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The map label for this gene is hldD

Identifier: 13472311

GI number: 13472311

Start: 2075392

End: 2076342

Strand: Direct

Name: hldD

Synonym: mlr2565

Alternate gene names: 13472311

Gene position: 2075392-2076342 (Clockwise)

Preceding gene: 13472306

Following gene: 13472312

Centisome position: 29.5

GC content: 61.93

Gene sequence:

>951_bases
ATGATCATCGTCACGGGCGGCGCCGGCATGATCGGCTCCAACATCGTCGCCGCGCTCAATGCCGAGGGCCATGACGACAT
CCTGGTCGTCGATGATCTCACCGACGGCCACAAGATCGCCAATCTCGCCGATCTCAGCATTGCCGACTATCTCGACAAGG
ACGATTTTCTAACCCGCATCGAGGCCGGGGCGCTCGGCCGCATCGAGGCCGTCTTCCACCAGGGCGCCTGCTCAACCACC
ACCGAGTGGAACGGCAAGTTCATGATGGAGGTGAATTTTGCTTATTCAAAGCGGCTGCTGCATGCCTGCCAGGCGCCGCG
CGTGCCCTTCCTCTACGCCTCCTCGGCTTCTGTCTATGGCGGCGGTTCGGAGTTTCGCGAGGAGCCGGCGCTGGAGCGGC
CGCTCAATGTCTATGCCTATTCGAAGAAACTGTTCGACGACTATGTCAGGCGCACCGTCTTCGACACCGATCACTCGCAG
GTCGCCGGCCTGCGCTATTTCAACGTCTACGGCCCGCGAGAGGCCCACAAGGGCGCCATGGCCTCGGTCGCCTTCCATCT
GTTCAACCAGGTCGAGCGTGGCGAGAATCCGAGACTGTTCGGCGCCTATGACGGTTTCGGCCCCGGCGAACAGAGCCGCG
ACTTCATCCATGTCGGCGACGTCGCCGACGTCAATCTGTGGCTCTGGAAGCGAGGATCGAGCGGCATCTTCAACTGCGGC
ACCGGCCGCGCCCAGCCGTTCCGCGCCATCGCCGAAACCGTCATCGACACGCTGGGCAAGGGCGAGATCGAGTTCATCCC
CTTCCCCGACCACCTCAAGGGCAGCTACCAGAGCTTCACGCAAGCTGATATGTCCCGTTTGCGCGCGGCCGGGTATAATG
GCCAGTTCCGGACAGTGGAAACCGGTGTCAGAGACTATGTCGAATGGCTGAAAGCCCAACGATCCTCGTGA

Upstream 100 bases:

>100_bases
GGAAGCTTTGCGGCGCCGCTGTGACGCCTGCACCTGCTTTGTCGACAGCCCGGCTGGCCTCCCTTATAAGGACCGGAATC
GCAAGCAACCAGAGGCCTTC

Downstream 100 bases:

>100_bases
TCGGCCCACGCTGGGTGGGCGACATGGTGATGGCGCAGTGCCTGTTCTCGGCGCTGAAGGAACTCTATCCCAACGCCGCC
ATCGACGTGCTGGCACCGGC

Product: ADP-L-glycero-D-mannoheptose-6-epimerase

Products: NA

Alternate protein names: ADP-L-glycero-beta-D-manno-heptose-6-epimerase; ADP-glyceromanno-heptose 6-epimerase; ADP-hep 6-epimerase; AGME

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MIIVTGGAGMIGSNIVAALNAEGHDDILVVDDLTDGHKIANLADLSIADYLDKDDFLTRIEAGALGRIEAVFHQGACSTT
TEWNGKFMMEVNFAYSKRLLHACQAPRVPFLYASSASVYGGGSEFREEPALERPLNVYAYSKKLFDDYVRRTVFDTDHSQ
VAGLRYFNVYGPREAHKGAMASVAFHLFNQVERGENPRLFGAYDGFGPGEQSRDFIHVGDVADVNLWLWKRGSSGIFNCG
TGRAQPFRAIAETVIDTLGKGEIEFIPFPDHLKGSYQSFTQADMSRLRAAGYNGQFRTVETGVRDYVEWLKAQRSS

Sequences:

>Translated_316_residues
MIIVTGGAGMIGSNIVAALNAEGHDDILVVDDLTDGHKIANLADLSIADYLDKDDFLTRIEAGALGRIEAVFHQGACSTT
TEWNGKFMMEVNFAYSKRLLHACQAPRVPFLYASSASVYGGGSEFREEPALERPLNVYAYSKKLFDDYVRRTVFDTDHSQ
VAGLRYFNVYGPREAHKGAMASVAFHLFNQVERGENPRLFGAYDGFGPGEQSRDFIHVGDVADVNLWLWKRGSSGIFNCG
TGRAQPFRAIAETVIDTLGKGEIEFIPFPDHLKGSYQSFTQADMSRLRAAGYNGQFRTVETGVRDYVEWLKAQRSS
>Mature_316_residues
MIIVTGGAGMIGSNIVAALNAEGHDDILVVDDLTDGHKIANLADLSIADYLDKDDFLTRIEAGALGRIEAVFHQGACSTT
TEWNGKFMMEVNFAYSKRLLHACQAPRVPFLYASSASVYGGGSEFREEPALERPLNVYAYSKKLFDDYVRRTVFDTDHSQ
VAGLRYFNVYGPREAHKGAMASVAFHLFNQVERGENPRLFGAYDGFGPGEQSRDFIHVGDVADVNLWLWKRGSSGIFNCG
TGRAQPFRAIAETVIDTLGKGEIEFIPFPDHLKGSYQSFTQADMSRLRAAGYNGQFRTVETGVRDYVEWLKAQRSS

Specific function: Catalyzes the interconversion between ADP-D-glycero- beta-D-manno-heptose and ADP-L-glycero-beta-D-manno-heptose via an epimerization at carbon 6 of the heptose

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. HldD subfamily

Homologues:

Organism=Escherichia coli, GI1790049, Length=312, Percent_Identity=60.8974358974359, Blast_Score=388, Evalue=1e-109,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): HLDD_RHILO (Q98I52)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_103878.1
- ProteinModelPortal:   Q98I52
- SMR:   Q98I52
- GeneID:   1226539
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr2565
- NMPDR:   fig|266835.1.peg.1982
- HOGENOM:   HBG755066
- OMA:   GFIGSAM
- BRENDA:   5.1.3.20
- HAMAP:   MF_01601
- InterPro:   IPR001509
- InterPro:   IPR011912
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR10366:SF29
- TIGRFAMs:   TIGR02197

Pfam domain/function: PF01370 Epimerase

EC number: =5.1.3.20

Molecular weight: Translated: 34978; Mature: 34978

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: ACT_SITE 138-138 ACT_SITE 177-177 BINDING 38-38 BINDING 53-53 BINDING 142-142 BINDING 168-168 BINDING 169-169 BINDING 177-177 BINDING 179-179 BINDING 186-186 BINDING 213-213 BINDING 276-276

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIVTGGAGMIGSNIVAALNAEGHDDILVVDDLTDGHKIANLADLSIADYLDKDDFLTRI
CEEEECCCCCCCCCEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHH
EAGALGRIEAVFHQGACSTTTEWNGKFMMEVNFAYSKRLLHACQAPRVPFLYASSASVYG
CCCCCHHHHHHHHCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHCCCCCCEEEECCCEECC
GGSEFREEPALERPLNVYAYSKKLFDDYVRRTVFDTDHSQVAGLRYFNVYGPREAHKGAM
CCHHHHCCCHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCEEEEEECCCCHHHHHHH
ASVAFHLFNQVERGENPRLFGAYDGFGPGEQSRDFIHVGDVADVNLWLWKRGSSGIFNCG
HHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCEEEECCCCEEEEEEEECCCCCEEECC
TGRAQPFRAIAETVIDTLGKGEIEFIPFPDHLKGSYQSFTQADMSRLRAAGYNGQFRTVE
CCCCHHHHHHHHHHHHHCCCCCEEEEECCHHHCCHHHHHHHHHHHHHHHCCCCCCEEEHH
TGVRDYVEWLKAQRSS
HHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIIVTGGAGMIGSNIVAALNAEGHDDILVVDDLTDGHKIANLADLSIADYLDKDDFLTRI
CEEEECCCCCCCCCEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHH
EAGALGRIEAVFHQGACSTTTEWNGKFMMEVNFAYSKRLLHACQAPRVPFLYASSASVYG
CCCCCHHHHHHHHCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHCCCCCCEEEECCCEECC
GGSEFREEPALERPLNVYAYSKKLFDDYVRRTVFDTDHSQVAGLRYFNVYGPREAHKGAM
CCHHHHCCCHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCEEEEEECCCCHHHHHHH
ASVAFHLFNQVERGENPRLFGAYDGFGPGEQSRDFIHVGDVADVNLWLWKRGSSGIFNCG
HHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCEEEECCCCEEEEEEEECCCCCEEECC
TGRAQPFRAIAETVIDTLGKGEIEFIPFPDHLKGSYQSFTQADMSRLRAAGYNGQFRTVE
CCCCHHHHHHHHHHHHHCCCCCEEEEECCHHHCCHHHHHHHHHHHHHHHCCCCCCEEEHH
TGVRDYVEWLKAQRSS
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968