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The map label for this gene is puuA [H]

Identifier: 13473544

GI number: 13473544

Start: 3339524

End: 3340894

Strand: Reverse

Name: puuA [H]

Synonym: mll4187

Alternate gene names: 13473544

Gene position: 3340894-3339524 (Counterclockwise)

Preceding gene: 13473545

Following gene: 13473543

Centisome position: 47.48

GC content: 65.5

Gene sequence:

>1371_bases
ATGCTTGATAAGAAGACGAATATCGCGACCGACGATCCCAAGGCCTGGCTGGCACAGCACGGCATCAACGAGGTCGAATG
CCTGGTGCCCGACATGAACGGCGTGCTGCGCGGCAAGGCGCTGCCGACGGCGAAATTCCTCAAGGCGCTGGAAGACCGCG
CGCTCTATCTGCCGAGCAGCGCCTTCCTGGTCAGCATCGACGGCCGCTATTCCGGCTCGATCGACGAGGGCTTTGCCTAT
TCGGATCCGGACATGCGCATGGTGCCTGACGTTTCGACGCTCTGCCTGGCGCCGGGCGCCGGGGCGGGGAAGGCCTATGT
CTTCGCCGACGCCTTCCACATGGATGGCCGGCCATGGATGGCCTCGCCGCGGCATGTGCTGCGCGCCGTGCTCGATCTCT
ACCGCCAGCGCGGCTGGCGGGCGGTGGTGGCGCCGGAGGTCGAATTCTATCTGACCGCGCCCAACCCCGATCCGGACAGG
CCACTGACCGCGCCGGTCGGCGCCAATGGCCGCGCCGAGGGCGTGCAGCATCCCTACGACATGGTGGCACTGGAGGAATT
CGAGCCGGTGATCCGGCGCGTCTACGACTACGCCGCGGCCGCCGGGCTGCCGCTTGACACGCTGATCCATGAATCGGGCA
CGGCGCAGCTGGAGATCAATCTCCTGCATGGCGACGCGCTGCCGCTGGCCGACCAGGTGCTTTTATTCAAGCGGCTGACG
CGCCAGGCCGCGCAGCAATGCGGCATGCATGCCACCTTCATGGCCAAGCCGATCGCGGCCCAGGCGGGCAGCTCGATGCA
CCTGCACATGTCCGTTGTCGATGAGGCGGGCAATGCGCTGTTTGCCAGCGATGATGATGCCGACACCGAAATGTTCGGCC
ATTTCATTGGCGGCCTGCAGACATATGTGCCGGAGATCATGCCGCTGTTCGCGCCCAACGTGAATTCGTTCCGGCGGATA
CGGCCGAACCACAGTGCGCCGGCTAACATCGAATGGTCGCACGACAACCGCTCCTGCGGCCTGCGCGTGCCGGCGGGTGG
CCGCTCGGCGCGGCGGGTGGAGAACCGCTTGCCGGGCGCCGATTCCAATCCCTATCTGGCGATCGCCGGCTCGTTGCTCG
CCGGCTATCTCGGCGTCCAGCAGAAACTGGCGCGCTCGGCCGAGGCTTCTGGCAATGCCTACAAGATCAAGAGCACGCTG
CCGAAAACCATGGAGGAGGCGCTCGACCGCTTCACCGCCTGTGAGCCGGTGCGCAAACTGCTCGGCGAGGATTTCTCCCA
GACCTATCTGCGCGTGAAGAGCGTCGAGCTCGACCTGTTCCAGAGCGTGGTGACGAGCTGGGAACGCGATCACCTGCTGC
TGAAGGTGTGA

Upstream 100 bases:

>100_bases
TGCGCGCCCGCCTGAGGGACAATGCAGCCCAGATGGCGCTGCAACCCGGAACGGACGTGGCCGCGCAAGCCATACTCTCT
CTGATACGGACTTGAACGAC

Downstream 100 bases:

>100_bases
TCATGGCATCTTCAACGGGCTTCAATTCCGGTCTCGATATCGGCAAATCCTACTATATCGCCACCGCCAATCCGGCGCCG
GACCATCCGGCGCTTGTCGG

Product: glutamine synthetase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MLDKKTNIATDDPKAWLAQHGINEVECLVPDMNGVLRGKALPTAKFLKALEDRALYLPSSAFLVSIDGRYSGSIDEGFAY
SDPDMRMVPDVSTLCLAPGAGAGKAYVFADAFHMDGRPWMASPRHVLRAVLDLYRQRGWRAVVAPEVEFYLTAPNPDPDR
PLTAPVGANGRAEGVQHPYDMVALEEFEPVIRRVYDYAAAAGLPLDTLIHESGTAQLEINLLHGDALPLADQVLLFKRLT
RQAAQQCGMHATFMAKPIAAQAGSSMHLHMSVVDEAGNALFASDDDADTEMFGHFIGGLQTYVPEIMPLFAPNVNSFRRI
RPNHSAPANIEWSHDNRSCGLRVPAGGRSARRVENRLPGADSNPYLAIAGSLLAGYLGVQQKLARSAEASGNAYKIKSTL
PKTMEEALDRFTACEPVRKLLGEDFSQTYLRVKSVELDLFQSVVTSWERDHLLLKV

Sequences:

>Translated_456_residues
MLDKKTNIATDDPKAWLAQHGINEVECLVPDMNGVLRGKALPTAKFLKALEDRALYLPSSAFLVSIDGRYSGSIDEGFAY
SDPDMRMVPDVSTLCLAPGAGAGKAYVFADAFHMDGRPWMASPRHVLRAVLDLYRQRGWRAVVAPEVEFYLTAPNPDPDR
PLTAPVGANGRAEGVQHPYDMVALEEFEPVIRRVYDYAAAAGLPLDTLIHESGTAQLEINLLHGDALPLADQVLLFKRLT
RQAAQQCGMHATFMAKPIAAQAGSSMHLHMSVVDEAGNALFASDDDADTEMFGHFIGGLQTYVPEIMPLFAPNVNSFRRI
RPNHSAPANIEWSHDNRSCGLRVPAGGRSARRVENRLPGADSNPYLAIAGSLLAGYLGVQQKLARSAEASGNAYKIKSTL
PKTMEEALDRFTACEPVRKLLGEDFSQTYLRVKSVELDLFQSVVTSWERDHLLLKV
>Mature_456_residues
MLDKKTNIATDDPKAWLAQHGINEVECLVPDMNGVLRGKALPTAKFLKALEDRALYLPSSAFLVSIDGRYSGSIDEGFAY
SDPDMRMVPDVSTLCLAPGAGAGKAYVFADAFHMDGRPWMASPRHVLRAVLDLYRQRGWRAVVAPEVEFYLTAPNPDPDR
PLTAPVGANGRAEGVQHPYDMVALEEFEPVIRRVYDYAAAAGLPLDTLIHESGTAQLEINLLHGDALPLADQVLLFKRLT
RQAAQQCGMHATFMAKPIAAQAGSSMHLHMSVVDEAGNALFASDDDADTEMFGHFIGGLQTYVPEIMPLFAPNVNSFRRI
RPNHSAPANIEWSHDNRSCGLRVPAGGRSARRVENRLPGADSNPYLAIAGSLLAGYLGVQQKLARSAEASGNAYKIKSTL
PKTMEEALDRFTACEPVRKLLGEDFSQTYLRVKSVELDLFQSVVTSWERDHLLLKV

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=430, Percent_Identity=21.8604651162791, Blast_Score=98, Evalue=1e-20,
Organism=Escherichia coli, GI87081870, Length=457, Percent_Identity=33.6980306345733, Blast_Score=237, Evalue=9e-64,
Organism=Escherichia coli, GI1790301, Length=220, Percent_Identity=33.6363636363636, Blast_Score=112, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 49955; Mature: 49955

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDKKTNIATDDPKAWLAQHGINEVECLVPDMNGVLRGKALPTAKFLKALEDRALYLPSS
CCCCCCCCCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCEEECCCC
AFLVSIDGRYSGSIDEGFAYSDPDMRMVPDVSTLCLAPGAGAGKAYVFADAFHMDGRPWM
EEEEEECCCCCCCCCCCCCCCCCCCEECCCCHHHEECCCCCCCCEEEEEEEEECCCCCCC
ASPRHVLRAVLDLYRQRGWRAVVAPEVEFYLTAPNPDPDRPLTAPVGANGRAEGVQHPYD
CCHHHHHHHHHHHHHHCCCEEEECCCEEEEEECCCCCCCCCEECCCCCCCCCCCCCCCHH
MVALEEFEPVIRRVYDYAAAAGLPLDTLIHESGTAQLEINLLHGDALPLADQVLLFKRLT
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHH
RQAAQQCGMHATFMAKPIAAQAGSSMHLHMSVVDEAGNALFASDDDADTEMFGHFIGGLQ
HHHHHHCCCCCHHHHCCHHHHCCCCEEEEEEEEECCCCEEEECCCCCHHHHHHHHHHHHH
TYVPEIMPLFAPNVNSFRRIRPNHSAPANIEWSHDNRSCGLRVPAGGRSARRVENRLPGA
HHHHHHHHHHCCCCCHHHHCCCCCCCCCCEEECCCCCCCCEECCCCCCHHHHHHHHCCCC
DSNPYLAIAGSLLAGYLGVQQKLARSAEASGNAYKIKSTLPKTMEEALDRFTACEPVRKL
CCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHH
LGEDFSQTYLRVKSVELDLFQSVVTSWERDHLLLKV
HCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEC
>Mature Secondary Structure
MLDKKTNIATDDPKAWLAQHGINEVECLVPDMNGVLRGKALPTAKFLKALEDRALYLPSS
CCCCCCCCCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCEEECCCC
AFLVSIDGRYSGSIDEGFAYSDPDMRMVPDVSTLCLAPGAGAGKAYVFADAFHMDGRPWM
EEEEEECCCCCCCCCCCCCCCCCCCEECCCCHHHEECCCCCCCCEEEEEEEEECCCCCCC
ASPRHVLRAVLDLYRQRGWRAVVAPEVEFYLTAPNPDPDRPLTAPVGANGRAEGVQHPYD
CCHHHHHHHHHHHHHHCCCEEEECCCEEEEEECCCCCCCCCEECCCCCCCCCCCCCCCHH
MVALEEFEPVIRRVYDYAAAAGLPLDTLIHESGTAQLEINLLHGDALPLADQVLLFKRLT
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHH
RQAAQQCGMHATFMAKPIAAQAGSSMHLHMSVVDEAGNALFASDDDADTEMFGHFIGGLQ
HHHHHHCCCCCHHHHCCHHHHCCCCEEEEEEEEECCCCEEEECCCCCHHHHHHHHHHHHH
TYVPEIMPLFAPNVNSFRRIRPNHSAPANIEWSHDNRSCGLRVPAGGRSARRVENRLPGA
HHHHHHHHHHCCCCCHHHHCCCCCCCCCCEEECCCCCCCCEECCCCCCHHHHHHHHCCCC
DSNPYLAIAGSLLAGYLGVQQKLARSAEASGNAYKIKSTLPKTMEEALDRFTACEPVRKL
CCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHH
LGEDFSQTYLRVKSVELDLFQSVVTSWERDHLLLKV
HCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]