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The map label for this gene is fusA [C]

Identifier: 13472301

GI number: 13472301

Start: 2064613

End: 2066664

Strand: Direct

Name: fusA [C]

Synonym: mlr2549

Alternate gene names: 13472301

Gene position: 2064613-2066664 (Clockwise)

Preceding gene: 13472300

Following gene: 13472302

Centisome position: 29.34

GC content: 65.16

Gene sequence:

>2052_bases
ATGGGTACTCGCGCCGGAGGACGACGCACGGGACCGAAATGCATTGCCATAGTCGGTCCCTTTGCAAGCGGTAAGACGAC
ACTTCTCGAAGCCATATTGGCCCGTACGGGCGCCATCCCCCGCCAGAATCCCGTTTCCTCCGGCAGCACTGTCTCAGATC
ATTCGCCAGAGGCCCGCGCCCACGCCATGAGCGTCGAGGCGACGATCGCCACCACCGAATTCATGGGCGAGCAGATCACC
TTCGTCGATTGTCCCGGCTCGATCGAATTCTCCTTCGAGGCCGAGCCGGTGCTGGCCGCCTGCGACCTCGCAGTGGTTGT
GGCCGAGGCGGACGAAAAGAAGATCCCTGCCCTGCAACTCATCATGCGCAAGCTCGACGATCTCGGCGTGCCGCGCATCC
TCTTCCTCAATAAGGTCGACAAGGCGATCTCAGGCGTACGCGACACGCTGAAGATGCTGCAGCCGGCAAGCTCGGTGCCG
CTCCTGCTGCGCCAGATTCCGCTGCGCAAGGACGGCGTCGTCATCGGCTCGATCGATCTGGCGCTGGAGCGCGCCTACAT
CTACCGCGAATATGCCGAAAGCGAGGTGGCTCAGATACCGGGCGACGACAAGGCGCGCGAACTGGAAGCGCGCTTCTCCA
TGCTGGAAACACTGGCCGACCATGACGACCAGCTGATGGAACAGTTGCTCGAGGAGATCGAGCCGCCGAAGGACGCGATC
TTCGACGACCTCGCCGCCGATCTGCGCGAAGGCGTCGTGACGCCGGTGCTGATCGGCACCGCCGAAAAGGGCAACGGCGT
GCTGCGCCTCTTGAAGGCGATCCGCCACGACGCACCCGACATCGAGGCAACCCGCAAGCGGCTTGGCGCACCGGACGGCG
GCGCCACCGTGGTCCAGGTGATGAAGACCATCCACACGCCGCATGGCGGCAAGCTGTCGGTGTCGCGCATCCTGTCCGGC
CAGGTCGCCGACGGCTCCGAGCTGTGGCTGCCCGGCGGCGACACCGCCAAGGTTTCCGGCATCTACAAGATGCTCGGCAA
GGACCAGTTCAAGCTCACCGCCGCCAAGGCCGGTGACACCGTGGCGCTCGGCAAGCTGGACGAGGTCAAGACCGGCCAGA
CGCTGACCTCGGCCAAGGGTGGCACCAAACAGCTGTTCACGTTCGAGCCGCCGCAGCCGGTCTTCGCCTTCGCGCTGCGG
CCGAAGGAACGCAAGGACGAGGTCAAGATGTCGGCCGCCATCCAGCGGCTGGCCGAGGAAGATCCTTCGCTCAGCCTGCG
CCACAACCAGGACTCGGCCGAGACGGTGCTGTCAGGCCATGGCGAAATGCATCTGCGCGTCGTGCGCGAGCGGCTGGAGG
GCAAGAACCAGATCCCTGTCGAAGGCCACGCGCCCGCTGTGCCCTACCGCGAGACGATCCGCAAATCGGCGCAGCAGCGT
GGCCGCCACAAGAAGCAGTCGGGCGGCCATGGCCAGTTCGGCGATGTGGTGATCGAGATCAAGCCGATGCCGCGCGGCTC
CGGCTTCCAGTTCACCGACACCATCACCGGCGGCGTCGTGCCGAAGACCTATATCCAGTCGGTCGAGACAGGCATACGCG
ATTACCTGAAGACCGGCCCGCTCGGCTTCCCCGTCGTCGACGTCGCCGTCAACCTGTCGGACGGCTCCTACCATGCGGTG
GATTCCTCCGACATGGCCTTCCAGATGGCGGCGAAGCTGGCGATGAAGGAAGGCATGGCCGCCTGCTCGCCGGTGCTGCT
GGAGCCGGTGATGAAGGTCGAAATCGTCACGCCGTCCGACGCGACATCCAAGATCATCGCGCTGATCCCGCAGCGGCGTG
GCCAGATCCTCGGCTATGACGCGCGGCCGGGATGGCCGGGATGGGATGTCGTCGAGGCGACCATGCCGCAGGCCGAGATT
GGTGACCTGATCATCGAACTGCGCTCGGCGACCGCGGGCGTCGCCAGCTATCGCGCCGTCTTCGACCATATGGCCGAACT
CACCGGCCGTCTTGCCGACGAAGCAATGAATACCAACGGCAAGGCTGCCTGA

Upstream 100 bases:

>100_bases
GCGACGGTCGAACAGGCCGGCCGCCGCTCAGGACCGATTGACCAAGCCTGAACCATGCAAGCAGTCGGTCCCTAGGGAAA
CGCCTGAGTGGAGGTCAGCC

Downstream 100 bases:

>100_bases
TCCTGATCTCGACACATTGTCCCCTCGAACCTTGTTCGAGGGGACAAAAACAAAAAAACGACGCCCGGGGAGCCGGACGT
CGTTTTGTTTGCGGACCGTT

Product: elongation factor G

Products: GDP; phosphate

Alternate protein names: NA

Number of amino acids: Translated: 683; Mature: 682

Protein sequence:

>683_residues
MGTRAGGRRTGPKCIAIVGPFASGKTTLLEAILARTGAIPRQNPVSSGSTVSDHSPEARAHAMSVEATIATTEFMGEQIT
FVDCPGSIEFSFEAEPVLAACDLAVVVAEADEKKIPALQLIMRKLDDLGVPRILFLNKVDKAISGVRDTLKMLQPASSVP
LLLRQIPLRKDGVVIGSIDLALERAYIYREYAESEVAQIPGDDKARELEARFSMLETLADHDDQLMEQLLEEIEPPKDAI
FDDLAADLREGVVTPVLIGTAEKGNGVLRLLKAIRHDAPDIEATRKRLGAPDGGATVVQVMKTIHTPHGGKLSVSRILSG
QVADGSELWLPGGDTAKVSGIYKMLGKDQFKLTAAKAGDTVALGKLDEVKTGQTLTSAKGGTKQLFTFEPPQPVFAFALR
PKERKDEVKMSAAIQRLAEEDPSLSLRHNQDSAETVLSGHGEMHLRVVRERLEGKNQIPVEGHAPAVPYRETIRKSAQQR
GRHKKQSGGHGQFGDVVIEIKPMPRGSGFQFTDTITGGVVPKTYIQSVETGIRDYLKTGPLGFPVVDVAVNLSDGSYHAV
DSSDMAFQMAAKLAMKEGMAACSPVLLEPVMKVEIVTPSDATSKIIALIPQRRGQILGYDARPGWPGWDVVEATMPQAEI
GDLIIELRSATAGVASYRAVFDHMAELTGRLADEAMNTNGKAA

Sequences:

>Translated_683_residues
MGTRAGGRRTGPKCIAIVGPFASGKTTLLEAILARTGAIPRQNPVSSGSTVSDHSPEARAHAMSVEATIATTEFMGEQIT
FVDCPGSIEFSFEAEPVLAACDLAVVVAEADEKKIPALQLIMRKLDDLGVPRILFLNKVDKAISGVRDTLKMLQPASSVP
LLLRQIPLRKDGVVIGSIDLALERAYIYREYAESEVAQIPGDDKARELEARFSMLETLADHDDQLMEQLLEEIEPPKDAI
FDDLAADLREGVVTPVLIGTAEKGNGVLRLLKAIRHDAPDIEATRKRLGAPDGGATVVQVMKTIHTPHGGKLSVSRILSG
QVADGSELWLPGGDTAKVSGIYKMLGKDQFKLTAAKAGDTVALGKLDEVKTGQTLTSAKGGTKQLFTFEPPQPVFAFALR
PKERKDEVKMSAAIQRLAEEDPSLSLRHNQDSAETVLSGHGEMHLRVVRERLEGKNQIPVEGHAPAVPYRETIRKSAQQR
GRHKKQSGGHGQFGDVVIEIKPMPRGSGFQFTDTITGGVVPKTYIQSVETGIRDYLKTGPLGFPVVDVAVNLSDGSYHAV
DSSDMAFQMAAKLAMKEGMAACSPVLLEPVMKVEIVTPSDATSKIIALIPQRRGQILGYDARPGWPGWDVVEATMPQAEI
GDLIIELRSATAGVASYRAVFDHMAELTGRLADEAMNTNGKAA
>Mature_682_residues
GTRAGGRRTGPKCIAIVGPFASGKTTLLEAILARTGAIPRQNPVSSGSTVSDHSPEARAHAMSVEATIATTEFMGEQITF
VDCPGSIEFSFEAEPVLAACDLAVVVAEADEKKIPALQLIMRKLDDLGVPRILFLNKVDKAISGVRDTLKMLQPASSVPL
LLRQIPLRKDGVVIGSIDLALERAYIYREYAESEVAQIPGDDKARELEARFSMLETLADHDDQLMEQLLEEIEPPKDAIF
DDLAADLREGVVTPVLIGTAEKGNGVLRLLKAIRHDAPDIEATRKRLGAPDGGATVVQVMKTIHTPHGGKLSVSRILSGQ
VADGSELWLPGGDTAKVSGIYKMLGKDQFKLTAAKAGDTVALGKLDEVKTGQTLTSAKGGTKQLFTFEPPQPVFAFALRP
KERKDEVKMSAAIQRLAEEDPSLSLRHNQDSAETVLSGHGEMHLRVVRERLEGKNQIPVEGHAPAVPYRETIRKSAQQRG
RHKKQSGGHGQFGDVVIEIKPMPRGSGFQFTDTITGGVVPKTYIQSVETGIRDYLKTGPLGFPVVDVAVNLSDGSYHAVD
SSDMAFQMAAKLAMKEGMAACSPVLLEPVMKVEIVTPSDATSKIIALIPQRRGQILGYDARPGWPGWDVVEATMPQAEIG
DLIIELRSATAGVASYRAVFDHMAELTGRLADEAMNTNGKAA

Specific function: This Protein Promotes The GTP-Dependent Translocation Of The Nascent Protein Chain From The A-Site To The P-Site Of The Ribosome. [C]

COG id: COG0480

COG function: function code J; Translation elongation factors (GTPases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=673, Percent_Identity=28.8261515601783, Blast_Score=258, Evalue=1e-68,
Organism=Homo sapiens, GI19923640, Length=697, Percent_Identity=26.1119081779053, Blast_Score=193, Evalue=4e-49,
Organism=Homo sapiens, GI25306287, Length=674, Percent_Identity=25.8160237388724, Blast_Score=171, Evalue=2e-42,
Organism=Homo sapiens, GI25306283, Length=386, Percent_Identity=25.3886010362694, Blast_Score=101, Evalue=2e-21,
Organism=Escherichia coli, GI1789738, Length=714, Percent_Identity=32.6330532212885, Blast_Score=304, Evalue=1e-83,
Organism=Caenorhabditis elegans, GI17533571, Length=688, Percent_Identity=27.906976744186, Blast_Score=258, Evalue=6e-69,
Organism=Caenorhabditis elegans, GI17556745, Length=688, Percent_Identity=22.093023255814, Blast_Score=137, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6323098, Length=686, Percent_Identity=27.9883381924198, Blast_Score=239, Evalue=1e-63,
Organism=Saccharomyces cerevisiae, GI6322359, Length=515, Percent_Identity=27.7669902912621, Blast_Score=144, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6324707, Length=429, Percent_Identity=25.6410256410256, Blast_Score=97, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6320593, Length=429, Percent_Identity=25.6410256410256, Blast_Score=97, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24582462, Length=674, Percent_Identity=29.5252225519288, Blast_Score=263, Evalue=3e-70,
Organism=Drosophila melanogaster, GI221458488, Length=694, Percent_Identity=25.7925072046109, Blast_Score=180, Evalue=3e-45,
Organism=Drosophila melanogaster, GI24585709, Length=438, Percent_Identity=25.7990867579909, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24585711, Length=438, Percent_Identity=25.7990867579909, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24585713, Length=438, Percent_Identity=25.7990867579909, Blast_Score=89, Evalue=1e-17,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 7984 Molecules/Cell In: Growth Phase, Gl

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR000795
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR005517
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF03764 EFG_IV; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 73474; Mature: 73343

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTRAGGRRTGPKCIAIVGPFASGKTTLLEAILARTGAIPRQNPVSSGSTVSDHSPEARA
CCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHH
HAMSVEATIATTEFMGEQITFVDCPGSIEFSFEAEPVLAACDLAVVVAEADEKKIPALQL
HHEEEHHHHHHHHHCCCEEEEEECCCCEEEEECCCCHHHHCCEEEEEECCCCCCCHHHHH
IMRKLDDLGVPRILFLNKVDKAISGVRDTLKMLQPASSVPLLLRQIPLRKDGVVIGSIDL
HHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCEEEEEHHH
ALERAYIYREYAESEVAQIPGDDKARELEARFSMLETLADHDDQLMEQLLEEIEPPKDAI
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHH
FDDLAADLREGVVTPVLIGTAEKGNGVLRLLKAIRHDAPDIEATRKRLGAPDGGATVVQV
HHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHH
MKTIHTPHGGKLSVSRILSGQVADGSELWLPGGDTAKVSGIYKMLGKDQFKLTAAKAGDT
HHHHCCCCCCCEEHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCE
VALGKLDEVKTGQTLTSAKGGTKQLFTFEPPQPVFAFALRPKERKDEVKMSAAIQRLAEE
EEECCCHHCCCCCEECCCCCCCCEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHC
DPSLSLRHNQDSAETVLSGHGEMHLRVVRERLEGKNQIPVEGHAPAVPYRETIRKSAQQR
CCCEEEECCCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHH
GRHKKQSGGHGQFGDVVIEIKPMPRGSGFQFTDTITGGVVPKTYIQSVETGIRDYLKTGP
CCHHHCCCCCCCCCCEEEEEEECCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHCC
LGFPVVDVAVNLSDGSYHAVDSSDMAFQMAAKLAMKEGMAACSPVLLEPVMKVEIVTPSD
CCCCEEEEEEEECCCCEEECCCCHHHHHHHHHHHHHHCHHHHCHHHHCCCEEEEEECCCC
ATSKIIALIPQRRGQILGYDARPGWPGWDVVEATMPQAEIGDLIIELRSATAGVASYRAV
CHHHHHHCCCCCCCCEEEECCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
FDHMAELTGRLADEAMNTNGKAA
HHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
GTRAGGRRTGPKCIAIVGPFASGKTTLLEAILARTGAIPRQNPVSSGSTVSDHSPEARA
CCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHH
HAMSVEATIATTEFMGEQITFVDCPGSIEFSFEAEPVLAACDLAVVVAEADEKKIPALQL
HHEEEHHHHHHHHHCCCEEEEEECCCCEEEEECCCCHHHHCCEEEEEECCCCCCCHHHHH
IMRKLDDLGVPRILFLNKVDKAISGVRDTLKMLQPASSVPLLLRQIPLRKDGVVIGSIDL
HHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCEEEEEHHH
ALERAYIYREYAESEVAQIPGDDKARELEARFSMLETLADHDDQLMEQLLEEIEPPKDAI
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHH
FDDLAADLREGVVTPVLIGTAEKGNGVLRLLKAIRHDAPDIEATRKRLGAPDGGATVVQV
HHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHH
MKTIHTPHGGKLSVSRILSGQVADGSELWLPGGDTAKVSGIYKMLGKDQFKLTAAKAGDT
HHHHCCCCCCCEEHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCE
VALGKLDEVKTGQTLTSAKGGTKQLFTFEPPQPVFAFALRPKERKDEVKMSAAIQRLAEE
EEECCCHHCCCCCEECCCCCCCCEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHC
DPSLSLRHNQDSAETVLSGHGEMHLRVVRERLEGKNQIPVEGHAPAVPYRETIRKSAQQR
CCCEEEECCCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHH
GRHKKQSGGHGQFGDVVIEIKPMPRGSGFQFTDTITGGVVPKTYIQSVETGIRDYLKTGP
CCHHHCCCCCCCCCCEEEEEEECCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHCC
LGFPVVDVAVNLSDGSYHAVDSSDMAFQMAAKLAMKEGMAACSPVLLEPVMKVEIVTPSD
CCCCEEEEEEEECCCCEEECCCCHHHHHHHHHHHHHHCHHHHCHHHHCCCEEEEEECCCC
ATSKIIALIPQRRGQILGYDARPGWPGWDVVEATMPQAEIGDLIIELRSATAGVASYRAV
CHHHHHHCCCCCCCCEEEECCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
FDHMAELTGRLADEAMNTNGKAA
HHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]