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The map label for this gene is lipB

Identifier: 13471382

GI number: 13471382

Start: 1120090

End: 1120833

Strand: Direct

Name: lipB

Synonym: mlr1337

Alternate gene names: 13471382

Gene position: 1120090-1120833 (Clockwise)

Preceding gene: 13471381

Following gene: 13471390

Centisome position: 15.92

GC content: 67.47

Gene sequence:

>744_bases
ATGACACAACGCAGCCAGATCGCCACGTCGTTCCTGCCGCTCCCCGGTTCGGCACCGGTCGAGTGGCGCATCGAGCCCGG
CCTTACCGCCTATCCCGATGCGCTCGCCTTCATGGAAGCGCGGGCCGAGGCGATCCGCAGCGGCGGCGCCGGCGAAATGG
TCTGGCTTGTCGAGCATCCCCCGCTTTACACCGCCGGCACCAGCGCCCGCATCGAGGATTTGATCGAGCCCGACCGCTTT
CCCGTCTTTTCGGCCGGCCGCGGCGGCGAATACACCTATCATGGGCCAGGCCAGCGGGTCGCCTATGTCATGCTCGACCT
GAAGCGGCGGCGCGAGGATGTGCGCGCTTTCGTCGCCGCGCTCGAACAGTGGATCATCGGCACGCTCGCCGCCTTCAACG
TGCGCGGCGAACGCCGCGAGGACCGGGTTGGCGTCTGGGTGGTGCGGCCCGATCGCCCTCGCCTGCCGGACGGCTCGCCC
GCCGAGGACAAGATCGCCGCAATAGGCATTAGGCTGCGGCGTTGGGTGAGCTTTCACGGCATCGCCATCAATGTCGAACC
TGAGCTAACCCATTTCGGCGGCATCGTTCCCTGCGGCGTGCAGGACCACGGCGTCACCAGCCTCGTCGACCTCGGACTGC
CCGTCGGCATGGCCGACCTGGATCTGGCGCTGAAATCAGCCTTCGAGGATGTGTTCGGCCCCGTTGCGGCTTTGCCTGCC
GAGGTCGCCCGCAAGACGGGTTGA

Upstream 100 bases:

>100_bases
GACGTTTGCGCGCAATATCGCCATATGGGAGCGATGCATGTCGCCCAAAAACCGCGAGGCGGCCTTGGGCCATGATGTGC
ATAGGCGAAAAAGAAAAACC

Downstream 100 bases:

>100_bases
CCCTTTCGGGCCAAACCAGCTCAAAGCAATTGCGCCGACGCCCACAGAACGCCGCAGCCATGGATGACGAACACCGCCAG
CAGCGCGCCGGCAATGGCGA

Product: lipoyltransferase

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MTQRSQIATSFLPLPGSAPVEWRIEPGLTAYPDALAFMEARAEAIRSGGAGEMVWLVEHPPLYTAGTSARIEDLIEPDRF
PVFSAGRGGEYTYHGPGQRVAYVMLDLKRRREDVRAFVAALEQWIIGTLAAFNVRGERREDRVGVWVVRPDRPRLPDGSP
AEDKIAAIGIRLRRWVSFHGIAINVEPELTHFGGIVPCGVQDHGVTSLVDLGLPVGMADLDLALKSAFEDVFGPVAALPA
EVARKTG

Sequences:

>Translated_247_residues
MTQRSQIATSFLPLPGSAPVEWRIEPGLTAYPDALAFMEARAEAIRSGGAGEMVWLVEHPPLYTAGTSARIEDLIEPDRF
PVFSAGRGGEYTYHGPGQRVAYVMLDLKRRREDVRAFVAALEQWIIGTLAAFNVRGERREDRVGVWVVRPDRPRLPDGSP
AEDKIAAIGIRLRRWVSFHGIAINVEPELTHFGGIVPCGVQDHGVTSLVDLGLPVGMADLDLALKSAFEDVFGPVAALPA
EVARKTG
>Mature_246_residues
TQRSQIATSFLPLPGSAPVEWRIEPGLTAYPDALAFMEARAEAIRSGGAGEMVWLVEHPPLYTAGTSARIEDLIEPDRFP
VFSAGRGGEYTYHGPGQRVAYVMLDLKRRREDVRAFVAALEQWIIGTLAAFNVRGERREDRVGVWVVRPDRPRLPDGSPA
EDKIAAIGIRLRRWVSFHGIAINVEPELTHFGGIVPCGVQDHGVTSLVDLGLPVGMADLDLALKSAFEDVFGPVAALPAE
VARKTG

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Homo sapiens, GI221554520, Length=194, Percent_Identity=33.5051546391753, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI87081767, Length=154, Percent_Identity=42.2077922077922, Blast_Score=132, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6323268, Length=186, Percent_Identity=28.494623655914, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21355483, Length=231, Percent_Identity=32.4675324675325, Blast_Score=98, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_RHILO (Q98KT0)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_102948.1
- ProteinModelPortal:   Q98KT0
- SMR:   Q98KT0
- GeneID:   1225611
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr1337
- NMPDR:   fig|266835.1.peg.1053
- HOGENOM:   HBG716160
- OMA:   DLKRRRP
- BRENDA:   2.3.1.181
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 26933; Mature: 26802

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 198-198

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQRSQIATSFLPLPGSAPVEWRIEPGLTAYPDALAFMEARAEAIRSGGAGEMVWLVEHP
CCCHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECC
PLYTAGTSARIEDLIEPDRFPVFSAGRGGEYTYHGPGQRVAYVMLDLKRRREDVRAFVAA
CEEECCCCHHHHHHCCCCCCCEEECCCCCCEEECCCCCEEHHHHHHHHHHHHHHHHHHHH
LEQWIIGTLAAFNVRGERREDRVGVWVVRPDRPRLPDGSPAEDKIAAIGIRLRRWVSFHG
HHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHEECC
IAINVEPELTHFGGIVPCGVQDHGVTSLVDLGLPVGMADLDLALKSAFEDVFGPVAALPA
EEEEECCCHHHCCCEEECCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EVARKTG
HHHHCCC
>Mature Secondary Structure 
TQRSQIATSFLPLPGSAPVEWRIEPGLTAYPDALAFMEARAEAIRSGGAGEMVWLVEHP
CCHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECC
PLYTAGTSARIEDLIEPDRFPVFSAGRGGEYTYHGPGQRVAYVMLDLKRRREDVRAFVAA
CEEECCCCHHHHHHCCCCCCCEEECCCCCCEEECCCCCEEHHHHHHHHHHHHHHHHHHHH
LEQWIIGTLAAFNVRGERREDRVGVWVVRPDRPRLPDGSPAEDKIAAIGIRLRRWVSFHG
HHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHEECC
IAINVEPELTHFGGIVPCGVQDHGVTSLVDLGLPVGMADLDLALKSAFEDVFGPVAALPA
EEEEECCCHHHCCCEEECCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EVARKTG
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968