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The map label for this gene is xthA [H]

Identifier: 13471939

GI number: 13471939

Start: 1693030

End: 1693800

Strand: Reverse

Name: xthA [H]

Synonym: mll2070

Alternate gene names: 13471939

Gene position: 1693800-1693030 (Counterclockwise)

Preceding gene: 13471940

Following gene: 13471938

Centisome position: 24.07

GC content: 66.93

Gene sequence:

>771_bases
ATGAAACTCGCCACCTTCAACATCAACAACATCAACAGCCGGCTGGAAAACCTGCTGGCCTGGCTGGCGGCGGCAAAGCC
CGATGTCGTCTGCCTGCAGGAACTCAAGGCGCGCGACATGCAGTTTCCGCGCACCGCGCTTGCCGATGCCGGCTATGGCG
CGGTGTGGAAGGGCGAGCCGACATGGAACGGCGTCGCCATCCTGGCGCGCGGCGCGGAGCCGGTGCTGACCCGCGATGCC
TTGCCCGGCGACGATGCCGACCGGCAAAGCCGCTATATCGAGGCGGCGGTCGACGGCATCGTCATCGCTTGCCTCTATGC
GCCGAACGGCAATCCGCAGCCGGGGCCGAAATTCCAGTACAAGCTTGCCTGGCACGCGCGGCTGGAGGCGCATGCGGCGC
AGCTTCTCGACACCGGCCTGCCGGTCGTGCTGGCCGGCGACTACAACATCGTGCCCGAGCCGCGCGACATCTATGCCACG
CGCTCCTATGACGACAACGCGCTGGTGCAGCCGGAAAGCCGCGCCGCCTTCGCCGCCCTCATCGAGCAGGGCTGGGTCGA
CGCGCTGCGAAAAATCTATCCGAAAGAGACGCTCTACACCTTCTGGGACTATCGGCGGAACCGCTGGCCGCGCGATGCGG
GCCTCAGGCTCGACCACATCTTGCTGTCGAAGACGCTCGCCCGCCGGCTGAAAGGGGCGGGTATCGACCGCGACGTGCGC
GGTGAGGACGGCGCCAGCGACCACGCGCCTGTCTGGGTGGAGCTGAAGTAG

Upstream 100 bases:

>100_bases
CCGCGCAAGGGCATTTCGGGATTCTCCACCGCCGATCATCTGAAGACGCTGGCGGAGACGGTGAAGGCTTGGGCAGGCCG
GATCGCCTCCTGATCCAACC

Downstream 100 bases:

>100_bases
GTCGCGCCCAACGAAGCAAGGAATGTCCAGCCATTTCCGTGCGTTCGCGCCCACGCGGCATTCGCACCGGCGGCCACTCG
ACTCCGCCGCGGCCTTGCGT

Product: exonuclease

Products: NA

Alternate protein names: EXO III; Exonuclease III [H]

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MKLATFNINNINSRLENLLAWLAAAKPDVVCLQELKARDMQFPRTALADAGYGAVWKGEPTWNGVAILARGAEPVLTRDA
LPGDDADRQSRYIEAAVDGIVIACLYAPNGNPQPGPKFQYKLAWHARLEAHAAQLLDTGLPVVLAGDYNIVPEPRDIYAT
RSYDDNALVQPESRAAFAALIEQGWVDALRKIYPKETLYTFWDYRRNRWPRDAGLRLDHILLSKTLARRLKGAGIDRDVR
GEDGASDHAPVWVELK

Sequences:

>Translated_256_residues
MKLATFNINNINSRLENLLAWLAAAKPDVVCLQELKARDMQFPRTALADAGYGAVWKGEPTWNGVAILARGAEPVLTRDA
LPGDDADRQSRYIEAAVDGIVIACLYAPNGNPQPGPKFQYKLAWHARLEAHAAQLLDTGLPVVLAGDYNIVPEPRDIYAT
RSYDDNALVQPESRAAFAALIEQGWVDALRKIYPKETLYTFWDYRRNRWPRDAGLRLDHILLSKTLARRLKGAGIDRDVR
GEDGASDHAPVWVELK
>Mature_256_residues
MKLATFNINNINSRLENLLAWLAAAKPDVVCLQELKARDMQFPRTALADAGYGAVWKGEPTWNGVAILARGAEPVLTRDA
LPGDDADRQSRYIEAAVDGIVIACLYAPNGNPQPGPKFQYKLAWHARLEAHAAQLLDTGLPVVLAGDYNIVPEPRDIYAT
RSYDDNALVQPESRAAFAALIEQGWVDALRKIYPKETLYTFWDYRRNRWPRDAGLRLDHILLSKTLARRLKGAGIDRDVR
GEDGASDHAPVWVELK

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction [H]

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=262, Percent_Identity=27.0992366412214, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI18375503, Length=262, Percent_Identity=27.0992366412214, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI18375501, Length=262, Percent_Identity=27.0992366412214, Blast_Score=102, Evalue=4e-22,
Organism=Escherichia coli, GI1788046, Length=268, Percent_Identity=32.4626865671642, Blast_Score=131, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI71989536, Length=246, Percent_Identity=25.2032520325203, Blast_Score=78, Evalue=4e-15,
Organism=Drosophila melanogaster, GI221330655, Length=261, Percent_Identity=28.3524904214559, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI17136678, Length=261, Percent_Identity=28.3524904214559, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 28542; Mature: 28542

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLATFNINNINSRLENLLAWLAAAKPDVVCLQELKARDMQFPRTALADAGYGAVWKGEP
CEEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHCCCCCCEECCCC
TWNGVAILARGAEPVLTRDALPGDDADRQSRYIEAAVDGIVIACLYAPNGNPQPGPKFQY
CCCCEEEEECCCCCCEECCCCCCCCCHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCEEE
KLAWHARLEAHAAQLLDTGLPVVLAGDYNIVPEPRDIYATRSYDDNALVQPESRAAFAAL
EEEEHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHEEEECCCCCCCEECCCHHHHHHHH
IEQGWVDALRKIYPKETLYTFWDYRRNRWPRDAGLRLDHILLSKTLARRLKGAGIDRDVR
HHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
GEDGASDHAPVWVELK
CCCCCCCCCCEEEEEC
>Mature Secondary Structure
MKLATFNINNINSRLENLLAWLAAAKPDVVCLQELKARDMQFPRTALADAGYGAVWKGEP
CEEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHCCCCCCEECCCC
TWNGVAILARGAEPVLTRDALPGDDADRQSRYIEAAVDGIVIACLYAPNGNPQPGPKFQY
CCCCEEEEECCCCCCEECCCCCCCCCHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCEEE
KLAWHARLEAHAAQLLDTGLPVVLAGDYNIVPEPRDIYATRSYDDNALVQPESRAAFAAL
EEEEHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHEEEECCCCCCCEECCCHHHHHHHH
IEQGWVDALRKIYPKETLYTFWDYRRNRWPRDAGLRLDHILLSKTLARRLKGAGIDRDVR
HHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
GEDGASDHAPVWVELK
CCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]