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The map label for this gene is yafC [C]

Identifier: 13471779

GI number: 13471779

Start: 1536259

End: 1537161

Strand: Reverse

Name: yafC [C]

Synonym: mll1863

Alternate gene names: 13471779

Gene position: 1537161-1536259 (Counterclockwise)

Preceding gene: 13471781

Following gene: 13471774

Centisome position: 21.85

GC content: 64.01

Gene sequence:

>903_bases
ATGCTAAAGCTTGAAGCCGCCGCCGCGTTTGTCGCCGTCACGGAATCCGGCTCGATCAGCGAGGCGGCCAGGCGCATGGG
CCTGTCCAAATCTGTCATCAGCGAGCGGCTGTCGGAACTCGAGCGCAGCCTTGGCACGAGATTGCTGGACCGCACCACCC
GCAAACTCTTCATCACCGAAGCGGGGCGCGGTTTTTACGAGCGCGCCAAGCGCATCATGCAGGAGGTGGCCGATGCGAAA
GCCGAAATCGCCGAGGATCGCGGCGAACTCGCCGGACCGCTCAGGATATCCGCGCCGACCAGTTTCGGCATCCTCCATCT
CGGCCCGGCGCTGTACGGCTTCCTGGCCAGGCACCCGGGGATCGAACTGACGCTTGATGTCGACGATCGTTTCGTCAGCA
TGGTGGCCGATGGCTACGATGCCATTGTGCGTCACGGGCCGGTCGTCGATGGCGGACCTGTCATCGTCAAGAAGCTGGCT
TCCAGCCGTCGCTTCCTGGTGGCCTCGCCGGACTATGTCGAGCGGTTCGGATTGCCGGCGACCACCGACGACCTGAAACG
CCACAAGGGCATCATCTATTCCATCCGCGGTGCCGCCGATTGGCGGTTCAAGATTTCGCGGCGGCTGGTGACGATCCGCC
CGGTAACCGCGTTGCGCGTCAACAATGGGATCTTGATGCGCGACGCCGCGCTTGCCGGCCTCGGCCTGGCGCTGCTCCCC
GCCTATTTCATCCGGACCGAGATCGCCGACAAGCGATTGACGGTCATCGATGTCGGCGCCGAGCCTGAAGGGGCCACCAT
CTATATCGCCTGTCCCGAGGACAGGCGCGGATCCGCCAAACTGCGAGCGCTCACCGCATGGCTACGCGATGCCTTTGGCG
ATCCGCCCTATTGGGAGACGTGA

Upstream 100 bases:

>100_bases
TGTCACCGCCGCGTGTATCGCGGTCCTTTCAACTATGGCACCGTGCCCTTGGTTTCGTTAGGCGTCGGTCGCCTGATTGT
GAATTCGGGAAAGCCGAACA

Downstream 100 bases:

>100_bases
GCCGGCACTTCACCTCGCGTCGTGGAAGATGATGCCGACCGTGTGGCGCATGCCTGAGCGCAGGCGGCTGACGCCGTGGC
GCAGGTTGACGCGGTAGTTG

Product: transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MLKLEAAAAFVAVTESGSISEAARRMGLSKSVISERLSELERSLGTRLLDRTTRKLFITEAGRGFYERAKRIMQEVADAK
AEIAEDRGELAGPLRISAPTSFGILHLGPALYGFLARHPGIELTLDVDDRFVSMVADGYDAIVRHGPVVDGGPVIVKKLA
SSRRFLVASPDYVERFGLPATTDDLKRHKGIIYSIRGAADWRFKISRRLVTIRPVTALRVNNGILMRDAALAGLGLALLP
AYFIRTEIADKRLTVIDVGAEPEGATIYIACPEDRRGSAKLRALTAWLRDAFGDPPYWET

Sequences:

>Translated_300_residues
MLKLEAAAAFVAVTESGSISEAARRMGLSKSVISERLSELERSLGTRLLDRTTRKLFITEAGRGFYERAKRIMQEVADAK
AEIAEDRGELAGPLRISAPTSFGILHLGPALYGFLARHPGIELTLDVDDRFVSMVADGYDAIVRHGPVVDGGPVIVKKLA
SSRRFLVASPDYVERFGLPATTDDLKRHKGIIYSIRGAADWRFKISRRLVTIRPVTALRVNNGILMRDAALAGLGLALLP
AYFIRTEIADKRLTVIDVGAEPEGATIYIACPEDRRGSAKLRALTAWLRDAFGDPPYWET
>Mature_300_residues
MLKLEAAAAFVAVTESGSISEAARRMGLSKSVISERLSELERSLGTRLLDRTTRKLFITEAGRGFYERAKRIMQEVADAK
AEIAEDRGELAGPLRISAPTSFGILHLGPALYGFLARHPGIELTLDVDDRFVSMVADGYDAIVRHGPVVDGGPVIVKKLA
SSRRFLVASPDYVERFGLPATTDDLKRHKGIIYSIRGAADWRFKISRRLVTIRPVTALRVNNGILMRDAALAGLGLALLP
AYFIRTEIADKRLTVIDVGAEPEGATIYIACPEDRRGSAKLRALTAWLRDAFGDPPYWET

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=300, Percent_Identity=34, Blast_Score=147, Evalue=6e-37,
Organism=Escherichia coli, GI145693193, Length=291, Percent_Identity=32.6460481099656, Blast_Score=144, Evalue=8e-36,
Organism=Escherichia coli, GI87081978, Length=289, Percent_Identity=31.1418685121107, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1789639, Length=296, Percent_Identity=27.027027027027, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1787128, Length=296, Percent_Identity=28.3783783783784, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI1787589, Length=275, Percent_Identity=30.9090909090909, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI1789440, Length=294, Percent_Identity=25.5102040816327, Blast_Score=110, Evalue=8e-26,
Organism=Escherichia coli, GI1786448, Length=291, Percent_Identity=25.7731958762887, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI157672245, Length=158, Percent_Identity=31.6455696202532, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1789173, Length=291, Percent_Identity=25.4295532646048, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787601, Length=136, Percent_Identity=29.4117647058824, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788706, Length=308, Percent_Identity=25.3246753246753, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788748, Length=152, Percent_Identity=32.2368421052632, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1787806, Length=289, Percent_Identity=24.9134948096886, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788481, Length=128, Percent_Identity=28.90625, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32920; Mature: 32920

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: PS50931 HTH_LYSR ; PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKLEAAAAFVAVTESGSISEAARRMGLSKSVISERLSELERSLGTRLLDRTTRKLFITE
CEEECCEEEEEEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEE
AGRGFYERAKRIMQEVADAKAEIAEDRGELAGPLRISAPTSFGILHLGPALYGFLARHPG
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCC
IELTLDVDDRFVSMVADGYDAIVRHGPVVDGGPVIVKKLASSRRFLVASPDYVERFGLPA
CEEEEECCHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHCCCEEEEECCHHHHHCCCCC
TTDDLKRHKGIIYSIRGAADWRFKISRRLVTIRPVTALRVNNGILMRDAALAGLGLALLP
CHHHHHHCCCEEEEECCCCCEEEEEECEEEEEECEEEEEECCCEEEEHHHHHHHHHHHHH
AYFIRTEIADKRLTVIDVGAEPEGATIYIACPEDRRGSAKLRALTAWLRDAFGDPPYWET
HHHHHHHHCCCEEEEEECCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MLKLEAAAAFVAVTESGSISEAARRMGLSKSVISERLSELERSLGTRLLDRTTRKLFITE
CEEECCEEEEEEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEE
AGRGFYERAKRIMQEVADAKAEIAEDRGELAGPLRISAPTSFGILHLGPALYGFLARHPG
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCC
IELTLDVDDRFVSMVADGYDAIVRHGPVVDGGPVIVKKLASSRRFLVASPDYVERFGLPA
CEEEEECCHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHCCCEEEEECCHHHHHCCCCC
TTDDLKRHKGIIYSIRGAADWRFKISRRLVTIRPVTALRVNNGILMRDAALAGLGLALLP
CHHHHHHCCCEEEEECCCCCEEEEEECEEEEEECEEEEEECCCEEEEHHHHHHHHHHHHH
AYFIRTEIADKRLTVIDVGAEPEGATIYIACPEDRRGSAKLRALTAWLRDAFGDPPYWET
HHHHHHHHCCCEEEEEECCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]