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The map label for this gene is ddl

Identifier: 13471742

GI number: 13471742

Start: 1500630

End: 1501703

Strand: Reverse

Name: ddl

Synonym: mll1812

Alternate gene names: 13471742

Gene position: 1501703-1500630 (Counterclockwise)

Preceding gene: 13471743

Following gene: 13471741

Centisome position: 21.34

GC content: 66.85

Gene sequence:

>1074_bases
ATGAAGGAAAAAACAAGGGTCGCCATCCTCTATGGCGGGCGTTCGGCCGAGCATGACGTGTCGCGGCTGTCGGCCGCCAA
TGTGCTGAAGGCGATCGACCGGACGCGCTACGAGGTGGTGCCGATCGCCATTTCCAGGGACGGCAGATGGCGGCTGCAGC
CTTCGTCCGAGGCTGGCGCCGACGGGGCAGGGGCTCAGGTGCCGGAGGATGGCGTGGAGGTCGCGCTGCTGCCCGGCGGC
AAGGGCCGGCTGGTGGCTCTCACCAAGAATGGCGAGCAGCCCGACCCTGTCGATGTCGTCTTTCCCGTGCTGCATGGGCC
GTTCGGCGAGGACGGTTCGGTGCAAGGCTATGCCGAGGTTGCCGACGTCGCCTATGTCGGCTGCGGCATCCTGGCTTCGG
CGGCTGCCATGGACAAGGATGTGGCCAAGCGGCTGCTGCGCGAGGCGGGGCTTGCCGTGGCCCGCTCGGTCACCGTGCTG
AAAGGCGACGTCGGTTCGTTCCAGGAAATTGCCGGGGCGCTCGGGCTGCCCTTCTTCGCCAAGCCGGCGCGCCAGGGCTC
GTCCTTCGGGGTGAGCAAGGTGCATGACAGGGATGGTTTCGAGCAGGCCGTCGAAACGGCTTTGCGCTATGACAGCAAGG
CGCTGATCGAGGAGTTCGTCGACGGCCGCGAGATCGAGTGCTCGGTGCTGGAGCGGGCCGACGGTTCGCTGACGGTTTCG
CCGCCAGGCGAGATCATTCCGGCCGACAAGCATGGGTTCTACACCTACGAAGCGAAGTATTTCGACGCCGAAGGCGCGGT
GGTGAAGGTGCCCGCCGATGTCCCGGCGGATGTTGTTGCCAGGACGAGCGAGATGGCCGCGCGGGCTTTCCGGGCGCTCG
GCTGCGAGGCCATGGCGCGCGTCGACTTCTTCCTGCGCGCCGACGGTTCGCTGCTGGTCAACGAAGTCAACACGCTGCCC
GGCTTCACCGACATCTCGATGTACGCCAAGGCGCTGGCGGCGGCCGGCATCGGCTATAGCAAGGTGATCGACGTGCTGAT
CGAGCACGCGCTGGCAAGGCACCGGGCGCGCTGA

Upstream 100 bases:

>100_bases
GCGTCACACAGGCAAACGCAGTGTTGGCGCCAGCTGATTTCGATCGTTTCTTTGCCAATCGCGGCATTGCCATCACCATT
TGATTTTTTTGGGGATTTCA

Downstream 100 bases:

>100_bases
TCCGACCTGTGCAGCGTCACGCCAAGCTTGCCGCCCGCCCTGGAACGTGAACCCCGACGGGCGGACAACATCACTTCTCT
CCATTGCCTCCGGCCGCAGG

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase A; D-alanylalanine synthetase A

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MKEKTRVAILYGGRSAEHDVSRLSAANVLKAIDRTRYEVVPIAISRDGRWRLQPSSEAGADGAGAQVPEDGVEVALLPGG
KGRLVALTKNGEQPDPVDVVFPVLHGPFGEDGSVQGYAEVADVAYVGCGILASAAAMDKDVAKRLLREAGLAVARSVTVL
KGDVGSFQEIAGALGLPFFAKPARQGSSFGVSKVHDRDGFEQAVETALRYDSKALIEEFVDGREIECSVLERADGSLTVS
PPGEIIPADKHGFYTYEAKYFDAEGAVVKVPADVPADVVARTSEMAARAFRALGCEAMARVDFFLRADGSLLVNEVNTLP
GFTDISMYAKALAAAGIGYSKVIDVLIEHALARHRAR

Sequences:

>Translated_357_residues
MKEKTRVAILYGGRSAEHDVSRLSAANVLKAIDRTRYEVVPIAISRDGRWRLQPSSEAGADGAGAQVPEDGVEVALLPGG
KGRLVALTKNGEQPDPVDVVFPVLHGPFGEDGSVQGYAEVADVAYVGCGILASAAAMDKDVAKRLLREAGLAVARSVTVL
KGDVGSFQEIAGALGLPFFAKPARQGSSFGVSKVHDRDGFEQAVETALRYDSKALIEEFVDGREIECSVLERADGSLTVS
PPGEIIPADKHGFYTYEAKYFDAEGAVVKVPADVPADVVARTSEMAARAFRALGCEAMARVDFFLRADGSLLVNEVNTLP
GFTDISMYAKALAAAGIGYSKVIDVLIEHALARHRAR
>Mature_357_residues
MKEKTRVAILYGGRSAEHDVSRLSAANVLKAIDRTRYEVVPIAISRDGRWRLQPSSEAGADGAGAQVPEDGVEVALLPGG
KGRLVALTKNGEQPDPVDVVFPVLHGPFGEDGSVQGYAEVADVAYVGCGILASAAAMDKDVAKRLLREAGLAVARSVTVL
KGDVGSFQEIAGALGLPFFAKPARQGSSFGVSKVHDRDGFEQAVETALRYDSKALIEEFVDGREIECSVLERADGSLTVS
PPGEIIPADKHGFYTYEAKYFDAEGAVVKVPADVPADVVARTSEMAARAFRALGCEAMARVDFFLRADGSLLVNEVNTLP
GFTDISMYAKALAAAGIGYSKVIDVLIEHALARHRAR

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786579, Length=359, Percent_Identity=44.5682451253482, Blast_Score=305, Evalue=4e-84,
Organism=Escherichia coli, GI1786280, Length=352, Percent_Identity=32.9545454545455, Blast_Score=151, Evalue=6e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDLA_RHILO (Q98JS0)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_103309.1
- ProteinModelPortal:   Q98JS0
- SMR:   Q98JS0
- GeneID:   1225970
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll1812
- NMPDR:   fig|266835.1.peg.1413
- HOGENOM:   HBG427092
- OMA:   FTDISMY
- ProtClustDB:   PRK01966
- BRENDA:   6.3.2.4
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 37875; Mature: 37875

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEKTRVAILYGGRSAEHDVSRLSAANVLKAIDRTRYEVVPIAISRDGRWRLQPSSEAGA
CCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCEEECCCCCCCC
DGAGAQVPEDGVEVALLPGGKGRLVALTKNGEQPDPVDVVFPVLHGPFGEDGSVQGYAEV
CCCCCCCCCCCCEEEEEECCCCEEEEEECCCCCCCCHHEEEHHHCCCCCCCCCCCHHHHH
ADVAYVGCGILASAAAMDKDVAKRLLREAGLAVARSVTVLKGDVGSFQEIAGALGLPFFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHEEEEECCCCCHHHHHHHHCCCCCC
KPARQGSSFGVSKVHDRDGFEQAVETALRYDSKALIEEFVDGREIECSVLERADGSLTVS
CHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCEEEHHHHCCCCCEEEC
PPGEIIPADKHGFYTYEAKYFDAEGAVVKVPADVPADVVARTSEMAARAFRALGCEAMAR
CCCCEECCCCCCEEEEEEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCHHHHHH
VDFFLRADGSLLVNEVNTLPGFTDISMYAKALAAAGIGYSKVIDVLIEHALARHRAR
HHHEEECCCCEEEEHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKEKTRVAILYGGRSAEHDVSRLSAANVLKAIDRTRYEVVPIAISRDGRWRLQPSSEAGA
CCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCEEECCCCCCCC
DGAGAQVPEDGVEVALLPGGKGRLVALTKNGEQPDPVDVVFPVLHGPFGEDGSVQGYAEV
CCCCCCCCCCCCEEEEEECCCCEEEEEECCCCCCCCHHEEEHHHCCCCCCCCCCCHHHHH
ADVAYVGCGILASAAAMDKDVAKRLLREAGLAVARSVTVLKGDVGSFQEIAGALGLPFFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHEEEEECCCCCHHHHHHHHCCCCCC
KPARQGSSFGVSKVHDRDGFEQAVETALRYDSKALIEEFVDGREIECSVLERADGSLTVS
CHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCEEEHHHHCCCCCEEEC
PPGEIIPADKHGFYTYEAKYFDAEGAVVKVPADVPADVVARTSEMAARAFRALGCEAMAR
CCCCEECCCCCCEEEEEEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCHHHHHH
VDFFLRADGSLLVNEVNTLPGFTDISMYAKALAAAGIGYSKVIDVLIEHALARHRAR
HHHEEECCCCEEEEHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968