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The map label for this gene is carA

Identifier: 13472256

GI number: 13472256

Start: 2020677

End: 2021873

Strand: Direct

Name: carA

Synonym: mlr2489

Alternate gene names: 13472256

Gene position: 2020677-2021873 (Clockwise)

Preceding gene: 13472253

Following gene: 13472260

Centisome position: 28.72

GC content: 66.58

Gene sequence:

>1197_bases
ATGACGCCCGCCTGGGCCACCGAAAAGCCGACCGCCCTTCTGGTGCTGGCCGACGGCACCGTCATCGAGGGTCGGGGCCT
CGGCGCCACCGGTTCCGCCGTCGCCGAAGTCTGCTTCAACACCGCGCTCACCGGCTACCAGGAGATCCTCACCGACCCGT
CCTATGCCGGCCAGATCGTCACCTTCACCTTCCCGCATATCGGCAATATCGGCACCAATGGCGAGGACATCGAGGACCTC
AATCCGGCCGCCCGCGCCGGCGCTGTCGGCGCCGTGTTCAAGGCCGATGTCACCAACCCGTCCAACTACCGCGCCGCCGG
CCACCTCGACCAGTGGCTGAAGAAGCGCGGCATCGTCGCACTCTCGGGCATCGACACCCGCGCGCTGACCGCGCTGATCC
GCGAAAAGGGCATGCCCAATGCCGTCATCGCGCATGCGCCGGACGGTGTCTTCGACCTCGACGATCTGAAGCAACGCGCC
GCCGCATGGTCGGGCCTGATCGGGCTCGACCTCGCCAAGGAAGTCACGTCGGGCCAGTCCTCGGTCTGGCGCGAGACGCC
TTGGGTGTGGAACGAAGGTTTTGGCGAGCAGGCCGAGCCGTCGCTGCACGTCGTGGCCATCGACTACGGCGTCAAGCGCA
ACATATTGCGCCTGCTTGCCGGCCTCGGCGCCAAGGTGACTGTCGTTCCGGCAAACACCGGTTCGGAAGAAATCCTCGCC
ATGCAGCCCGACGGCATCTTCCTCTCCAACGGCCCCGGCGACCCGGAAGCCACCGGCGACTATGCCGTGCCGGTCATCCA
GGATCTGCTGAAGACCGACATTCCGGTGTTCGGCATCTGCCTCGGCCACCAGATGCTGGCGCTGGCGCTGGGCGGCAGGA
CCGCCAAGATGCACCAGGGCCATCACGGCGCCAACCATCCGGTCAAGGACCACACCACCGGCAAGGTCGAGATCGTCTCG
ATGAACCACGGCTTTGCCGTCGACGCCGACTCGCTGCCAGAGGGCGTCGAGGAAACCCATGTCTCGCTGTTCGACGGCTC
GAACTGCGGCATCGCGCTCACCGGCCGTCCGGTGTTCTCGGTCCAGCATCACCCCGAAGCCTCGCCCGGCCCACAGGATT
CCCACTATCTGTTCCGCCGCTTCGTCAACCTGATCCGCGAGAAGCGCGGCGAGGAACAGCTGGCCGAGCGGGTCTGA

Upstream 100 bases:

>100_bases
TTGACGTGCTGAGGCTCGAGAAATCGCTGCCACGCCGTTTGCTGCGCGGATGGTCCATCATCGAAGCGCATAGCCTGATA
GGAGTGCCGCCATGGCCGAG

Downstream 100 bases:

>100_bases
CCTTACGCCACTGGCCGGCCTTCACTCGCCTCCAGTATGGCGAACCATTGCTGGCGGTCGAGACTGATCTCCAGCGCCTT
GACCATGGCCGCCAGCCGTT

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MTPAWATEKPTALLVLADGTVIEGRGLGATGSAVAEVCFNTALTGYQEILTDPSYAGQIVTFTFPHIGNIGTNGEDIEDL
NPAARAGAVGAVFKADVTNPSNYRAAGHLDQWLKKRGIVALSGIDTRALTALIREKGMPNAVIAHAPDGVFDLDDLKQRA
AAWSGLIGLDLAKEVTSGQSSVWRETPWVWNEGFGEQAEPSLHVVAIDYGVKRNILRLLAGLGAKVTVVPANTGSEEILA
MQPDGIFLSNGPGDPEATGDYAVPVIQDLLKTDIPVFGICLGHQMLALALGGRTAKMHQGHHGANHPVKDHTTGKVEIVS
MNHGFAVDADSLPEGVEETHVSLFDGSNCGIALTGRPVFSVQHHPEASPGPQDSHYLFRRFVNLIREKRGEEQLAERV

Sequences:

>Translated_398_residues
MTPAWATEKPTALLVLADGTVIEGRGLGATGSAVAEVCFNTALTGYQEILTDPSYAGQIVTFTFPHIGNIGTNGEDIEDL
NPAARAGAVGAVFKADVTNPSNYRAAGHLDQWLKKRGIVALSGIDTRALTALIREKGMPNAVIAHAPDGVFDLDDLKQRA
AAWSGLIGLDLAKEVTSGQSSVWRETPWVWNEGFGEQAEPSLHVVAIDYGVKRNILRLLAGLGAKVTVVPANTGSEEILA
MQPDGIFLSNGPGDPEATGDYAVPVIQDLLKTDIPVFGICLGHQMLALALGGRTAKMHQGHHGANHPVKDHTTGKVEIVS
MNHGFAVDADSLPEGVEETHVSLFDGSNCGIALTGRPVFSVQHHPEASPGPQDSHYLFRRFVNLIREKRGEEQLAERV
>Mature_397_residues
TPAWATEKPTALLVLADGTVIEGRGLGATGSAVAEVCFNTALTGYQEILTDPSYAGQIVTFTFPHIGNIGTNGEDIEDLN
PAARAGAVGAVFKADVTNPSNYRAAGHLDQWLKKRGIVALSGIDTRALTALIREKGMPNAVIAHAPDGVFDLDDLKQRAA
AWSGLIGLDLAKEVTSGQSSVWRETPWVWNEGFGEQAEPSLHVVAIDYGVKRNILRLLAGLGAKVTVVPANTGSEEILAM
QPDGIFLSNGPGDPEATGDYAVPVIQDLLKTDIPVFGICLGHQMLALALGGRTAKMHQGHHGANHPVKDHTTGKVEIVSM
NHGFAVDADSLPEGVEETHVSLFDGSNCGIALTGRPVFSVQHHPEASPGPQDSHYLFRRFVNLIREKRGEEQLAERV

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI18105007, Length=402, Percent_Identity=37.8109452736318, Blast_Score=228, Evalue=7e-60,
Organism=Homo sapiens, GI169790915, Length=391, Percent_Identity=36.3171355498721, Blast_Score=205, Evalue=5e-53,
Organism=Homo sapiens, GI21361331, Length=391, Percent_Identity=36.3171355498721, Blast_Score=205, Evalue=6e-53,
Organism=Escherichia coli, GI1786215, Length=384, Percent_Identity=53.90625, Blast_Score=391, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI193204318, Length=393, Percent_Identity=36.8956743002545, Blast_Score=233, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6322331, Length=412, Percent_Identity=34.7087378640777, Blast_Score=228, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6324878, Length=392, Percent_Identity=37.2448979591837, Blast_Score=224, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6322638, Length=191, Percent_Identity=30.8900523560209, Blast_Score=69, Evalue=9e-13,
Organism=Drosophila melanogaster, GI45555749, Length=392, Percent_Identity=36.2244897959184, Blast_Score=210, Evalue=1e-54,
Organism=Drosophila melanogaster, GI24642586, Length=392, Percent_Identity=36.2244897959184, Blast_Score=209, Evalue=2e-54,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): CARA_RHILO (Q98IA7)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_103823.1
- ProteinModelPortal:   Q98IA7
- GeneID:   1226484
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr2489
- NMPDR:   fig|266835.1.peg.1927
- HOGENOM:   HBG286341
- OMA:   FTYPELG
- ProtClustDB:   PRK12564
- BRENDA:   6.3.5.5
- HAMAP:   MF_01209_B
- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- PANTHER:   PTHR11405:SF4
- PRINTS:   PR00097
- PRINTS:   PR00099
- PRINTS:   PR00096
- TIGRFAMs:   TIGR01368

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase; SSF52021 CP_synthsmall

EC number: =6.3.5.5

Molecular weight: Translated: 42345; Mature: 42214

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 280-280 ACT_SITE 364-364 ACT_SITE 366-366

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPAWATEKPTALLVLADGTVIEGRGLGATGSAVAEVCFNTALTGYQEILTDPSYAGQIV
CCCCCCCCCCCEEEEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
TFTFPHIGNIGTNGEDIEDLNPAARAGAVGAVFKADVTNPSNYRAAGHLDQWLKKRGIVA
EEECCCCCCCCCCCCCHHHCCHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCEE
LSGIDTRALTALIREKGMPNAVIAHAPDGVFDLDDLKQRAAAWSGLIGLDLAKEVTSGQS
EECCCHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCH
SVWRETPWVWNEGFGEQAEPSLHVVAIDYGVKRNILRLLAGLGAKVTVVPANTGSEEILA
HHHHCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCEEEEEECCCCCCCEEE
MQPDGIFLSNGPGDPEATGDYAVPVIQDLLKTDIPVFGICLGHQMLALALGGRTAKMHQG
ECCCCEEEECCCCCCCCCCCEEHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCHHHCCC
HHGANHPVKDHTTGKVEIVSMNHGFAVDADSLPEGVEETHVSLFDGSNCGIALTGRPVFS
CCCCCCCCCCCCCCEEEEEEECCCEEECCCCCCCCHHHHEEEEEECCCCCEEEECCEEEE
VQHHPEASPGPQDSHYLFRRFVNLIREKRGEEQLAERV
EECCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHCC
>Mature Secondary Structure 
TPAWATEKPTALLVLADGTVIEGRGLGATGSAVAEVCFNTALTGYQEILTDPSYAGQIV
CCCCCCCCCCEEEEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
TFTFPHIGNIGTNGEDIEDLNPAARAGAVGAVFKADVTNPSNYRAAGHLDQWLKKRGIVA
EEECCCCCCCCCCCCCHHHCCHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCEE
LSGIDTRALTALIREKGMPNAVIAHAPDGVFDLDDLKQRAAAWSGLIGLDLAKEVTSGQS
EECCCHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCH
SVWRETPWVWNEGFGEQAEPSLHVVAIDYGVKRNILRLLAGLGAKVTVVPANTGSEEILA
HHHHCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCEEEEEECCCCCCCEEE
MQPDGIFLSNGPGDPEATGDYAVPVIQDLLKTDIPVFGICLGHQMLALALGGRTAKMHQG
ECCCCEEEECCCCCCCCCCCEEHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCHHHCCC
HHGANHPVKDHTTGKVEIVSMNHGFAVDADSLPEGVEETHVSLFDGSNCGIALTGRPVFS
CCCCCCCCCCCCCCEEEEEEECCCEEECCCCCCCCHHHHEEEEEECCCCCEEEECCEEEE
VQHHPEASPGPQDSHYLFRRFVNLIREKRGEEQLAERV
EECCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968