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The map label for this gene is puuB [H]

Identifier: 13473543

GI number: 13473543

Start: 3338223

End: 3339521

Strand: Reverse

Name: puuB [H]

Synonym: mll4186

Alternate gene names: 13473543

Gene position: 3339521-3338223 (Counterclockwise)

Preceding gene: 13473544

Following gene: 13473541

Centisome position: 47.46

GC content: 65.43

Gene sequence:

>1299_bases
ATGGCATCTTCAACGGGCTTCAATTCCGGTCTCGATATCGGCAAATCCTACTATATCGCCACCGCCAATCCGGCGCCGGA
CCATCCGGCGCTTGTCGGCGATGTCGTAGCCGATCTGGTCGTCGTCGGCGGCGGCTGCACCGGCCTGTCGGCGGCCCTTC
ACGCCGCCGAACGCGGCCTGAAGGTGGTGCTGCTCGAAGGAGGCAAGATCGGCTGGGGCGCATCGGGGCGCAATGGCGGC
CAGATGATCCCCGGCCTGCGCAAGGGCGCCAAGGGCCTGGTCAAGCTGTACGGGCCGGAGCGGGCGAAGGTGCTGTTCGA
CCTCGCCTTCGAGGCGCGCGGCCTGGTGCTTGATATCATCGAGCGCCATGCCATCGATTGCGACCTGAGACTGACCGGCC
ATTTGGTCGGCGCAGTCAACGGCTCCGATCTCAAGGATTTGGAGGAAGAGGCCAAGTGCCTCGAAAGCGTGATGAAGTTC
CGCGATGTCGAGATCCTCTCGGCGGCGGAAGCACGCGCCAAGGTCGACACGCCCTATCACGGCGCGATGTACGAGCCGCT
CGGCGGCCACATGCATCCGCTGAACTACACGCTTGGCCTTGCCCGCGCGGCCGTGGCCGCCGGCGTCGTTATCCACGAGA
ATTCAGTGGCGGTGAAGCTGGAGCGCGAGCCTTCGATCCGCGTCTCGACATCGAAGGGTTCGGTGCGCGCAAAACATGTC
GTGCTGGCGGGCGACGCGCTGCTCCATGGATTGGAGCCGCGCGTCAACAGCCGCATCATGCCGGTCGGCAATTACATCGT
CGCCACCGAGCCGCTGGAGGGCAAACGCAATGTCATCCCGGCCAATGTCGCGGTCTCCGACACGCGCTTCGTCGTCAATT
ACTATCGCATGTCGGCGGACGGACGTCTGCTGTTCGGCGGCGGCGAGCGCTACACGCCGTCACCGCCGGCCGACATTGCC
GGCTTCGTGCGGCCGCATATGGAAGCTACCTTCCCGCAGCTCAAGGGCTGCCGCATCGATCATGCCTGGGGCGGGCTGGT
GTCGGTGACGACATCGCGGCTGCCGCATGTCGGGCACTATGGCGAGGTCTATTTCGCGCATGGCTATTCCGGCAAGGGCG
TCATCCTGTCGACGCTGTCGGGCAGGCTGCTCGCCGAAGCGATCACGGGGGATGCCTCGCGACTCGACCTGTTCTCGACG
CTGACCCCGCTGCCGTTCCCCGGTGGCACGGCGCTGCGCGGCCCGCTCTATGTGCTGGGCATGCTGTGGTATGCGATGCG
GGACCGCATCAAGCATTGA

Upstream 100 bases:

>100_bases
TCTCCCAGACCTATCTGCGCGTGAAGAGCGTCGAGCTCGACCTGTTCCAGAGCGTGGTGACGAGCTGGGAACGCGATCAC
CTGCTGCTGAAGGTGTGATC

Downstream 100 bases:

>100_bases
GGTCGCGAGAAGCGACCTAAACGACGAAGAAATCCTCGATGCGCTGCCTGGCCTCAAGCAGCGCCGGCAGGATTTCCCGC
TCCATCTCCGCGACCGAAAA

Product: oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MASSTGFNSGLDIGKSYYIATANPAPDHPALVGDVVADLVVVGGGCTGLSAALHAAERGLKVVLLEGGKIGWGASGRNGG
QMIPGLRKGAKGLVKLYGPERAKVLFDLAFEARGLVLDIIERHAIDCDLRLTGHLVGAVNGSDLKDLEEEAKCLESVMKF
RDVEILSAAEARAKVDTPYHGAMYEPLGGHMHPLNYTLGLARAAVAAGVVIHENSVAVKLEREPSIRVSTSKGSVRAKHV
VLAGDALLHGLEPRVNSRIMPVGNYIVATEPLEGKRNVIPANVAVSDTRFVVNYYRMSADGRLLFGGGERYTPSPPADIA
GFVRPHMEATFPQLKGCRIDHAWGGLVSVTTSRLPHVGHYGEVYFAHGYSGKGVILSTLSGRLLAEAITGDASRLDLFST
LTPLPFPGGTALRGPLYVLGMLWYAMRDRIKH

Sequences:

>Translated_432_residues
MASSTGFNSGLDIGKSYYIATANPAPDHPALVGDVVADLVVVGGGCTGLSAALHAAERGLKVVLLEGGKIGWGASGRNGG
QMIPGLRKGAKGLVKLYGPERAKVLFDLAFEARGLVLDIIERHAIDCDLRLTGHLVGAVNGSDLKDLEEEAKCLESVMKF
RDVEILSAAEARAKVDTPYHGAMYEPLGGHMHPLNYTLGLARAAVAAGVVIHENSVAVKLEREPSIRVSTSKGSVRAKHV
VLAGDALLHGLEPRVNSRIMPVGNYIVATEPLEGKRNVIPANVAVSDTRFVVNYYRMSADGRLLFGGGERYTPSPPADIA
GFVRPHMEATFPQLKGCRIDHAWGGLVSVTTSRLPHVGHYGEVYFAHGYSGKGVILSTLSGRLLAEAITGDASRLDLFST
LTPLPFPGGTALRGPLYVLGMLWYAMRDRIKH
>Mature_431_residues
ASSTGFNSGLDIGKSYYIATANPAPDHPALVGDVVADLVVVGGGCTGLSAALHAAERGLKVVLLEGGKIGWGASGRNGGQ
MIPGLRKGAKGLVKLYGPERAKVLFDLAFEARGLVLDIIERHAIDCDLRLTGHLVGAVNGSDLKDLEEEAKCLESVMKFR
DVEILSAAEARAKVDTPYHGAMYEPLGGHMHPLNYTLGLARAAVAAGVVIHENSVAVKLEREPSIRVSTSKGSVRAKHVV
LAGDALLHGLEPRVNSRIMPVGNYIVATEPLEGKRNVIPANVAVSDTRFVVNYYRMSADGRLLFGGGERYTPSPPADIAG
FVRPHMEATFPQLKGCRIDHAWGGLVSVTTSRLPHVGHYGEVYFAHGYSGKGVILSTLSGRLLAEAITGDASRLDLFSTL
TPLPFPGGTALRGPLYVLGMLWYAMRDRIKH

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=420, Percent_Identity=41.4285714285714, Blast_Score=312, Evalue=3e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 45968; Mature: 45836

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASSTGFNSGLDIGKSYYIATANPAPDHPALVGDVVADLVVVGGGCTGLSAALHAAERGL
CCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHEECCCCHHHHHHHHHHHCCC
KVVLLEGGKIGWGASGRNGGQMIPGLRKGAKGLVKLYGPERAKVLFDLAFEARGLVLDII
EEEEEECCEEECCCCCCCCCCCCCCHHHCCCCEEEECCCCHHEEEEEEHHCCCCHHHHHH
ERHAIDCDLRLTGHLVGAVNGSDLKDLEEEAKCLESVMKFRDVEILSAAEARAKVDTPYH
HHHCCCEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCC
GAMYEPLGGHMHPLNYTLGLARAAVAAGVVIHENSVAVKLEREPSIRVSTSKGSVRAKHV
CCCCCCCCCCCCCCHHHHHHHHHHHHHCEEEECCCEEEEEECCCCEEEECCCCCEEEEEE
VLAGDALLHGLEPRVNSRIMPVGNYIVATEPLEGKRNVIPANVAVSDTRFVVNYYRMSAD
EEECHHHHHCCCCCCCCEEEECCCEEEEECCCCCCCCEEECCEEECCCEEEEEEEEECCC
GRLLFGGGERYTPSPPADIAGFVRPHMEATFPQLKGCRIDHAWGGLVSVTTSRLPHVGHY
CEEEECCCCCCCCCCCHHHHHHHHHHHHHCCHHHCCCEEECCCCCEEEEHHHCCCCCCCC
GEVYFAHGYSGKGVILSTLSGRLLAEAITGDASRLDLFSTLTPLPFPGGTALRGPLYVLG
CCEEEEECCCCCCEEEEECCCHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
MLWYAMRDRIKH
HHHHHHHHHHCC
>Mature Secondary Structure 
ASSTGFNSGLDIGKSYYIATANPAPDHPALVGDVVADLVVVGGGCTGLSAALHAAERGL
CCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHEECCCCHHHHHHHHHHHCCC
KVVLLEGGKIGWGASGRNGGQMIPGLRKGAKGLVKLYGPERAKVLFDLAFEARGLVLDII
EEEEEECCEEECCCCCCCCCCCCCCHHHCCCCEEEECCCCHHEEEEEEHHCCCCHHHHHH
ERHAIDCDLRLTGHLVGAVNGSDLKDLEEEAKCLESVMKFRDVEILSAAEARAKVDTPYH
HHHCCCEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCC
GAMYEPLGGHMHPLNYTLGLARAAVAAGVVIHENSVAVKLEREPSIRVSTSKGSVRAKHV
CCCCCCCCCCCCCCHHHHHHHHHHHHHCEEEECCCEEEEEECCCCEEEECCCCCEEEEEE
VLAGDALLHGLEPRVNSRIMPVGNYIVATEPLEGKRNVIPANVAVSDTRFVVNYYRMSAD
EEECHHHHHCCCCCCCCEEEECCCEEEEECCCCCCCCEEECCEEECCCEEEEEEEEECCC
GRLLFGGGERYTPSPPADIAGFVRPHMEATFPQLKGCRIDHAWGGLVSVTTSRLPHVGHY
CEEEECCCCCCCCCCCHHHHHHHHHHHHHCCHHHCCCEEECCCCCEEEEHHHCCCCCCCC
GEVYFAHGYSGKGVILSTLSGRLLAEAITGDASRLDLFSTLTPLPFPGGTALRGPLYVLG
CCEEEEECCCCCCEEEEECCCHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
MLWYAMRDRIKH
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]