTGGGCGATGTTCCGCGACGGCAAGACCGTCGAAGTCGAGACCGAGACGG
GAAGCCAGGTGATCCGGGCCGAGACGATCGTCATCGCCACCGGTTCGGCGCCGGTCGAACTGCCGTTTCTGCCCTTTGGC
GGGCCTGTGATATCGTCGACGGAAGCGCTGGCCTTGAGCGTGGTGCCGAAGAAGCTTGCGGTTGTGGGCGGCGGCTATAT
CGGGCTGGAGCTTGGCATGGCCTTCGCCAAGATGGGCGCTGAAGTGACCTTGGTCGAGGCCTTGCCGCGCGTGCTGGCAC
AGTATGACGCCGAGCTGACCCGGCCCGTGGTCAAGCGGCTTGCGGCGCTTGGCGTCGAAGTCATGACCGAGGCCAAGGCC
AAGGGGCTGTCGACCAAGGGCGACGCGCTGCTGGTCGAAACAGCGGACGGCAAGAATACCAAGGTTTCCGCCGACAAGAT
CCTGGTGACGGTCGGCCGCAAGCCGGTGACCGAAGGCTGGGGCCTCGACCAGATCGACCTCGACATGTCAGGCAAATTCA
TCCGCATCGACGACCAGTGCCGCACCTCGATGCGCGGCATCTTCGCCATCGGCGACGTCACCGGCGAGCCGATGCTGGCG
CATCGGGCGATGGCGCAAGGCGAAATGGTCGCGGAGATCGTCGCCGGCCACAAGCGCAACTGGGACAAGCGCGCAATCCC
CGCCGTCTGCTTCACTGATCCGGAGCTGGTGACGGTTGGGCTGTCGCCCGAGGAAGCCAAGGCCCAGGGCGAGATCAAGA
TCGGGCTGTTCCCGTTCGCCGCCAACGGACGGGCGATGACGAAGCTCGGCGAGGATGGTTTCGTCCGCGTCGTGGCGCGG
GCCGACAACCATCTGGTGCTCGGCATCCAGGCGGTCGGGCAGGGCGTGTCGGAACTGTCGACGGCGTTCGGCCTGGCGCT
GGAGATGGGGGCGCGGCTGGAGGACATCGCCGGCACCATCCATGCGCACCCGACGCAAGGCGAGGGGTTCCAGGAAGCGG
CGCTGAAGGCGCTCGGGCACGCGCTGCATATTTAG

Upstream 100 bases:

>100_bases
TCCTTGCTCCGCCCCAGGATGACGAAGGCGCGAGCGCCTCGGCCAATCCTAGACGTAAGCGATACGGCACGAAGGCAAGG
CGACCGATGGAGGTGACCCC

Downstream 100 bases:

>100_bases
AGGTTTTACCCTCCCCCTTGTGGGGAGGGTCGATCCGGGAAGCGGAGCGGGGTGGGGGGTCTTCTCCGATCGTCGGCGCT
CACCCCCACCCCGTCCCGCA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of branched-chain alpha-keto acid dehydrogenase complex; LPD-Val [H]

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MKEISCKLLVIGAGPGGYICAIRAGQLGVDTVIVEAGKPGGTCLNVGCIPSKALIHAAEEFEKVAHMAGGKSPLGISVSA
PVLDLGKTIAWKDGIVSRLNSGVAGLLKKAGVKTVHGWAMFRDGKTVEVETETGSQVIRAETIVIATGSAPVELPFLPFG
GPVISSTEALALSVVPKKLAVVGGGYIGLELGMAFAKMGAEVTLVEALPRVLAQYDAELTRPVVKRLAALGVEVMTEAKA
KGLSTKGDALLVETADGKNTKVSADKILVTVGRKPVTEGWGLDQIDLDMSGKFIRIDDQCRTSMRGIFAIGDVTGEPMLA
HRAMAQGEMVAEIVAGHKRNWDKRAIPAVCFTDPELVTVGLSPEEAKAQGEIKIGLFPFAANGRAMTKLGEDGFVRVVAR
ADNHLVLGIQAVGQGVSELSTAFGLALEMGARLEDIAGTIHAHPTQGEGFQEAALKALGHALHI

Sequences:

>Translated_464_residues
MKEISCKLLVIGAGPGGYICAIRAGQLGVDTVIVEAGKPGGTCLNVGCIPSKALIHAAEEFEKVAHMAGGKSPLGISVSA
PVLDLGKTIAWKDGIVSRLNSGVAGLLKKAGVKTVHGWAMFRDGKTVEVETETGSQVIRAETIVIATGSAPVELPFLPFG
GPVISSTEALALSVVPKKLAVVGGGYIGLELGMAFAKMGAEVTLVEALPRVLAQYDAELTRPVVKRLAALGVEVMTEAKA
KGLSTKGDALLVETADGKNTKVSADKILVTVGRKPVTEGWGLDQIDLDMSGKFIRIDDQCRTSMRGIFAIGDVTGEPMLA
HRAMAQGEMVAEIVAGHKRNWDKRAIPAVCFTDPELVTVGLSPEEAKAQGEIKIGLFPFAANGRAMTKLGEDGFVRVVAR
ADNHLVLGIQAVGQGVSELSTAFGLALEMGARLEDIAGTIHAHPTQGEGFQEAALKALGHALHI
>Mature_464_residues
MKEISCKLLVIGAGPGGYICAIRAGQLGVDTVIVEAGKPGGTCLNVGCIPSKALIHAAEEFEKVAHMAGGKSPLGISVSA
PVLDLGKTIAWKDGIVSRLNSGVAGLLKKAGVKTVHGWAMFRDGKTVEVETETGSQVIRAETIVIATGSAPVELPFLPFG
GPVISSTEALALSVVPKKLAVVGGGYIGLELGMAFAKMGAEVTLVEALPRVLAQYDAELTRPVVKRLAALGVEVMTEAKA
KGLSTKGDALLVETADGKNTKVSADKILVTVGRKPVTEGWGLDQIDLDMSGKFIRIDDQCRTSMRGIFAIGDVTGEPMLA
HRAMAQGEMVAEIVAGHKRNWDKRAIPAVCFTDPELVTVGLSPEEAKAQGEIKIGLFPFAANGRAMTKLGEDGFVRVVAR
ADNHLVLGIQAVGQGVSELSTAFGLALEMGARLEDIAGTIHAHPTQGEGFQEAALKALGHALHI

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=467, Percent_Identity=40.0428265524625, Blast_Score=291, Evalue=1e-78,
Organism=Homo sapiens, GI50301238, Length=464, Percent_Identity=29.5258620689655, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI22035672, Length=459, Percent_Identity=29.6296296296296, Blast_Score=129, Evalue=8e-30,
Organism=Homo sapiens, GI291045266, Length=463, Percent_Identity=26.133909287257, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI148277071, Length=437, Percent_Identity=24.9427917620137, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI148277065, Length=437, Percent_Identity=24.9427917620137, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI33519430, Length=437, Percent_Identity=24.9427917620137, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI33519428, Length=437, Percent_Identity=24.9427917620137, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI33519426, Length=437, Percent_Identity=24.9427917620137, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI291045268, Length=453, Percent_Identity=24.2825607064018, Blast_Score=93, Evalue=6e-19,
Organism=Escherichia coli, GI1786307, Length=462, Percent_Identity=41.1255411255411, Blast_Score=323, Evalue=1e-89,
Organism=Escherichia coli, GI1789915, Length=437, Percent_Identity=31.5789473684211, Blast_Score=195, Evalue=6e-51,
Organism=Escherichia coli, GI87082354, Length=463, Percent_Identity=26.7818574514039, Blast_Score=141, Evalue=9e-35,
Organism=Escherichia coli, GI87081717, Length=454, Percent_Identity=25.5506607929515, Blast_Score=129, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI32565766, Length=461, Percent_Identity=40.997830802603, Blast_Score=300, Evalue=1e-81,
Organism=Caenorhabditis elegans, GI17557007, Length=476, Percent_Identity=28.1512605042017, Blast_Score=141, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI71983429, Length=439, Percent_Identity=29.3849658314351, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI71983419, Length=439, Percent_Identity=29.3849658314351, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI71982272, Length=494, Percent_Identity=26.7206477732794, Blast_Score=99, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6321091, Length=477, Percent_Identity=36.4779874213836, Blast_Score=265, Evalue=8e-72,
Organism=Saccharomyces cerevisiae, GI6325166, Length=463, Percent_Identity=31.317494600432, Blast_Score=174, Evalue=4e-44,
Organism=Saccharomyces cerevisiae, GI6325240, Length=478, Percent_Identity=26.7782426778243, Blast_Score=151, Evalue=2e-37,
Organism=Drosophila melanogaster, GI21358499, Length=462, Percent_Identity=39.8268398268398, Blast_Score=282, Evalue=4e-76,
Organism=Drosophila melanogaster, GI24640549, Length=475, Percent_Identity=28, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24640553, Length=475, Percent_Identity=28, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24640551, Length=475, Percent_Identity=28, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI17737741, Length=483, Percent_Identity=26.7080745341615, Blast_Score=125, Evalue=7e-29,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 48312; Mature: 48312

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEISCKLLVIGAGPGGYICAIRAGQLGVDTVIVEAGKPGGTCLNVGCIPSKALIHAAEE
CCCCEEEEEEEECCCCCEEEEEECCCCCCEEEEEECCCCCCCEEEECCCCCHHHHHHHHH
FEKVAHMAGGKSPLGISVSAPVLDLGKTIAWKDGIVSRLNSGVAGLLKKAGVKTVHGWAM
HHHHHHHCCCCCCCCEEECCCHHHCCCEEEEHHHHHHHHCCCHHHHHHHCCCCEEEEEEE
FRDGKTVEVETETGSQVIRAETIVIATGSAPVELPFLPFGGPVISSTEALALSVVPKKLA
EECCCEEEEECCCCCEEEEEEEEEEEECCCCEEEEEECCCCCCCCCCCEEEEEECCCEEE
VVGGGYIGLELGMAFAKMGAEVTLVEALPRVLAQYDAELTRPVVKRLAALGVEVMTEAKA
EECCCEEHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
KGLSTKGDALLVETADGKNTKVSADKILVTVGRKPVTEGWGLDQIDLDMSGKFIRIDDQC
CCCCCCCCEEEEEECCCCCCEEECCEEEEEECCCCCCCCCCCCEEEECCCCCEEEECHHH
RTSMRGIFAIGDVTGEPMLAHRAMAQGEMVAEIVAGHKRNWDKRAIPAVCFTDPELVTVG
HHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCEEEEE
LSPEEAKAQGEIKIGLFPFAANGRAMTKLGEDGFVRVVARADNHLVLGIQAVGQGVSELS
CCCHHHCCCCCEEEEEEEEECCCCEEEECCCCCEEEEEEECCCEEEEEHHHHCCCHHHHH
TAFGLALEMGARLEDIAGTIHAHPTQGEGFQEAALKALGHALHI
HHHHHHHHHCCCHHHHCCCEECCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKEISCKLLVIGAGPGGYICAIRAGQLGVDTVIVEAGKPGGTCLNVGCIPSKALIHAAEE
CCCCEEEEEEEECCCCCEEEEEECCCCCCEEEEEECCCCCCCEEEECCCCCHHHHHHHHH
FEKVAHMAGGKSPLGISVSAPVLDLGKTIAWKDGIVSRLNSGVAGLLKKAGVKTVHGWAM
HHHHHHHCCCCCCCCEEECCCHHHCCCEEEEHHHHHHHHCCCHHHHHHHCCCCEEEEEEE
FRDGKTVEVETETGSQVIRAETIVIATGSAPVELPFLPFGGPVISSTEALALSVVPKKLA
EECCCEEEEECCCCCEEEEEEEEEEEECCCCEEEEEECCCCCCCCCCCEEEEEECCCEEE
VVGGGYIGLELGMAFAKMGAEVTLVEALPRVLAQYDAELTRPVVKRLAALGVEVMTEAKA
EECCCEEHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
KGLSTKGDALLVETADGKNTKVSADKILVTVGRKPVTEGWGLDQIDLDMSGKFIRIDDQC
CCCCCCCCEEEEEECCCCCCEEECCEEEEEECCCCCCCCCCCCEEEECCCCCEEEECHHH
RTSMRGIFAIGDVTGEPMLAHRAMAQGEMVAEIVAGHKRNWDKRAIPAVCFTDPELVTVG
HHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCEEEEE
LSPEEAKAQGEIKIGLFPFAANGRAMTKLGEDGFVRVVARADNHLVLGIQAVGQGVSELS
CCCHHHCCCCCEEEEEEEEECCCCEEEECCCCCEEEEEEECCCEEEEEHHHHCCCHHHHH
TAFGLALEMGARLEDIAGTIHAHPTQGEGFQEAALKALGHALHI
HHHHHHHHHCCCHHHHCCCEECCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]