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The map label for this gene is rfaF [H]

Identifier: 13472312

GI number: 13472312

Start: 2076315

End: 2077322

Strand: Direct

Name: rfaF [H]

Synonym: mlr2566

Alternate gene names: 13472312

Gene position: 2076315-2077322 (Clockwise)

Preceding gene: 13472311

Following gene: 13472313

Centisome position: 29.51

GC content: 66.07

Gene sequence:

>1008_bases
ATGGCTGAAAGCCCAACGATCCTCGTGATCGGCCCACGCTGGGTGGGCGACATGGTGATGGCGCAGTGCCTGTTCTCGGC
GCTGAAGGAACTCTATCCCAACGCCGCCATCGACGTGCTGGCACCGGCCTGGGCGGCGCCGCTGGTCAAGCGTATGCCCG
AGATCCGCCAGCAGATCGACTTGCCGCTGAAGCCCGGCGCGCTCGAATTCACCATTCGCCGCCGCTTCGGCCGTCTGCTG
CGCGGCCGCTACGACATGGCCTATGTCCTGCCAGGTAGCTGGAAATCGGCGCTGATCCCGTTCTTTGCCCGCATTCCGCG
CCGTTTCGGCCATCTGCGCGAGATGCGCTACGGCCTTTTGACCGACATCGTGCCGTTGCCGGACGCCGTGAAACGGCGCA
CAGCGCAGGCCTATTTCAGTCTCGCCAAGGGCGGCAGTTTTCGCGCGCCCCATCTGACCGTGGATGCCGGAAACCAGGCC
GCCCTGCTGGACCGTTTCGGGCTGGCGCCGCGGAAATTCGCGGCTCTGATGCCTGGCGCCGAATTCGGTCCGGCCAAGCG
CTGGCCGAGCGAAAGCTATGCCGGCCTTGCCCGCAAGTTCATGGATAAGGGATTGAAGGTCGCCCTGTTCGGTTCGAAGA
ACGACCGCGATGTCACCGCTGAGATCACGGCCCTTGCCCCCGGCGTCGTCGACCTAGCCGGCCAGACACGGCTGGAGGAC
GCCATCGACCTGATAGCGGCGGCAAGGCTGGCGGTCTCCAACGACAGCGGGCTGATGCATGTCGCGGCGGGCGTCGGTAC
GCCGATCGTTGCCGTCTACGGCTCGACCTCGCCCGAAAACACGCCGCCGCTGGCGGAACGGCGCGAGCTGGTCTGGCTGC
ACCTCTCCTGCTCGCCCTGCCACCAGAAGGTCTGCCCGCTCGGCCATCTCAACTGCCTGAAGACATTGGAAGTCGGCCAA
GTCGCTACAGCGGCGGAGCGACTGCTGGAACTCCCGGCCACGGCATGA

Upstream 100 bases:

>100_bases
GCTACCAGAGCTTCACGCAAGCTGATATGTCCCGTTTGCGCGCGGCCGGGTATAATGGCCAGTTCCGGACAGTGGAAACC
GGTGTCAGAGACTATGTCGA

Downstream 100 bases:

>100_bases
AGGTTCTGATCGTCAAGACATCGTCCATGGGCGATGTCATCCACACCTTTCCGGCCGTCGAGGATGCCAGCCTGCACCGG
CCGGACATTTCCTTCGACTG

Product: ADP-heptose-LPS heptosyltransferase

Products: NA

Alternate protein names: ADP-heptose--LPS heptosyltransferase II [H]

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MAESPTILVIGPRWVGDMVMAQCLFSALKELYPNAAIDVLAPAWAAPLVKRMPEIRQQIDLPLKPGALEFTIRRRFGRLL
RGRYDMAYVLPGSWKSALIPFFARIPRRFGHLREMRYGLLTDIVPLPDAVKRRTAQAYFSLAKGGSFRAPHLTVDAGNQA
ALLDRFGLAPRKFAALMPGAEFGPAKRWPSESYAGLARKFMDKGLKVALFGSKNDRDVTAEITALAPGVVDLAGQTRLED
AIDLIAAARLAVSNDSGLMHVAAGVGTPIVAVYGSTSPENTPPLAERRELVWLHLSCSPCHQKVCPLGHLNCLKTLEVGQ
VATAAERLLELPATA

Sequences:

>Translated_335_residues
MAESPTILVIGPRWVGDMVMAQCLFSALKELYPNAAIDVLAPAWAAPLVKRMPEIRQQIDLPLKPGALEFTIRRRFGRLL
RGRYDMAYVLPGSWKSALIPFFARIPRRFGHLREMRYGLLTDIVPLPDAVKRRTAQAYFSLAKGGSFRAPHLTVDAGNQA
ALLDRFGLAPRKFAALMPGAEFGPAKRWPSESYAGLARKFMDKGLKVALFGSKNDRDVTAEITALAPGVVDLAGQTRLED
AIDLIAAARLAVSNDSGLMHVAAGVGTPIVAVYGSTSPENTPPLAERRELVWLHLSCSPCHQKVCPLGHLNCLKTLEVGQ
VATAAERLLELPATA
>Mature_334_residues
AESPTILVIGPRWVGDMVMAQCLFSALKELYPNAAIDVLAPAWAAPLVKRMPEIRQQIDLPLKPGALEFTIRRRFGRLLR
GRYDMAYVLPGSWKSALIPFFARIPRRFGHLREMRYGLLTDIVPLPDAVKRRTAQAYFSLAKGGSFRAPHLTVDAGNQAA
LLDRFGLAPRKFAALMPGAEFGPAKRWPSESYAGLARKFMDKGLKVALFGSKNDRDVTAEITALAPGVVDLAGQTRLEDA
IDLIAAARLAVSNDSGLMHVAAGVGTPIVAVYGSTSPENTPPLAERRELVWLHLSCSPCHQKVCPLGHLNCLKTLEVGQV
ATAAERLLELPATA

Specific function: Lipopolysaccharide core biosynthesis. [C]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family [H]

Homologues:

Organism=Escherichia coli, GI1790050, Length=305, Percent_Identity=47.5409836065574, Blast_Score=260, Evalue=9e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002201
- InterPro:   IPR011910 [H]

Pfam domain/function: PF01075 Glyco_transf_9 [H]

EC number: 2.4.1.-

Molecular weight: Translated: 36440; Mature: 36309

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAESPTILVIGPRWVGDMVMAQCLFSALKELYPNAAIDVLAPAWAAPLVKRMPEIRQQID
CCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
LPLKPGALEFTIRRRFGRLLRGRYDMAYVLPGSWKSALIPFFARIPRRFGHLREMRYGLL
CCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TDIVPLPDAVKRRTAQAYFSLAKGGSFRAPHLTVDAGNQAALLDRFGLAPRKFAALMPGA
HHCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHCCCHHHHHHHCCCC
EFGPAKRWPSESYAGLARKFMDKGLKVALFGSKNDRDVTAEITALAPGVVDLAGQTRLED
CCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEEHHCCCHHHHCCCHHHHH
AIDLIAAARLAVSNDSGLMHVAAGVGTPIVAVYGSTSPENTPPLAERRELVWLHLSCSPC
HHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEECCCCCCCCCCHHHCCEEEEEEECCCHH
HQKVCPLGHLNCLKTLEVGQVATAAERLLELPATA
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AESPTILVIGPRWVGDMVMAQCLFSALKELYPNAAIDVLAPAWAAPLVKRMPEIRQQID
CCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
LPLKPGALEFTIRRRFGRLLRGRYDMAYVLPGSWKSALIPFFARIPRRFGHLREMRYGLL
CCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TDIVPLPDAVKRRTAQAYFSLAKGGSFRAPHLTVDAGNQAALLDRFGLAPRKFAALMPGA
HHCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHCCCHHHHHHHCCCC
EFGPAKRWPSESYAGLARKFMDKGLKVALFGSKNDRDVTAEITALAPGVVDLAGQTRLED
CCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEEHHCCCHHHHCCCHHHHH
AIDLIAAARLAVSNDSGLMHVAAGVGTPIVAVYGSTSPENTPPLAERRELVWLHLSCSPC
HHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEECCCCCCCCCCHHHCCEEEEEEECCCHH
HQKVCPLGHLNCLKTLEVGQVATAAERLLELPATA
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 2198271; 8478319 [H]