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The map label for this gene is cobA [H]

Identifier: 13471420

GI number: 13471420

Start: 1160992

End: 1161810

Strand: Direct

Name: cobA [H]

Synonym: mlr1386

Alternate gene names: 13471420

Gene position: 1160992-1161810 (Clockwise)

Preceding gene: 13471419

Following gene: 13471421

Centisome position: 16.5

GC content: 69.96

Gene sequence:

>819_bases
GTGAGCGGCACCAGCAAAAACAATGGCGGCAACGCCACGCTCGAACAGGCGCTCGCCCGGCTGAACTTCAAGCCGCGCCC
GCTGGAGCCGGGCCATGTCTGGCTGGCCGGCGCCGGCCCCGGCGACCCCGGCTGCCTGACGCTGGAAGTGCTGGCGGCTT
TGGCCGAAGCGGATGCGCTGGTCTACGACGCGCTGGTCTCGCCGGATGTCGTCGCCGTTGCCGCAAACGCCGAGCTGTTC
TTTGCCGGCAAGCGCGGCGGCCAGCCGTCGATGAAGCAGGATGACATCACCGCTCTGCTGGTCCGGCTGGCGCGTCAGGG
CCGCCGCGTTATCAGGCTCAAGGGCGGCGATCCCTATATTTTCGGTCGTGGCGGCGAAGAAGCCTTGGCGCTCGCACGCG
AAAACATTCCATTCCGCGTGCTGCCGGGCCTGACGTCCGGCCTCAGCGCGCTCGCCGCCACCGGCATCCCGGCCACCATG
CGCGGCATCAACAAGGCGGTGATCCTGGCGACCGGCCACGCCGCCGGCACTGATGACGATATCGACTGGGCGGCGATCGC
CCGCACCGGCCAGCCAGTCGTGGTCTATATGGGCATGGCCAATTTGCCCCTCATCGCCGCAGCACTGCTCGATGGCGGGC
TGTCGCCCTCGACGCCGGCGGCGGTGATCGTCGCCGCCACCACGCCGCAGGAGCGGACCGTCGTCGCCACGCTCGCCACA
ATCGCCGAGGAAGCGGCCGCCGCGGGGCTGACCTCGCCGGCGCTGATCGTCGTCGGCGGCATCGTCGCCATGCGGGCGGC
ATTGACGAGGCAGGCATGA

Upstream 100 bases:

>100_bases
CGATTATCTTCGTCGGCCGCTCGCTGGCGGCGGAAGGTTTTGGCGAAAGTTCATTGTACGACGCCCACTATCAGCGGCGT
TTTCGCGGACGGGACGGATT

Downstream 100 bases:

>100_bases
CGGCCCGCGCCATCATCATCGGCGCGCCGCGCTCCGGCTCGGGCAAGACCAGCGTCACCATCGGCATCTTGCGCGCGCTC
ACCCGGCGCGGCCTGAAAGT

Product: uroporphyrin-III C-methyltransferase/uroporphyrinogen-III synthase

Products: NA

Alternate protein names: Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM [H]

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MSGTSKNNGGNATLEQALARLNFKPRPLEPGHVWLAGAGPGDPGCLTLEVLAALAEADALVYDALVSPDVVAVAANAELF
FAGKRGGQPSMKQDDITALLVRLARQGRRVIRLKGGDPYIFGRGGEEALALARENIPFRVLPGLTSGLSALAATGIPATM
RGINKAVILATGHAAGTDDDIDWAAIARTGQPVVVYMGMANLPLIAAALLDGGLSPSTPAAVIVAATTPQERTVVATLAT
IAEEAAAAGLTSPALIVVGGIVAMRAALTRQA

Sequences:

>Translated_272_residues
MSGTSKNNGGNATLEQALARLNFKPRPLEPGHVWLAGAGPGDPGCLTLEVLAALAEADALVYDALVSPDVVAVAANAELF
FAGKRGGQPSMKQDDITALLVRLARQGRRVIRLKGGDPYIFGRGGEEALALARENIPFRVLPGLTSGLSALAATGIPATM
RGINKAVILATGHAAGTDDDIDWAAIARTGQPVVVYMGMANLPLIAAALLDGGLSPSTPAAVIVAATTPQERTVVATLAT
IAEEAAAAGLTSPALIVVGGIVAMRAALTRQA
>Mature_271_residues
SGTSKNNGGNATLEQALARLNFKPRPLEPGHVWLAGAGPGDPGCLTLEVLAALAEADALVYDALVSPDVVAVAANAELFF
AGKRGGQPSMKQDDITALLVRLARQGRRVIRLKGGDPYIFGRGGEEALALARENIPFRVLPGLTSGLSALAATGIPATMR
GINKAVILATGHAAGTDDDIDWAAIARTGQPVVVYMGMANLPLIAAALLDGGLSPSTPAAVIVAATTPQERTVVATLATI
AEEAAAAGLTSPALIVVGGIVAMRAALTRQA

Specific function: Catalyzes the methylation of both C-2 and C-7 of uroporphyrinogen III leading to precorrin-1 and precorrin-2; their oxidative esterification gives respectively factor I octamethyl ester and sirohydrochlorin [H]

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=265, Percent_Identity=40.377358490566, Blast_Score=186, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6322922, Length=260, Percent_Identity=30.7692307692308, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.107 [H]

Molecular weight: Translated: 27609; Mature: 27478

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: PS00732 RIBOSOMAL_S16 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGTSKNNGGNATLEQALARLNFKPRPLEPGHVWLAGAGPGDPGCLTLEVLAALAEADAL
CCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCEEHHHHHHHHHHHHHH
VYDALVSPDVVAVAANAELFFAGKRGGQPSMKQDDITALLVRLARQGRRVIRLKGGDPYI
HHHHHCCCCEEEEEECCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCEE
FGRGGEEALALARENIPFRVLPGLTSGLSALAATGIPATMRGINKAVILATGHAAGTDDD
EECCCHHHHHHHHCCCCEEECCCCHHHHHHHHHCCCCHHHHCCCEEEEEEECCCCCCCCC
IDWAAIARTGQPVVVYMGMANLPLIAAALLDGGLSPSTPAAVIVAATTPQERTVVATLAT
CCHHEEECCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHH
IAEEAAAAGLTSPALIVVGGIVAMRAALTRQA
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SGTSKNNGGNATLEQALARLNFKPRPLEPGHVWLAGAGPGDPGCLTLEVLAALAEADAL
CCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCEEHHHHHHHHHHHHHH
VYDALVSPDVVAVAANAELFFAGKRGGQPSMKQDDITALLVRLARQGRRVIRLKGGDPYI
HHHHHCCCCEEEEEECCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCEE
FGRGGEEALALARENIPFRVLPGLTSGLSALAATGIPATMRGINKAVILATGHAAGTDDD
EECCCHHHHHHHHCCCCEEECCCCHHHHHHHHHCCCCHHHHCCCEEEEEEECCCCCCCCC
IDWAAIARTGQPVVVYMGMANLPLIAAALLDGGLSPSTPAAVIVAATTPQERTVVATLAT
CCHHEEECCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHH
IAEEAAAAGLTSPALIVVGGIVAMRAALTRQA
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2211520; 2546914 [H]